Restriction map for
pF1KE0520
(Circular) (Six-base) MAPSORT of: pF1KE0520.seq Check: 2147 from: 1 to: 4163
pF1KE0520 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 703 703
Size: 4163
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1988 2420 3167 3435 577
Size: 1411 432 747 268 1305
Fragments arranged by size:
1411 1305 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AleI CACnn'nnGTG
Cuts at: 270 982 270
Size: 712 3451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 711 711
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 172 794 1468 3035 172
Size: 622 674 1567 1300
Fragments arranged by size:
1567 1300 674 622
ApaI G_GGCC'C
Cuts at: 197 249 197
Size: 52 4111
ApaLI G'TGCA_C
Cuts at: 325 2933 325
Size: 2608 1555
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 199 248 1183 199
Size: 49 935 3179
Fragments arranged by size:
3179 935 49
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 404 1179 1593 1841 1876 404
Size: 775 414 248 35 2691
Fragments arranged by size:
2691 775 414 248 35
BanII G_rGCy'C
Cuts at: 197 249 1177 2207 197
Size: 52 928 1030 2153
Fragments arranged by size:
2153 1030 928 52
BbeI G_GCGC'C
Cuts at: 408 1597 1845 408
Size: 1189 248 2726
Fragments arranged by size:
2726 1189 248
BbvCI CC'TCA_GC
Cuts at: 150 1036 150
Size: 886 3277
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 253 559 1108 253
Size: 306 549 3308
Fragments arranged by size:
3308 549 306
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 886 1275 886
Size: 389 3774
Bme1580I G_kGCm'C
Cuts at: 197 249 329 402 503 732 1035 1788 1881
Size: 52 80 73 101 229 303 753 93
Cuts at: 1881 2937 197
Size: 1056 1423
Fragments arranged by size:
1423 1056 753 303 229 101 93 80
73 52
BmgBI CAC'GTC
Cuts at: 464 963 464
Size: 499 3664
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 844 665
Size: 179 3984
Bpu10I CC'TnA_GC
Cuts at: 150 182 1036 150
Size: 32 854 3277
Fragments arranged by size:
3277 854 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1339 2218 2710 3008 694
Size: 645 879 492 298 1849
Fragments arranged by size:
1849 879 645 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1044 1516 1044
Size: 472 3691
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 405 1594 1842 2544 4040 405
Size: 1189 248 702 1496 528
Fragments arranged by size:
1496 1189 702 528 248
BsaWI w'CCGG_w
Cuts at: 653 1352 1873 2551 2825 2972 3383 653
Size: 699 521 678 274 147 411 1433
Fragments arranged by size:
1433 699 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 757
Size: 4163
BseYI C'CCAG_C
Cuts at: 190 570 787 1144 1588 2346 2923 3419 190
Size: 380 217 357 444 758 577 496 934
Fragments arranged by size:
934 758 577 496 444 380 357 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 830 346
Size: 484 3679
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 183 329 1177 1955 2145 2937 131
Size: 52 146 848 778 190 792 1357
Fragments arranged by size:
1357 848 792 778 190 146 52
Bsp1286I G_dGCh'C
Cuts at: 131 183 197 249 329 402 503 732 1035
Size: 52 14 52 80 73 101 229 303
Cuts at: 1035 1177 1788 1881 1955 2145 2207 2937 131
Size: 142 611 93 74 190 62 730 1357
Fragments arranged by size:
1357 730 611 303 229 190 142 101
93 80 74 73 62 52 52 14
BspEI T'CCGG_A
Cuts at: 653 3383 653
Size: 2730 1433
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 1213 1427 1729 2110 703
Size: 510 214 302 381 2756
Fragments arranged by size:
2756 510 381 302 214
BsrBI CCG'CTC
Cuts at: 50 615 2455 2509 3993 4158 50
Size: 565 1840 54 1484 165 55
Fragments arranged by size:
1840 1484 565 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 667 2161 2342 2551 667
Size: 1494 181 209 2279
Fragments arranged by size:
2279 1494 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 428 1540 428
Size: 1112 3051
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstEII G'GTnAC_C
Cuts at: 759 759
Size: 4163
BstYI r'GATC_y
Cuts at: 1 314 1188 1655 1678 2013 2259 3271 3357
Size: 313 874 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 874 467 449 346 335 313 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 221 490 221
Size: 269 3894
BtgI C'CryG_G
Cuts at: 257 467 605 702 980 257
Size: 210 138 97 278 3440
Fragments arranged by size:
3440 278 210 138 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1035 2113 2294 2457 1035
Size: 1078 181 163 2741
Fragments arranged by size:
2741 1078 181 163
BtsI GCAGTG_nn'
Cuts at: 848 1406 848
Size: 558 3605
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 240 254 599 917 1748 1922 2313 2340 3685
Size: 14 345 318 831 174 391 27 1345
Cuts at: 3685 240
Size: 718
Fragments arranged by size:
1345 831 718 391 345 318 174 27
14
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1146 2371 2681 2827 3973 1146
Size: 1225 310 146 1146 1336
Fragments arranged by size:
1336 1225 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 665 844 1988 2420 3167 3435 577
Size: 88 179 1144 432 747 268 1305
Fragments arranged by size:
1305 1144 747 432 268 179 88
EcoNI CCTnn'n_nnAGG
Cuts at: 736 736
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 194 225 1302 194
Size: 31 1077 3055
Fragments arranged by size:
3055 1077 31
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 333 2182 333
Size: 1849 2314
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 408 565 1597 1845 2867 3846 408
Size: 157 1032 248 1022 979 725
Fragments arranged by size:
1032 1022 979 725 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2005 2035 2037 2067 2005
Size: 30 2 30 4101
Fragments arranged by size:
4101 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 404 1593 1841 404
Size: 1189 248 2726
Fragments arranged by size:
2726 1189 248
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 256 919 1924 256
Size: 663 1005 2495
Fragments arranged by size:
2495 1005 663
MslI CAynn'nnrTG
Cuts at: 134 270 370 466 982 2152 2279 134
Size: 136 100 96 516 1170 127 2018
Fragments arranged by size:
2018 1170 516 136 127 100 96
MspA1I CmG'CkG
Cuts at: 116 172 704 890 1274 1588 1948 2961 3206
Size: 56 532 186 384 314 360 1013 245
Cuts at: 3206 116
Size: 1073
Fragments arranged by size:
1073 1013 532 384 360 314 245 186
56
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 405 1594 1842 405
Size: 1189 248 2726
Fragments arranged by size:
2726 1189 248
NcoI C'CATG_G
Cuts at: 257 467 605 257
Size: 210 138 3815
Fragments arranged by size:
3815 210 138
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 297 369 389 1216 1716 2623 297
Size: 72 20 827 500 907 1837
Fragments arranged by size:
1837 907 827 500 72 20
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 966 966
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PshAI GACnn'nnGTC
Cuts at: 711 711
Size: 4163
PspOMI G'GGCC_C
Cuts at: 193 245 193
Size: 52 4111
PstI C_TGCA'G
Cuts at: 637 740 1210 1895 637
Size: 103 470 685 2905
Fragments arranged by size:
2905 685 470 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 172 890 1588 1948 172
Size: 718 698 360 2387
Fragments arranged by size:
2387 718 698 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SacII CC_GC'GG
Cuts at: 705 705
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 633 736 1206 1891 2884 3075 33
Size: 600 103 470 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 470 191 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 253 253
Size: 4163
SfoI GGC'GCC
Cuts at: 406 1595 1843 406
Size: 1189 248 2726
Fragments arranged by size:
2726 1189 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 709 1318 2233 2725 2987 709
Size: 609 915 492 262 1885
Fragments arranged by size:
1885 915 609 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 389 1216 1716 389
Size: 827 500 2836
Fragments arranged by size:
2836 827 500
StyI C'CwwG_G
Cuts at: 257 467 605 1297 1651 257
Size: 210 138 692 354 2769
Fragments arranged by size:
2769 692 354 210 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 1658 264
Size: 1394 2769
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI