Restriction map for
pF1KE0484
(Circular) (Six-base) MAPSORT of: pF1KE0484.seq Check: 6933 from: 1 to: 3791
pF1KE0484 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 440 807 440
Size: 367 3424
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1616 2048 2795 3063 650
Size: 966 432 747 268 1378
Fragments arranged by size:
1378 966 747 432 268
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AhdI GACnn_n'nnGTC
Cuts at: 352 615 352
Size: 263 3528
AlwNI CAG_nnn'CTG
Cuts at: 489 1096 2663 489
Size: 607 1567 1617
Fragments arranged by size:
1617 1567 607
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 314 795 3144 3400 314
Size: 481 2349 256 705
Fragments arranged by size:
2349 705 481 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 440 807 1221 1469 1504 440
Size: 367 414 248 35 2727
Fragments arranged by size:
2727 414 367 248 35
BanII G_rGCy'C
Cuts at: 497 805 1835 497
Size: 308 1030 2453
Fragments arranged by size:
2453 1030 308
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 638 638
Size: 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 653 1306 3343 653
Size: 653 2037 1101
Fragments arranged by size:
2037 1101 653
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmgBI CAC'GTC
Cuts at: 129 129
Size: 3791
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 511 454
Size: 57 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 452 482 452
Size: 30 3761
BseYI C'CCAG_C
Cuts at: 719 1216 1974 2551 3047 719
Size: 497 758 577 496 1463
Fragments arranged by size:
1463 758 577 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 571
Size: 3791
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 497 805 1416 1509 1583 1773 1835 2565 497
Size: 308 611 93 74 190 62 730 1723
Fragments arranged by size:
1723 730 611 308 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 841 1055 1357 1738 632
Size: 209 214 302 381 2685
Fragments arranged by size:
2685 381 302 214 209
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 701 1789 1970 2179 701
Size: 1088 181 209 2313
Fragments arranged by size:
2313 1088 209 181
BsrGI T'GTAC_A
Cuts at: 569 569
Size: 3791
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 729 1168 729
Size: 439 3352
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 3791
BstYI r'GATC_y
Cuts at: 1 653 708 816 1283 1306 1641 1887 2899
Size: 652 55 108 467 23 335 246 1012
Cuts at: 2899 2985 2997 3343 1
Size: 86 12 346 449
Fragments arranged by size:
1012 652 467 449 346 335 246 108
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 134 1741 1922 2085 134
Size: 1607 181 163 1840
Fragments arranged by size:
1840 1607 181 163
BtsI GCAGTG_nn'
Cuts at: 554 1034 554
Size: 480 3311
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 1376 1550 1941 1968 3313 1376
Size: 174 391 27 1345 1854
Fragments arranged by size:
1854 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 209 1814 2024 209
Size: 1605 210 1976
Fragments arranged by size:
1976 1605 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 511 650 1616 2048 2795 3063 454
Size: 57 139 966 432 747 268 1182
Fragments arranged by size:
1182 966 747 432 268 139 57
EcoO109I rG'GnC_Cy
Cuts at: 930 930
Size: 3791
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 256 1810 256
Size: 1554 2237
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 370 452 484 601 633 638 670 1633
Size: 32 82 32 117 32 5 32 963
Cuts at: 1633 1663 1665 1695 338
Size: 30 2 30 2434
Fragments arranged by size:
2434 963 117 82 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 444 811 444
Size: 367 3424
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 2462 2646 710
Size: 1752 184 1855
Fragments arranged by size:
1855 1752 184
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 703 1972 703
Size: 1269 2522
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NdeI CA'TA_TG
Cuts at: 149 149
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 701 1970 701
Size: 1269 2522
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PflMI CCAn_nnn'nTGG
Cuts at: 489 489
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 549 838 1523 549
Size: 289 685 2817
Fragments arranged by size:
2817 685 289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SexAI A'CCwGG_T
Cuts at: 436 436
Size: 3791
SfcI C'TryA_G
Cuts at: 33 545 834 1519 2512 2703 33
Size: 512 289 685 993 191 1121
Fragments arranged by size:
1121 993 685 512 289 191
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
SspI AAT'ATT
Cuts at: 274 274
Size: 3791
StyI C'CwwG_G
Cuts at: 107 925 1279 107
Size: 818 354 2619
Fragments arranged by size:
2619 818 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 569 3306 569
Size: 2737 1054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI