Restriction map for
pF1KE0539
(Circular) (Six-base) MAPSORT of: pF1KE0539.seq Check: 4781 from: 1 to: 4322
pF1KE0539 4322 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 764 764
Size: 4322
AccI GT'mk_AC
Cuts at: 287 1360 287
Size: 1073 3249
Acc65I G'GTAC_C
Cuts at: 720 1338 720
Size: 618 3704
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1303 2147 2579 3326 3594 540
Size: 763 844 432 747 268 1268
Fragments arranged by size:
1268 844 763 747 432 268
AflII C'TTAA_G
Cuts at: 1201 1201
Size: 4322
AflIII A'CryG_T
Cuts at: 1674 2778 3755 1674
Size: 1104 977 2241
Fragments arranged by size:
2241 1104 977
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AleI CACnn'nnGTG
Cuts at: 437 1076 437
Size: 639 3683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1174 1174
Size: 4322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1000 1032 1000
Size: 32 4290
AlwNI CAG_nnn'CTG
Cuts at: 1627 3194 1627
Size: 1567 2755
ApaI G_GGCC'C
Cuts at: 699 699
Size: 4322
ApaLI G'TGCA_C
Cuts at: 3092 3092
Size: 4322
ApoI r'AATT_y
Cuts at: 1326 3675 3931 1326
Size: 2349 256 1717
Fragments arranged by size:
2349 1717 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 395 1342 395
Size: 947 3375
BamHI G'GATC_C
Cuts at: 298 1347 298
Size: 1049 3273
BanI G'GyrC_C
Cuts at: 720 1338 1752 2000 2035 720
Size: 618 414 248 35 3007
Fragments arranged by size:
3007 618 414 248 35
BanII G_rGCy'C
Cuts at: 699 1185 1336 2366 699
Size: 486 151 1030 2655
Fragments arranged by size:
2655 1030 486 151
BbeI G_GCGC'C
Cuts at: 1756 2004 1756
Size: 248 4074
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 868 902 868
Size: 34 4288
BciVI GTATCCnnnnn_n'
Cuts at: 244 2220 2981 4322 244
Size: 1976 761 1341 244
Fragments arranged by size:
1976 1341 761 244
BclI T'GATC_A
Cuts at: 1001 1842 1001
Size: 841 3481
BglII A'GATC_T
Cuts at: 1837 3874 1837
Size: 2037 2285
BlpI GC'TnA_GC
Cuts at: 428 1434 428
Size: 1006 3316
Bme1580I G_kGCm'C
Cuts at: 699 1947 2040 3096 699
Size: 1248 93 1056 1925
Fragments arranged by size:
1925 1248 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 702 1534 1615 1950 4094 702
Size: 832 81 335 2144 930
Fragments arranged by size:
2144 930 832 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1207 565
Size: 642 3680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1498 2377 2869 3167 730
Size: 768 879 492 298 1885
Fragments arranged by size:
1885 879 768 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4026 4026
Size: 4322
BsaAI yAC'GTr
Cuts at: 384 1675 384
Size: 1291 3031
BsaBI GATnn'nnATC
Cuts at: 6 652 1836 3873 6
Size: 646 1184 2037 455
Fragments arranged by size:
2037 1184 646 455
BsaHI Gr'CG_yC
Cuts at: 761 1753 2001 2703 4199 761
Size: 992 248 702 1496 884
Fragments arranged by size:
1496 992 884 702 248
BsaWI w'CCGG_w
Cuts at: 404 1511 2032 2710 2984 3131 3542 404
Size: 1107 521 678 274 147 411 1184
Fragments arranged by size:
1184 1107 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 659 689 1000 1030 1204 1234 659
Size: 30 311 30 174 30 3747
Fragments arranged by size:
3747 311 174 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 4322
BseYI C'CCAG_C
Cuts at: 913 1747 2505 3082 3578 913
Size: 834 758 577 496 1657
Fragments arranged by size:
1657 834 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4322
BsiEI CG_ry'CG
Cuts at: 104 1864 1910 3118 104
Size: 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 434 1142 1336 2114 2304 3096 434
Size: 708 194 778 190 792 1660
Fragments arranged by size:
1660 792 778 708 194 190
BsmI GAATG_Cn'
Cuts at: 451 1267 451
Size: 816 3506
Bsp1286I G_dGCh'C
Cuts at: 434 699 1142 1185 1336 1947 2040 2114 2304
Size: 265 443 43 151 611 93 74 190
Cuts at: 2304 2366 3096 434
Size: 62 730 1660
Fragments arranged by size:
1660 730 611 443 265 190 151 93
74 62 43
BspEI T'CCGG_A
Cuts at: 404 3542 404
Size: 3138 1184
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 708 1372 1586 1888 2269 708
Size: 664 214 302 381 2761
Fragments arranged by size:
2761 664 381 302 214
BsrBI CCG'CTC
Cuts at: 50 130 2614 2668 4152 4317 50
Size: 80 2484 54 1484 165 55
Fragments arranged by size:
2484 1484 165 80 55 54
BsrDI GCAATG_nn'
Cuts at: 593 2234 593
Size: 1641 2681
BsrFI r'CCGG_y
Cuts at: 225 2320 2501 2710 225
Size: 2095 181 209 1837
Fragments arranged by size:
2095 1837 209 181
BssSI C'ACGA_G
Cuts at: 887 2593 2951 887
Size: 1706 358 2258
Fragments arranged by size:
2258 1706 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1699 1699
Size: 4322
BstBI TT'CG_AA
Cuts at: 2683 2683
Size: 4322
BstEII G'GTnAC_C
Cuts at: 771 798 771
Size: 27 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 739 739
Size: 4322
BstYI r'GATC_y
Cuts at: 1 298 653 1347 1814 1837 2172 2418 3430
Size: 297 355 694 467 23 335 246 1012
Cuts at: 3430 3516 3528 3874 1
Size: 86 12 346 449
Fragments arranged by size:
1012 694 467 449 355 346 335 297
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 206 206
Size: 4322
BtgI C'CryG_G
Cuts at: 356 1233 1250 356
Size: 877 17 3428
Fragments arranged by size:
3428 877 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2272 2453 2616 2272
Size: 181 163 3978
Fragments arranged by size:
3978 181 163
BtsI GCAGTG_nn'
Cuts at: 1565 1565
Size: 4322
DraI TTT'AAA
Cuts at: 1321 1321
Size: 4322
DraIII CAC_nnn'GTG
Cuts at: 276 276
Size: 4322
DrdI GACnn_nn'nnGTC
Cuts at: 2028 2886 2028
Size: 858 3464
EaeI y'GGCC_r
Cuts at: 677 969 1230 1236 1907 2081 2472 2499 3844
Size: 292 261 6 671 174 391 27 1345
Cuts at: 3844 677
Size: 1155
Fragments arranged by size:
1345 1155 671 391 292 261 174 27
6
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 190 2345 2555 190
Size: 2155 210 1957
Fragments arranged by size:
2155 1957 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2530 2840 2986 4132 2530
Size: 310 146 1146 2720
Fragments arranged by size:
2720 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1334 1334
Size: 4322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 565 1207 1303 2147 2579 3326 3594 540
Size: 25 642 96 844 432 747 268 1268
Fragments arranged by size:
1268 844 747 642 432 268 96 25
EcoNI CCTnn'n_nnAGG
Cuts at: 853 985 853
Size: 132 4190
EcoO109I rG'GnC_Cy
Cuts at: 143 1461 143
Size: 1318 3004
EcoRI G'AATT_C
Cuts at: 1326 1326
Size: 4322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 510 2341 438
Size: 72 1831 2419
Fragments arranged by size:
2419 1831 72
FspI TGC'GCA
Cuts at: 853 2103 853
Size: 1250 3072
HaeII r_GCGC'y
Cuts at: 1756 2004 3026 4005 1756
Size: 248 1022 979 2073
Fragments arranged by size:
2073 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1204 1236 2164 2194 2196 2226 202
Size: 32 970 32 928 30 2 30 2298
Fragments arranged by size:
2298 970 928 32 32 30 30 2
HincII GTy'rAC
Cuts at: 288 1361 288
Size: 1073 3249
KasI G'GCGC_C
Cuts at: 1752 2000 1752
Size: 248 4074
KpnI G_GTAC'C
Cuts at: 724 1342 724
Size: 618 3704
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2993 3177 2993
Size: 184 4138
MscI TGG'CCA
Cuts at: 971 1232 2083 971
Size: 261 851 3210
Fragments arranged by size:
3210 851 261
MslI CAynn'nnrTG
Cuts at: 437 607 769 1076 2311 2438 437
Size: 170 162 307 1235 127 2321
Fragments arranged by size:
2321 1235 307 170 162 127
MspA1I CmG'CkG
Cuts at: 543 1433 1747 2107 3120 3365 543
Size: 890 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 890 360 314 245
NaeI GCC'GGC
Cuts at: 2503 2503
Size: 4322
NarI GG'CG_CC
Cuts at: 1753 2001 1753
Size: 248 4074
NcoI C'CATG_G
Cuts at: 356 1233 1250 356
Size: 877 17 3428
Fragments arranged by size:
3428 877 17
NgoMIV G'CCGG_C
Cuts at: 2501 2501
Size: 4322
NspI r_CATG'y
Cuts at: 1375 1875 2782 1375
Size: 500 907 2915
Fragments arranged by size:
2915 907 500
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PflMI CCAn_nnn'nTGG
Cuts at: 648 809 1251 648
Size: 161 442 3719
Fragments arranged by size:
3719 442 161
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 3461 3493 1000
Size: 32 2429 32 1829
Fragments arranged by size:
2429 1829 32 32
PpuMI rG'GwC_Cy
Cuts at: 143 143
Size: 4322
PshAI GACnn'nnGTC
Cuts at: 141 141
Size: 4322
PsiI TTA'TAA
Cuts at: 962 962
Size: 4322
PspOMI G'GGCC_C
Cuts at: 695 695
Size: 4322
PspXI vC'TCGA_Gb
Cuts at: 395 395
Size: 4322
PstI C_TGCA'G
Cuts at: 1369 2054 1369
Size: 685 3637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 543 1747 2107 543
Size: 1204 360 2758
Fragments arranged by size:
2758 1204 360
RsrII CG'GwC_CG
Cuts at: 2517 2517
Size: 4322
SacI G_AGCT'C
Cuts at: 1336 1336
Size: 4322
SalI G'TCGA_C
Cuts at: 286 1359 286
Size: 1073 3249
SanDI GG'GwC_CC
Cuts at: 143 143
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 190 2345 2555 190
Size: 2155 210 1957
Fragments arranged by size:
2155 1957 210
SbfI CC_TGCA'GG
Cuts at: 1369 1369
Size: 4322
ScaI AGT'ACT
Cuts at: 505 574 3839 505
Size: 69 3265 988
Fragments arranged by size:
3265 988 69
SfcI C'TryA_G
Cuts at: 33 1365 2050 3043 3234 33
Size: 1332 685 993 191 1121
Fragments arranged by size:
1332 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1754 2002 1754
Size: 248 4074
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 395 709 1201 1477 2392 2884 3146 395
Size: 314 492 276 915 492 262 1571
Fragments arranged by size:
1571 915 492 492 314 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 1375 1875 1375
Size: 500 3822
StuI AGG'CCT
Cuts at: 401 493 848 401
Size: 92 355 3875
Fragments arranged by size:
3875 355 92
StyI C'CwwG_G
Cuts at: 302 356 776 1233 1250 1456 1810 302
Size: 54 420 457 17 206 354 2814
Fragments arranged by size:
2814 457 420 354 206 54 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 369 619 1048 1307 1905 2038 2497 2706 369
Size: 250 429 259 598 133 459 209 1985
Fragments arranged by size:
1985 598 459 429 259 250 209 133
TatI w'GTAC_w
Cuts at: 503 572 3837 503
Size: 69 3265 988
Fragments arranged by size:
3265 988 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 290 322 659 691 3839 3871 290
Size: 32 337 32 3148 32 741
Fragments arranged by size:
3148 741 337 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1096 2119 1096
Size: 1023 3299
XbaI T'CTAG_A
Cuts at: 1353 1353
Size: 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 1817 363
Size: 1454 2868
XhoI C'TCGA_G
Cuts at: 395 395
Size: 4322
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
XmnI GAAnn'nnTTC
Cuts at: 199 553 1267 199
Size: 354 714 3254
Fragments arranged by size:
3254 714 354
ZraI GAC'GTC
Cuts at: 762 762
Size: 4322
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AscI AvrII BaeI BbsI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI