Restriction map for
pF1KE0528
(Circular) (Six-base) MAPSORT of: pF1KE0528.seq Check: 8893 from: 1 to: 3977
pF1KE0528 3977 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 338 1015 338
Size: 677 3300
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1802 2234 2981 3249 178
Size: 1624 432 747 268 906
Fragments arranged by size:
1624 906 747 432 268
AfeI AGC'GCT
Cuts at: 445 445
Size: 3977
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 853 863 853
Size: 10 3967
AlwNI CAG_nnn'CTG
Cuts at: 384 765 952 1282 2849 384
Size: 381 187 330 1567 1512
Fragments arranged by size:
1567 1512 381 330 187
ApaI G_GGCC'C
Cuts at: 404 404
Size: 3977
ApaLI G'TGCA_C
Cuts at: 178 2747 178
Size: 2569 1408
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 236 484 997 236
Size: 248 513 3216
Fragments arranged by size:
3216 513 248
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 804 993 1407 1655 1690 804
Size: 189 414 248 35 3091
Fragments arranged by size:
3091 414 248 189 35
BanII G_rGCy'C
Cuts at: 322 404 485 796 991 2021 322
Size: 82 81 311 195 1030 2278
Fragments arranged by size:
2278 1030 311 195 82 81
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 318 318
Size: 3977
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BglI GCCn_nnn'nGGC
Cuts at: 408 408
Size: 3977
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 182 404 807 1602 1695 2751 182
Size: 222 403 795 93 1056 1408
Fragments arranged by size:
1408 1056 795 403 222 93
BmrI ACTGGGnnnn_n'
Cuts at: 316 1189 1270 1605 3749 316
Size: 873 81 335 2144 544
Fragments arranged by size:
2144 873 544 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 394 694 310
Size: 84 300 3593
Fragments arranged by size:
3593 300 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 1408 1656 2358 3854 1408
Size: 248 702 1496 1531
Fragments arranged by size:
1531 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 877 907 877
Size: 30 3947
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 310 766 307
Size: 3 456 3518
Fragments arranged by size:
3518 456 3
BseYI C'CCAG_C
Cuts at: 580 1402 2160 2737 3233 580
Size: 822 758 577 496 1324
Fragments arranged by size:
1324 822 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 340
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 182 548 991 1769 1959 2751 182
Size: 366 443 778 190 792 1408
Fragments arranged by size:
1408 792 778 443 366 190
BsmI GAATG_Cn'
Cuts at: 201 201
Size: 3977
BsmBI CGTCTCn'nnnn_
Cuts at: 233 233
Size: 3977
Bsp1286I G_dGCh'C
Cuts at: 182 322 404 485 548 796 807 991 1602
Size: 140 82 81 63 248 11 184 611
Cuts at: 1602 1695 1769 1959 2021 2751 182
Size: 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 248 190 184 140 93
82 81 74 63 62 11
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 206 3153 206
Size: 2947 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 584 1027 1241 1543 1924 584
Size: 443 214 302 381 2637
Fragments arranged by size:
2637 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 656 893 2269 2323 3807 3972 50
Size: 606 237 1376 54 1484 165 55
Fragments arranged by size:
1484 1376 606 237 165 55 54
BsrDI GCAATG_nn'
Cuts at: 620 1889 620
Size: 1269 2708
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 463 2248 2606 463
Size: 1785 358 1834
Fragments arranged by size:
1834 1785 358
BstAPI GCAn_nnn'nTGC
Cuts at: 196 765 1354 196
Size: 569 589 2819
Fragments arranged by size:
2819 589 569
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstEII G'GTnAC_C
Cuts at: 231 523 231
Size: 292 3685
BstYI r'GATC_y
Cuts at: 1 1002 1469 1492 1827 2073 3085 3171 3183
Size: 1001 467 23 335 246 1012 86 12
Cuts at: 3183 3529 1
Size: 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 854 968 107
Size: 747 114 3116
Fragments arranged by size:
3116 747 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DraIII CAC_nnn'GTG
Cuts at: 468 468
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 1562 1736 2127 2154 3499 1562
Size: 174 391 27 1345 2040
Fragments arranged by size:
2040 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 344 704 2000 2210 344
Size: 360 1296 210 2111
Fragments arranged by size:
2111 1296 360 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 310 394 694 1802 2234 2981 3249 178
Size: 132 84 300 1108 432 747 268 906
Fragments arranged by size:
1108 906 747 432 300 268 132 84
EcoNI CCTnn'n_nnAGG
Cuts at: 160 292 376 160
Size: 132 84 3761
Fragments arranged by size:
3761 132 84
EcoO109I rG'GnC_Cy
Cuts at: 153 381 401 594 918 1116 153
Size: 228 20 193 324 198 3014
Fragments arranged by size:
3014 324 228 198 193 20
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 447 1411 1659 2681 3660 447
Size: 964 248 1022 979 764
Fragments arranged by size:
1022 979 964 764 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1819 1849 1851 1881 1819
Size: 30 2 30 3915
Fragments arranged by size:
3915 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 1738 1738
Size: 3977
MslI CAynn'nnrTG
Cuts at: 278 280 1966 2093 278
Size: 2 1686 127 2162
Fragments arranged by size:
2162 1686 127 2
MspA1I CmG'CkG
Cuts at: 407 822 1088 1402 1762 2775 3020 407
Size: 415 266 314 360 1013 245 1364
Fragments arranged by size:
1364 1013 415 360 314 266 245
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NcoI C'CATG_G
Cuts at: 107 968 107
Size: 861 3116
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 135 1030 1530 2437 135
Size: 895 500 907 1675
Fragments arranged by size:
1675 907 895 500
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PflMI CCAn_nnn'nTGG
Cuts at: 468 468
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PpuMI rG'GwC_Cy
Cuts at: 381 918 381
Size: 537 3440
PspOMI G'GGCC_C
Cuts at: 400 400
Size: 3977
PstI C_TGCA'G
Cuts at: 1024 1709 1024
Size: 685 3292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 1402 1762 1402
Size: 360 3617
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 344 2000 2210 344
Size: 1656 210 2111
Fragments arranged by size:
2111 1656 210
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 953 1020 1705 2698 2889 33
Size: 920 67 685 993 191 1121
Fragments arranged by size:
1121 993 920 685 191 67
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 135 1030 1530 135
Size: 895 500 2582
Fragments arranged by size:
2582 895 500
StyI C'CwwG_G
Cuts at: 107 170 782 968 1111 1465 107
Size: 63 612 186 143 354 2619
Fragments arranged by size:
2619 612 354 186 143 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 511 1560 1693 2152 2361 199
Size: 312 1049 133 459 209 1815
Fragments arranged by size:
1815 1049 459 312 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 3945
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1472 1472
Size: 3977
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI