Restriction map for
pF1KE0476
(Circular) (Six-base) MAPSORT of: pF1KE0476.seq Check: 1520 from: 1 to: 3848
pF1KE0476 3848 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 592 732 592
Size: 140 3708
AccI GT'mk_AC
Cuts at: 614 886 614
Size: 272 3576
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1673 2105 2852 3120 166
Size: 1507 432 747 268 894
Fragments arranged by size:
1507 894 747 432 268
AflIII A'CryG_T
Cuts at: 620 692 1200 2304 3281 620
Size: 72 508 1104 977 1187
Fragments arranged by size:
1187 1104 977 508 72
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaI G_GGCC'C
Cuts at: 122 275 122
Size: 153 3695
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 457 852 3201 3457 457
Size: 395 2349 256 848
Fragments arranged by size:
2349 848 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 304 717 868 304
Size: 413 151 3284
Fragments arranged by size:
3284 413 151
BamHI G'GATC_C
Cuts at: 209 308 873 209
Size: 99 565 3184
Fragments arranged by size:
3184 565 99
BanI G'GyrC_C
Cuts at: 237 399 864 1278 1526 1561 237
Size: 162 465 414 248 35 2524
Fragments arranged by size:
2524 465 414 248 162 35
BanII G_rGCy'C
Cuts at: 122 275 805 862 1892 122
Size: 153 530 57 1030 2078
Fragments arranged by size:
2078 1030 530 153 57
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 807 807
Size: 3848
BbvCI CC'TCA_GC
Cuts at: 733 733
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2507 3848 1746
Size: 761 1341 1746
Fragments arranged by size:
1746 1341 761
BclI T'GATC_A
Cuts at: 518 1368 518
Size: 850 2998
BglI GCCn_nnn'nGGC
Cuts at: 413 413
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 960 960
Size: 3848
Bme1580I G_kGCm'C
Cuts at: 122 240 275 404 1473 1566 2622 122
Size: 118 35 129 1069 93 1056 1348
Fragments arranged by size:
1348 1069 1056 129 118 93 35
BmgBI CAC'GTC
Cuts at: 114 695 114
Size: 581 3267
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 443
Size: 3848
Bpu10I CC'TnA_GC
Cuts at: 733 733
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1903 2395 2693 1024
Size: 879 492 298 2179
Fragments arranged by size:
2179 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 665 1279 1527 2229 3725 665
Size: 614 248 702 1496 788
Fragments arranged by size:
1496 788 702 614 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 766 451
Size: 315 3533
BseYI C'CCAG_C
Cuts at: 121 294 1273 2031 2608 3104 121
Size: 173 979 758 577 496 865
Fragments arranged by size:
979 865 758 577 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 488
Size: 3848
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 571 862 1640 1830 2622 571
Size: 291 778 190 792 1797
Fragments arranged by size:
1797 792 778 291 190
Bsp1286I G_dGCh'C
Cuts at: 122 240 275 404 571 805 862 1473 1566
Size: 118 35 129 167 234 57 611 93
Cuts at: 1566 1640 1830 1892 2622 122
Size: 74 190 62 730 1348
Fragments arranged by size:
1348 730 611 234 190 167 129 118
93 74 62 57 35
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 592 732 898 1112 1414 1795 592
Size: 140 166 214 302 381 2645
Fragments arranged by size:
2645 381 302 214 166 140
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 1760
Size: 3848
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 564 2119 2477 564
Size: 1555 358 1935
Fragments arranged by size:
1935 1555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstEII G'GTnAC_C
Cuts at: 675 675
Size: 3848
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3848
BstYI r'GATC_y
Cuts at: 1 209 308 873 1340 1363 1698 1944 2956
Size: 208 99 565 467 23 335 246 1012
Cuts at: 2956 3042 3054 3400 1
Size: 86 12 346 449
Fragments arranged by size:
1012 565 467 449 346 335 246 208
99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 178 178
Size: 3848
BtgI C'CryG_G
Cuts at: 107 374 410 107
Size: 267 36 3545
Fragments arranged by size:
3545 267 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 351 687 1433 1607 1998 2025 3370 351
Size: 336 746 174 391 27 1345 829
Fragments arranged by size:
1345 829 746 391 336 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 838 1871 2081 838
Size: 1033 210 2605
Fragments arranged by size:
2605 1033 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 443 1673 2105 2852 3120 166
Size: 277 1230 432 747 268 894
Fragments arranged by size:
1230 894 747 432 277 268
EcoO109I rG'GnC_Cy
Cuts at: 118 271 316 379 987 118
Size: 153 45 63 608 2979
Fragments arranged by size:
2979 608 153 63 45
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1690 1720 1722 1752 1690
Size: 30 2 30 3786
Fragments arranged by size:
3786 30 30 2
HincII GTy'rAC
Cuts at: 714 887 714
Size: 173 3675
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 620 3281 620
Size: 2661 1187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 689 1609 689
Size: 920 2928
MslI CAynn'nnrTG
Cuts at: 112 1837 1964 112
Size: 1725 127 1996
Fragments arranged by size:
1996 1725 127
MspA1I CmG'CkG
Cuts at: 662 959 1273 1633 2646 2891 662
Size: 297 314 360 1013 245 1619
Fragments arranged by size:
1619 1013 360 314 297 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 374 410 107
Size: 267 36 3545
Fragments arranged by size:
3545 267 36
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PasI CC'CwG_GG
Cuts at: 214 385 214
Size: 171 3677
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 316 379 316
Size: 63 3785
PspOMI G'GGCC_C
Cuts at: 118 271 118
Size: 153 3695
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 329 361 329
Size: 32 3816
PstI C_TGCA'G
Cuts at: 895 1580 895
Size: 685 3163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 1273 1633 1273
Size: 360 3488
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SanDI GG'GwC_CC
Cuts at: 379 379
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 838 1871 2081 838
Size: 1033 210 2605
Fragments arranged by size:
2605 1033 210
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 891 1576 2569 2760 33
Size: 858 685 993 191 1121
Fragments arranged by size:
1121 993 858 685 191
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 306 870 306
Size: 564 3284
SmlI C'TyrA_G
Cuts at: 1003 1918 2410 2672 1003
Size: 915 492 262 2179
Fragments arranged by size:
2179 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 138 275 374 410 982 1336 107
Size: 31 137 99 36 572 354 2619
Fragments arranged by size:
2619 572 354 137 99 36 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 1431 1564 2023 2232 522
Size: 909 133 459 209 2138
Fragments arranged by size:
2138 909 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 811 1645 811
Size: 834 3014
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 304 868 304
Size: 564 3284
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI