Restriction map for
pF1KE0465
(Circular) (Six-base) MAPSORT of: pF1KE0465.seq Check: 1547 from: 1 to: 3770
pF1KE0465 3770 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 2027 2774 3042 1595
Size: 432 747 268 2323
Fragments arranged by size:
2323 747 432 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AleI CACnn'nnGTG
Cuts at: 377 377
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 469 1075 2642 469
Size: 606 1567 1597
Fragments arranged by size:
1597 1567 606
ApaI G_GGCC'C
Cuts at: 564 564
Size: 3770
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 243 774 3123 3379 243
Size: 531 2349 256 634
Fragments arranged by size:
2349 634 531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
AvrII C'CTAG_G
Cuts at: 424 424
Size: 3770
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 313 346 313
Size: 33 3737
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 591 621 651 786 1200 1448 1483 591
Size: 30 30 135 414 248 35 2878
Fragments arranged by size:
2878 414 248 135 35 30 30
BanII G_rGCy'C
Cuts at: 564 784 1814 564
Size: 220 1030 2520
Fragments arranged by size:
2520 1030 220
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BciVI GTATCCnnnnn_n'
Cuts at: 341 1668 2429 3770 341
Size: 1327 761 1341 341
Fragments arranged by size:
1341 1327 761 341
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 500 545 575 605 635 695 882 500
Size: 45 30 30 30 60 187 3388
Fragments arranged by size:
3388 187 60 45 30 30 30
Bme1580I G_kGCm'C
Cuts at: 564 1395 1488 2544 564
Size: 831 93 1056 1790
Fragments arranged by size:
1790 1056 831 93
BmrI ACTGGGnnnn_n'
Cuts at: 265 281 982 1063 1398 3542 265
Size: 16 701 81 335 2144 493
Fragments arranged by size:
2144 701 493 335 81 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
Bpu10I CC'TnA_GC
Cuts at: 390 390
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1825 2317 2615 946
Size: 879 492 298 2101
Fragments arranged by size:
2101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 404 245
Size: 159 3611
BseYI C'CCAG_C
Cuts at: 1195 1953 2530 3026 1195
Size: 758 577 496 1939
Fragments arranged by size:
1939 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 500
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 784 1562 1752 2544 784
Size: 778 190 792 2010
Fragments arranged by size:
2010 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 153 153
Size: 3770
Bsp1286I G_dGCh'C
Cuts at: 564 784 1395 1488 1562 1752 1814 2544 564
Size: 220 611 93 74 190 62 730 1790
Fragments arranged by size:
1790 730 611 220 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1682 1682
Size: 3770
BsrFI r'CCGG_y
Cuts at: 1768 1949 2158 1768
Size: 181 209 3380
Fragments arranged by size:
3380 209 181
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 481 628 1147 481
Size: 147 519 3104
Fragments arranged by size:
3104 519 147
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstEII G'GTnAC_C
Cuts at: 199 320 199
Size: 121 3649
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 378 378
Size: 3770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1013
Size: 3770
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 1355 1529 1920 1947 3292 1355
Size: 174 391 27 1345 1833
Fragments arranged by size:
1833 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 151 1793 2003 151
Size: 1642 210 1918
Fragments arranged by size:
1918 1642 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 782 782
Size: 3770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 2027 2774 3042 1595
Size: 432 747 268 2323
Fragments arranged by size:
2323 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 554 554
Size: 3770
EcoO109I rG'GnC_Cy
Cuts at: 560 561 909 560
Size: 1 348 3421
Fragments arranged by size:
3421 348 1
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1612 1642 1644 1674 1612
Size: 30 2 30 3708
Fragments arranged by size:
3708 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
HindIII A'AGCT_T
Cuts at: 112 112
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 579 639 2441 2625 579
Size: 60 1802 184 1724
Fragments arranged by size:
1802 1724 184 60
MscI TGG'CCA
Cuts at: 1531 1531
Size: 3770
MslI CAynn'nnrTG
Cuts at: 377 1759 1886 377
Size: 1382 127 2261
Fragments arranged by size:
2261 1382 127
MspA1I CmG'CkG
Cuts at: 469 481 499 514 544 574 634 673 694
Size: 12 18 15 30 30 60 39 21
Cuts at: 694 881 1195 1555 2568 2813 469
Size: 187 314 360 1013 245 1426
Fragments arranged by size:
1426 1013 360 314 245 187 60 39
30 30 21 18 15 12
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NspI r_CATG'y
Cuts at: 823 1323 2230 823
Size: 500 907 2363
Fragments arranged by size:
2363 907 500
PasI CC'CwG_GG
Cuts at: 295 295
Size: 3770
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PfoI T'CCnGG_A
Cuts at: 351 351
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PshAI GACnn'nnGTC
Cuts at: 197 197
Size: 3770
PspOMI G'GGCC_C
Cuts at: 560 560
Size: 3770
PstI C_TGCA'G
Cuts at: 474 513 558 588 618 648 678 817 1502
Size: 39 45 30 30 30 30 139 685
Cuts at: 1502 474
Size: 2742
Fragments arranged by size:
2742 685 139 45 39 30 30 30
30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 469 499 514 544 574 634 673 694 1195
Size: 30 15 30 30 60 39 21 501
Cuts at: 1195 1555 469
Size: 360 2684
Fragments arranged by size:
2684 501 360 60 39 30 30 30
21 15
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 784 784
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 470 509 536 554 584 614 644 674
Size: 437 39 27 18 30 30 30 30
Cuts at: 674 704 813 1498 2491 2682 33
Size: 30 109 685 993 191 1121
Fragments arranged by size:
1121 993 685 437 191 109 39 30
30 30 30 30 27 18
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 925 1840 2332 2594 925
Size: 915 492 262 2101
Fragments arranged by size:
2101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
SspI AAT'ATT
Cuts at: 225 225
Size: 3770
StyI C'CwwG_G
Cuts at: 424 904 1258 424
Size: 480 354 2936
Fragments arranged by size:
2936 480 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 1353 1486 1945 2154 218
Size: 1135 133 459 209 1834
Fragments arranged by size:
1834 1135 459 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI