Restriction map for
pF1KE0506
(Circular) (Six-base) MAPSORT of: pF1KE0506.seq Check: 2358 from: 1 to: 4100
pF1KE0506 4100 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 1925 2357 3104 3372 216
Size: 1709 432 747 268 944
Fragments arranged by size:
1709 944 747 432 268
AflIII A'CryG_T
Cuts at: 347 434 1452 2556 3533 347
Size: 87 1018 1104 977 914
Fragments arranged by size:
1104 1018 977 914 87
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AleI CACnn'nnGTG
Cuts at: 362 362
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 495 924 1405 2972 495
Size: 429 481 1567 1623
Fragments arranged by size:
1623 1567 481 429
ApaI G_GGCC'C
Cuts at: 617 617
Size: 4100
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 1104 3453 3709 1104
Size: 2349 256 1495
Fragments arranged by size:
2349 1495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 882 1033 1120 882
Size: 151 87 3862
Fragments arranged by size:
3862 151 87
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 1029 1116 1530 1778 1813 1029
Size: 87 414 248 35 3316
Fragments arranged by size:
3316 414 248 87 35
BanII G_rGCy'C
Cuts at: 617 646 1114 2144 617
Size: 29 468 1030 2573
Fragments arranged by size:
2573 1030 468 29
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BbvCI CC'TCA_GC
Cuts at: 323 323
Size: 4100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1038 1072 1038
Size: 34 4066
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 145 1212 145
Size: 1067 3033
Bme1580I G_kGCm'C
Cuts at: 617 1034 1725 1818 2874 617
Size: 417 691 93 1056 1843
Fragments arranged by size:
1843 1056 691 417 93
BmrI ACTGGGnnnn_n'
Cuts at: 851 1312 1393 1728 3872 851
Size: 461 81 335 2144 1079
Fragments arranged by size:
2144 1079 461 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 771 2467 771
Size: 1696 2404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1051
Size: 4100
Bpu10I CC'TnA_GC
Cuts at: 323 323
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 169 238 3804 169
Size: 69 3566 465
Fragments arranged by size:
3566 465 69
BsaAI yAC'GTr
Cuts at: 435 1453 435
Size: 1018 3082
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1289 1810 2488 2762 2909 3320 1289
Size: 521 678 274 147 411 2069
Fragments arranged by size:
2069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 545 575 545
Size: 30 4070
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 870 292
Size: 578 3522
BseYI C'CCAG_C
Cuts at: 382 616 772 1525 2283 2860 3356 382
Size: 234 156 753 758 577 496 1126
Fragments arranged by size:
1126 758 753 577 496 234 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 100 394 100
Size: 294 3806
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 1114 1892 2082 2874 431
Size: 683 778 190 792 1657
Fragments arranged by size:
1657 792 778 683 190
BsmBI CGTCTCn'nnnn_
Cuts at: 359 676 359
Size: 317 3783
Bsp1286I G_dGCh'C
Cuts at: 431 617 646 1034 1114 1725 1818 1892 2082
Size: 186 29 388 80 611 93 74 190
Cuts at: 2082 2144 2874 431
Size: 62 730 1657
Fragments arranged by size:
1657 730 611 388 190 186 93 80
74 62 29
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 952 1150 1364 1666 2047 952
Size: 198 214 302 381 3005
Fragments arranged by size:
3005 381 302 214 198
BsrBI CCG'CTC
Cuts at: 50 186 2392 2446 3930 4095 50
Size: 136 2206 54 1484 165 55
Fragments arranged by size:
2206 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 594 2012 594
Size: 1418 2682
BsrFI r'CCGG_y
Cuts at: 218 2098 2279 2488 218
Size: 1880 181 209 1830
Fragments arranged by size:
1880 1830 209 181
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 221 958 1477 221
Size: 737 519 2844
Fragments arranged by size:
2844 737 519
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstEII G'GTnAC_C
Cuts at: 387 387
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 336 828 336
Size: 492 3608
BstYI r'GATC_y
Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306
Size: 1124 467 23 335 246 1012 86 12
Cuts at: 3306 3652 1
Size: 346 449
Fragments arranged by size:
1124 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 251 251
Size: 4100
BtgI C'CryG_G
Cuts at: 107 500 623 107
Size: 393 123 3584
Fragments arranged by size:
3584 393 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 396 681 2050 2231 2394 396
Size: 285 1369 181 163 2102
Fragments arranged by size:
2102 1369 285 181 163
BtsI GCAGTG_nn'
Cuts at: 377 1343 377
Size: 966 3134
DraI TTT'AAA
Cuts at: 288 1099 288
Size: 811 3289
DraIII CAC_nnn'GTG
Cuts at: 135 435 135
Size: 300 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 422 1685 1859 2250 2277 3622 422
Size: 1263 174 391 27 1345 900
Fragments arranged by size:
1345 1263 900 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 205 727 2123 2333 205
Size: 522 1396 210 1972
Fragments arranged by size:
1972 1396 522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 566 2308 2618 2764 3910 566
Size: 1742 310 146 1146 756
Fragments arranged by size:
1742 1146 756 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 1051 1925 2357 3104 3372 216
Size: 835 874 432 747 268 944
Fragments arranged by size:
944 874 835 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 805 805
Size: 4100
EcoO109I rG'GnC_Cy
Cuts at: 492 964 1062 1239 492
Size: 472 98 177 3353
Fragments arranged by size:
3353 472 177 98
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 216 2119 216
Size: 1903 2197
FspI TGC'GCA
Cuts at: 907 1881 907
Size: 974 3126
FspAI rTGC'GCAy
Cuts at: 907 907
Size: 4100
HaeII r_GCGC'y
Cuts at: 1534 1782 2804 3783 1534
Size: 248 1022 979 1851
Fragments arranged by size:
1851 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 948 980 1942 1972 1974 2004 948
Size: 32 962 30 2 30 3044
Fragments arranged by size:
3044 962 32 30 30 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2771 2955 2771
Size: 184 3916
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 265 362 478 826 2089 2216 265
Size: 97 116 348 1263 127 2149
Fragments arranged by size:
2149 1263 348 127 116 97
MspA1I CmG'CkG
Cuts at: 362 979 1071 1211 1525 1885 2898 3143 362
Size: 617 92 140 314 360 1013 245 1319
Fragments arranged by size:
1319 1013 617 360 314 245 140 92
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NcoI C'CATG_G
Cuts at: 107 107
Size: 4100
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NspI r_CATG'y
Cuts at: 1153 1653 2560 1153
Size: 500 907 2693
Fragments arranged by size:
2693 907 500
PasI CC'CwG_GG
Cuts at: 924 924
Size: 4100
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PflMI CCAn_nnn'nTGG
Cuts at: 516 1026 516
Size: 510 3590
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PmlI CAC'GTG
Cuts at: 435 435
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PpuMI rG'GwC_Cy
Cuts at: 492 964 1062 492
Size: 472 98 3530
Fragments arranged by size:
3530 472 98
PspOMI G'GGCC_C
Cuts at: 613 613
Size: 4100
PstI C_TGCA'G
Cuts at: 1147 1832 1147
Size: 685 3415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 1525 1885 1525
Size: 360 3740
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 205 2123 2333 205
Size: 1918 210 1972
Fragments arranged by size:
1972 1918 210
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 1143 1828 2821 3012 33
Size: 1110 685 993 191 1121
Fragments arranged by size:
1121 1110 993 685 191
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 1122 1122
Size: 4100
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
StuI AGG'CCT
Cuts at: 1085 1085
Size: 4100
StyI C'CwwG_G
Cuts at: 107 743 1234 1588 107
Size: 636 491 354 2619
Fragments arranged by size:
2619 636 491 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 396 1683 1816 2275 2484 193
Size: 203 1287 133 459 209 1809
Fragments arranged by size:
1809 1287 459 209 203 133
TatI w'GTAC_w
Cuts at: 3615 3615
Size: 4100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 615 647 3617 3649 545
Size: 32 38 32 2970 32 996
Fragments arranged by size:
2970 996 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1595 1595
Size: 4100
XmaI C'CCGG_G
Cuts at: 1120 1120
Size: 4100
XmnI GAAnn'nnTTC
Cuts at: 655 850 655
Size: 195 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI