Restriction map for
pF1KE0503
(Circular) (Six-base) MAPSORT of: pF1KE0503.seq Check: 3406 from: 1 to: 3773
pF1KE0503 3773 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 811 811
Size: 3773
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1598 2030 2777 3045 436
Size: 1162 432 747 268 1164
Fragments arranged by size:
1164 1162 747 432 268
AfeI AGC'GCT
Cuts at: 115 232 115
Size: 117 3656
AflIII A'CryG_T
Cuts at: 1125 2229 3206 1125
Size: 1104 977 1692
Fragments arranged by size:
1692 1104 977
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 743 743
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 180 198 354 393 624 1078 2645 180
Size: 18 156 39 231 454 1567 1308
Fragments arranged by size:
1567 1308 454 231 156 39 18
ApaLI G'TGCA_C
Cuts at: 2543 2543
Size: 3773
ApoI r'AATT_y
Cuts at: 777 3126 3382 777
Size: 2349 256 1168
Fragments arranged by size:
2349 1168 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 793 793
Size: 3773
BamHI G'GATC_C
Cuts at: 494 798 494
Size: 304 3469
BanI G'GyrC_C
Cuts at: 124 148 280 789 1203 1451 1486 124
Size: 24 132 509 414 248 35 2411
Fragments arranged by size:
2411 509 414 248 132 35 24
BanII G_rGCy'C
Cuts at: 787 1817 787
Size: 1030 2743
BbeI G_GCGC'C
Cuts at: 128 1207 1455 128
Size: 1079 248 2446
Fragments arranged by size:
2446 1079 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 270 304 270
Size: 34 3739
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglI GCCn_nnn'nGGC
Cuts at: 421 421
Size: 3773
BglII A'GATC_T
Cuts at: 1288 3325 1288
Size: 2037 1736
BlpI GC'TnA_GC
Cuts at: 250 461 885 250
Size: 211 424 3138
Fragments arranged by size:
3138 424 211
Bme1580I G_kGCm'C
Cuts at: 153 1398 1491 2547 153
Size: 1245 93 1056 1379
Fragments arranged by size:
1379 1245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 729 985 1066 1401 3545 729
Size: 256 81 335 2144 957
Fragments arranged by size:
2144 957 335 256 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 3773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 556 595 331
Size: 225 39 3509
Fragments arranged by size:
3509 225 39
Bpu10I CC'TnA_GC
Cuts at: 382 382
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 603 1126 603
Size: 523 3250
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 125 1204 1452 2154 3650 125
Size: 1079 248 702 1496 248
Fragments arranged by size:
1496 1079 702 248 248
BsaWI w'CCGG_w
Cuts at: 491 545 656 962 1483 2161 2435 2582 2993
Size: 54 111 306 521 678 274 147 411
Cuts at: 2993 491
Size: 1271
Fragments arranged by size:
1271 678 521 411 306 274 147 111
54
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 517 148
Size: 369 3404
BseYI C'CCAG_C
Cuts at: 214 416 680 1198 1956 2533 3029 214
Size: 202 264 518 758 577 496 958
Fragments arranged by size:
958 758 577 518 496 264 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 319
Size: 3773
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 383 491 571 787 1565 1755 2547 383
Size: 108 80 216 778 190 792 1609
Fragments arranged by size:
1609 792 778 216 190 108 80
Bsp1286I G_dGCh'C
Cuts at: 153 383 491 571 787 1398 1491 1565 1755
Size: 230 108 80 216 611 93 74 190
Cuts at: 1755 1817 2547 153
Size: 62 730 1379
Fragments arranged by size:
1379 730 611 230 216 190 108 93
80 74 62
BspEI T'CCGG_A
Cuts at: 656 2993 656
Size: 2337 1436
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 650 823 1037 1339 1720 449
Size: 201 173 214 302 381 2502
Fragments arranged by size:
2502 381 302 214 201 173
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1685 1685
Size: 3773
BsrFI r'CCGG_y
Cuts at: 428 1771 1952 2161 428
Size: 1343 181 209 2040
Fragments arranged by size:
2040 1343 209 181
BsrGI T'GTAC_A
Cuts at: 341 341
Size: 3773
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 378 393 1150 378
Size: 15 757 3001
Fragments arranged by size:
3001 757 15
BstBI TT'CG_AA
Cuts at: 290 2134 290
Size: 1844 1929
BstYI r'GATC_y
Cuts at: 1 314 494 798 1265 1288 1623 1869 2881
Size: 313 180 304 467 23 335 246 1012
Cuts at: 2881 2967 2979 3325 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 313 304 246
180 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3773
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 529 1723 1904 2067 529
Size: 1194 181 163 2235
Fragments arranged by size:
2235 1194 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1016
Size: 3773
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 1479 2337 1479
Size: 858 2915
EaeI y'GGCC_r
Cuts at: 1358 1532 1923 1950 3295 1358
Size: 174 391 27 1345 1836
Fragments arranged by size:
1836 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 1981 2291 2437 3583 157
Size: 1824 310 146 1146 347
Fragments arranged by size:
1824 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 436 556 595 1598 2030 2777 3045 331
Size: 105 120 39 1003 432 747 268 1059
Fragments arranged by size:
1059 1003 747 432 268 120 105 39
EcoNI CCTnn'n_nnAGG
Cuts at: 202 202
Size: 3773
EcoO109I rG'GnC_Cy
Cuts at: 712 912 712
Size: 200 3573
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 117 128 234 271 354 394 1207 1455 2477
Size: 11 106 37 83 40 813 248 1022
Cuts at: 2477 3456 117
Size: 979 434
Fragments arranged by size:
1022 979 813 434 248 106 83 40
37 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1645 1647 1677 1615
Size: 30 2 30 3711
Fragments arranged by size:
3711 30 30 2
HincII GTy'rAC
Cuts at: 812 812
Size: 3773
KasI G'GCGC_C
Cuts at: 124 1203 1451 124
Size: 1079 248 2446
Fragments arranged by size:
2446 1079 248
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2628 2444
Size: 184 3589
MscI TGG'CCA
Cuts at: 1534 1534
Size: 3773
MslI CAynn'nnrTG
Cuts at: 1762 1889 1762
Size: 127 3646
MspA1I CmG'CkG
Cuts at: 177 510 884 1198 1558 2571 2816 177
Size: 333 374 314 360 1013 245 1134
Fragments arranged by size:
1134 1013 374 360 333 314 245
NaeI GCC'GGC
Cuts at: 430 1954 430
Size: 1524 2249
NarI GG'CG_CC
Cuts at: 125 1204 1452 125
Size: 1079 248 2446
Fragments arranged by size:
2446 1079 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 428 1952 428
Size: 1524 2249
NspI r_CATG'y
Cuts at: 826 1326 2233 826
Size: 500 907 2366
Fragments arranged by size:
2366 907 500
PasI CC'CwG_GG
Cuts at: 699 699
Size: 3773
PciI A'CATG_T
Cuts at: 2229 2229
Size: 3773
PfoI T'CCnGG_A
Cuts at: 533 533
Size: 3773
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PpuMI rG'GwC_Cy
Cuts at: 712 712
Size: 3773
PstI C_TGCA'G
Cuts at: 635 668 820 1505 635
Size: 33 152 685 2903
Fragments arranged by size:
2903 685 152 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 177 1198 1558 177
Size: 1021 360 2392
Fragments arranged by size:
2392 1021 360
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 361 631 664 816 1501 2494 2685 33
Size: 328 270 33 152 685 993 191 1121
Fragments arranged by size:
1121 993 685 328 270 191 152 33
SfoI GGC'GCC
Cuts at: 126 1205 1453 126
Size: 1079 248 2446
Fragments arranged by size:
2446 1079 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 928 1843 2335 2597 928
Size: 915 492 262 2104
Fragments arranged by size:
2104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StuI AGG'CCT
Cuts at: 424 424
Size: 3773
StyI C'CwwG_G
Cuts at: 107 128 907 1261 107
Size: 21 779 354 2619
Fragments arranged by size:
2619 779 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1356 1489 1948 2157 1356
Size: 133 459 209 2972
Fragments arranged by size:
2972 459 209 133
TatI w'GTAC_w
Cuts at: 341 3288 341
Size: 2947 826
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 3741
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1268 1268
Size: 3773
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI