Restriction map for
pF1KE0505
(Circular) (Six-base) MAPSORT of: pF1KE0505.seq Check: 3129 from: 1 to: 3866
pF1KE0505 3866 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 516 516
Size: 3866
AccI GT'mk_AC
Cuts at: 904 904
Size: 3866
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 443 536 1691 2123 2870 3138 346
Size: 97 93 1155 432 747 268 1074
Fragments arranged by size:
1155 1074 747 432 268 97 93
AfeI AGC'GCT
Cuts at: 361 361
Size: 3866
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AhdI GACnn_n'nnGTC
Cuts at: 707 707
Size: 3866
AleI CACnn'nnGTG
Cuts at: 449 542 449
Size: 93 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 455 548 455
Size: 93 3773
AlwNI CAG_nnn'CTG
Cuts at: 1171 2738 1171
Size: 1567 2299
ApaI G_GGCC'C
Cuts at: 139 139
Size: 3866
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 870 3219 3475 870
Size: 2349 256 1261
Fragments arranged by size:
2349 1261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 886 886
Size: 3866
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 882 1296 1544 1579 882
Size: 414 248 35 3169
Fragments arranged by size:
3169 414 248 35
BanII G_rGCy'C
Cuts at: 139 880 1910 139
Size: 741 1030 2095
Fragments arranged by size:
2095 1030 741
BbeI G_GCGC'C
Cuts at: 1300 1548 1300
Size: 248 3618
BbsI GAAGACnn'nnnn_
Cuts at: 316 650 698 746 316
Size: 334 48 48 3436
Fragments arranged by size:
3436 334 48 48
BbvCI CC'TCA_GC
Cuts at: 298 298
Size: 3866
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 1386 1386
Size: 3866
BglII A'GATC_T
Cuts at: 612 1381 3418 612
Size: 769 2037 1060
Fragments arranged by size:
2037 1060 769
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 139 1491 1584 2640 139
Size: 1352 93 1056 1365
Fragments arranged by size:
1365 1352 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1078 1159 1494 3638 1078
Size: 81 335 2144 1306
Fragments arranged by size:
2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 3866
Bpu10I CC'TnA_GC
Cuts at: 298 298
Size: 3866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1921 2413 2711 1042
Size: 879 492 298 2197
Fragments arranged by size:
2197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 715 815 3570 715
Size: 100 2755 1011
Fragments arranged by size:
2755 1011 100
BsaAI yAC'GTr
Cuts at: 1219 1219
Size: 3866
BsaBI GATnn'nnATC
Cuts at: 6 1380 3417 6
Size: 1374 2037 455
Fragments arranged by size:
2037 1374 455
BsaHI Gr'CG_yC
Cuts at: 313 347 436 1297 1545 2247 3743 313
Size: 34 89 861 248 702 1496 436
Fragments arranged by size:
1496 861 702 436 248 89 34
BsaWI w'CCGG_w
Cuts at: 753 1055 1576 2254 2528 2675 3086 753
Size: 302 521 678 274 147 411 1533
Fragments arranged by size:
1533 678 521 411 302 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 337 367 337
Size: 30 3836
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 565 472
Size: 93 3773
BseYI C'CCAG_C
Cuts at: 139 791 1291 2049 2626 3122 139
Size: 652 500 758 577 496 883
Fragments arranged by size:
883 758 652 577 500 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 451 518 544 425
Size: 26 67 26 3747
Fragments arranged by size:
3747 67 26 26
BsiEI CG_ry'CG
Cuts at: 104 1408 1454 2662 104
Size: 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 46
BsiHKAI G_wGCw'C
Cuts at: 880 1658 1848 2640 880
Size: 778 190 792 2106
Fragments arranged by size:
2106 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 375 375
Size: 3866
Bsp1286I G_dGCh'C
Cuts at: 139 880 1491 1584 1658 1848 1910 2640 139
Size: 741 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 741 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3086 3086
Size: 3866
BspHI T'CATG_A
Cuts at: 650 698 746 3042 650
Size: 48 48 2296 1474
Fragments arranged by size:
2296 1474 48 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 916 1130 1432 1813 516
Size: 400 214 302 381 2569
Fragments arranged by size:
2569 400 381 302 214
BsrBI CCG'CTC
Cuts at: 50 353 2158 2212 3696 3861 50
Size: 303 1805 54 1484 165 55
Fragments arranged by size:
1805 1484 303 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1778
Size: 3866
BsrFI r'CCGG_y
Cuts at: 1864 2045 2254 1864
Size: 181 209 3476
Fragments arranged by size:
3476 209 181
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 250 1243 250
Size: 993 2873
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstXI CCAn_nnnn'nTGG
Cuts at: 342 342
Size: 3866
BstYI r'GATC_y
Cuts at: 1 612 660 891 1358 1381 1716 1962 2974
Size: 611 48 231 467 23 335 246 1012
Cuts at: 2974 3060 3072 3418 1
Size: 86 12 346 449
Fragments arranged by size:
1012 611 467 449 346 335 246 231
86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 488 488
Size: 3866
BtgI C'CryG_G
Cuts at: 107 107
Size: 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 239 1109 239
Size: 870 2996
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 1451 1625 2016 2043 3388 1451
Size: 174 391 27 1345 1929
Fragments arranged by size:
1929 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 878 878
Size: 3866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 443 536 1691 2123 2870 3138 346
Size: 97 93 1155 432 747 268 1074
Fragments arranged by size:
1155 1074 747 432 268 97 93
EcoO109I rG'GnC_Cy
Cuts at: 136 147 181 415 1005 136
Size: 11 34 234 590 2997
Fragments arranged by size:
2997 590 234 34 11
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3866
FspI TGC'GCA
Cuts at: 1647 1647
Size: 3866
HaeII r_GCGC'y
Cuts at: 363 1300 1548 2570 3549 363
Size: 937 248 1022 979 680
Fragments arranged by size:
1022 979 937 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 384 416 645 677 693 725 1708
Size: 32 15 32 229 32 16 32 983
Cuts at: 1708 1738 1740 1770 337
Size: 30 2 30 2433
Fragments arranged by size:
2433 983 229 32 32 32 32 30
30 16 15 2
HincII GTy'rAC
Cuts at: 905 905
Size: 3866
KasI G'GCGC_C
Cuts at: 1296 1544 1296
Size: 248 3618
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2721 2537
Size: 184 3682
MscI TGG'CCA
Cuts at: 1627 1627
Size: 3866
MslI CAynn'nnrTG
Cuts at: 194 449 542 1855 1982 194
Size: 255 93 1313 127 2078
Fragments arranged by size:
2078 1313 255 127 93
MspA1I CmG'CkG
Cuts at: 143 977 1291 1651 2664 2909 143
Size: 834 314 360 1013 245 1100
Fragments arranged by size:
1100 1013 834 360 314 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 1297 1545 1297
Size: 248 3618
NcoI C'CATG_G
Cuts at: 107 107
Size: 3866
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PflMI CCAn_nnn'nTGG
Cuts at: 362 603 362
Size: 241 3625
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3834
PpuMI rG'GwC_Cy
Cuts at: 181 415 181
Size: 234 3632
PshAI GACnn'nnGTC
Cuts at: 506 506
Size: 3866
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 3866
PstI C_TGCA'G
Cuts at: 169 913 1598 169
Size: 744 685 2437
Fragments arranged by size:
2437 744 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 1291 1651 1291
Size: 360 3506
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 880 880
Size: 3866
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SanDI GG'GwC_CC
Cuts at: 181 181
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
SbfI CC_TGCA'GG
Cuts at: 913 913
Size: 3866
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 165 909 1594 2587 2778 33
Size: 132 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 191 132
SfoI GGC'GCC
Cuts at: 1298 1546 1298
Size: 248 3618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 888 888
Size: 3866
SmlI C'TyrA_G
Cuts at: 1021 1936 2428 2690 1021
Size: 915 492 262 2197
Fragments arranged by size:
2197 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3866
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
StyI C'CwwG_G
Cuts at: 107 1000 1354 107
Size: 893 354 2619
Fragments arranged by size:
2619 893 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1449 1582 2041 2250 1449
Size: 133 459 209 3065
Fragments arranged by size:
3065 459 209 133
TatI w'GTAC_w
Cuts at: 3381 3381
Size: 3866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 3834
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 402 897 402
Size: 495 3371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1361 1361
Size: 3866
XmaI C'CCGG_G
Cuts at: 886 886
Size: 3866
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BcgI BfrBI
BglI BmgBI BmtI BpmI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI