Restriction map for
pF1KE0542
(Circular) (Six-base) MAPSORT of: pF1KE0542.seq Check: 1053 from: 1 to: 4034
pF1KE0542 4034 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 514 1030 1859 2291 3038 3306 202
Size: 312 516 829 432 747 268 930
Fragments arranged by size:
930 829 747 516 432 312 268
AflIII A'CryG_T
Cuts at: 1026 1386 2490 3467 1026
Size: 360 1104 977 1593
Fragments arranged by size:
1593 1104 977 360
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AleI CACnn'nnGTG
Cuts at: 760 760
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 332 1339 2906 332
Size: 1007 1567 1460
Fragments arranged by size:
1567 1460 1007
ApaLI G'TGCA_C
Cuts at: 1016 2804 1016
Size: 1788 2246
ApoI r'AATT_y
Cuts at: 511 1038 3387 3643 511
Size: 527 2349 256 902
Fragments arranged by size:
2349 902 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 534 567 534
Size: 33 4001
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 294 1050 1464 1712 1747 294
Size: 756 414 248 35 2581
Fragments arranged by size:
2581 756 414 248 35
BanII G_rGCy'C
Cuts at: 1048 2078 1048
Size: 1030 3004
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 530 627 838 530
Size: 97 211 3726
Fragments arranged by size:
3726 211 97
BciVI GTATCCnnnnn_n'
Cuts at: 192 1932 2693 4034 192
Size: 1740 761 1341 192
Fragments arranged by size:
1740 1341 761 192
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 417 1549 3586 417
Size: 1132 2037 865
Fragments arranged by size:
2037 1132 865
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 1020 1659 1752 2808 1020
Size: 639 93 1056 2246
Fragments arranged by size:
2246 1056 639 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2089 2581 2879 1210
Size: 879 492 298 2365
Fragments arranged by size:
2365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3738 3738
Size: 4034
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 807 1465 1713 2415 3911 807
Size: 658 248 702 1496 930
Fragments arranged by size:
1496 930 702 658 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1459 2217 2794 3290 1459
Size: 758 577 496 2203
Fragments arranged by size:
2203 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 755
Size: 4034
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 263 1020 1048 1826 2016 2808 263
Size: 757 28 778 190 792 1489
Fragments arranged by size:
1489 792 778 757 190 28
Bsp1286I G_dGCh'C
Cuts at: 263 1020 1048 1659 1752 1826 2016 2078 2808
Size: 757 28 611 93 74 190 62 730
Cuts at: 2808 263
Size: 1489
Fragments arranged by size:
1489 757 730 611 190 93 74 62
28
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 3210 3210
Size: 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1084 1298 1600 1981 1084
Size: 214 302 381 3137
Fragments arranged by size:
3137 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 182 622 1946 182
Size: 440 1324 2270
Fragments arranged by size:
2270 1324 440
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstEII G'GTnAC_C
Cuts at: 762 762
Size: 4034
BstYI r'GATC_y
Cuts at: 1 417 1059 1526 1549 1884 2130 3142 3228
Size: 416 642 467 23 335 246 1012 86
Cuts at: 3228 3240 3586 1
Size: 12 346 449
Fragments arranged by size:
1012 642 467 449 416 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 146 158 758 107
Size: 39 12 600 3383
Fragments arranged by size:
3383 600 39 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 596 1984 2165 2328 596
Size: 1388 181 163 2302
Fragments arranged by size:
2302 1388 181 163
BtsI GCAGTG_nn'
Cuts at: 944 1277 944
Size: 333 3701
DraI TTT'AAA
Cuts at: 674 689 1033 674
Size: 15 344 3675
Fragments arranged by size:
3675 344 15
DraIII CAC_nnn'GTG
Cuts at: 147 258 147
Size: 111 3923
DrdI GACnn_nn'nnGTC
Cuts at: 786 1740 2598 786
Size: 954 858 2222
Fragments arranged by size:
2222 954 858
EaeI y'GGCC_r
Cuts at: 149 1619 1793 2184 2211 3556 149
Size: 1470 174 391 27 1345 627
Fragments arranged by size:
1470 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1046
Size: 4034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 286 514 1030 1859 2291 3038 3306 202
Size: 84 228 516 829 432 747 268 930
Fragments arranged by size:
930 829 747 516 432 268 228 84
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 1876 1906 1908 1938 312
Size: 32 1532 30 2 30 2408
Fragments arranged by size:
2408 1532 32 30 30 2
HincII GTy'rAC
Cuts at: 397 1073 397
Size: 676 3358
HindIII A'AGCT_T
Cuts at: 496 703 496
Size: 207 3827
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 1795 1795
Size: 4034
MslI CAynn'nnrTG
Cuts at: 760 1000 2023 2150 760
Size: 240 1023 127 2644
Fragments arranged by size:
2644 1023 240 127
MspA1I CmG'CkG
Cuts at: 985 1145 1459 1819 2832 3077 985
Size: 160 314 360 1013 245 1942
Fragments arranged by size:
1942 1013 360 314 245 160
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 107 107
Size: 4034
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NruI TCG'CGA
Cuts at: 581 581
Size: 4034
NspI r_CATG'y
Cuts at: 1087 1587 2494 1087
Size: 500 907 2627
Fragments arranged by size:
2627 907 500
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PflMI CCAn_nnn'nTGG
Cuts at: 147 147
Size: 4034
PfoI T'CCnGG_A
Cuts at: 263 263
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 779 935 1081 1766 779
Size: 156 146 685 3047
Fragments arranged by size:
3047 685 156 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 985 1459 1819 985
Size: 474 360 3200
Fragments arranged by size:
3200 474 360
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 1048 1048
Size: 4034
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 775 931 1077 1762 2755 2946 33
Size: 742 156 146 685 993 191 1121
Fragments arranged by size:
1121 993 742 685 191 156 146
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 1189 2104 2596 2858 1189
Size: 915 492 262 2365
Fragments arranged by size:
2365 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
SspI AAT'ATT
Cuts at: 855 855
Size: 4034
StuI AGG'CCT
Cuts at: 914 914
Size: 4034
StyI C'CwwG_G
Cuts at: 107 713 1168 1522 107
Size: 606 455 354 2619
Fragments arranged by size:
2619 606 455 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 465 1617 1750 2209 2418 465
Size: 1152 133 459 209 2081
Fragments arranged by size:
2081 1152 459 209 133
TatI w'GTAC_w
Cuts at: 783 3549 783
Size: 2766 1268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 305 1529 305
Size: 1224 2810
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI