Restriction map for
pF1KE0491
(Circular) (Six-base) MAPSORT of: pF1KE0491.seq Check: 3529 from: 1 to: 4082
pF1KE0491 4082 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 1098 1098
Size: 4082
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 961 1907 2339 3086 3354 153
Size: 808 946 432 747 268 881
Fragments arranged by size:
946 881 808 747 432 268
AflIII A'CryG_T
Cuts at: 704 1434 2538 3515 704
Size: 730 1104 977 1271
Fragments arranged by size:
1271 1104 977 730
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaI G_GGCC'C
Cuts at: 660 660
Size: 4082
ApaLI G'TGCA_C
Cuts at: 700 2852 700
Size: 2152 1930
ApoI r'AATT_y
Cuts at: 321 1086 3435 3691 321
Size: 765 2349 256 712
Fragments arranged by size:
2349 765 712 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 1102 1102
Size: 4082
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 996 1029 996
Size: 33 4049
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 1098 1512 1760 1795 1098
Size: 414 248 35 3385
Fragments arranged by size:
3385 414 248 35
BanII G_rGCy'C
Cuts at: 660 845 1096 2126 660
Size: 185 251 1030 2616
Fragments arranged by size:
2616 1030 251 185
BbeI G_GCGC'C
Cuts at: 1516 1764 1516
Size: 248 3834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 513 547 513
Size: 34 4048
BciVI GTATCCnnnnn_n'
Cuts at: 1024 1980 2741 4082 1024
Size: 956 761 1341 1024
Fragments arranged by size:
1341 1024 956 761
BclI T'GATC_A
Cuts at: 348 1602 348
Size: 1254 2828
BglI GCCn_nnn'nGGC
Cuts at: 412 412
Size: 4082
BglII A'GATC_T
Cuts at: 908 1597 3634 908
Size: 689 2037 1356
Fragments arranged by size:
2037 1356 689
BlpI GC'TnA_GC
Cuts at: 925 1194 925
Size: 269 3813
Bme1580I G_kGCm'C
Cuts at: 660 704 1707 1800 2856 660
Size: 44 1003 93 1056 1886
Fragments arranged by size:
1886 1056 1003 93 44
BmrI ACTGGGnnnn_n'
Cuts at: 1294 1375 1710 3854 1294
Size: 81 335 2144 1522
Fragments arranged by size:
2144 1522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 402 745 807 837 982 198
Size: 204 343 62 30 145 3298
Fragments arranged by size:
3298 343 204 145 62 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2137 2629 2927 1258
Size: 879 492 298 2413
Fragments arranged by size:
2413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 587 3786 587
Size: 3199 883
BsaAI yAC'GTr
Cuts at: 1435 1435
Size: 4082
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 860 1513 1761 2463 3959 860
Size: 653 248 702 1496 983
Fragments arranged by size:
1496 983 702 653 248
BsaWI w'CCGG_w
Cuts at: 289 1271 1792 2470 2744 2891 3302 289
Size: 982 521 678 274 147 411 1069
Fragments arranged by size:
1069 982 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 787 1507 2265 2842 3338 787
Size: 720 758 577 496 1531
Fragments arranged by size:
1531 758 720 577 496
BsiEI CG_ry'CG
Cuts at: 104 717 1624 1670 2878 104
Size: 613 907 46 1208 1308
Fragments arranged by size:
1308 1208 907 613 46
BsiHKAI G_wGCw'C
Cuts at: 704 1096 1874 2064 2856 704
Size: 392 778 190 792 1930
Fragments arranged by size:
1930 792 778 392 190
Bsp1286I G_dGCh'C
Cuts at: 660 704 845 1096 1707 1800 1874 2064 2126
Size: 44 141 251 611 93 74 190 62
Cuts at: 2126 2856 660
Size: 730 1886
Fragments arranged by size:
1886 730 611 251 190 141 93 74
62 44
BspEI T'CCGG_A
Cuts at: 289 3302 289
Size: 3013 1069
BspHI T'CATG_A
Cuts at: 3258 3258
Size: 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 313 532 1132 1346 1648 2029 313
Size: 219 600 214 302 381 2366
Fragments arranged by size:
2366 600 381 302 219 214
BsrBI CCG'CTC
Cuts at: 50 821 2374 2428 3912 4077 50
Size: 771 1553 54 1484 165 55
Fragments arranged by size:
1553 1484 771 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1994 1994
Size: 4082
BsrFI r'CCGG_y
Cuts at: 520 716 2080 2261 2470 520
Size: 196 1364 181 209 2132
Fragments arranged by size:
2132 1364 209 196 181
BsrGI T'GTAC_A
Cuts at: 572 917 572
Size: 345 3737
BssSI C'ACGA_G
Cuts at: 2353 2711 2353
Size: 358 3724
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 189 189
Size: 4082
BstYI r'GATC_y
Cuts at: 1 908 1107 1574 1597 1932 2178 3190 3276
Size: 907 199 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 907 467 449 346 335 246 199
86 23 12
BtgI C'CryG_G
Cuts at: 107 737 749 836 869 902 107
Size: 630 12 87 33 33 3287
Fragments arranged by size:
3287 630 87 33 33 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 456 657 2032 2213 2376 456
Size: 201 1375 181 163 2162
Fragments arranged by size:
2162 1375 201 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 1667 1841 2232 2259 3604 1667
Size: 174 391 27 1345 2145
Fragments arranged by size:
2145 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 198 402 745 807 837 961 982 1907
Size: 45 204 343 62 30 124 21 925
Cuts at: 1907 2339 3086 3354 153
Size: 432 747 268 881
Fragments arranged by size:
925 881 747 432 343 268 204 124
62 45 30 21
EcoO109I rG'GnC_Cy
Cuts at: 413 656 657 933 1221 413
Size: 243 1 276 288 3274
Fragments arranged by size:
3274 288 276 243 1
EcoRI G'AATT_C
Cuts at: 1086 1086
Size: 4082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 813 1516 1764 2786 3765 813
Size: 703 248 1022 979 1130
Fragments arranged by size:
1130 1022 979 703 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1924 1954 1956 1986 491
Size: 32 1401 30 2 30 2587
Fragments arranged by size:
2587 1401 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 1512 1760 1512
Size: 248 3834
KpnI G_GTAC'C
Cuts at: 1102 1102
Size: 4082
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1070 2753 2937 1001
Size: 69 1683 184 2146
Fragments arranged by size:
2146 1683 184 69
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 187 568 2071 2198 187
Size: 381 1503 127 2071
Fragments arranged by size:
2071 1503 381 127
MspA1I CmG'CkG
Cuts at: 729 1193 1507 1867 2880 3125 729
Size: 464 314 360 1013 245 1686
Fragments arranged by size:
1686 1013 464 360 314 245
NaeI GCC'GGC
Cuts at: 522 2263 522
Size: 1741 2341
NarI GG'CG_CC
Cuts at: 1513 1761 1513
Size: 248 3834
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 520 2261 520
Size: 1741 2341
NspI r_CATG'y
Cuts at: 708 1135 1635 2542 708
Size: 427 500 907 2248
Fragments arranged by size:
2248 907 500 427
PciI A'CATG_T
Cuts at: 704 2538 704
Size: 1834 2248
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1018 1050 3221 3253 1018
Size: 32 2171 32 1847
Fragments arranged by size:
2171 1847 32 32
PpuMI rG'GwC_Cy
Cuts at: 933 933
Size: 4082
PspOMI G'GGCC_C
Cuts at: 656 656
Size: 4082
PstI C_TGCA'G
Cuts at: 543 1129 1814 543
Size: 586 685 2811
Fragments arranged by size:
2811 685 586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 729 1507 1867 729
Size: 778 360 2944
Fragments arranged by size:
2944 778 360
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SanDI GG'GwC_CC
Cuts at: 933 933
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 2105 2315 2105
Size: 210 3872
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 3599 3599
Size: 4082
SfcI C'TryA_G
Cuts at: 33 539 1125 1810 2803 2994 33
Size: 506 586 685 993 191 1121
Fragments arranged by size:
1121 993 685 586 506 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 412 412
Size: 4082
SfoI GGC'GCC
Cuts at: 1514 1762 1514
Size: 248 3834
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 1104 1104
Size: 4082
SmlI C'TyrA_G
Cuts at: 1237 2152 2644 2906 1237
Size: 915 492 262 2413
Fragments arranged by size:
2413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
SspI AAT'ATT
Cuts at: 394 394
Size: 4082
StuI AGG'CCT
Cuts at: 766 766
Size: 4082
StyI C'CwwG_G
Cuts at: 107 1216 1570 107
Size: 1109 354 2619
Fragments arranged by size:
2619 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 448 1665 1798 2257 2466 448
Size: 1217 133 459 209 2064
Fragments arranged by size:
2064 1217 459 209 133
TatI w'GTAC_w
Cuts at: 572 917 3597 572
Size: 345 2680 1057
Fragments arranged by size:
2680 1057 345
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XmaI C'CCGG_G
Cuts at: 1102 1102
Size: 4082
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI