Restriction map for
pF1KE0513
(Circular) (Six-base) MAPSORT of: pF1KE0513.seq Check: 7729 from: 1 to: 4106
pF1KE0513 4106 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 698 698
Size: 4106
AccI GT'mk_AC
Cuts at: 520 1144 520
Size: 624 3482
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 584 3946 584
Size: 3362 744
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 973 1010 1931 2363 3110 3378 649
Size: 324 37 921 432 747 268 1377
Fragments arranged by size:
1377 921 747 432 324 268 37
AfeI AGC'GCT
Cuts at: 553 553
Size: 4106
AflIII A'CryG_T
Cuts at: 320 1458 2562 3539 320
Size: 1138 1104 977 887
Fragments arranged by size:
1138 1104 977 887
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AhdI GACnn_n'nnGTC
Cuts at: 516 516
Size: 4106
AleI CACnn'nnGTG
Cuts at: 430 430
Size: 4106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 307 339 103
Size: 32 172 32 3870
Fragments arranged by size:
3870 172 32 32
AlwNI CAG_nnn'CTG
Cuts at: 360 777 807 1411 2978 360
Size: 417 30 604 1567 1488
Fragments arranged by size:
1567 1488 604 417 30
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 232 886 1110 3459 3715 232
Size: 654 224 2349 256 623
Fragments arranged by size:
2349 654 623 256 224
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 286 1126 286
Size: 840 3266
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 161 179 335 1095 1120 2150 161
Size: 18 156 760 25 1030 2117
Fragments arranged by size:
2117 1030 760 156 25 18
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 970 1218 970
Size: 248 3858
Bme1580I G_kGCm'C
Cuts at: 1731 1824 2880 1731
Size: 93 1056 2957
Fragments arranged by size:
2957 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 810 1318 1399 1734 3878 810
Size: 508 81 335 2144 1038
Fragments arranged by size:
2144 1038 508 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 2473 195
Size: 2278 1828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 457 484 964 331
Size: 126 27 480 3473
Fragments arranged by size:
3473 480 126 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 1282 2161 2653 2951 355
Size: 927 879 492 298 1510
Fragments arranged by size:
1510 927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 695 1081 1537 1785 2487 3983 695
Size: 386 456 248 702 1496 818
Fragments arranged by size:
1496 818 702 456 386 248
BsaWI w'CCGG_w
Cuts at: 449 723 1295 1816 2494 2768 2915 3326 449
Size: 274 572 521 678 274 147 411 1229
Fragments arranged by size:
1229 678 572 521 411 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 109 133 139 307 337 103
Size: 6 24 6 168 30 3872
Fragments arranged by size:
3872 168 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 187 328 481 184
Size: 3 141 153 3809
Fragments arranged by size:
3809 153 141 3
BseYI C'CCAG_C
Cuts at: 162 1531 2289 2866 3362 162
Size: 1369 758 577 496 906
Fragments arranged by size:
1369 906 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 820 859 907 511
Size: 309 39 48 3710
Fragments arranged by size:
3710 309 48 39
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 293 616 1021 1034 1120 1898 2088 2880
Size: 114 323 405 13 86 778 190 792
Cuts at: 2880 179
Size: 1405
Fragments arranged by size:
1405 792 778 405 323 190 114 86
13
Bsp1286I G_dGCh'C
Cuts at: 161 179 293 335 616 1021 1034 1095 1120
Size: 18 114 42 281 405 13 61 25
Cuts at: 1120 1731 1824 1898 2088 2150 2880 161
Size: 611 93 74 190 62 730 1387
Fragments arranged by size:
1387 730 611 405 281 190 114 93
74 62 61 42 25 18 13
BspEI T'CCGG_A
Cuts at: 723 3326 723
Size: 2603 1503
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 212 1156 1370 1672 2053 140
Size: 72 944 214 302 381 2193
Fragments arranged by size:
2193 944 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 153 153
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 3214 3300 3312
Size: 1130 467 23 335 246 1012 86 12
Cuts at: 3312 3658 1
Size: 346 449
Fragments arranged by size:
1130 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1086 107
Size: 979 3127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 375 2056 2237 2400 375
Size: 1681 181 163 2081
Fragments arranged by size:
2081 1681 181 163
BtsI GCAGTG_nn'
Cuts at: 746 1349 746
Size: 603 3503
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DraIII CAC_nnn'GTG
Cuts at: 297 297
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 545 1691 1865 2256 2283 3628 545
Size: 1146 174 391 27 1345 1023
Fragments arranged by size:
1345 1146 1023 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 284 2129 2339 284
Size: 1845 210 2051
Fragments arranged by size:
2051 1845 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 177 1118 177
Size: 941 3165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 457 484 649 964 973 1010 1931 2363
Size: 126 27 165 315 9 37 921 432
Cuts at: 2363 3110 3378 331
Size: 747 268 1059
Fragments arranged by size:
1059 921 747 432 315 268 165 126
37 27 9
EcoNI CCTnn'n_nnAGG
Cuts at: 313 313
Size: 4106
EcoO109I rG'GnC_Cy
Cuts at: 225 510 963 1245 225
Size: 285 453 282 3086
Fragments arranged by size:
3086 453 285 282
EcoRI G'AATT_C
Cuts at: 886 1110 886
Size: 224 3882
EcoRV GAT'ATC
Cuts at: 996 996
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 714 2125 714
Size: 1411 2695
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 370 555 943 1540 1788 2810 3789 370
Size: 185 388 597 248 1022 979 687
Fragments arranged by size:
1022 979 687 597 388 248 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1948 1978 1980 2010 502
Size: 32 1414 30 2 30 2598
Fragments arranged by size:
2598 1414 32 30 30 2
HincII GTy'rAC
Cuts at: 768 1145 768
Size: 377 3729
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 547 1867 547
Size: 1320 2786
MslI CAynn'nnrTG
Cuts at: 430 2095 2222 430
Size: 1665 127 2314
Fragments arranged by size:
2314 1665 127
MspA1I CmG'CkG
Cuts at: 166 462 974 1217 1531 1891 2904 3149 166
Size: 296 512 243 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 512 360 314 296 245 243
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 1086 107
Size: 979 3127
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 324 1159 1659 2566 324
Size: 835 500 907 1864
Fragments arranged by size:
1864 907 835 500
PciI A'CATG_T
Cuts at: 320 2562 320
Size: 2242 1864
PflMI CCAn_nnn'nTGG
Cuts at: 360 777 360
Size: 417 3689
PfoI T'CCnGG_A
Cuts at: 217 217
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 103 135 307 339 3245 3277 103
Size: 32 172 32 2906 32 932
Fragments arranged by size:
2906 932 172 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 510 225
Size: 285 3821
PstI C_TGCA'G
Cuts at: 140 248 461 915 1153 1838 140
Size: 108 213 454 238 685 2408
Fragments arranged by size:
2408 685 454 238 213 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 462 974 1531 1891 462
Size: 512 557 360 2677
Fragments arranged by size:
2677 557 512 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 179 1120 179
Size: 941 3165
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 136 244 457 911 1149 1834 2827 3018
Size: 103 108 213 454 238 685 993 191
Cuts at: 3018 33
Size: 1121
Fragments arranged by size:
1121 993 685 454 238 213 191 108
103
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 370 1261 2176 2668 2930 370
Size: 891 915 492 262 1546
Fragments arranged by size:
1546 915 891 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 107 146 1086 1240 1594 107
Size: 39 940 154 354 2619
Fragments arranged by size:
2619 940 354 154 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 225 829 1689 1822 2281 2490 225
Size: 604 860 133 459 209 1841
Fragments arranged by size:
1841 860 604 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 588 823 1601 486
Size: 102 235 778 2991
Fragments arranged by size:
2991 778 235 102
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
ZraI GAC'GTC
Cuts at: 696 696
Size: 4106
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AlfI ApaI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI