Restriction map for
pF1KE0534
(Circular) (Six-base) MAPSORT of: pF1KE0534.seq Check: 7598 from: 1 to: 4436
pF1KE0534 4436 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1341 1341
Size: 4436
AccI GT'mk_AC
Cuts at: 527 1474 527
Size: 947 3489
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 978 2261 2693 3440 3708 708
Size: 270 1283 432 747 268 1436
Fragments arranged by size:
1436 1283 747 432 270 268
AflIII A'CryG_T
Cuts at: 431 1788 2892 3869 431
Size: 1357 1104 977 998
Fragments arranged by size:
1357 1104 998 977
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AlwNI CAG_nnn'CTG
Cuts at: 130 212 237 617 1333 1741 3308 130
Size: 82 25 380 716 408 1567 1258
Fragments arranged by size:
1567 1258 716 408 380 82 25
ApaLI G'TGCA_C
Cuts at: 3206 3206
Size: 4436
ApoI r'AATT_y
Cuts at: 1440 3789 4045 1440
Size: 2349 256 1831
Fragments arranged by size:
2349 1831 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 663 1456 663
Size: 793 3643
BamHI G'GATC_C
Cuts at: 1461 1461
Size: 4436
BanI G'GyrC_C
Cuts at: 223 635 1132 1452 1866 2114 2149 223
Size: 412 497 320 414 248 35 2510
Fragments arranged by size:
2510 497 414 412 320 248 35
BanII G_rGCy'C
Cuts at: 145 1109 1327 1450 2480 145
Size: 964 218 123 1030 2101
Fragments arranged by size:
2101 1030 964 218 123
BbeI G_GCGC'C
Cuts at: 227 1870 2118 227
Size: 1643 248 2545
Fragments arranged by size:
2545 1643 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 654 688 654
Size: 34 4402
BciVI GTATCCnnnnn_n'
Cuts at: 2334 3095 4436 2334
Size: 761 1341 2334
Fragments arranged by size:
2334 1341 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglI GCCn_nnn'nGGC
Cuts at: 666 1303 666
Size: 637 3799
BglII A'GATC_T
Cuts at: 623 1951 3988 623
Size: 1328 2037 1071
Fragments arranged by size:
2037 1328 1071
BlpI GC'TnA_GC
Cuts at: 1326 1548 1326
Size: 222 4214
Bme1580I G_kGCm'C
Cuts at: 115 640 662 1135 2061 2154 3210 115
Size: 525 22 473 926 93 1056 1341
Fragments arranged by size:
1341 1056 926 525 473 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 315 924 1138 1648 1729 2064 4208 315
Size: 609 214 510 81 335 2144 543
Fragments arranged by size:
2144 609 543 510 335 214 81
BmtI G_CTAG'C
Cuts at: 124 124
Size: 4436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 878 1236 648
Size: 230 358 3848
Fragments arranged by size:
3848 358 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1612 2491 2983 3281 1033
Size: 579 879 492 298 2188
Fragments arranged by size:
2188 879 579 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 754 4140 754
Size: 3386 1050
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 1950 3987 6
Size: 1944 2037 455
Fragments arranged by size:
2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 224 463 1867 2115 2817 4313 224
Size: 239 1404 248 702 1496 347
Fragments arranged by size:
1496 1404 702 347 248 239
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3098 3245 3656 1625
Size: 521 678 274 147 411 2405
Fragments arranged by size:
2405 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 416
Size: 30 4406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 1246 271
Size: 975 3461
BseYI C'CCAG_C
Cuts at: 164 521 885 1149 1354 1861 2619 3196 3692
Size: 357 364 264 205 507 758 577 496
Cuts at: 3692 164
Size: 908
Fragments arranged by size:
908 758 577 507 496 364 357 264
205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1319
Size: 4436
BsiEI CG_ry'CG
Cuts at: 104 1121 1978 2024 3232 104
Size: 1017 857 46 1208 1308
Fragments arranged by size:
1308 1208 1017 857 46
BsiHKAI G_wGCw'C
Cuts at: 497 1327 1450 2228 2418 3210 497
Size: 830 123 778 190 792 1723
Fragments arranged by size:
1723 830 792 778 190 123
Bsp1286I G_dGCh'C
Cuts at: 115 145 497 640 662 1109 1135 1327 1450
Size: 30 352 143 22 447 26 192 123
Cuts at: 1450 2061 2154 2228 2418 2480 3210 115
Size: 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 447 352 192 190 143
123 93 74 62 30 26 22
BspEI T'CCGG_A
Cuts at: 3656 3656
Size: 4436
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1184 1341 1486 1700 2002 2383 1184
Size: 157 145 214 302 381 3237
Fragments arranged by size:
3237 381 302 214 157 145
BsrBI CCG'CTC
Cuts at: 50 306 2728 2782 4266 4431 50
Size: 256 2422 54 1484 165 55
Fragments arranged by size:
2422 1484 256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2348 2348
Size: 4436
BsrFI r'CCGG_y
Cuts at: 383 2434 2615 2824 383
Size: 2051 181 209 1995
Fragments arranged by size:
2051 1995 209 181
BssSI C'ACGA_G
Cuts at: 844 2707 3065 844
Size: 1863 358 2215
Fragments arranged by size:
2215 1863 358
BstAPI GCAn_nnn'nTGC
Cuts at: 237 1813 237
Size: 1576 2860
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstEII G'GTnAC_C
Cuts at: 444 1158 444
Size: 714 3722
BstXI CCAn_nnnn'nTGG
Cuts at: 756 756
Size: 4436
BstYI r'GATC_y
Cuts at: 1 623 1461 1928 1951 2286 2532 3544 3630
Size: 622 838 467 23 335 246 1012 86
Cuts at: 3630 3642 3988 1
Size: 12 346 449
Fragments arranged by size:
1012 838 622 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 419 419
Size: 4436
BtgI C'CryG_G
Cuts at: 567 641 1115 1340 567
Size: 74 474 225 3663
Fragments arranged by size:
3663 474 225 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 786 1346 1679 786
Size: 560 333 3543
Fragments arranged by size:
3543 560 333
DraI TTT'AAA
Cuts at: 1435 1435
Size: 4436
DrdI GACnn_nn'nnGTC
Cuts at: 1078 2142 3000 1078
Size: 1064 858 2514
Fragments arranged by size:
2514 1064 858
EaeI y'GGCC_r
Cuts at: 398 848 1118 1304 1343 2021 2195 2586 2613
Size: 450 270 186 39 678 174 391 27
Cuts at: 2613 3958 398
Size: 1345 876
Fragments arranged by size:
1345 876 678 450 391 270 186 174
39 27
EagI C'GGCC_G
Cuts at: 1118 2021 1118
Size: 903 3533
EarI CTCTTCn'nnn_
Cuts at: 976 2459 2669 976
Size: 1483 210 2743
Fragments arranged by size:
2743 1483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2644 2954 3100 4246 2644
Size: 310 146 1146 2834
Fragments arranged by size:
2834 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1448 1325
Size: 123 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 708 878 978 1236 2261 2693 3440 3708
Size: 60 170 100 258 1025 432 747 268
Cuts at: 3708 648
Size: 1376
Fragments arranged by size:
1376 1025 747 432 268 258 170 100
60
EcoO109I rG'GnC_Cy
Cuts at: 309 605 614 668 768 840 863 873 1242
Size: 296 9 54 100 72 23 10 369
Cuts at: 1242 1575 309
Size: 333 3170
Fragments arranged by size:
3170 369 333 296 100 72 54 23
10 9
EcoRI G'AATT_C
Cuts at: 1440 1440
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 753 2455 753
Size: 1702 2734
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 227 280 1870 2118 3140 4119 227
Size: 53 1590 248 1022 979 544
Fragments arranged by size:
1590 1022 979 544 248 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 990 1022 2278 2308 2310 2340 484
Size: 32 474 32 1256 30 2 30 2580
Fragments arranged by size:
2580 1256 474 32 32 30 30 2
HincII GTy'rAC
Cuts at: 528 1475 528
Size: 947 3489
KasI G'GCGC_C
Cuts at: 223 1866 2114 223
Size: 1643 248 2545
Fragments arranged by size:
2545 1643 248
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3107 3291 3107
Size: 184 4252
MscI TGG'CCA
Cuts at: 850 2197 850
Size: 1347 3089
MslI CAynn'nnrTG
Cuts at: 1377 2425 2552 1377
Size: 1048 127 3261
Fragments arranged by size:
3261 1048 127
MspA1I CmG'CkG
Cuts at: 972 1547 1861 2221 3234 3479 972
Size: 575 314 360 1013 245 1929
Fragments arranged by size:
1929 1013 575 360 314 245
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 224 1867 2115 224
Size: 1643 248 2545
Fragments arranged by size:
2545 1643 248
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NheI G'CTAG_C
Cuts at: 120 120
Size: 4436
NspI r_CATG'y
Cuts at: 1489 1989 2896 1489
Size: 500 907 3029
Fragments arranged by size:
3029 907 500
PasI CC'CwG_GG
Cuts at: 148 148
Size: 4436
PciI A'CATG_T
Cuts at: 2892 2892
Size: 4436
PflMI CCAn_nnn'nTGG
Cuts at: 212 591 1010 212
Size: 379 419 3638
Fragments arranged by size:
3638 419 379
PfoI T'CCnGG_A
Cuts at: 1420 1420
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 450 3575 3607 418
Size: 32 3125 32 1247
Fragments arranged by size:
3125 1247 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 605 614 768 840 863 873 309
Size: 296 9 154 72 23 10 3872
Fragments arranged by size:
3872 296 154 72 23 10 9
PsiI TTA'TAA
Cuts at: 182 182
Size: 4436
PspXI vC'TCGA_Gb
Cuts at: 663 663
Size: 4436
PstI C_TGCA'G
Cuts at: 233 245 977 1483 2168 233
Size: 12 732 506 685 2501
Fragments arranged by size:
2501 732 685 506 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 972 1861 2221 972
Size: 889 360 3187
Fragments arranged by size:
3187 889 360
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1327 1450 1327
Size: 123 4313
SalI G'TCGA_C
Cuts at: 526 1473 526
Size: 947 3489
SanDI GG'GwC_CC
Cuts at: 605 768 605
Size: 163 4273
SapI GCTCTTCn'nnn_
Cuts at: 976 2459 2669 976
Size: 1483 210 2743
Fragments arranged by size:
2743 1483 210
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SfcI C'TryA_G
Cuts at: 33 229 241 896 973 1479 2164 3157 3348
Size: 196 12 655 77 506 685 993 191
Cuts at: 3348 33
Size: 1121
Fragments arranged by size:
1121 993 685 655 506 196 191 77
12
SfoI GGC'GCC
Cuts at: 225 1868 2116 225
Size: 1643 248 2545
Fragments arranged by size:
2545 1643 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 1458 1458
Size: 4436
SmlI C'TyrA_G
Cuts at: 663 1048 1591 2506 2998 3260 663
Size: 385 543 915 492 262 1839
Fragments arranged by size:
1839 915 543 492 385 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1489 1989 1489
Size: 500 3936
StyI C'CwwG_G
Cuts at: 755 851 1238 1570 1924 755
Size: 96 387 332 354 3267
Fragments arranged by size:
3267 387 354 332 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 877 1353 1417 2019 2152 2611 2820 877
Size: 476 64 602 133 459 209 2493
Fragments arranged by size:
2493 602 476 459 209 133 64
TatI w'GTAC_w
Cuts at: 3951 3951
Size: 4436
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 3953 3985 416
Size: 32 3505 32 867
Fragments arranged by size:
3505 867 32 32
Tth111I GACn'n_nGTC
Cuts at: 2233 2233
Size: 4436
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1931 1931
Size: 4436
XhoI C'TCGA_G
Cuts at: 663 663
Size: 4436
XmaI C'CCGG_G
Cuts at: 1456 1456
Size: 4436
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 4436
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PmlI PshAI
PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI