Restriction map for
pF1KE0519
(Circular) (Six-base) MAPSORT of: pF1KE0519.seq Check: 8713 from: 1 to: 3689
pF1KE0519 3689 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1514 1946 2693 2961 1514
Size: 432 747 268 2242
Fragments arranged by size:
2242 747 432 268
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 2077 2077
Size: 3689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 607
Size: 32 3657
AlwNI CAG_nnn'CTG
Cuts at: 627 994 2561 627
Size: 367 1567 1755
Fragments arranged by size:
1755 1567 367
ApaI G_GGCC'C
Cuts at: 125 167 125
Size: 42 3647
ApaLI G'TGCA_C
Cuts at: 2459 2459
Size: 3689
ApoI r'AATT_y
Cuts at: 693 3042 3298 693
Size: 2349 256 1084
Fragments arranged by size:
2349 1084 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 709 709
Size: 3689
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 705 1119 1367 1402 705
Size: 414 248 35 2992
Fragments arranged by size:
2992 414 248 35
BanII G_rGCy'C
Cuts at: 125 167 703 1733 125
Size: 42 536 1030 2081
Fragments arranged by size:
2081 1030 536 42
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BbsI GAAGACnn'nnnn_
Cuts at: 427 427
Size: 3689
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2348 3689 1587
Size: 761 1341 1587
Fragments arranged by size:
1587 1341 761
BclI T'GATC_A
Cuts at: 659 1209 659
Size: 550 3139
BglI GCCn_nnn'nGGC
Cuts at: 269 269
Size: 3689
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 801 801
Size: 3689
Bme1580I G_kGCm'C
Cuts at: 125 167 1314 1407 2463 125
Size: 42 1147 93 1056 1351
Fragments arranged by size:
1351 1147 1056 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 161 407 600 901 982 1317 3461 161
Size: 246 193 301 81 335 2144 389
Fragments arranged by size:
2144 389 335 301 246 193 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 3689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 569 135
Size: 434 3255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 865 1744 2236 2534 422
Size: 443 879 492 298 1577
Fragments arranged by size:
1577 879 492 443 298
BsaI GGTCTCn'nnnn_
Cuts at: 304 3393 304
Size: 3089 600
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 1120 1368 2070 3566 1120
Size: 248 702 1496 1243
Fragments arranged by size:
1496 1243 702 248
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2351 2498 2909 878
Size: 521 678 274 147 411 1658
Fragments arranged by size:
1658 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 419 572 419
Size: 153 3536
BseYI C'CCAG_C
Cuts at: 1114 1872 2449 2945 1114
Size: 758 577 496 1858
Fragments arranged by size:
1858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 176
Size: 3689
BsiEI CG_ry'CG
Cuts at: 104 1231 1277 2485 104
Size: 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 46
BsiHKAI G_wGCw'C
Cuts at: 703 1481 1671 2463 703
Size: 778 190 792 1929
Fragments arranged by size:
1929 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 125 167 703 1314 1407 1481 1671 1733 2463
Size: 42 536 611 93 74 190 62 730
Cuts at: 2463 125
Size: 1351
Fragments arranged by size:
1351 730 611 536 190 93 74 62
42
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 197 1981 2035 3519 3684 50
Size: 147 1784 54 1484 165 55
Fragments arranged by size:
1784 1484 165 147 55 54
BsrDI GCAATG_nn'
Cuts at: 186 1601 186
Size: 1415 2274
BsrFI r'CCGG_y
Cuts at: 1687 1868 2077 1687
Size: 181 209 3299
Fragments arranged by size:
3299 209 181
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 230 1066 230
Size: 836 2853
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstYI r'GATC_y
Cuts at: 1 714 1181 1204 1539 1785 2797 2883 2895
Size: 713 467 23 335 246 1012 86 12
Cuts at: 2895 3241 1
Size: 346 449
Fragments arranged by size:
1012 713 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 932 932
Size: 3689
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 332 1395 2253 332
Size: 1063 858 1768
Fragments arranged by size:
1768 1063 858
EaeI y'GGCC_r
Cuts at: 270 286 1274 1448 1839 1866 3211 270
Size: 16 988 174 391 27 1345 748
Fragments arranged by size:
1345 988 748 391 174 27 16
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 283 643 1712 1922 283
Size: 360 1069 210 2050
Fragments arranged by size:
2050 1069 360 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2207 2353 3499 1897
Size: 310 146 1146 2087
Fragments arranged by size:
2087 1146 310 146
EcoICRI GAG'CTC
Cuts at: 701 701
Size: 3689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 569 1514 1946 2693 2961 135
Size: 434 945 432 747 268 863
Fragments arranged by size:
945 863 747 434 432 268
EcoO109I rG'GnC_Cy
Cuts at: 121 122 163 408 828 121
Size: 1 41 245 420 2982
Fragments arranged by size:
2982 420 245 41 1
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3689
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 188 1123 1371 2393 3372 188
Size: 935 248 1022 979 505
Fragments arranged by size:
1022 979 935 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 353 385 414 446 1531 1561 1563 1593 353
Size: 32 29 32 1085 30 2 30 2449
Fragments arranged by size:
2449 1085 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 287 2360 2544 287
Size: 2073 184 1432
Fragments arranged by size:
2073 1432 184
MscI TGG'CCA
Cuts at: 272 1450 272
Size: 1178 2511
MslI CAynn'nnrTG
Cuts at: 1678 1805 1678
Size: 127 3562
MspA1I CmG'CkG
Cuts at: 589 800 1114 1474 2487 2732 589
Size: 211 314 360 1013 245 1546
Fragments arranged by size:
1546 1013 360 314 245 211
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NcoI C'CATG_G
Cuts at: 107 107
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NspI r_CATG'y
Cuts at: 742 1242 2149 742
Size: 500 907 2282
Fragments arranged by size:
2282 907 500
PasI CC'CwG_GG
Cuts at: 595 595
Size: 3689
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PflMI CCAn_nnn'nTGG
Cuts at: 612 612
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 3689
PspOMI G'GGCC_C
Cuts at: 121 163 121
Size: 42 3647
PstI C_TGCA'G
Cuts at: 736 1421 736
Size: 685 3004
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3689
PvuII CAG'CTG
Cuts at: 589 1114 1474 589
Size: 525 360 2804
Fragments arranged by size:
2804 525 360
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 703 703
Size: 3689
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 732 1417 2410 2601 33
Size: 699 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 191
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 437 844 1759 2251 2513 437
Size: 407 915 492 262 1613
Fragments arranged by size:
1613 915 492 407 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StyI C'CwwG_G
Cuts at: 107 245 823 1177 107
Size: 138 578 354 2619
Fragments arranged by size:
2619 578 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1272 1405 1864 2073 1272
Size: 133 459 209 2888
Fragments arranged by size:
2888 459 209 133
TatI w'GTAC_w
Cuts at: 3204 3204
Size: 3689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 623 655 3206 3238 623
Size: 32 2551 32 1074
Fragments arranged by size:
2551 1074 32 32
Tth111I GACn'n_nGTC
Cuts at: 367 1486 367
Size: 1119 2570
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 514 1184 514
Size: 670 3019
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI