Restriction map for
pF1KE0475
(Circular) (Six-base) MAPSORT of: pF1KE0475.seq Check: 4909 from: 1 to: 3869
pF1KE0475 3869 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 665 907 665
Size: 242 3627
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1694 2126 2873 3141 1694
Size: 432 747 268 2422
Fragments arranged by size:
2422 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AhdI GACnn_n'nnGTC
Cuts at: 661 661
Size: 3869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 273 273
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 1174 2741 1174
Size: 1567 2302
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 384 821 873 3222 3478 384
Size: 437 52 2349 256 775
Fragments arranged by size:
2349 775 437 256 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 200 889 200
Size: 689 3180
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 128 885 1299 1547 1582 128
Size: 757 414 248 35 2415
Fragments arranged by size:
2415 757 414 248 35
BanII G_rGCy'C
Cuts at: 161 883 1913 161
Size: 722 1030 2117
Fragments arranged by size:
2117 1030 722
BbeI G_GCGC'C
Cuts at: 132 1303 1551 132
Size: 1171 248 2450
Fragments arranged by size:
2450 1171 248
BbsI GAAGACnn'nnnn_
Cuts at: 472 472
Size: 3869
BbvCI CC'TCA_GC
Cuts at: 169 169
Size: 3869
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 786 1384 3421 786
Size: 598 2037 1234
Fragments arranged by size:
2037 1234 598
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 292 1494 1587 2643 292
Size: 1202 93 1056 1518
Fragments arranged by size:
1518 1202 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 1081 1162 1497 3641 385
Size: 696 81 335 2144 613
Fragments arranged by size:
2144 696 613 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 698
Size: 3869
Bpu10I CC'TnA_GC
Cuts at: 169 169
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 232 398 3573 232
Size: 166 3175 528
Fragments arranged by size:
3175 528 166
BsaAI yAC'GTr
Cuts at: 1222 1222
Size: 3869
BsaBI GATnn'nnATC
Cuts at: 6 785 1383 3420 6
Size: 779 598 2037 455
Fragments arranged by size:
2037 779 598 455
BsaHI Gr'CG_yC
Cuts at: 129 1300 1548 2250 3746 129
Size: 1171 248 702 1496 252
Fragments arranged by size:
1496 1171 702 252 248
BsaWI w'CCGG_w
Cuts at: 601 1058 1579 2257 2531 2678 3089 601
Size: 457 521 678 274 147 411 1381
Fragments arranged by size:
1381 678 521 457 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 769 196
Size: 573 3296
BseYI C'CCAG_C
Cuts at: 729 1294 2052 2629 3125 729
Size: 565 758 577 496 1473
Fragments arranged by size:
1473 758 577 565 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 521 269
Size: 252 3617
BsiEI CG_ry'CG
Cuts at: 104 1411 1457 2665 104
Size: 1307 46 1208 1308
Fragments arranged by size:
1308 1307 1208 46
BsiHKAI G_wGCw'C
Cuts at: 846 883 1661 1851 2643 846
Size: 37 778 190 792 2072
Fragments arranged by size:
2072 792 778 190 37
Bsp1286I G_dGCh'C
Cuts at: 161 292 846 883 1494 1587 1661 1851 1913
Size: 131 554 37 611 93 74 190 62
Cuts at: 1913 2643 161
Size: 730 1387
Fragments arranged by size:
1387 730 611 554 190 131 93 74
62 37
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 651 919 1133 1435 1816 651
Size: 268 214 302 381 2704
Fragments arranged by size:
2704 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 1867 2048 2257 1867
Size: 181 209 3479
Fragments arranged by size:
3479 209 181
BsrGI T'GTAC_A
Cuts at: 414 414
Size: 3869
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstYI r'GATC_y
Cuts at: 1 501 786 894 1361 1384 1719 1965 2977
Size: 500 285 108 467 23 335 246 1012
Cuts at: 2977 3063 3075 3421 1
Size: 86 12 346 449
Fragments arranged by size:
1012 500 467 449 346 335 285 246
108 86 23 12
BtgI C'CryG_G
Cuts at: 107 233 697 107
Size: 126 464 3279
Fragments arranged by size:
3279 464 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3525
Fragments arranged by size:
3525 181 163
BtsI GCAGTG_nn'
Cuts at: 252 443 538 1112 252
Size: 191 95 574 3009
Fragments arranged by size:
3009 574 191 95
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 1454 1628 2019 2046 3391 1454
Size: 174 391 27 1345 1932
Fragments arranged by size:
1932 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 697 1892 2102 697
Size: 1195 210 2464
Fragments arranged by size:
2464 1195 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2387 2533 3679 2077
Size: 310 146 1146 2267
Fragments arranged by size:
2267 1146 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 1694 2126 2873 3141 698
Size: 996 432 747 268 1426
Fragments arranged by size:
1426 996 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 297 297
Size: 3869
EcoO109I rG'GnC_Cy
Cuts at: 1008 1008
Size: 3869
EcoRI G'AATT_C
Cuts at: 384 873 384
Size: 489 3380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 520 1888 309
Size: 211 1368 2290
Fragments arranged by size:
2290 1368 211
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 132 1303 1551 2573 3552 132
Size: 1171 248 1022 979 449
Fragments arranged by size:
1171 1022 979 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 647 679 1711 1741 1743 1773 647
Size: 32 1032 30 2 30 2743
Fragments arranged by size:
2743 1032 32 30 30 2
HincII GTy'rAC
Cuts at: 666 908 666
Size: 242 3627
KasI G'GCGC_C
Cuts at: 128 1299 1547 128
Size: 1171 248 2450
Fragments arranged by size:
2450 1171 248
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 2540 2724 95
Size: 2445 184 1240
Fragments arranged by size:
2445 1240 184
MscI TGG'CCA
Cuts at: 1630 1630
Size: 3869
MslI CAynn'nnrTG
Cuts at: 1858 1985 1858
Size: 127 3742
MspA1I CmG'CkG
Cuts at: 980 1294 1654 2667 2912 980
Size: 314 360 1013 245 1937
Fragments arranged by size:
1937 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 129 1300 1548 129
Size: 1171 248 2450
Fragments arranged by size:
2450 1171 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3869
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 168 554 922 1422 2329 168
Size: 386 368 500 907 1708
Fragments arranged by size:
1708 907 500 386 368
PacI TTA_AT'TAA
Cuts at: 314 314
Size: 3869
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PfoI T'CCnGG_A
Cuts at: 277 277
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SalI G'TCGA_C
Cuts at: 664 906 664
Size: 242 3627
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 187 3386 187
Size: 3199 670
SfcI C'TryA_G
Cuts at: 33 854 912 1597 2590 2781 33
Size: 821 58 685 993 191 1121
Fragments arranged by size:
1121 993 821 685 191 58
SfoI GGC'GCC
Cuts at: 130 1301 1549 130
Size: 1171 248 2450
Fragments arranged by size:
2450 1171 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 202 891 202
Size: 689 3180
SmlI C'TyrA_G
Cuts at: 1024 1939 2431 2693 1024
Size: 915 492 262 2200
Fragments arranged by size:
2200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 554 922 1422 554
Size: 368 500 3001
Fragments arranged by size:
3001 500 368
SspI AAT'ATT
Cuts at: 393 393
Size: 3869
StyI C'CwwG_G
Cuts at: 107 293 1003 1357 107
Size: 186 710 354 2619
Fragments arranged by size:
2619 710 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1452 1585 2044 2253 1452
Size: 133 459 209 3068
Fragments arranged by size:
3068 459 209 133
TatI w'GTAC_w
Cuts at: 185 414 3384 185
Size: 229 2970 670
Fragments arranged by size:
2970 670 229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 200 889 200
Size: 689 3180
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PacI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI