Restriction map for
pF1KE0490
(Circular) (Six-base) MAPSORT of: pF1KE0490.seq Check: 8692 from: 1 to: 4334
pF1KE0490 4334 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 320 586 1191 320
Size: 266 605 3463
Fragments arranged by size:
3463 605 266
AatII G_ACGT'C
Cuts at: 728 728
Size: 4334
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AfeI AGC'GCT
Cuts at: 343 343
Size: 4334
AflIII A'CryG_T
Cuts at: 431 1686 2790 3767 431
Size: 1255 1104 977 998
Fragments arranged by size:
1255 1104 998 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 1229 1229
Size: 4334
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 1324 1338 3687 3943 1324
Size: 14 2349 256 1715
Fragments arranged by size:
2349 1715 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 190 815 1354 190
Size: 625 539 3170
Fragments arranged by size:
3170 625 539
AvrII C'CTAG_G
Cuts at: 507 507
Size: 4334
BamHI G'GATC_C
Cuts at: 138 1359 138
Size: 1221 3113
BanI G'GyrC_C
Cuts at: 442 574 970 1243 1279 1350 1764 2012 2047
Size: 132 396 273 36 71 414 248 35
Cuts at: 2047 442
Size: 2729
Fragments arranged by size:
2729 414 396 273 248 132 71 36
35
BanII G_rGCy'C
Cuts at: 1229 1348 2378 1229
Size: 119 1030 3185
Fragments arranged by size:
3185 1030 119
BbeI G_GCGC'C
Cuts at: 1247 1283 1768 2016 1247
Size: 36 485 248 3565
Fragments arranged by size:
3565 485 248 36
BbsI GAAGACnn'nnnn_
Cuts at: 1303 1303
Size: 4334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 149 183 149
Size: 34 4300
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 247 1233 1277 247
Size: 986 44 3304
Fragments arranged by size:
3304 986 44
BglII A'GATC_T
Cuts at: 299 1849 3886 299
Size: 1550 2037 747
Fragments arranged by size:
2037 1550 747
BlpI GC'TnA_GC
Cuts at: 397 826 1309 1446 397
Size: 429 483 137 3285
Fragments arranged by size:
3285 483 429 137
Bme1580I G_kGCm'C
Cuts at: 374 973 1101 1229 1959 2052 3108 374
Size: 599 128 128 730 93 1056 1600
Fragments arranged by size:
1600 1056 730 599 128 128 93
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1627 1962 4106 1546
Size: 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 934 1101 1237 673
Size: 261 167 136 3770
Fragments arranged by size:
3770 261 167 136
Bpu10I CC'TnA_GC
Cuts at: 245 487 245
Size: 242 4092
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1111 1510 2389 2881 3179 832
Size: 279 399 879 492 298 1987
Fragments arranged by size:
1987 879 492 399 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 1126 4038 1126
Size: 2912 1422
BsaAI yAC'GTr
Cuts at: 432 747 1687 432
Size: 315 940 3079
Fragments arranged by size:
3079 940 315
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 725 1163 1244 1280 1765 2013 2715 4211 725
Size: 438 81 36 485 248 702 1496 848
Fragments arranged by size:
1496 848 702 485 438 248 81 36
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 751 781 1240 1270 751
Size: 30 459 30 3815
Fragments arranged by size:
3815 459 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 527 394
Size: 133 4201
BseYI C'CCAG_C
Cuts at: 172 1759 2517 3094 3590 172
Size: 1587 758 577 496 916
Fragments arranged by size:
1587 916 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 4334
BsiEI CG_ry'CG
Cuts at: 104 158 1876 1922 3130 104
Size: 54 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 54 46
BsiHKAI G_wGCw'C
Cuts at: 1348 2126 2316 3108 1348
Size: 778 190 792 2574
Fragments arranged by size:
2574 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 183 183
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 374 973 1101 1229 1348 1959 2052 2126 2316
Size: 599 128 128 119 611 93 74 190
Cuts at: 2316 2378 3108 374
Size: 62 730 1600
Fragments arranged by size:
1600 730 611 599 190 128 128 119
93 74 62
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 586 1191 1384 1598 1900 2281 320
Size: 266 605 193 214 302 381 2373
Fragments arranged by size:
2373 605 381 302 266 214 193
BsrBI CCG'CTC
Cuts at: 50 244 1262 2626 2680 4164 4329 50
Size: 194 1018 1364 54 1484 165 55
Fragments arranged by size:
1484 1364 1018 194 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 982 2332 2513 2722 982
Size: 1350 181 209 2594
Fragments arranged by size:
2594 1350 209 181
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 581 897 1711 581
Size: 316 814 3204
Fragments arranged by size:
3204 814 316
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 210 210
Size: 4334
BstYI r'GATC_y
Cuts at: 1 138 299 1359 1826 1849 2184 2430 3442
Size: 137 161 1060 467 23 335 246 1012
Cuts at: 3442 3528 3540 3886 1
Size: 86 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 246 161
137 86 23 12
BtgI C'CryG_G
Cuts at: 209 974 1248 209
Size: 765 274 3295
Fragments arranged by size:
3295 765 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1577 203
Size: 1374 2960
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 724 2040 2898 724
Size: 1316 858 2160
Fragments arranged by size:
2160 1316 858
EaeI y'GGCC_r
Cuts at: 905 1919 2093 2484 2511 3856 905
Size: 1014 174 391 27 1345 1383
Fragments arranged by size:
1383 1345 1014 391 174 27
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 367 412 799 805 1102 2357 2567 367
Size: 45 387 6 297 1255 210 2134
Fragments arranged by size:
2134 1255 387 297 210 45 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1346 1346
Size: 4334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 934 1101 1237 2159 2591 3338 3606 673
Size: 261 167 136 922 432 747 268 1401
Fragments arranged by size:
1401 922 747 432 268 261 167 136
EcoO109I rG'GnC_Cy
Cuts at: 1473 1473
Size: 4334
EcoRI G'AATT_C
Cuts at: 1324 1338 1324
Size: 14 4320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 355 2115 355
Size: 1760 2574
FspAI rTGC'GCAy
Cuts at: 355 355
Size: 4334
HaeII r_GCGC'y
Cuts at: 345 1247 1283 1768 2016 3038 4017 345
Size: 902 36 485 248 1022 979 662
Fragments arranged by size:
1022 979 902 662 485 248 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 2176 2206 2208 2238 130
Size: 32 2014 30 2 30 2226
Fragments arranged by size:
2226 2014 32 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1243 1279 1764 2012 1243
Size: 36 485 248 3565
Fragments arranged by size:
3565 485 248 36
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 512 3005 3189 512
Size: 2493 184 1657
Fragments arranged by size:
2493 1657 184
MscI TGG'CCA
Cuts at: 907 2095 907
Size: 1188 3146
MslI CAynn'nnrTG
Cuts at: 112 208 1006 2323 2450 112
Size: 96 798 1317 127 1996
Fragments arranged by size:
1996 1317 798 127 96
MspA1I CmG'CkG
Cuts at: 326 563 1094 1445 1759 2119 3132 3377 326
Size: 237 531 351 314 360 1013 245 1283
Fragments arranged by size:
1283 1013 531 360 351 314 245 237
NaeI GCC'GGC
Cuts at: 984 2515 984
Size: 1531 2803
NarI GG'CG_CC
Cuts at: 1244 1280 1765 2013 1244
Size: 36 485 248 3565
Fragments arranged by size:
3565 485 248 36
NcoI C'CATG_G
Cuts at: 209 209
Size: 4334
NgoMIV G'CCGG_C
Cuts at: 982 2513 982
Size: 1531 2803
NspI r_CATG'y
Cuts at: 293 309 900 1387 1887 2794 293
Size: 16 591 487 500 907 1833
Fragments arranged by size:
1833 907 591 500 487 16
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PfoI T'CCnGG_A
Cuts at: 911 911
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PspOMI G'GGCC_C
Cuts at: 1225 1225
Size: 4334
PstI C_TGCA'G
Cuts at: 644 1381 2066 644
Size: 737 685 2912
Fragments arranged by size:
2912 737 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 326 563 1759 2119 326
Size: 237 1196 360 2541
Fragments arranged by size:
2541 1196 360 237
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 1348 1348
Size: 4334
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 1102 2357 2567 1102
Size: 1255 210 2869
Fragments arranged by size:
2869 1255 210
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 427 3851 427
Size: 3424 910
SfcI C'TryA_G
Cuts at: 33 321 640 834 1377 2062 3055 3246 33
Size: 288 319 194 543 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 319 288 194 191
SfoI GGC'GCC
Cuts at: 1245 1281 1766 2014 1245
Size: 36 485 248 3565
Fragments arranged by size:
3565 485 248 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SmaI CCC'GGG
Cuts at: 817 1356 817
Size: 539 3795
SmlI C'TyrA_G
Cuts at: 847 1126 1489 2404 2896 3158 847
Size: 279 363 915 492 262 2023
Fragments arranged by size:
2023 915 492 363 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 900 1387 1887 900
Size: 487 500 3347
Fragments arranged by size:
3347 500 487
StuI AGG'CCT
Cuts at: 466 694 466
Size: 228 4106
StyI C'CwwG_G
Cuts at: 209 467 507 1468 1822 209
Size: 258 40 961 354 2721
Fragments arranged by size:
2721 961 354 258 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1917 2050 2509 2718 1917
Size: 133 459 209 3533
Fragments arranged by size:
3533 459 209 133
TatI w'GTAC_w
Cuts at: 425 434 1196 3849 425
Size: 9 762 2653 910
Fragments arranged by size:
2653 910 762 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1240 1272 3851 3883 1240
Size: 32 2579 32 1691
Fragments arranged by size:
2579 1691 32 32
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1273 1829 1273
Size: 556 3778
XmaI C'CCGG_G
Cuts at: 815 1354 815
Size: 539 3795
ZraI GAC'GTC
Cuts at: 726 726
Size: 4334
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI BaeI BbvCI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI