Restriction map for
pF1KE0525
(Circular) (Six-base) MAPSORT of: pF1KE0525.seq Check: 8621 from: 1 to: 4265
pF1KE0525 4265 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 164 964 1143 164
Size: 800 179 3286
Fragments arranged by size:
3286 800 179
AccI GT'mk_AC
Cuts at: 1303 1303
Size: 4265
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2522 3269 3537 2090
Size: 432 747 268 2818
Fragments arranged by size:
2818 747 432 268
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AleI CACnn'nnGTG
Cuts at: 1249 1249
Size: 4265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 4265
AlwNI CAG_nnn'CTG
Cuts at: 557 567 1072 1570 3137 557
Size: 10 505 498 1567 1685
Fragments arranged by size:
1685 1567 505 498 10
ApaI G_GGCC'C
Cuts at: 545 545
Size: 4265
ApaLI G'TGCA_C
Cuts at: 322 3035 322
Size: 2713 1552
ApoI r'AATT_y
Cuts at: 517 1269 3618 3874 517
Size: 752 2349 256 908
Fragments arranged by size:
2349 908 752 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 1005 1053 1285 1005
Size: 48 232 3985
Fragments arranged by size:
3985 232 48
BamHI G'GATC_C
Cuts at: 1290 1290
Size: 4265
BanI G'GyrC_C
Cuts at: 815 976 1281 1695 1943 1978 815
Size: 161 305 414 248 35 3102
Fragments arranged by size:
3102 414 305 248 161 35
BanII G_rGCy'C
Cuts at: 545 1214 1279 2309 545
Size: 669 65 1030 2501
Fragments arranged by size:
2501 1030 669 65
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 4265
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglI GCCn_nnn'nGGC
Cuts at: 373 373
Size: 4265
BglII A'GATC_T
Cuts at: 1780 3817 1780
Size: 2037 2228
BlpI GC'TnA_GC
Cuts at: 1127 1377 1127
Size: 250 4015
Bme1580I G_kGCm'C
Cuts at: 326 545 979 1890 1983 3039 326
Size: 219 434 911 93 1056 1552
Fragments arranged by size:
1552 1056 911 434 219 93
BmrI ACTGGGnnnn_n'
Cuts at: 1477 1558 1893 4037 1477
Size: 81 335 2144 1705
Fragments arranged by size:
2144 1705 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1020 2632 1020
Size: 1612 2653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 234 154
Size: 80 4185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 390 3969 390
Size: 3579 686
BsaAI yAC'GTr
Cuts at: 327 1618 327
Size: 1291 2974
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 667 1454 1975 2653 2927 3074 3485 667
Size: 787 521 678 274 147 411 1447
Fragments arranged by size:
1447 787 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 310
Size: 4265
BseYI C'CCAG_C
Cuts at: 1690 2448 3025 3521 1690
Size: 758 577 496 2434
Fragments arranged by size:
2434 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 326 894 986 1214 1279 2057 2247 3039 326
Size: 568 92 228 65 778 190 792 1552
Fragments arranged by size:
1552 792 778 568 228 190 92 65
BsmI GAATG_Cn'
Cuts at: 145 145
Size: 4265
BsmBI CGTCTCn'nnnn_
Cuts at: 300 300
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 326 545 894 979 986 1214 1279 1890 1983
Size: 219 349 85 7 228 65 611 93
Cuts at: 1983 2057 2247 2309 3039 326
Size: 74 190 62 730 1552
Fragments arranged by size:
1552 730 611 349 228 219 190 93
85 74 65 62 7
BspEI T'CCGG_A
Cuts at: 667 3485 667
Size: 2818 1447
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 964 1143 1178 1315 1529 1831 2212 164
Size: 800 179 35 137 214 302 381 2217
Fragments arranged by size:
2217 800 381 302 214 179 137 35
BsrBI CCG'CTC
Cuts at: 50 162 2557 2611 4095 4260 50
Size: 112 2395 54 1484 165 55
Fragments arranged by size:
2395 1484 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 437 935 2177 437
Size: 498 1242 2525
Fragments arranged by size:
2525 1242 498
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BsrGI T'GTAC_A
Cuts at: 839 839
Size: 4265
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 937 1642 937
Size: 705 3560
BstBI TT'CG_AA
Cuts at: 239 2626 239
Size: 2387 1878
BstXI CCAn_nnnn'nTGG
Cuts at: 1036 1036
Size: 4265
BstYI r'GATC_y
Cuts at: 1 1290 1757 1780 2115 2361 3373 3459 3471
Size: 1289 467 23 335 246 1012 86 12
Cuts at: 3471 3817 1
Size: 346 449
Fragments arranged by size:
1289 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 650 107
Size: 543 3722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 945 1508 945
Size: 563 3702
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 878 1850 2024 2415 2442 3787 878
Size: 972 174 391 27 1345 1356
Fragments arranged by size:
1356 1345 972 391 174 27
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 2783 2929 4075 2473
Size: 310 146 1146 2663
Fragments arranged by size:
2663 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1212 1277 1212
Size: 65 4200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 234 2090 2522 3269 3537 154
Size: 80 1856 432 747 268 882
Fragments arranged by size:
1856 882 747 432 268 80
EcoO109I rG'GnC_Cy
Cuts at: 1404 1404
Size: 4265
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2284 2284
Size: 4265
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 119 1699 1947 2969 3948 119
Size: 1580 248 1022 979 436
Fragments arranged by size:
1580 1022 979 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 431 463 2107 2137 2139 2169 112
Size: 32 287 32 1644 30 2 30 2208
Fragments arranged by size:
2208 1644 287 32 32 30 30 2
HincII GTy'rAC
Cuts at: 444 731 1304 444
Size: 287 573 3405
Fragments arranged by size:
3405 573 287
HindIII A'AGCT_T
Cuts at: 214 233 502 214
Size: 19 269 3977
Fragments arranged by size:
3977 269 19
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1256 2936 3120 1256
Size: 1680 184 2401
Fragments arranged by size:
2401 1680 184
MscI TGG'CCA
Cuts at: 2026 2026
Size: 4265
MslI CAynn'nnrTG
Cuts at: 589 629 1249 2254 2381 589
Size: 40 620 1005 127 2473
Fragments arranged by size:
2473 1005 620 127 40
MspA1I CmG'CkG
Cuts at: 292 303 1376 1690 2050 3063 3308 292
Size: 11 1073 314 360 1013 245 1249
Fragments arranged by size:
1249 1073 1013 360 314 245 11
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 107
Size: 4265
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 1318 1818 2725 1318
Size: 500 907 2858
Fragments arranged by size:
2858 907 500
PasI CC'CwG_GG
Cuts at: 227 227
Size: 4265
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PflMI CCAn_nnn'nTGG
Cuts at: 585 585
Size: 4265
PfoI T'CCnGG_A
Cuts at: 458 605 458
Size: 147 4118
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PmlI CAC'GTG
Cuts at: 327 327
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4233
PspOMI G'GGCC_C
Cuts at: 541 541
Size: 4265
PspXI vC'TCGA_Gb
Cuts at: 1053 1053
Size: 4265
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 790 822 790
Size: 32 4233
PstI C_TGCA'G
Cuts at: 272 1312 1997 272
Size: 1040 685 2540
Fragments arranged by size:
2540 1040 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 303 1690 2050 303
Size: 1387 360 2518
Fragments arranged by size:
2518 1387 360
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 1214 1279 1214
Size: 65 4200
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 3782 3782
Size: 4265
SfcI C'TryA_G
Cuts at: 33 268 1236 1308 1993 2986 3177 33
Size: 235 968 72 685 993 191 1121
Fragments arranged by size:
1121 993 968 685 235 191 72
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 1053 1420 2335 2827 3089 1053
Size: 367 915 492 262 2229
Fragments arranged by size:
2229 915 492 367 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StyI C'CwwG_G
Cuts at: 107 508 1399 1753 107
Size: 401 891 354 2619
Fragments arranged by size:
2619 891 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 1848 1981 2440 2649 703
Size: 1145 133 459 209 2319
Fragments arranged by size:
2319 1145 459 209 133
TatI w'GTAC_w
Cuts at: 839 3780 839
Size: 2941 1324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 3782 3814 622
Size: 32 3128 32 1073
Fragments arranged by size:
3128 1073 32 32
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 1760 969
Size: 791 3474
XhoI C'TCGA_G
Cuts at: 1053 1053
Size: 4265
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PpuMI PshAI PsiI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI