Restriction map for
pF1KE0541
(Circular) (Six-base) MAPSORT of: pF1KE0541.seq Check: 324 from: 1 to: 3776
pF1KE0541 3776 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 814 814
Size: 3776
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1601 2033 2780 3048 253
Size: 1348 432 747 268 981
Fragments arranged by size:
1348 981 747 432 268
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 199 2164 199
Size: 1965 1811
AleI CACnn'nnGTG
Cuts at: 430 610 430
Size: 180 3596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 466 466
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 1081 2648 1081
Size: 1567 2209
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 796 796
Size: 3776
BamHI G'GATC_C
Cuts at: 289 356 801 289
Size: 67 445 3264
Fragments arranged by size:
3264 445 67
BanI G'GyrC_C
Cuts at: 187 380 604 792 1206 1454 1489 187
Size: 193 224 188 414 248 35 2474
Fragments arranged by size:
2474 414 248 224 193 188 35
BanII G_rGCy'C
Cuts at: 212 749 790 1820 212
Size: 537 41 1030 2168
Fragments arranged by size:
2168 1030 537 41
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbsI GAAGACnn'nnnn_
Cuts at: 518 518
Size: 3776
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 385 607 1401 1494 2550 385
Size: 222 794 93 1056 1611
Fragments arranged by size:
1611 1056 794 222 93
BmrI ACTGGGnnnn_n'
Cuts at: 576 677 988 1069 1404 3548 576
Size: 101 311 81 335 2144 804
Fragments arranged by size:
2144 804 335 311 101 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1831 2323 2621 952
Size: 879 492 298 2107
Fragments arranged by size:
2107 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3480 3480
Size: 3776
BsaAI yAC'GTr
Cuts at: 704 1129 704
Size: 425 3351
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 328 338 1207 1455 2157 3653 328
Size: 10 869 248 702 1496 451
Fragments arranged by size:
1496 869 702 451 248 10
BsaWI w'CCGG_w
Cuts at: 199 965 1486 2164 2438 2585 2996 199
Size: 766 521 678 274 147 411 979
Fragments arranged by size:
979 766 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 364 394 364
Size: 30 3746
BseYI C'CCAG_C
Cuts at: 1201 1959 2536 3032 1201
Size: 758 577 496 1945
Fragments arranged by size:
1945 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 436 250
Size: 186 3590
BsiEI CG_ry'CG
Cuts at: 104 1318 1364 2572 104
Size: 1214 46 1208 1308
Fragments arranged by size:
1308 1214 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 790 1568 1758 2550 212
Size: 578 778 190 792 1438
Fragments arranged by size:
1438 792 778 578 190
Bsp1286I G_dGCh'C
Cuts at: 212 385 607 749 790 1401 1494 1568 1758
Size: 173 222 142 41 611 93 74 190
Cuts at: 1758 1820 2550 212
Size: 62 730 1438
Fragments arranged by size:
1438 730 611 222 190 173 142 93
74 62 41
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 458 745 826 1040 1342 1723 226
Size: 232 287 81 214 302 381 2279
Fragments arranged by size:
2279 381 302 287 232 214 81
BsrBI CCG'CTC
Cuts at: 50 2068 2122 3606 3771 50
Size: 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 141 199 316 1774 1955 2164 141
Size: 58 117 1458 181 209 1753
Fragments arranged by size:
1753 1458 209 181 117 58
BssSI C'ACGA_G
Cuts at: 211 2047 2405 211
Size: 1836 358 1582
Fragments arranged by size:
1836 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1153
Size: 3776
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstEII G'GTnAC_C
Cuts at: 312 312
Size: 3776
BstYI r'GATC_y
Cuts at: 1 289 356 801 1268 1291 1626 1872 2884
Size: 288 67 445 467 23 335 246 1012
Cuts at: 2884 2970 2982 3328 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 288 246
86 67 23 12
BtgI C'CryG_G
Cuts at: 123 360 654 672 713 123
Size: 237 294 18 41 3186
Fragments arranged by size:
3186 294 237 41 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1019
Size: 3776
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DraIII CAC_nnn'GTG
Cuts at: 258 258
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 222 1482 2340 222
Size: 1260 858 1658
Fragments arranged by size:
1658 1260 858
EaeI y'GGCC_r
Cuts at: 139 300 1361 1535 1926 1953 3298 139
Size: 161 1061 174 391 27 1345 617
Fragments arranged by size:
1345 1061 617 391 174 161 27
EagI C'GGCC_G
Cuts at: 1361 1361
Size: 3776
EarI CTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 369 1984 2294 2440 3586 369
Size: 1615 310 146 1146 559
Fragments arranged by size:
1615 1146 559 310 146
EcoICRI GAG'CTC
Cuts at: 210 788 210
Size: 578 3198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1601 2033 2780 3048 253
Size: 1348 432 747 268 981
Fragments arranged by size:
1348 981 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 392 915 392
Size: 523 3253
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 169 1210 1458 2480 3459 169
Size: 1041 248 1022 979 486
Fragments arranged by size:
1041 1022 979 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 815 815
Size: 3776
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 506 2447 2631 311
Size: 195 1941 184 1456
Fragments arranged by size:
1941 1456 195 184
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 430 610 1765 1892 430
Size: 180 1155 127 2314
Fragments arranged by size:
2314 1155 180 127
MspA1I CmG'CkG
Cuts at: 125 299 362 477 887 1201 1561 2574 2819
Size: 174 63 115 410 314 360 1013 245
Cuts at: 2819 125
Size: 1082
Fragments arranged by size:
1082 1013 410 360 314 245 174 115
63
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NcoI C'CATG_G
Cuts at: 713 713
Size: 3776
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 433 601 681 829 1329 2236 433
Size: 168 80 148 500 907 1973
Fragments arranged by size:
1973 907 500 168 148 80
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PflMI CCAn_nnn'nTGG
Cuts at: 600 600
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PpuMI rG'GwC_Cy
Cuts at: 392 392
Size: 3776
PstI C_TGCA'G
Cuts at: 756 823 1508 756
Size: 67 685 3024
Fragments arranged by size:
3024 685 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 1201 1561 1201
Size: 360 3416
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 212 790 212
Size: 578 3198
SacII CC_GC'GG
Cuts at: 126 363 126
Size: 237 3539
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SanDI GG'GwC_CC
Cuts at: 392 392
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 752 819 1504 2497 2688 33
Size: 719 67 685 993 191 1121
Fragments arranged by size:
1121 993 719 685 191 67
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 931 1846 2338 2600 931
Size: 915 492 262 2107
Fragments arranged by size:
2107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 681 829 1329 681
Size: 148 500 3128
Fragments arranged by size:
3128 500 148
StyI C'CwwG_G
Cuts at: 713 910 1264 713
Size: 197 354 3225
Fragments arranged by size:
3225 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 1492 1951 2160 1359
Size: 133 459 209 2975
Fragments arranged by size:
2975 459 209 133
TatI w'GTAC_w
Cuts at: 3291 3291
Size: 3776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 3293 3325 364
Size: 32 2897 32 815
Fragments arranged by size:
2897 815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI