Restriction map for
pF1KE0481
(Circular) (Six-base) MAPSORT of: pF1KE0481.seq Check: 5936 from: 1 to: 4370
pF1KE0481 4370 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 359 736 1408 359
Size: 377 672 3321
Fragments arranged by size:
3321 672 377
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2627 3374 3642 2195
Size: 432 747 268 2923
Fragments arranged by size:
2923 747 432 268
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1309 1675 3242 1309
Size: 366 1567 2437
Fragments arranged by size:
2437 1567 366
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1115 1374 3723 3979 1115
Size: 259 2349 256 1506
Fragments arranged by size:
2349 1506 259 256
AseI AT'TA_AT
Cuts at: 20 1211 20
Size: 1191 3179
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 446 1390 446
Size: 944 3426
BamHI G'GATC_C
Cuts at: 460 986 1395 460
Size: 526 409 3435
Fragments arranged by size:
3435 526 409
BanI G'GyrC_C
Cuts at: 1386 1800 2048 2083 1386
Size: 414 248 35 3673
Fragments arranged by size:
3673 414 248 35
BanII G_rGCy'C
Cuts at: 161 1280 1384 2414 161
Size: 1119 104 1030 2117
Fragments arranged by size:
2117 1119 1030 104
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BfrBI ATG'CAT
Cuts at: 421 421
Size: 4370
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 1995 2088 3144 1995
Size: 93 1056 3221
Fragments arranged by size:
3221 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 766 790 995 1069 1582 1663 1998 4142 766
Size: 24 205 74 513 81 335 2144 994
Fragments arranged by size:
2144 994 513 335 205 81 74 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1171 2737 1171
Size: 1566 2804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 559 611 546
Size: 13 52 4305
Fragments arranged by size:
4305 52 13
Bpu10I CC'TnA_GC
Cuts at: 548 548
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2917 3215 1546
Size: 879 492 298 2701
Fragments arranged by size:
2701 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 580 4074 580
Size: 3494 876
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 518 802 518
Size: 284 4086
BseYI C'CCAG_C
Cuts at: 1795 2553 3130 3626 1795
Size: 758 577 496 2539
Fragments arranged by size:
2539 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 117 117
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 1912 1958 3166 104
Size: 1808 46 1208 1308
Fragments arranged by size:
1808 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3144 1384
Size: 778 190 792 2610
Fragments arranged by size:
2610 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 161 1280 1384 1995 2088 2162 2352 2414 3144
Size: 1119 104 611 93 74 190 62 730
Cuts at: 3144 161
Size: 1387
Fragments arranged by size:
1387 1119 730 611 190 104 93 74
62
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 203 1420 1634 1936 2317 203
Size: 1217 214 302 381 2256
Fragments arranged by size:
2256 1217 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 295 1188 2368 2549 2758 295
Size: 893 1180 181 209 1907
Fragments arranged by size:
1907 1180 893 209 181
BssSI C'ACGA_G
Cuts at: 1008 2641 2999 1008
Size: 1633 358 2379
Fragments arranged by size:
2379 1633 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstYI r'GATC_y
Cuts at: 1 460 986 1395 1862 1885 2220 2466 3478
Size: 459 526 409 467 23 335 246 1012
Cuts at: 3478 3564 3576 3922 1
Size: 86 12 346 449
Fragments arranged by size:
1012 526 467 459 449 409 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 737 737
Size: 4370
BtgI C'CryG_G
Cuts at: 107 590 107
Size: 483 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 203 795 1074 1613 203
Size: 592 279 539 2960
Fragments arranged by size:
2960 592 539 279
DraI TTT'AAA
Cuts at: 182 1369 182
Size: 1187 3183
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 1955 2129 2520 2547 3892 1955
Size: 174 391 27 1345 2433
Fragments arranged by size:
2433 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1240 2578 2888 3034 4180 1240
Size: 1338 310 146 1146 1430
Fragments arranged by size:
1430 1338 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 559 611 2195 2627 3374 3642 546
Size: 13 52 1584 432 747 268 1274
Fragments arranged by size:
1584 1274 747 432 268 52 13
EcoO109I rG'GnC_Cy
Cuts at: 327 707 745 1024 1509 327
Size: 380 38 279 485 3188
Fragments arranged by size:
3188 485 380 279 38
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 894 2389 894
Size: 1495 2875
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 642 674 1226 1258 2212 2242 2244
Size: 32 165 32 552 32 954 30 2
Cuts at: 2244 2274 445
Size: 30 2541
Fragments arranged by size:
2541 954 552 165 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3225 3041
Size: 184 4186
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 2359 2486 2359
Size: 127 4243
MspA1I CmG'CkG
Cuts at: 1481 1795 2155 3168 3413 1481
Size: 314 360 1013 245 2438
Fragments arranged by size:
2438 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NcoI C'CATG_G
Cuts at: 107 590 107
Size: 483 3887
NdeI CA'TA_TG
Cuts at: 351 351
Size: 4370
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NsiI A_TGCA'T
Cuts at: 423 423
Size: 4370
NspI r_CATG'y
Cuts at: 1423 1923 2830 1423
Size: 500 907 2963
Fragments arranged by size:
2963 907 500
PasI CC'CwG_GG
Cuts at: 581 860 959 581
Size: 279 99 3992
Fragments arranged by size:
3992 279 99
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 327 707 745 1024 327
Size: 380 38 279 3673
Fragments arranged by size:
3673 380 279 38
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 901 933 901
Size: 32 4338
PstI C_TGCA'G
Cuts at: 744 1417 2102 744
Size: 673 685 3012
Fragments arranged by size:
3012 685 673
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 740 1413 2098 3091 3282 33
Size: 707 673 685 993 191 1121
Fragments arranged by size:
1121 993 707 685 673 191
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 448 1392 448
Size: 944 3426
SmlI C'TyrA_G
Cuts at: 1525 2440 2932 3194 1525
Size: 915 492 262 2701
Fragments arranged by size:
2701 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 107 590 1504 1858 107
Size: 483 914 354 2619
Fragments arranged by size:
2619 914 483 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 1064 1953 2086 2545 2754 712
Size: 352 889 133 459 209 2328
Fragments arranged by size:
2328 889 459 352 209 133
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 796 1075 2167 796
Size: 279 1092 2999
Fragments arranged by size:
2999 1092 279
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 588 1865 588
Size: 1277 3093
XmaI C'CCGG_G
Cuts at: 446 1390 446
Size: 944 3426
XmnI GAAnn'nnTTC
Cuts at: 1220 1220
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI