Restriction map for
pF1KE0477
(Circular) (Six-base) MAPSORT of: pF1KE0477.seq Check: 1098 from: 1 to: 4028
pF1KE0477 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 535 747 1044 535
Size: 212 297 3519
Fragments arranged by size:
3519 297 212
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 677 1853 2285 3032 3300 331
Size: 346 1176 432 747 268 1059
Fragments arranged by size:
1176 1059 747 432 346 268
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 895 895
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 913 913
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 151 1333 2900 151
Size: 1182 1567 1279
Fragments arranged by size:
1567 1279 1182
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 590 790 1032 3381 3637 590
Size: 200 242 2349 256 981
Fragments arranged by size:
2349 981 256 242 200
AseI AT'TA_AT
Cuts at: 20 631 20
Size: 611 3417
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 139 535 747 1044 1458 1706 1741 139
Size: 396 212 297 414 248 35 2426
Fragments arranged by size:
2426 414 396 297 248 212 35
BanII G_rGCy'C
Cuts at: 1042 2072 1042
Size: 1030 2998
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 144 1653 1746 2802 144
Size: 1509 93 1056 1370
Fragments arranged by size:
1509 1370 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1204 2083 2575 2873 914
Size: 290 879 492 298 2069
Fragments arranged by size:
2069 879 492 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 890 235
Size: 655 3373
BseYI C'CCAG_C
Cuts at: 144 1453 2211 2788 3284 144
Size: 1309 758 577 496 888
Fragments arranged by size:
1309 888 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 919 197
Size: 722 3306
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1042 1820 2010 2802 1042
Size: 778 190 792 2268
Fragments arranged by size:
2268 792 778 190
BsmI GAATG_Cn'
Cuts at: 338 338
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 144 1042 1653 1746 1820 2010 2072 2802 144
Size: 898 611 93 74 190 62 730 1370
Fragments arranged by size:
1370 898 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 297 672 3204 297
Size: 375 2532 1121
Fragments arranged by size:
2532 1121 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 850 1940 850
Size: 1090 2938
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssHII G'CGCG_C
Cuts at: 113 113
Size: 4028
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstEII G'GTnAC_C
Cuts at: 706 706
Size: 4028
BstYI r'GATC_y
Cuts at: 1 581 1053 1520 1543 1878 2124 3136 3222
Size: 580 472 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 580 472 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 228 1613 1787 2178 2205 3550 228
Size: 1385 174 391 27 1345 706
Fragments arranged by size:
1385 1345 706 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 262 989 2051 2261 262
Size: 727 1062 210 2029
Fragments arranged by size:
2029 1062 727 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 677 1853 2285 3032 3300 331
Size: 346 1176 432 747 268 1059
Fragments arranged by size:
1176 1059 747 432 346 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1018 1018
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 790 1032 790
Size: 242 3786
EcoRV GAT'ATC
Cuts at: 905 905
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 739 871 2047 739
Size: 132 1176 2720
Fragments arranged by size:
2720 1176 132
FspI TGC'GCA
Cuts at: 133 1809 133
Size: 1676 2352
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1870 1900 1902 1932 1870
Size: 30 2 30 3966
Fragments arranged by size:
3966 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 539 751 1048 539
Size: 212 297 3519
Fragments arranged by size:
3519 297 212
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 823 2699 2883 823
Size: 1876 184 1968
Fragments arranged by size:
1968 1876 184
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 343 895 2017 2144 343
Size: 552 1122 127 2227
Fragments arranged by size:
2227 1122 552 127
MspA1I CmG'CkG
Cuts at: 151 1139 1453 1813 2826 3071 151
Size: 988 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 988 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 989 2051 2261 989
Size: 1062 210 2756
Fragments arranged by size:
2756 1062 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 1071 1756 2749 2940 33
Size: 1038 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 191
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 929 1183 2098 2590 2852 929
Size: 254 915 492 262 2105
Fragments arranged by size:
2105 915 492 262 254
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 437 585 1014 1162 1516 437
Size: 148 429 148 354 2949
Fragments arranged by size:
2949 429 354 148 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 271 303 3545 3577 271
Size: 32 3242 32 722
Fragments arranged by size:
3242 722 32 32
Tth111I GACn'n_nGTC
Cuts at: 186 1825 186
Size: 1639 2389
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
XmnI GAAnn'nnTTC
Cuts at: 502 569 502
Size: 67 3961
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI