Restriction map for
pF1KE0533
(Circular) (Six-base) MAPSORT of: pF1KE0533.seq Check: 31 from: 1 to: 3731
pF1KE0533 3731 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 769 769
Size: 3731
Acc65I G'GTAC_C
Cuts at: 747 747
Size: 3731
AclI AA'CG_TT
Cuts at: 3571 3571
Size: 3731
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 692 1556 1988 2735 3003 481
Size: 211 864 432 747 268 1209
Fragments arranged by size:
1209 864 747 432 268 211
AfeI AGC'GCT
Cuts at: 307 307
Size: 3731
AflIII A'CryG_T
Cuts at: 1083 2187 3164 1083
Size: 1104 977 1650
Fragments arranged by size:
1650 1104 977
AgeI A'CCGG_T
Cuts at: 2119 2119
Size: 3731
AleI CACnn'nnGTG
Cuts at: 697 709 697
Size: 12 3719
AlwNI CAG_nnn'CTG
Cuts at: 1036 2603 1036
Size: 1567 2164
ApaLI G'TGCA_C
Cuts at: 2501 2501
Size: 3731
ApoI r'AATT_y
Cuts at: 735 3084 3340 735
Size: 2349 256 1126
Fragments arranged by size:
2349 1126 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3731
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
AvaI C'yCGr_G
Cuts at: 751 751
Size: 3731
BamHI G'GATC_C
Cuts at: 600 756 600
Size: 156 3575
BanI G'GyrC_C
Cuts at: 747 1161 1409 1444 747
Size: 414 248 35 3034
Fragments arranged by size:
3034 414 248 35
BanII G_rGCy'C
Cuts at: 425 453 745 1775 425
Size: 28 292 1030 2381
Fragments arranged by size:
2381 1030 292 28
BbeI G_GCGC'C
Cuts at: 1165 1413 1165
Size: 248 3483
BbvCI CC'TCA_GC
Cuts at: 584 584
Size: 3731
BciVI GTATCCnnnnn_n'
Cuts at: 1629 2390 3731 1629
Size: 761 1341 1629
Fragments arranged by size:
1629 1341 761
BclI T'GATC_A
Cuts at: 1251 1251
Size: 3731
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3731
BglII A'GATC_T
Cuts at: 1246 3283 1246
Size: 2037 1694
BlpI GC'TnA_GC
Cuts at: 843 843
Size: 3731
Bme1580I G_kGCm'C
Cuts at: 1356 1449 2505 1356
Size: 93 1056 2582
Fragments arranged by size:
2582 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 943 1024 1359 3503 943
Size: 81 335 2144 1171
Fragments arranged by size:
2144 1171 335 81
BmtI G_CTAG'C
Cuts at: 142 142
Size: 3731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 467 2098 467
Size: 1631 2100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 568
Size: 3731
Bpu10I CC'TnA_GC
Cuts at: 584 584
Size: 3731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 329 907 1786 2278 2576 329
Size: 578 879 492 298 1484
Fragments arranged by size:
1484 879 578 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3435 3435
Size: 3731
BsaAI yAC'GTr
Cuts at: 1084 1084
Size: 3731
BsaBI GATnn'nnATC
Cuts at: 6 1245 3282 6
Size: 1239 2037 455
Fragments arranged by size:
2037 1239 455
BsaHI Gr'CG_yC
Cuts at: 1162 1410 2112 3608 1162
Size: 248 702 1496 1285
Fragments arranged by size:
1496 1285 702 248
BsaWI w'CCGG_w
Cuts at: 920 1441 2119 2393 2540 2951 920
Size: 521 678 274 147 411 1700
Fragments arranged by size:
1700 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 459 448
Size: 11 3720
BseYI C'CCAG_C
Cuts at: 533 1156 1914 2491 2987 533
Size: 623 758 577 496 1277
Fragments arranged by size:
1277 758 623 577 496
BsiEI CG_ry'CG
Cuts at: 104 1273 1319 2527 104
Size: 1169 46 1208 1308
Fragments arranged by size:
1308 1208 1169 46
BsiHKAI G_wGCw'C
Cuts at: 425 453 745 1523 1713 2505 425
Size: 28 292 778 190 792 1651
Fragments arranged by size:
1651 792 778 292 190 28
Bsp1286I G_dGCh'C
Cuts at: 425 453 745 1356 1449 1523 1713 1775 2505
Size: 28 292 611 93 74 190 62 730
Cuts at: 2505 425
Size: 1651
Fragments arranged by size:
1651 730 611 292 190 93 74 62
28
BspEI T'CCGG_A
Cuts at: 2951 2951
Size: 3731
BspHI T'CATG_A
Cuts at: 2907 2907
Size: 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 668 781 995 1297 1678 668
Size: 113 214 302 381 2721
Fragments arranged by size:
2721 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 408 2023 2077 3561 3726 50
Size: 358 1615 54 1484 165 55
Fragments arranged by size:
1615 1484 358 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1643 1643
Size: 3731
BsrFI r'CCGG_y
Cuts at: 1729 1910 2119 1729
Size: 181 209 3341
Fragments arranged by size:
3341 209 181
BsrGI T'GTAC_A
Cuts at: 476 701 476
Size: 225 3506
BssSI C'ACGA_G
Cuts at: 2002 2360 2002
Size: 358 3373
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1108
Size: 3731
BstBI TT'CG_AA
Cuts at: 258 2092 258
Size: 1834 1897
BstYI r'GATC_y
Cuts at: 1 276 320 600 756 1223 1246 1581 1827
Size: 275 44 280 156 467 23 335 246
Cuts at: 1827 2839 2925 2937 3283 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 280 275 246
156 86 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 413 413
Size: 3731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1681 1862 2025 1681
Size: 181 163 3387
Fragments arranged by size:
3387 181 163
BtsI GCAGTG_nn'
Cuts at: 974 974
Size: 3731
DraI TTT'AAA
Cuts at: 730 730
Size: 3731
DrdI GACnn_nn'nnGTC
Cuts at: 1437 2295 1437
Size: 858 2873
EaeI y'GGCC_r
Cuts at: 1316 1490 1881 1908 3253 1316
Size: 174 391 27 1345 1794
Fragments arranged by size:
1794 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1316 1316
Size: 3731
EarI CTCTTCn'nnn_
Cuts at: 260 1754 1964 260
Size: 1494 210 2027
Fragments arranged by size:
2027 1494 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1939 2249 2395 3541 1939
Size: 310 146 1146 2129
Fragments arranged by size:
2129 1146 310 146
EcoICRI GAG'CTC
Cuts at: 423 451 743 423
Size: 28 292 3411
Fragments arranged by size:
3411 292 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 568 692 1556 1988 2735 3003 481
Size: 87 124 864 432 747 268 1209
Fragments arranged by size:
1209 864 747 432 268 124 87
EcoO109I rG'GnC_Cy
Cuts at: 870 870
Size: 3731
EcoRI G'AATT_C
Cuts at: 735 735
Size: 3731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 324 489 1750 324
Size: 165 1261 2305
Fragments arranged by size:
2305 1261 165
FspI TGC'GCA
Cuts at: 1512 1512
Size: 3731
HaeII r_GCGC'y
Cuts at: 309 1165 1413 2435 3414 309
Size: 856 248 1022 979 626
Fragments arranged by size:
1022 979 856 626 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1573 1603 1605 1635 1573
Size: 30 2 30 3669
Fragments arranged by size:
3669 30 30 2
HincII GTy'rAC
Cuts at: 770 770
Size: 3731
KasI G'GCGC_C
Cuts at: 1161 1409 1161
Size: 248 3483
KpnI G_GTAC'C
Cuts at: 751 751
Size: 3731
MluI A'CGCG_T
Cuts at: 3164 3164
Size: 3731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2402 2586 2402
Size: 184 3547
MscI TGG'CCA
Cuts at: 1492 1492
Size: 3731
MslI CAynn'nnrTG
Cuts at: 238 697 709 1720 1847 238
Size: 459 12 1011 127 2122
Fragments arranged by size:
2122 1011 459 127 12
MspA1I CmG'CkG
Cuts at: 842 1156 1516 2529 2774 842
Size: 314 360 1013 245 1799
Fragments arranged by size:
1799 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1912 1912
Size: 3731
NarI GG'CG_CC
Cuts at: 1162 1410 1162
Size: 248 3483
NgoMIV G'CCGG_C
Cuts at: 1910 1910
Size: 3731
NheI G'CTAG_C
Cuts at: 138 138
Size: 3731
NspI r_CATG'y
Cuts at: 784 1284 2191 784
Size: 500 907 2324
Fragments arranged by size:
2324 907 500
PciI A'CATG_T
Cuts at: 2187 2187
Size: 3731
PmeI GTTT'AAAC
Cuts at: 730 730
Size: 3731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 3699
PstI C_TGCA'G
Cuts at: 778 1463 778
Size: 685 3046
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3731
PvuII CAG'CTG
Cuts at: 1156 1516 1156
Size: 360 3371
RsrII CG'GwC_CG
Cuts at: 1926 1926
Size: 3731
SacI G_AGCT'C
Cuts at: 425 453 745 425
Size: 28 292 3411
Fragments arranged by size:
3411 292 28
SalI G'TCGA_C
Cuts at: 768 768
Size: 3731
SapI GCTCTTCn'nnn_
Cuts at: 1754 1964 1754
Size: 210 3521
SbfI CC_TGCA'GG
Cuts at: 778 778
Size: 3731
ScaI AGT'ACT
Cuts at: 3248 3248
Size: 3731
SfcI C'TryA_G
Cuts at: 33 774 1459 2452 2643 33
Size: 741 685 993 191 1121
Fragments arranged by size:
1121 993 741 685 191
SfoI GGC'GCC
Cuts at: 1163 1411 1163
Size: 248 3483
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3731
SmaI CCC'GGG
Cuts at: 753 753
Size: 3731
SmlI C'TyrA_G
Cuts at: 344 886 1801 2293 2555 344
Size: 542 915 492 262 1520
Fragments arranged by size:
1520 915 542 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3731
SphI G_CATG'C
Cuts at: 784 1284 784
Size: 500 3231
StyI C'CwwG_G
Cuts at: 695 865 1219 695
Size: 170 354 3207
Fragments arranged by size:
3207 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1314 1447 1906 2115 1314
Size: 133 459 209 2930
Fragments arranged by size:
2930 459 209 133
TatI w'GTAC_w
Cuts at: 476 701 3246 476
Size: 225 2545 961
Fragments arranged by size:
2545 961 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 635 667 3248 3280 635
Size: 32 2581 32 1086
Fragments arranged by size:
2581 1086 32 32
Tth111I GACn'n_nGTC
Cuts at: 1528 1528
Size: 3731
XbaI T'CTAG_A
Cuts at: 762 762
Size: 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1226 1226
Size: 3731
XmaI C'CCGG_G
Cuts at: 751 751
Size: 3731
XmnI GAAnn'nnTTC
Cuts at: 233 254 233
Size: 21 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI