Restriction map for
pF1KE0531
(Circular) (Six-base) MAPSORT of: pF1KE0531.seq Check: 4000 from: 1 to: 4346
pF1KE0531 4346 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 493 493
Size: 4346
AatII G_ACGT'C
Cuts at: 1220 1220
Size: 4346
AccI GT'mk_AC
Cuts at: 557 1220 1384 557
Size: 663 164 3519
Fragments arranged by size:
3519 663 164
Acc65I G'GTAC_C
Cuts at: 611 1301 1362 611
Size: 690 61 3595
Fragments arranged by size:
3595 690 61
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 469 1113 1268 2171 2603 3350 3618 136
Size: 333 644 155 903 432 747 268 864
Fragments arranged by size:
903 864 747 644 432 333 268 155
AfeI AGC'GCT
Cuts at: 397 397
Size: 4346
AflIII A'CryG_T
Cuts at: 138 1698 2802 3779 138
Size: 1560 1104 977 705
Fragments arranged by size:
1560 1104 977 705
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 153 1651 3218 153
Size: 1498 1567 1281
Fragments arranged by size:
1567 1498 1281
ApaI G_GGCC'C
Cuts at: 606 912 606
Size: 306 4040
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 592 905 1366 592
Size: 313 461 3572
Fragments arranged by size:
3572 461 313
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 951 984 951
Size: 33 4313
BamHI G'GATC_C
Cuts at: 387 1029 1371 387
Size: 642 342 3362
Fragments arranged by size:
3362 642 342
BanI G'GyrC_C
Cuts at: 502 611 1301 1362 1776 2024 2059 502
Size: 109 690 61 414 248 35 2789
Fragments arranged by size:
2789 690 414 248 109 61 35
BanII G_rGCy'C
Cuts at: 173 606 912 1360 2390 173
Size: 433 306 448 1030 2129
Fragments arranged by size:
2129 1030 448 433 306
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BbsI GAAGACnn'nnnn_
Cuts at: 124 1116 124
Size: 992 3354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4312
BciVI GTATCCnnnnn_n'
Cuts at: 1319 2244 3005 4346 1319
Size: 925 761 1341 1319
Fragments arranged by size:
1341 1319 925 761
BclI T'GATC_A
Cuts at: 1013 1271 1866 1013
Size: 258 595 3493
Fragments arranged by size:
3493 595 258
BglI GCCn_nnn'nGGC
Cuts at: 916 916
Size: 4346
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 606 848 912 1971 2064 3120 606
Size: 242 64 1059 93 1056 1832
Fragments arranged by size:
1832 1059 1056 242 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 1558 1639 1974 4118 1558
Size: 81 335 2144 1786
Fragments arranged by size:
2144 1786 335 81
BmtI G_CTAG'C
Cuts at: 1115 1115
Size: 4346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 1345
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1522 2401 2893 3191 550
Size: 972 879 492 298 1705
Fragments arranged by size:
1705 972 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 892 4050 892
Size: 3158 1188
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1018 1860 3897 6
Size: 1012 842 2037 455
Fragments arranged by size:
2037 1012 842 455
BsaHI Gr'CG_yC
Cuts at: 1217 1777 2025 2727 4223 1217
Size: 560 248 702 1496 1340
Fragments arranged by size:
1496 1340 702 560 248
BsaWI w'CCGG_w
Cuts at: 287 1535 2056 2734 3008 3155 3566 287
Size: 1248 521 678 274 147 411 1067
Fragments arranged by size:
1248 1067 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 1150 1180 421
Size: 30 699 30 3587
Fragments arranged by size:
3587 699 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 1155 610
Size: 545 3801
BseYI C'CCAG_C
Cuts at: 132 1334 1771 2529 3106 3602 132
Size: 1202 437 758 577 496 876
Fragments arranged by size:
1202 876 758 577 496 437
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 128 785 812 1360 2138 2328 3120 128
Size: 657 27 548 778 190 792 1354
Fragments arranged by size:
1354 792 778 657 548 190 27
BsmI GAATG_Cn'
Cuts at: 410 642 410
Size: 232 4114
Bsp1286I G_dGCh'C
Cuts at: 128 173 606 785 812 848 912 1360 1971
Size: 45 433 179 27 36 64 448 611
Cuts at: 1971 2064 2138 2328 2390 3120 128
Size: 93 74 190 62 730 1354
Fragments arranged by size:
1354 730 611 448 433 190 179 93
74 64 62 45 36 27
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 1010 3522 1010
Size: 2512 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 493 971 1329 1396 1610 1912 2293 493
Size: 478 358 67 214 302 381 2546
Fragments arranged by size:
2546 478 381 358 302 214 67
BsrBI CCG'CTC
Cuts at: 50 600 2638 2692 4176 4341 50
Size: 550 2038 54 1484 165 55
Fragments arranged by size:
2038 1484 550 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 835 2344 2525 2734 835
Size: 1509 181 209 2447
Fragments arranged by size:
2447 1509 209 181
BsrGI T'GTAC_A
Cuts at: 1085 1085
Size: 4346
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstEII G'GTnAC_C
Cuts at: 493 493
Size: 4346
BstYI r'GATC_y
Cuts at: 1 387 1029 1371 1838 1861 2196 2442 3454
Size: 386 642 342 467 23 335 246 1012
Cuts at: 3454 3540 3552 3898 1
Size: 86 12 346 449
Fragments arranged by size:
1012 642 467 449 386 346 342 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1035 1035
Size: 4346
BtgI C'CryG_G
Cuts at: 188 188
Size: 4346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 377 639 2296 2477 2640 377
Size: 262 1657 181 163 2083
Fragments arranged by size:
2083 1657 262 181 163
BtsI GCAGTG_nn'
Cuts at: 1589 1589
Size: 4346
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 520 2052 2910 520
Size: 1532 858 1956
Fragments arranged by size:
1956 1532 858
EaeI y'GGCC_r
Cuts at: 372 673 833 1173 1931 2105 2496 2523 3868
Size: 301 160 340 758 174 391 27 1345
Cuts at: 3868 372
Size: 850
Fragments arranged by size:
1345 850 758 391 340 301 174 160
27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 274 2369 2579 274
Size: 2095 210 2041
Fragments arranged by size:
2095 2041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 966 2554 2864 3010 4156 739
Size: 227 1588 310 146 1146 929
Fragments arranged by size:
1588 1146 929 310 227 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 469 1113 1268 1345 2171 2603 3350 3618
Size: 333 644 155 77 826 432 747 268
Cuts at: 3618 136
Size: 864
Fragments arranged by size:
864 826 747 644 432 333 268 155
77
EcoO109I rG'GnC_Cy
Cuts at: 240 1485 240
Size: 1245 3101
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FseI GG_CCGG'CC
Cuts at: 839 839
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 399 1118 1780 2028 3050 4029 399
Size: 719 662 248 1022 979 716
Fragments arranged by size:
1022 979 719 716 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 342 372 374 404 1207 1239 2188 2218 2220
Size: 30 2 30 803 32 949 30 2
Cuts at: 2220 2250 342
Size: 30 2438
Fragments arranged by size:
2438 949 803 32 30 30 30 30
2 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 615 1305 1366 615
Size: 690 61 3595
Fragments arranged by size:
3595 690 61
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 3017 3201 1127
Size: 1890 184 2272
Fragments arranged by size:
2272 1890 184
MscI TGG'CCA
Cuts at: 675 1175 2107 675
Size: 500 932 2914
Fragments arranged by size:
2914 932 500
MslI CAynn'nnrTG
Cuts at: 277 487 851 1081 2335 2462 277
Size: 210 364 230 1254 127 2161
Fragments arranged by size:
2161 1254 364 230 210 127
MspA1I CmG'CkG
Cuts at: 218 671 1133 1179 1457 1771 2131 3144 3389
Size: 453 462 46 278 314 360 1013 245
Cuts at: 3389 218
Size: 1175
Fragments arranged by size:
1175 1013 462 453 360 314 278 245
46
NaeI GCC'GGC
Cuts at: 837 2527 837
Size: 1690 2656
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NcoI C'CATG_G
Cuts at: 188 188
Size: 4346
NgoMIV G'CCGG_C
Cuts at: 835 2525 835
Size: 1690 2656
NheI G'CTAG_C
Cuts at: 1111 1111
Size: 4346
NruI TCG'CGA
Cuts at: 362 362
Size: 4346
NspI r_CATG'y
Cuts at: 142 564 867 1092 1399 1899 2806 142
Size: 422 303 225 307 500 907 1682
Fragments arranged by size:
1682 907 500 422 307 303 225
PasI CC'CwG_GG
Cuts at: 441 606 841 441
Size: 165 235 3946
Fragments arranged by size:
3946 235 165
PciI A'CATG_T
Cuts at: 138 2802 138
Size: 2664 1682
PflMI CCAn_nnn'nTGG
Cuts at: 194 987 194
Size: 793 3553
PfoI T'CCnGG_A
Cuts at: 524 761 524
Size: 237 4109
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 240 240
Size: 4346
PspOMI G'GGCC_C
Cuts at: 602 908 602
Size: 306 4040
PstI C_TGCA'G
Cuts at: 179 670 827 1001 1138 1393 2078 179
Size: 491 157 174 137 255 685 2447
Fragments arranged by size:
2447 685 491 255 174 157 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 671 1133 1179 1771 2131 671
Size: 462 46 592 360 2886
Fragments arranged by size:
2886 592 462 360 46
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 274 2369 2579 274
Size: 2095 210 2041
Fragments arranged by size:
2095 2041 210
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 175 666 711 823 997 1134 1389 2074
Size: 142 491 45 112 174 137 255 685
Cuts at: 2074 3067 3258 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 491 255 191 174 142
137 112 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 916 916
Size: 4346
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 907 1368 907
Size: 461 3885
SmlI C'TyrA_G
Cuts at: 565 1501 2416 2908 3170 565
Size: 936 915 492 262 1741
Fragments arranged by size:
1741 936 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StyI C'CwwG_G
Cuts at: 188 700 1170 1480 1834 188
Size: 512 470 310 354 2700
Fragments arranged by size:
2700 512 470 354 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 201 1156 1929 2062 2521 2730 201
Size: 955 773 133 459 209 1817
Fragments arranged by size:
1817 955 773 459 209 133
TatI w'GTAC_w
Cuts at: 1085 3861 1085
Size: 2776 1570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 757 789 3863 3895 757
Size: 32 3074 32 1208
Fragments arranged by size:
3074 1208 32 32
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 191 195 1168 1320 1841 191
Size: 4 973 152 521 2696
Fragments arranged by size:
2696 973 521 152 4
XmaI C'CCGG_G
Cuts at: 905 1366 905
Size: 461 3885
XmnI GAAnn'nnTTC
Cuts at: 799 799
Size: 4346
ZraI GAC'GTC
Cuts at: 1218 1218
Size: 4346
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BbvCI
BfrBI BmgBI Bpu10I BsgI BsiWI BsmBI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NotI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI