Restriction map for
pF1KE0507
(Circular) (Six-base) MAPSORT of: pF1KE0507.seq Check: 6334 from: 1 to: 3890
pF1KE0507 3890 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 413 413
Size: 3890
AccI GT'mk_AC
Cuts at: 928 928
Size: 3890
Acc65I G'GTAC_C
Cuts at: 906 906
Size: 3890
AclI AA'CG_TT
Cuts at: 3730 3730
Size: 3890
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1715 2147 2894 3162 1715
Size: 432 747 268 2443
Fragments arranged by size:
2443 747 432 268
AflIII A'CryG_T
Cuts at: 1242 2346 3323 1242
Size: 1104 977 1809
Fragments arranged by size:
1809 1104 977
AgeI A'CCGG_T
Cuts at: 2278 2278
Size: 3890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 737 737
Size: 3890
AlwNI CAG_nnn'CTG
Cuts at: 531 575 1195 2762 531
Size: 44 620 1567 1659
Fragments arranged by size:
1659 1567 620 44
ApaI G_GGCC'C
Cuts at: 196 294 721 846 196
Size: 98 427 125 3240
Fragments arranged by size:
3240 427 125 98
ApaLI G'TGCA_C
Cuts at: 2660 2660
Size: 3890
ApoI r'AATT_y
Cuts at: 894 3243 3499 894
Size: 2349 256 1285
Fragments arranged by size:
2349 1285 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3890
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
AvaI C'yCGr_G
Cuts at: 352 458 910 352
Size: 106 452 3332
Fragments arranged by size:
3332 452 106
AvrII C'CTAG_G
Cuts at: 735 735
Size: 3890
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 481 514 481
Size: 33 3857
BamHI G'GATC_C
Cuts at: 915 915
Size: 3890
BanI G'GyrC_C
Cuts at: 906 1320 1568 1603 906
Size: 414 248 35 3193
Fragments arranged by size:
3193 414 248 35
BanII G_rGCy'C
Cuts at: 196 294 721 754 846 904 1934 196
Size: 98 427 33 92 58 1030 2152
Fragments arranged by size:
2152 1030 427 98 92 58 33
BbeI G_GCGC'C
Cuts at: 1324 1572 1324
Size: 248 3642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 377 343
Size: 34 3856
BciVI GTATCCnnnnn_n'
Cuts at: 1788 2549 3890 1788
Size: 761 1341 1788
Fragments arranged by size:
1788 1341 761
BclI T'GATC_A
Cuts at: 1410 1410
Size: 3890
BglI GCCn_nnn'nGGC
Cuts at: 436 653 436
Size: 217 3673
BglII A'GATC_T
Cuts at: 1405 3442 1405
Size: 2037 1853
BlpI GC'TnA_GC
Cuts at: 1002 1002
Size: 3890
Bme1580I G_kGCm'C
Cuts at: 196 294 353 721 846 1515 1608 2664 196
Size: 98 59 368 125 669 93 1056 1422
Fragments arranged by size:
1422 1056 669 368 125 98 93 59
BmrI ACTGGGnnnn_n'
Cuts at: 209 1102 1183 1518 3662 209
Size: 893 81 335 2144 437
Fragments arranged by size:
2144 893 437 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 3890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 634 410
Size: 224 3666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1066 1945 2437 2735 232
Size: 834 879 492 298 1387
Fragments arranged by size:
1387 879 834 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 3594 760
Size: 2834 1056
BsaAI yAC'GTr
Cuts at: 1243 1243
Size: 3890
BsaBI GATnn'nnATC
Cuts at: 6 1404 3441 6
Size: 1398 2037 455
Fragments arranged by size:
2037 1398 455
BsaHI Gr'CG_yC
Cuts at: 1321 1569 2271 3767 1321
Size: 248 702 1496 1444
Fragments arranged by size:
1496 1444 702 248
BsaWI w'CCGG_w
Cuts at: 1079 1600 2278 2552 2699 3110 1079
Size: 521 678 274 147 411 1859
Fragments arranged by size:
1859 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 493 523 616 646 493
Size: 30 93 30 3737
Fragments arranged by size:
3737 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 370 721 138
Size: 232 351 3307
Fragments arranged by size:
3307 351 232
BseYI C'CCAG_C
Cuts at: 156 1315 2073 2650 3146 156
Size: 1159 758 577 496 900
Fragments arranged by size:
1159 900 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 306
Size: 3890
BsiEI CG_ry'CG
Cuts at: 104 1432 1478 2686 104
Size: 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 904 1682 1872 2664 904
Size: 778 190 792 2130
Fragments arranged by size:
2130 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 196 294 353 721 754 846 904 1515 1608
Size: 98 59 368 33 92 58 611 93
Cuts at: 1608 1682 1872 1934 2664 196
Size: 74 190 62 730 1422
Fragments arranged by size:
1422 730 611 368 190 98 93 92
74 62 59 58 33
BspEI T'CCGG_A
Cuts at: 3110 3110
Size: 3890
BspHI T'CATG_A
Cuts at: 3066 3066
Size: 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 413 668 940 1154 1456 1837 266
Size: 147 255 272 214 302 381 2319
Fragments arranged by size:
2319 381 302 272 255 214 147
BsrBI CCG'CTC
Cuts at: 50 548 2182 2236 3720 3885 50
Size: 498 1634 54 1484 165 55
Fragments arranged by size:
1634 1484 498 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1802 1802
Size: 3890
BsrFI r'CCGG_y
Cuts at: 423 1888 2069 2278 423
Size: 1465 181 209 2035
Fragments arranged by size:
2035 1465 209 181
BssSI C'ACGA_G
Cuts at: 2161 2519 2161
Size: 358 3532
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 1267
Size: 3890
BstBI TT'CG_AA
Cuts at: 2251 2251
Size: 3890
BstYI r'GATC_y
Cuts at: 1 915 1382 1405 1740 1986 2998 3084 3096
Size: 914 467 23 335 246 1012 86 12
Cuts at: 3096 3442 1
Size: 346 449
Fragments arranged by size:
1012 914 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2021 2184 1840
Size: 181 163 3546
Fragments arranged by size:
3546 181 163
BtsI GCAGTG_nn'
Cuts at: 624 832 1133 624
Size: 208 301 3381
Fragments arranged by size:
3381 301 208
DraI TTT'AAA
Cuts at: 889 889
Size: 3890
DrdI GACnn_nn'nnGTC
Cuts at: 1596 2454 1596
Size: 858 3032
EaeI y'GGCC_r
Cuts at: 225 335 621 801 1475 1649 2040 2067 3412
Size: 110 286 180 674 174 391 27 1345
Cuts at: 3412 225
Size: 703
Fragments arranged by size:
1345 703 674 391 286 180 174 110
27
EagI C'GGCC_G
Cuts at: 1475 1475
Size: 3890
EarI CTCTTCn'nnn_
Cuts at: 832 1913 2123 832
Size: 1081 210 2599
Fragments arranged by size:
2599 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2098 2408 2554 3700 2098
Size: 310 146 1146 2288
Fragments arranged by size:
2288 1146 310 146
EcoICRI GAG'CTC
Cuts at: 902 902
Size: 3890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 634 1715 2147 2894 3162 410
Size: 224 1081 432 747 268 1138
Fragments arranged by size:
1138 1081 747 432 268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 634 739 634
Size: 105 3785
EcoO109I rG'GnC_Cy
Cuts at: 192 200 291 717 718 842 1029 192
Size: 8 91 426 1 124 187 3053
Fragments arranged by size:
3053 426 187 124 91 8 1
EcoRI G'AATT_C
Cuts at: 894 894
Size: 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3890
FspI TGC'GCA
Cuts at: 181 1671 181
Size: 1490 2400
HaeII r_GCGC'y
Cuts at: 1324 1572 2594 3573 1324
Size: 248 1022 979 1641
Fragments arranged by size:
1641 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1732 1762 1764 1794 1732
Size: 30 2 30 3828
Fragments arranged by size:
3828 30 30 2
HincII GTy'rAC
Cuts at: 929 929
Size: 3890
HindIII A'AGCT_T
Cuts at: 465 465
Size: 3890
KasI G'GCGC_C
Cuts at: 1320 1568 1320
Size: 248 3642
KpnI G_GTAC'C
Cuts at: 910 910
Size: 3890
MluI A'CGCG_T
Cuts at: 3323 3323
Size: 3890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 2561 2745 479
Size: 2082 184 1624
Fragments arranged by size:
2082 1624 184
MscI TGG'CCA
Cuts at: 623 803 1651 623
Size: 180 848 2862
Fragments arranged by size:
2862 848 180
MslI CAynn'nnrTG
Cuts at: 1879 2006 1879
Size: 127 3763
MspA1I CmG'CkG
Cuts at: 160 575 1001 1315 1675 2688 2933 160
Size: 415 426 314 360 1013 245 1117
Fragments arranged by size:
1117 1013 426 415 360 314 245
NaeI GCC'GGC
Cuts at: 425 2071 425
Size: 1646 2244
NarI GG'CG_CC
Cuts at: 1321 1569 1321
Size: 248 3642
NgoMIV G'CCGG_C
Cuts at: 423 2069 423
Size: 1646 2244
NruI TCG'CGA
Cuts at: 281 281
Size: 3890
NspI r_CATG'y
Cuts at: 480 943 1443 2350 480
Size: 463 500 907 2020
Fragments arranged by size:
2020 907 500 463
PasI CC'CwG_GG
Cuts at: 196 846 196
Size: 650 3240
PciI A'CATG_T
Cuts at: 2346 2346
Size: 3890
PflMI CCAn_nnn'nTGG
Cuts at: 531 531
Size: 3890
PfoI T'CCnGG_A
Cuts at: 553 553
Size: 3890
PmeI GTTT'AAAC
Cuts at: 889 889
Size: 3890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3858
PpuMI rG'GwC_Cy
Cuts at: 200 200
Size: 3890
PspOMI G'GGCC_C
Cuts at: 192 290 717 842 192
Size: 98 427 125 3240
Fragments arranged by size:
3240 427 125 98
PstI C_TGCA'G
Cuts at: 417 687 716 937 1622 417
Size: 270 29 221 685 2685
Fragments arranged by size:
2685 685 270 221 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3890
PvuII CAG'CTG
Cuts at: 1315 1675 1315
Size: 360 3530
RsrII CG'GwC_CG
Cuts at: 2085 2085
Size: 3890
SacI G_AGCT'C
Cuts at: 904 904
Size: 3890
SalI G'TCGA_C
Cuts at: 927 927
Size: 3890
SanDI GG'GwC_CC
Cuts at: 200 200
Size: 3890
SapI GCTCTTCn'nnn_
Cuts at: 1913 2123 1913
Size: 210 3680
SbfI CC_TGCA'GG
Cuts at: 937 937
Size: 3890
ScaI AGT'ACT
Cuts at: 3407 3407
Size: 3890
SfcI C'TryA_G
Cuts at: 33 413 683 712 933 1618 2611 2802 33
Size: 380 270 29 221 685 993 191 1121
Fragments arranged by size:
1121 993 685 380 270 221 191 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 436 436
Size: 3890
SfoI GGC'GCC
Cuts at: 1322 1570 1322
Size: 248 3642
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3890
SmaI CCC'GGG
Cuts at: 460 912 460
Size: 452 3438
SmlI C'TyrA_G
Cuts at: 247 1045 1960 2452 2714 247
Size: 798 915 492 262 1423
Fragments arranged by size:
1423 915 798 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3890
SphI G_CATG'C
Cuts at: 943 1443 943
Size: 500 3390
StuI AGG'CCT
Cuts at: 439 439
Size: 3890
StyI C'CwwG_G
Cuts at: 635 735 1024 1378 635
Size: 100 289 354 3147
Fragments arranged by size:
3147 354 289 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 432 1473 1606 2065 2274 432
Size: 1041 133 459 209 2048
Fragments arranged by size:
2048 1041 459 209 133
TatI w'GTAC_w
Cuts at: 3405 3405
Size: 3890
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 3407 3439 616
Size: 32 2759 32 1067
Fragments arranged by size:
2759 1067 32 32
Tth111I GACn'n_nGTC
Cuts at: 1687 1687
Size: 3890
XbaI T'CTAG_A
Cuts at: 921 921
Size: 3890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1385 1385
Size: 3890
XmaI C'CCGG_G
Cuts at: 458 910 458
Size: 452 3438
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI BbsI
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI