Restriction map for
pF1KE0524
(Circular) (Six-base) MAPSORT of: pF1KE0524.seq Check: 773 from: 1 to: 3830
pF1KE0524 3830 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 132
Size: 3830
AatII G_ACGT'C
Cuts at: 671 671
Size: 3830
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1655 2087 2834 3102 712
Size: 943 432 747 268 1440
Fragments arranged by size:
1440 943 747 432 268
AflIII A'CryG_T
Cuts at: 497 1182 2286 3263 497
Size: 685 1104 977 1064
Fragments arranged by size:
1104 1064 977 685
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2702 1135
Size: 1567 2263
ApaI G_GGCC'C
Cuts at: 305 305
Size: 3830
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 834 3183 3439 834
Size: 2349 256 1225
Fragments arranged by size:
2349 1225 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 518 850 518
Size: 332 3498
BamHI G'GATC_C
Cuts at: 855 855
Size: 3830
BanI G'GyrC_C
Cuts at: 144 558 816 846 1260 1508 1543 144
Size: 414 258 30 414 248 35 2431
Fragments arranged by size:
2431 414 414 258 248 35 30
BanII G_rGCy'C
Cuts at: 212 305 373 812 844 1874 212
Size: 93 68 439 32 1030 2168
Fragments arranged by size:
2168 1030 439 93 68 32
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BbsI GAAGACnn'nnnn_
Cuts at: 393 663 393
Size: 270 3560
BbvCI CC'TCA_GC
Cuts at: 214 214
Size: 3830
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 481 788 481
Size: 307 3523
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 305 1455 1548 2604 305
Size: 1150 93 1056 1531
Fragments arranged by size:
1531 1150 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 291 682 707 226
Size: 65 391 25 3349
Fragments arranged by size:
3349 391 65 25
Bpu10I CC'TnA_GC
Cuts at: 214 214
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 498 1183 498
Size: 685 3145
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 422 668 1261 1509 2211 3707 422
Size: 246 593 248 702 1496 545
Fragments arranged by size:
1496 702 593 545 248 246
BsaWI w'CCGG_w
Cuts at: 254 1019 1540 2218 2492 2639 3050 254
Size: 765 521 678 274 147 411 1034
Fragments arranged by size:
1034 765 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 3830
BseYI C'CCAG_C
Cuts at: 438 600 627 744 1255 2013 2590 3086 438
Size: 162 27 117 511 758 577 496 1182
Fragments arranged by size:
1182 758 577 511 496 162 117 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3830
BsiEI CG_ry'CG
Cuts at: 104 131 384 392 1372 1418 2626 104
Size: 27 253 8 980 46 1208 1308
Fragments arranged by size:
1308 1208 980 253 46 27 8
BsiHKAI G_wGCw'C
Cuts at: 212 812 844 1622 1812 2604 212
Size: 600 32 778 190 792 1438
Fragments arranged by size:
1438 792 778 600 190 32
Bsp1286I G_dGCh'C
Cuts at: 212 305 373 812 844 1455 1548 1622 1812
Size: 93 68 439 32 611 93 74 190
Cuts at: 1812 1874 2604 212
Size: 62 730 1438
Fragments arranged by size:
1438 730 611 439 190 93 93 74
68 62 32
BspEI T'CCGG_A
Cuts at: 254 3050 254
Size: 2796 1034
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 632 880 1094 1396 1777 132
Size: 500 248 214 302 381 2185
Fragments arranged by size:
2185 500 381 302 248 214
BsrBI CCG'CTC
Cuts at: 50 2122 2176 3660 3825 50
Size: 2072 54 1484 165 55
Fragments arranged by size:
2072 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 147 1828 2009 2218 147
Size: 1681 181 209 1759
Fragments arranged by size:
1759 1681 209 181
BssSI C'ACGA_G
Cuts at: 616 2101 2459 616
Size: 1485 358 1987
Fragments arranged by size:
1987 1485 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 3830
BstYI r'GATC_y
Cuts at: 1 855 1322 1345 1680 1926 2938 3024 3036
Size: 854 467 23 335 246 1012 86 12
Cuts at: 3036 3382 1
Size: 346 449
Fragments arranged by size:
1012 854 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 194 378 107
Size: 87 184 3559
Fragments arranged by size:
3559 184 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1073
Size: 3830
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 128 381 389 482 1415 1589 1980 2007 3352
Size: 253 8 93 933 174 391 27 1345
Cuts at: 3352 128
Size: 606
Fragments arranged by size:
1345 933 606 391 253 174 93 27
8
EagI C'GGCC_G
Cuts at: 128 381 389 1415 128
Size: 253 8 1026 2543
Fragments arranged by size:
2543 1026 253 8
EarI CTCTTCn'nnn_
Cuts at: 298 340 359 418 1853 2063 298
Size: 42 19 59 1435 210 2065
Fragments arranged by size:
2065 1435 210 59 42 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 210 810 842 210
Size: 600 32 3198
Fragments arranged by size:
3198 600 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 291 682 707 712 1655 2087 2834 3102
Size: 65 391 25 5 943 432 747 268
Cuts at: 3102 226
Size: 954
Fragments arranged by size:
954 943 747 432 391 268 65 25
5
EcoNI CCTnn'n_nnAGG
Cuts at: 469 469
Size: 3830
EcoO109I rG'GnC_Cy
Cuts at: 302 474 741 969 302
Size: 172 267 228 3163
Fragments arranged by size:
3163 267 228 172
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 580 1264 1512 2534 3513 580
Size: 684 248 1022 979 897
Fragments arranged by size:
1022 979 897 684 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1702 1704 1734 1672
Size: 30 2 30 3768
Fragments arranged by size:
3768 30 30 2
HincII GTy'rAC
Cuts at: 531 869 531
Size: 338 3492
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2685 2501
Size: 184 3646
MscI TGG'CCA
Cuts at: 484 1591 484
Size: 1107 2723
MslI CAynn'nnrTG
Cuts at: 484 490 616 1819 1946 484
Size: 6 126 1203 127 2368
Fragments arranged by size:
2368 1203 127 126 6
MspA1I CmG'CkG
Cuts at: 196 204 380 748 941 1255 1615 2628 2873
Size: 8 176 368 193 314 360 1013 245
Cuts at: 2873 196
Size: 1153
Fragments arranged by size:
1153 1013 368 360 314 245 193 176
8
NaeI GCC'GGC
Cuts at: 149 2011 149
Size: 1862 1968
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 147 2009 147
Size: 1862 1968
NotI GC'GGCC_GC
Cuts at: 381 381
Size: 3830
NspI r_CATG'y
Cuts at: 333 360 883 1383 2290 333
Size: 27 523 500 907 1873
Fragments arranged by size:
1873 907 523 500 27
PasI CC'CwG_GG
Cuts at: 306 793 306
Size: 487 3343
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3830
PfoI T'CCnGG_A
Cuts at: 659 659
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PspOMI G'GGCC_C
Cuts at: 301 301
Size: 3830
PstI C_TGCA'G
Cuts at: 143 707 877 1562 143
Size: 564 170 685 2411
Fragments arranged by size:
2411 685 564 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 204 748 1255 1615 204
Size: 544 507 360 2419
Fragments arranged by size:
2419 544 507 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 212 812 844 212
Size: 600 32 3198
Fragments arranged by size:
3198 600 32
SacII CC_GC'GG
Cuts at: 197 381 197
Size: 184 3646
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 340 1853 2063 340
Size: 1513 210 2107
Fragments arranged by size:
2107 1513 210
SbfI CC_TGCA'GG
Cuts at: 143 877 143
Size: 734 3096
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SfcI C'TryA_G
Cuts at: 33 139 703 873 1558 2551 2742 33
Size: 106 564 170 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 191 170 106
SfiI GGCCn_nnn'nGGCC
Cuts at: 481 481
Size: 3830
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 360 883 1383 360
Size: 523 500 2807
Fragments arranged by size:
2807 523 500
StyI C'CwwG_G
Cuts at: 107 571 964 1318 107
Size: 464 393 354 2619
Fragments arranged by size:
2619 464 393 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 3345 3345
Size: 3830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 397 1627 397
Size: 1230 2600
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
ZraI GAC'GTC
Cuts at: 669 669
Size: 3830
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI