Restriction map for
pF1KE0463
(Circular) (Six-base) MAPSORT of: pF1KE0463.seq Check: 9801 from: 1 to: 4361
pF1KE0463 4361 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 929 1327 2186 2618 3365 3633 695
Size: 234 398 859 432 747 268 1423
Fragments arranged by size:
1423 859 747 432 398 268 234
AflIII A'CryG_T
Cuts at: 741 1713 2817 3794 741
Size: 972 1104 977 1308
Fragments arranged by size:
1308 1104 977 972
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AleI CACnn'nnGTG
Cuts at: 1174 1174
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 555 1072 1666 3233 555
Size: 517 594 1567 1683
Fragments arranged by size:
1683 1567 594 517
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 1267 1365 3714 3970 1267
Size: 98 2349 256 1658
Fragments arranged by size:
2349 1658 256 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 1202 1381 1202
Size: 179 4182
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 1216 1377 1791 2039 2074 1216
Size: 161 414 248 35 3503
Fragments arranged by size:
3503 414 248 161 35
BanII G_rGCy'C
Cuts at: 127 443 1375 2405 127
Size: 316 932 1030 2083
Fragments arranged by size:
2083 1030 932 316
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 615 615
Size: 4361
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 1881 1881
Size: 4361
BglI GCCn_nnn'nGGC
Cuts at: 1288 1288
Size: 4361
BglII A'GATC_T
Cuts at: 627 1876 3913 627
Size: 1249 2037 1075
Fragments arranged by size:
2037 1249 1075
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3135 1986
Size: 93 1056 3212
Fragments arranged by size:
3212 1056 93
BmgBI CAC'GTC
Cuts at: 829 829
Size: 4361
BmrI ACTGGGnnnn_n'
Cuts at: 350 1573 1654 1989 4133 350
Size: 1223 81 335 2144 578
Fragments arranged by size:
2144 1223 578 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4361
Bpu10I CC'TnA_GC
Cuts at: 947 947
Size: 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 1537 2416 2908 3206 141
Size: 1396 879 492 298 1296
Fragments arranged by size:
1396 1296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1152 4065 1152
Size: 2913 1448
BsaAI yAC'GTr
Cuts at: 744 1714 744
Size: 970 3391
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 1792 2040 2742 4238 1792
Size: 248 702 1496 1915
Fragments arranged by size:
1915 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3023 3170 3581 1550
Size: 521 678 274 147 411 2330
Fragments arranged by size:
2330 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 677 707 677
Size: 30 4331
BseYI C'CCAG_C
Cuts at: 1209 1786 2544 3121 3617 1209
Size: 577 758 577 496 1953
Fragments arranged by size:
1953 758 577 577 496
BsiEI CG_ry'CG
Cuts at: 104 1224 1903 1949 3157 104
Size: 1120 679 46 1208 1308
Fragments arranged by size:
1308 1208 1120 679 46
BsiHKAI G_wGCw'C
Cuts at: 127 443 1375 2153 2343 3135 127
Size: 316 932 778 190 792 1353
Fragments arranged by size:
1353 932 792 778 316 190
Bsp1286I G_dGCh'C
Cuts at: 127 443 1375 1986 2079 2153 2343 2405 3135
Size: 316 932 611 93 74 190 62 730
Cuts at: 3135 127
Size: 1353
Fragments arranged by size:
1353 932 730 611 316 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3581 3581
Size: 4361
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1411 1625 1927 2308 1411
Size: 214 302 381 3464
Fragments arranged by size:
3464 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1328 2653 2707 4191 4356 50
Size: 1278 1325 54 1484 165 55
Fragments arranged by size:
1484 1325 1278 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2273 2273
Size: 4361
BsrFI r'CCGG_y
Cuts at: 1311 2359 2540 2749 1311
Size: 1048 181 209 2923
Fragments arranged by size:
2923 1048 209 181
BssSI C'ACGA_G
Cuts at: 1228 2632 2990 1228
Size: 1404 358 2599
Fragments arranged by size:
2599 1404 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1738 1738
Size: 4361
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstYI r'GATC_y
Cuts at: 1 627 1386 1853 1876 2211 2457 3469 3555
Size: 626 759 467 23 335 246 1012 86
Cuts at: 3555 3567 3913 1
Size: 12 346 449
Fragments arranged by size:
1012 759 626 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 604 1604 604
Size: 1000 3361
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DraIII CAC_nnn'GTG
Cuts at: 1083 1083
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 1946 2120 2511 2538 3883 1946
Size: 174 391 27 1345 2424
Fragments arranged by size:
2424 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 1240 1343 2384 2594 1240
Size: 103 1041 210 3007
Fragments arranged by size:
3007 1041 210 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 125 441 1373 125
Size: 316 932 3113
Fragments arranged by size:
3113 932 316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 929 1327 2186 2618 3365 3633 695
Size: 234 398 859 432 747 268 1423
Fragments arranged by size:
1423 859 747 432 398 268 234
EcoNI CCTnn'n_nnAGG
Cuts at: 448 448
Size: 4361
EcoO109I rG'GnC_Cy
Cuts at: 1206 1500 1206
Size: 294 4067
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 1795 2043 3065 4044 1795
Size: 248 1022 979 2112
Fragments arranged by size:
2112 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 404 436 2203 2233 2235 2265 205
Size: 32 167 32 1767 30 2 30 2301
Fragments arranged by size:
2301 1767 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1400 1400
Size: 4361
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MfeI C'AATT_G
Cuts at: 880 880
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3032 3216 3032
Size: 184 4177
MscI TGG'CCA
Cuts at: 2122 2122
Size: 4361
MslI CAynn'nnrTG
Cuts at: 575 986 1174 2350 2477 575
Size: 411 188 1176 127 2459
Fragments arranged by size:
2459 1176 411 188 127
MspA1I CmG'CkG
Cuts at: 1472 1786 2146 3159 3404 1472
Size: 314 360 1013 245 2429
Fragments arranged by size:
2429 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1313 2542 1313
Size: 1229 3132
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NgoMIV G'CCGG_C
Cuts at: 1311 2540 1311
Size: 1229 3132
NspI r_CATG'y
Cuts at: 919 1414 1914 2821 919
Size: 495 500 907 2459
Fragments arranged by size:
2459 907 500 495
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PfoI T'CCnGG_A
Cuts at: 443 1240 443
Size: 797 3564
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PpuMI rG'GwC_Cy
Cuts at: 1206 1206
Size: 4361
PsiI TTA'TAA
Cuts at: 619 619
Size: 4361
PstI C_TGCA'G
Cuts at: 1408 2093 1408
Size: 685 3676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 1786 2146 1786
Size: 360 4001
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 127 443 1375 127
Size: 316 932 3113
Fragments arranged by size:
3113 932 316
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SanDI GG'GwC_CC
Cuts at: 1206 1206
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 1240 1343 2384 2594 1240
Size: 103 1041 210 3007
Fragments arranged by size:
3007 1041 210 103
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 695 3878 695
Size: 3183 1178
SfcI C'TryA_G
Cuts at: 33 1404 2089 3082 3273 33
Size: 1371 685 993 191 1121
Fragments arranged by size:
1371 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1204 1383 1204
Size: 179 4182
SmlI C'TyrA_G
Cuts at: 120 1516 2431 2923 3185 120
Size: 1396 915 492 262 1296
Fragments arranged by size:
1396 1296 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 1495 1849 1495
Size: 354 4007
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1944 2077 2536 2745 1944
Size: 133 459 209 3560
Fragments arranged by size:
3560 459 209 133
TatI w'GTAC_w
Cuts at: 693 3876 693
Size: 3183 1178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4329
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1856 1856
Size: 4361
XmaI C'CCGG_G
Cuts at: 1202 1381 1202
Size: 179 4182
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI