(Circular) (Six-base) MAPSORT of: pF1KE0503.seq Check: 3406 from: 1 to: 3773 pF1KE0503 3773 bp With 182 enzymes: SgfI * April 24, 2009 13:15 .. AccI GT'mk_AC Cuts at: 811 811 Size: 3773 Acc65I G'GTAC_C Cuts at: 789 789 Size: 3773 AclI AA'CG_TT Cuts at: 3613 3613 Size: 3773 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 436 1598 2030 2777 3045 436 Size: 1162 432 747 268 1164 Fragments arranged by size: 1164 1162 747 432 268 AfeI AGC'GCT Cuts at: 115 232 115 Size: 117 3656 AflIII A'CryG_T Cuts at: 1125 2229 3206 1125 Size: 1104 977 1692 Fragments arranged by size: 1692 1104 977 AgeI A'CCGG_T Cuts at: 2161 2161 Size: 3773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 743 743 Size: 3773 AlwNI CAG_nnn'CTG Cuts at: 180 198 354 393 624 1078 2645 180 Size: 18 156 39 231 454 1567 1308 Fragments arranged by size: 1567 1308 454 231 156 39 18 ApaLI G'TGCA_C Cuts at: 2543 2543 Size: 3773 ApoI r'AATT_y Cuts at: 777 3126 3382 777 Size: 2349 256 1168 Fragments arranged by size: 2349 1168 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3773 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3773 AvaI C'yCGr_G Cuts at: 793 793 Size: 3773 BamHI G'GATC_C Cuts at: 494 798 494 Size: 304 3469 BanI G'GyrC_C Cuts at: 124 148 280 789 1203 1451 1486 124 Size: 24 132 509 414 248 35 2411 Fragments arranged by size: 2411 509 414 248 132 35 24 BanII G_rGCy'C Cuts at: 787 1817 787 Size: 1030 2743 BbeI G_GCGC'C Cuts at: 128 1207 1455 128 Size: 1079 248 2446 Fragments arranged by size: 2446 1079 248 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 270 304 270 Size: 34 3739 BciVI GTATCCnnnnn_n' Cuts at: 1671 2432 3773 1671 Size: 761 1341 1671 Fragments arranged by size: 1671 1341 761 BclI T'GATC_A Cuts at: 1293 1293 Size: 3773 BglI GCCn_nnn'nGGC Cuts at: 421 421 Size: 3773 BglII A'GATC_T Cuts at: 1288 3325 1288 Size: 2037 1736 BlpI GC'TnA_GC Cuts at: 250 461 885 250 Size: 211 424 3138 Fragments arranged by size: 3138 424 211 Bme1580I G_kGCm'C Cuts at: 153 1398 1491 2547 153 Size: 1245 93 1056 1379 Fragments arranged by size: 1379 1245 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 729 985 1066 1401 3545 729 Size: 256 81 335 2144 957 Fragments arranged by size: 2144 957 335 256 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 3773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 331 556 595 331 Size: 225 39 3509 Fragments arranged by size: 3509 225 39 Bpu10I CC'TnA_GC Cuts at: 382 382 Size: 3773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 949 1828 2320 2618 949 Size: 879 492 298 2104 Fragments arranged by size: 2104 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3477 3477 Size: 3773 BsaAI yAC'GTr Cuts at: 603 1126 603 Size: 523 3250 BsaBI GATnn'nnATC Cuts at: 6 1287 3324 6 Size: 1281 2037 455 Fragments arranged by size: 2037 1281 455 BsaHI Gr'CG_yC Cuts at: 125 1204 1452 2154 3650 125 Size: 1079 248 702 1496 248 Fragments arranged by size: 1496 1079 702 248 248 BsaWI w'CCGG_w Cuts at: 491 545 656 962 1483 2161 2435 2582 2993 Size: 54 111 306 521 678 274 147 411 Cuts at: 2993 491 Size: 1271 Fragments arranged by size: 1271 678 521 411 306 274 147 111 54 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 148 517 148 Size: 369 3404 BseYI C'CCAG_C Cuts at: 214 416 680 1198 1956 2533 3029 214 Size: 202 264 518 758 577 496 958 Fragments arranged by size: 958 758 577 518 496 264 202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 319 319 Size: 3773 BsiEI CG_ry'CG Cuts at: 104 1315 1361 2569 104 Size: 1211 46 1208 1308 Fragments arranged by size: 1308 1211 1208 46 BsiHKAI G_wGCw'C Cuts at: 383 491 571 787 1565 1755 2547 383 Size: 108 80 216 778 190 792 1609 Fragments arranged by size: 1609 792 778 216 190 108 80 Bsp1286I G_dGCh'C Cuts at: 153 383 491 571 787 1398 1491 1565 1755 Size: 230 108 80 216 611 93 74 190 Cuts at: 1755 1817 2547 153 Size: 62 730 1379 Fragments arranged by size: 1379 730 611 230 216 190 108 93 80 74 62 BspEI T'CCGG_A Cuts at: 656 2993 656 Size: 2337 1436 BspHI T'CATG_A Cuts at: 2949 2949 Size: 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 449 650 823 1037 1339 1720 449 Size: 201 173 214 302 381 2502 Fragments arranged by size: 2502 381 302 214 201 173 BsrBI CCG'CTC Cuts at: 50 2065 2119 3603 3768 50 Size: 2015 54 1484 165 55 Fragments arranged by size: 2015 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1685 1685 Size: 3773 BsrFI r'CCGG_y Cuts at: 428 1771 1952 2161 428 Size: 1343 181 209 2040 Fragments arranged by size: 2040 1343 209 181 BsrGI T'GTAC_A Cuts at: 341 341 Size: 3773 BssSI C'ACGA_G Cuts at: 2044 2402 2044 Size: 358 3415 BstAPI GCAn_nnn'nTGC Cuts at: 378 393 1150 378 Size: 15 757 3001 Fragments arranged by size: 3001 757 15 BstBI TT'CG_AA Cuts at: 290 2134 290 Size: 1844 1929 BstYI r'GATC_y Cuts at: 1 314 494 798 1265 1288 1623 1869 2881 Size: 313 180 304 467 23 335 246 1012 Cuts at: 2881 2967 2979 3325 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 346 335 313 304 246 180 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3773 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 529 1723 1904 2067 529 Size: 1194 181 163 2235 Fragments arranged by size: 2235 1194 181 163 BtsI GCAGTG_nn' Cuts at: 1016 1016 Size: 3773 DraI TTT'AAA Cuts at: 772 772 Size: 3773 DrdI GACnn_nn'nnGTC Cuts at: 1479 2337 1479 Size: 858 2915 EaeI y'GGCC_r Cuts at: 1358 1532 1923 1950 3295 1358 Size: 174 391 27 1345 1836 Fragments arranged by size: 1836 1345 391 174 27 EagI C'GGCC_G Cuts at: 1358 1358 Size: 3773 EarI CTCTTCn'nnn_ Cuts at: 1796 2006 1796 Size: 210 3563 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 157 1981 2291 2437 3583 157 Size: 1824 310 146 1146 347 Fragments arranged by size: 1824 1146 347 310 146 EcoICRI GAG'CTC Cuts at: 785 785 Size: 3773 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 331 436 556 595 1598 2030 2777 3045 331 Size: 105 120 39 1003 432 747 268 1059 Fragments arranged by size: 1059 1003 747 432 268 120 105 39 EcoNI CCTnn'n_nnAGG Cuts at: 202 202 Size: 3773 EcoO109I rG'GnC_Cy Cuts at: 712 912 712 Size: 200 3573 EcoRI G'AATT_C Cuts at: 777 777 Size: 3773 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1792 1792 Size: 3773 FspI TGC'GCA Cuts at: 1554 1554 Size: 3773 HaeII r_GCGC'y Cuts at: 117 128 234 271 354 394 1207 1455 2477 Size: 11 106 37 83 40 813 248 1022 Cuts at: 2477 3456 117 Size: 979 434 Fragments arranged by size: 1022 979 813 434 248 106 83 40 37 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1615 1645 1647 1677 1615 Size: 30 2 30 3711 Fragments arranged by size: 3711 30 30 2 HincII GTy'rAC Cuts at: 812 812 Size: 3773 KasI G'GCGC_C Cuts at: 124 1203 1451 124 Size: 1079 248 2446 Fragments arranged by size: 2446 1079 248 KpnI G_GTAC'C Cuts at: 793 793 Size: 3773 MluI A'CGCG_T Cuts at: 3206 3206 Size: 3773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2444 2628 2444 Size: 184 3589 MscI TGG'CCA Cuts at: 1534 1534 Size: 3773 MslI CAynn'nnrTG Cuts at: 1762 1889 1762 Size: 127 3646 MspA1I CmG'CkG Cuts at: 177 510 884 1198 1558 2571 2816 177 Size: 333 374 314 360 1013 245 1134 Fragments arranged by size: 1134 1013 374 360 333 314 245 NaeI GCC'GGC Cuts at: 430 1954 430 Size: 1524 2249 NarI GG'CG_CC Cuts at: 125 1204 1452 125 Size: 1079 248 2446 Fragments arranged by size: 2446 1079 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3773 NgoMIV G'CCGG_C Cuts at: 428 1952 428 Size: 1524 2249 NspI r_CATG'y Cuts at: 826 1326 2233 826 Size: 500 907 2366 Fragments arranged by size: 2366 907 500 PasI CC'CwG_GG Cuts at: 699 699 Size: 3773 PciI A'CATG_T Cuts at: 2229 2229 Size: 3773 PfoI T'CCnGG_A Cuts at: 533 533 Size: 3773 PmeI GTTT'AAAC Cuts at: 772 772 Size: 3773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2912 2944 2912 Size: 32 3741 PpuMI rG'GwC_Cy Cuts at: 712 712 Size: 3773 PstI C_TGCA'G Cuts at: 635 668 820 1505 635 Size: 33 152 685 2903 Fragments arranged by size: 2903 685 152 33 PvuI CG_AT'CG Cuts at: 104 104 Size: 3773 PvuII CAG'CTG Cuts at: 177 1198 1558 177 Size: 1021 360 2392 Fragments arranged by size: 2392 1021 360 RsrII CG'GwC_CG Cuts at: 1968 1968 Size: 3773 SacI G_AGCT'C Cuts at: 787 787 Size: 3773 SalI G'TCGA_C Cuts at: 810 810 Size: 3773 SapI GCTCTTCn'nnn_ Cuts at: 1796 2006 1796 Size: 210 3563 SbfI CC_TGCA'GG Cuts at: 820 820 Size: 3773 ScaI AGT'ACT Cuts at: 3290 3290 Size: 3773 SfcI C'TryA_G Cuts at: 33 361 631 664 816 1501 2494 2685 33 Size: 328 270 33 152 685 993 191 1121 Fragments arranged by size: 1121 993 685 328 270 191 152 33 SfoI GGC'GCC Cuts at: 126 1205 1453 126 Size: 1079 248 2446 Fragments arranged by size: 2446 1079 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3773 SmaI CCC'GGG Cuts at: 795 795 Size: 3773 SmlI C'TyrA_G Cuts at: 928 1843 2335 2597 928 Size: 915 492 262 2104 Fragments arranged by size: 2104 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3773 SphI G_CATG'C Cuts at: 826 1326 826 Size: 500 3273 StuI AGG'CCT Cuts at: 424 424 Size: 3773 StyI C'CwwG_G Cuts at: 107 128 907 1261 107 Size: 21 779 354 2619 Fragments arranged by size: 2619 779 354 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1356 1489 1948 2157 1356 Size: 133 459 209 2972 Fragments arranged by size: 2972 459 209 133 TatI w'GTAC_w Cuts at: 341 3288 341 Size: 2947 826 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 3290 Size: 32 3741 Tth111I GACn'n_nGTC Cuts at: 1570 1570 Size: 3773 XbaI T'CTAG_A Cuts at: 804 804 Size: 3773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1268 1268 Size: 3773 XmaI C'CCGG_G Cuts at: 793 793 Size: 3773 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI