Restriction map for
pF1KE0488
(Circular) (Six-base) MAPSORT of: pF1KE0488.seq Check: 7180 from: 1 to: 4220
pF1KE0488 4220 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1258 1258
Size: 4220
Acc65I G'GTAC_C
Cuts at: 569 1236 569
Size: 667 3553
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2045 2477 3224 3492 2045
Size: 432 747 268 2773
Fragments arranged by size:
2773 747 432 268
AfeI AGC'GCT
Cuts at: 514 514
Size: 4220
AflIII A'CryG_T
Cuts at: 422 911 1572 2676 3653 422
Size: 489 661 1104 977 989
Fragments arranged by size:
1104 989 977 661 489
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AhdI GACnn_n'nnGTC
Cuts at: 143 143
Size: 4220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 940
Size: 4220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 674 706 674
Size: 32 4188
AlwNI CAG_nnn'CTG
Cuts at: 483 836 1525 3092 483
Size: 353 689 1567 1611
Fragments arranged by size:
1611 1567 689 353
ApaLI G'TGCA_C
Cuts at: 2990 2990
Size: 4220
ApoI r'AATT_y
Cuts at: 1224 3573 3829 1224
Size: 2349 256 1615
Fragments arranged by size:
2349 1615 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 132 193 601 647 1240 132
Size: 61 408 46 593 3112
Fragments arranged by size:
3112 593 408 61 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 841 874 841
Size: 33 4187
BamHI G'GATC_C
Cuts at: 1245 1245
Size: 4220
BanI G'GyrC_C
Cuts at: 117 569 1236 1650 1898 1933 117
Size: 452 667 414 248 35 2404
Fragments arranged by size:
2404 667 452 414 248 35
BanII G_rGCy'C
Cuts at: 934 1040 1234 2264 934
Size: 106 194 1030 2890
Fragments arranged by size:
2890 1030 194 106
BbeI G_GCGC'C
Cuts at: 121 1654 1902 121
Size: 1533 248 2439
Fragments arranged by size:
2439 1533 248
BbsI GAAGACnn'nnnn_
Cuts at: 600 600
Size: 4220
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 1740 1740
Size: 4220
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 928 1332 928
Size: 404 3816
Bme1580I G_kGCm'C
Cuts at: 850 1845 1938 2994 850
Size: 995 93 1056 2076
Fragments arranged by size:
2076 1056 995 93
BmrI ACTGGGnnnn_n'
Cuts at: 161 1035 1432 1513 1848 3992 161
Size: 874 397 81 335 2144 389
Fragments arranged by size:
2144 874 397 389 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 231
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2275 2767 3065 1396
Size: 879 492 298 2551
Fragments arranged by size:
2551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 709 3924 709
Size: 3215 1005
BsaAI yAC'GTr
Cuts at: 339 423 1573 339
Size: 84 1150 2986
Fragments arranged by size:
2986 1150 84
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 118 1651 1899 2601 4097 118
Size: 1533 248 702 1496 241
Fragments arranged by size:
1533 1496 702 248 241
BsaWI w'CCGG_w
Cuts at: 1409 1930 2608 2882 3029 3440 1409
Size: 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 778 1046 1645 2403 2980 3476 778
Size: 268 599 758 577 496 1522
Fragments arranged by size:
1522 758 599 577 496 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 438
Size: 4220
BsiEI CG_ry'CG
Cuts at: 104 1175 1762 1808 3016 104
Size: 1071 587 46 1208 1308
Fragments arranged by size:
1308 1208 1071 587 46
BsiHKAI G_wGCw'C
Cuts at: 956 1234 2012 2202 2994 956
Size: 278 778 190 792 2182
Fragments arranged by size:
2182 792 778 278 190
BsmBI CGTCTCn'nnnn_
Cuts at: 372 372
Size: 4220
Bsp1286I G_dGCh'C
Cuts at: 850 934 956 1040 1234 1845 1938 2012 2202
Size: 84 22 84 194 611 93 74 190
Cuts at: 2202 2264 2994 850
Size: 62 730 2076
Fragments arranged by size:
2076 730 611 194 190 93 84 84
74 62 22
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 503 632 1270 1484 1786 2167 503
Size: 129 638 214 302 381 2556
Fragments arranged by size:
2556 638 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 2512 2566 4050 4215 50
Size: 2462 54 1484 165 55
Fragments arranged by size:
2462 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2132 2132
Size: 4220
BsrFI r'CCGG_y
Cuts at: 250 566 581 2218 2399 2608 250
Size: 316 15 1637 181 209 1862
Fragments arranged by size:
1862 1637 316 209 181 15
BssSI C'ACGA_G
Cuts at: 2491 2849 2491
Size: 358 3862
BstAPI GCAn_nnn'nTGC
Cuts at: 983 1597 983
Size: 614 3606
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstYI r'GATC_y
Cuts at: 1 1245 1712 1735 2070 2316 3328 3414 3426
Size: 1244 467 23 335 246 1012 86 12
Cuts at: 3426 3772 1
Size: 346 449
Fragments arranged by size:
1244 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 232 232
Size: 4220
BtgI C'CryG_G
Cuts at: 107 850 1126 107
Size: 743 276 3201
Fragments arranged by size:
3201 743 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 2170 2351 2514 228
Size: 1942 181 163 1934
Fragments arranged by size:
1942 1934 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DrdI GACnn_nn'nnGTC
Cuts at: 1926 2784 1926
Size: 858 3362
EaeI y'GGCC_r
Cuts at: 1172 1805 1979 2370 2397 3742 1172
Size: 633 174 391 27 1345 1650
Fragments arranged by size:
1650 1345 633 391 174 27
EagI C'GGCC_G
Cuts at: 1172 1805 1172
Size: 633 3587
EarI CTCTTCn'nnn_
Cuts at: 322 2243 2453 322
Size: 1921 210 2089
Fragments arranged by size:
2089 1921 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2428 2738 2884 4030 2428
Size: 310 146 1146 2618
Fragments arranged by size:
2618 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1232 1232
Size: 4220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 2045 2477 3224 3492 231
Size: 1814 432 747 268 959
Fragments arranged by size:
1814 959 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 385 385
Size: 4220
EcoO109I rG'GnC_Cy
Cuts at: 127 468 1359 127
Size: 341 891 2988
Fragments arranged by size:
2988 891 341
EcoRI G'AATT_C
Cuts at: 1224 1224
Size: 4220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 4220
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 121 516 1062 1654 1902 2924 3903 121
Size: 395 546 592 248 1022 979 438
Fragments arranged by size:
1022 979 592 546 438 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 129 161 2062 2092 2094 2124 129
Size: 32 1901 30 2 30 2225
Fragments arranged by size:
2225 1901 32 30 30 2
HincII GTy'rAC
Cuts at: 375 1259 375
Size: 884 3336
HindIII A'AGCT_T
Cuts at: 728 728
Size: 4220
KasI G'GCGC_C
Cuts at: 117 1650 1898 117
Size: 1533 248 2439
Fragments arranged by size:
2439 1533 248
KpnI G_GTAC'C
Cuts at: 573 1240 573
Size: 667 3553
MluI A'CGCG_T
Cuts at: 911 3653 911
Size: 2742 1478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2891 3075 2891
Size: 184 4036
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 295 922 2209 2336 295
Size: 627 1287 127 2179
Fragments arranged by size:
2179 1287 627 127
MspA1I CmG'CkG
Cuts at: 286 782 1171 1331 1645 2005 3018 3263 286
Size: 496 389 160 314 360 1013 245 1243
Fragments arranged by size:
1243 1013 496 389 360 314 245 160
NaeI GCC'GGC
Cuts at: 583 2401 583
Size: 1818 2402
NarI GG'CG_CC
Cuts at: 118 1651 1899 118
Size: 1533 248 2439
Fragments arranged by size:
2439 1533 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4220
NgoMIV G'CCGG_C
Cuts at: 581 2399 581
Size: 1818 2402
NspI r_CATG'y
Cuts at: 1093 1273 1773 2680 1093
Size: 180 500 907 2633
Fragments arranged by size:
2633 907 500 180
PasI CC'CwG_GG
Cuts at: 934 934
Size: 4220
PciI A'CATG_T
Cuts at: 2676 2676
Size: 4220
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4220
PfoI T'CCnGG_A
Cuts at: 1206 1206
Size: 4220
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PmlI CAC'GTG
Cuts at: 339 339
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 4188
PpuMI rG'GwC_Cy
Cuts at: 127 127
Size: 4220
PspXI vC'TCGA_Gb
Cuts at: 193 193
Size: 4220
PstI C_TGCA'G
Cuts at: 413 500 745 1267 1952 413
Size: 87 245 522 685 2681
Fragments arranged by size:
2681 685 522 245 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 1645 2005 1645
Size: 360 3860
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 1234 1234
Size: 4220
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SanDI GG'GwC_CC
Cuts at: 127 127
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 500 1267 500
Size: 767 3453
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 409 496 741 837 1263 1948 2941 3132
Size: 376 87 245 96 426 685 993 191
Cuts at: 3132 33
Size: 1121
Fragments arranged by size:
1121 993 685 426 376 245 191 96
87
SfoI GGC'GCC
Cuts at: 119 1652 1900 119
Size: 1533 248 2439
Fragments arranged by size:
2439 1533 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 649 1242 649
Size: 593 3627
SmlI C'TyrA_G
Cuts at: 193 1375 2290 2782 3044 193
Size: 1182 915 492 262 1369
Fragments arranged by size:
1369 1182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1093 1273 1773 1093
Size: 180 500 3540
Fragments arranged by size:
3540 500 180
StyI C'CwwG_G
Cuts at: 107 122 1354 1708 107
Size: 15 1232 354 2619
Fragments arranged by size:
2619 1232 354 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 1803 1936 2395 2604 472
Size: 1331 133 459 209 2088
Fragments arranged by size:
2088 1331 459 209 133
TatI w'GTAC_w
Cuts at: 425 3735 425
Size: 3310 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4188
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 1715
Size: 4220
XhoI C'TCGA_G
Cuts at: 193 193
Size: 4220
XmaI C'CCGG_G
Cuts at: 647 1240 647
Size: 593 3627
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI ApaI AscI AvrII BbvCI
BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspOMI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI