Restriction map for
pF1KE0536
(Circular) (Six-base) MAPSORT of: pF1KE0536.seq Check: 2464 from: 1 to: 3926
pF1KE0536 3926 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 277 964 277
Size: 687 3239
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 1231 2798 1231
Size: 1567 2359
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 124 287 703 930 3279 3535 124
Size: 163 416 227 2349 256 515
Fragments arranged by size:
2349 515 416 256 227 163
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 337 946 337
Size: 609 3317
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 942 1356 1604 1639 942
Size: 414 248 35 3229
Fragments arranged by size:
3229 414 248 35
BanII G_rGCy'C
Cuts at: 346 940 1970 346
Size: 594 1030 2302
Fragments arranged by size:
2302 1030 594
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbsI GAAGACnn'nnnn_
Cuts at: 587 836 854 587
Size: 249 18 3659
Fragments arranged by size:
3659 249 18
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 116 1446 116
Size: 1330 2596
BglII A'GATC_T
Cuts at: 1441 3478 1441
Size: 2037 1889
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 1551 1644 2700 1551
Size: 93 1056 2777
Fragments arranged by size:
2777 1056 93
BmgBI CAC'GTC
Cuts at: 905 905
Size: 3926
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 3926
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 1357 1605 2307 3803 1357
Size: 248 702 1496 1480
Fragments arranged by size:
1496 1480 702 248
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 585 1351 2109 2686 3182 585
Size: 766 758 577 496 1329
Fragments arranged by size:
1329 766 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 940 1718 1908 2700 940
Size: 778 190 792 2166
Fragments arranged by size:
2166 792 778 190
BsmI GAATG_Cn'
Cuts at: 316 378 316
Size: 62 3864
Bsp1286I G_dGCh'C
Cuts at: 346 940 1551 1644 1718 1908 1970 2700 346
Size: 594 611 93 74 190 62 730 1572
Fragments arranged by size:
1572 730 611 594 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 357 3102 357
Size: 2745 1181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 976 1190 1492 1873 976
Size: 214 302 381 3029
Fragments arranged by size:
3029 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2218 2272 3756 3921 50
Size: 2168 54 1484 165 55
Fragments arranged by size:
2168 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BsrGI T'GTAC_A
Cuts at: 480 480
Size: 3926
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 1303 1303
Size: 3926
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstEII G'GTnAC_C
Cuts at: 693 693
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 503 503
Size: 3926
BstYI r'GATC_y
Cuts at: 1 492 951 1418 1441 1776 2022 3034 3120
Size: 491 459 467 23 335 246 1012 86
Cuts at: 3120 3132 3478 1
Size: 12 346 449
Fragments arranged by size:
1012 491 467 459 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 278 278
Size: 3926
BtgI C'CryG_G
Cuts at: 107 107
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 766 1876 2057 2220 766
Size: 1110 181 163 2472
Fragments arranged by size:
2472 1110 181 163
BtsI GCAGTG_nn'
Cuts at: 1169 1169
Size: 3926
ClaI AT'CG_AT
Cuts at: 815 815
Size: 3926
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 1511 1685 2076 2103 3448 1511
Size: 174 391 27 1345 1989
Fragments arranged by size:
1989 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 938 938
Size: 3926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1751 2183 2930 3198 1751
Size: 432 747 268 2479
Fragments arranged by size:
2479 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 619 628 1065 619
Size: 9 437 3480
Fragments arranged by size:
3480 437 9
EcoRI G'AATT_C
Cuts at: 124 930 124
Size: 806 3120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3926
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 318 350 1768 1798 1800 1830 318
Size: 32 1418 30 2 30 2414
Fragments arranged by size:
2414 1418 32 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MfeI C'AATT_G
Cuts at: 395 395
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 778 2597 2781 778
Size: 1819 184 1923
Fragments arranged by size:
1923 1819 184
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 1037 1351 1711 2724 2969 1037
Size: 314 360 1013 245 1994
Fragments arranged by size:
1994 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NcoI C'CATG_G
Cuts at: 107 107
Size: 3926
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 979 1479 2386 979
Size: 500 907 2519
Fragments arranged by size:
2519 907 500
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PpuMI rG'GwC_Cy
Cuts at: 628 628
Size: 3926
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 873 905 873
Size: 32 3894
PstI C_TGCA'G
Cuts at: 973 1658 973
Size: 685 3241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 1351 1711 1351
Size: 360 3566
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 940 940
Size: 3926
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SanDI GG'GwC_CC
Cuts at: 628 628
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SfcI C'TryA_G
Cuts at: 33 387 969 1654 2647 2838 33
Size: 354 582 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 354 191
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StyI C'CwwG_G
Cuts at: 107 347 1060 1414 107
Size: 240 713 354 2619
Fragments arranged by size:
2619 713 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 480 3441 480
Size: 2961 965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 3894
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 957 957
Size: 3926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BssHII Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI