Restriction map for
pF1KE0538
(Circular) (Six-base) MAPSORT of: pF1KE0538.seq Check: 1488 from: 1 to: 3800
pF1KE0538 3800 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 454 838 454
Size: 384 3416
Acc65I G'GTAC_C
Cuts at: 598 816 598
Size: 218 3582
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1625 2057 2804 3072 687
Size: 938 432 747 268 1415
Fragments arranged by size:
1415 938 747 432 268
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 258 1105 2672 258
Size: 847 1567 1386
Fragments arranged by size:
1567 1386 847
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 694 820 694
Size: 126 3674
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 590 623 590
Size: 33 3767
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 598 816 1230 1478 1513 598
Size: 218 414 248 35 2885
Fragments arranged by size:
2885 414 248 218 35
BanII G_rGCy'C
Cuts at: 695 814 1844 695
Size: 119 1030 2651
Fragments arranged by size:
2651 1030 119
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BbsI GAAGACnn'nnnn_
Cuts at: 122 122
Size: 3800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 738 772 738
Size: 34 3766
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BfrBI ATG'CAT
Cuts at: 366 491 366
Size: 125 3675
BglII A'GATC_T
Cuts at: 1315 3352 1315
Size: 2037 1763
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 1425 1518 2574 1425
Size: 93 1056 2651
Fragments arranged by size:
2651 1056 93
BmgBI CAC'GTC
Cuts at: 729 729
Size: 3800
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 798 2167 798
Size: 1369 2431
Bpu10I CC'TnA_GC
Cuts at: 442 442
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1855 2347 2645 976
Size: 879 492 298 2131
Fragments arranged by size:
2131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 754 3504 754
Size: 2750 1050
BsaAI yAC'GTr
Cuts at: 266 1153 266
Size: 887 2913
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 1231 1479 2181 3677 1231
Size: 248 702 1496 1354
Fragments arranged by size:
1496 1354 702 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 736 787 736
Size: 51 3749
BseYI C'CCAG_C
Cuts at: 1225 1983 2560 3056 1225
Size: 758 577 496 1969
Fragments arranged by size:
1969 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1342 1388 2596 104
Size: 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 46
BsiHKAI G_wGCw'C
Cuts at: 448 695 814 1592 1782 2574 448
Size: 247 119 778 190 792 1674
Fragments arranged by size:
1674 792 778 247 190 119
BsiWI C'GTAC_G
Cuts at: 266 266
Size: 3800
BsmBI CGTCTCn'nnnn_
Cuts at: 588 588
Size: 3800
Bsp1286I G_dGCh'C
Cuts at: 448 695 814 1425 1518 1592 1782 1844 2574
Size: 247 119 611 93 74 190 62 730
Cuts at: 2574 448
Size: 1674
Fragments arranged by size:
1674 730 611 247 190 119 93 74
62
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1064 1366 1747 850
Size: 214 302 381 2903
Fragments arranged by size:
2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 351 2092 2146 3630 3795 50
Size: 301 1741 54 1484 165 55
Fragments arranged by size:
1741 1484 301 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1712 1712
Size: 3800
BsrFI r'CCGG_y
Cuts at: 1798 1979 2188 1798
Size: 181 209 3410
Fragments arranged by size:
3410 209 181
BsrGI T'GTAC_A
Cuts at: 281 281
Size: 3800
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstXI CCAn_nnnn'nTGG
Cuts at: 219 321 219
Size: 102 3698
BstYI r'GATC_y
Cuts at: 1 482 825 1292 1315 1650 1896 2908 2994
Size: 481 343 467 23 335 246 1012 86
Cuts at: 2994 3006 3352 1
Size: 12 346 449
Fragments arranged by size:
1012 481 467 449 346 343 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 455 455
Size: 3800
BtgI C'CryG_G
Cuts at: 107 296 107
Size: 189 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 206 1043 206
Size: 837 2963
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 500 1385 1559 1950 1977 3322 500
Size: 885 174 391 27 1345 978
Fragments arranged by size:
1345 978 885 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 622 713 1823 2033 622
Size: 91 1110 210 2389
Fragments arranged by size:
2389 1110 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 693 812 693
Size: 119 3681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1625 2057 2804 3072 687
Size: 938 432 747 268 1415
Fragments arranged by size:
1415 938 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 939 939
Size: 3800
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 1234 1482 2504 3483 1234
Size: 248 1022 979 1551
Fragments arranged by size:
1551 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 1642 1672 1674 1704 751
Size: 32 859 30 2 30 2847
Fragments arranged by size:
2847 859 32 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
HindIII A'AGCT_T
Cuts at: 540 540
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 602 820 602
Size: 218 3582
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 2471 2655 119
Size: 2352 184 1264
Fragments arranged by size:
2352 1264 184
MscI TGG'CCA
Cuts at: 502 1561 502
Size: 1059 2741
MslI CAynn'nnrTG
Cuts at: 319 403 496 526 1789 1916 319
Size: 84 93 30 1263 127 2203
Fragments arranged by size:
2203 1263 127 93 84 30
MspA1I CmG'CkG
Cuts at: 258 911 1225 1585 2598 2843 258
Size: 653 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 653 360 314 245
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 107 107
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NsiI A_TGCA'T
Cuts at: 368 493 368
Size: 125 3675
NspI r_CATG'y
Cuts at: 366 370 853 1353 2260 366
Size: 4 483 500 907 1906
Fragments arranged by size:
1906 907 500 483 4
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 258 1225 1585 258
Size: 967 360 2473
Fragments arranged by size:
2473 967 360
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 695 814 695
Size: 119 3681
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 622 1823 2033 622
Size: 1201 210 2389
Fragments arranged by size:
2389 1201 210
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SexAI A'CCwGG_T
Cuts at: 448 448
Size: 3800
SfcI C'TryA_G
Cuts at: 33 432 843 1528 2521 2712 33
Size: 399 411 685 993 191 1121
Fragments arranged by size:
1121 993 685 411 399 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 694 955 1870 2362 2624 694
Size: 261 915 492 262 1870
Fragments arranged by size:
1870 915 492 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 366 370 853 1353 366
Size: 4 483 500 2813
Fragments arranged by size:
2813 500 483 4
StyI C'CwwG_G
Cuts at: 107 144 934 1288 107
Size: 37 790 354 2619
Fragments arranged by size:
2619 790 354 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 281 3315 281
Size: 3034 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1295
Size: 3800
XhoI C'TCGA_G
Cuts at: 694 694
Size: 3800
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
XmnI GAAnn'nnTTC
Cuts at: 616 616
Size: 3800
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BglI BmtI BpmI BsaXI
BsgI BsmI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI