Restriction map for
pF1KE0543
(Circular) (Six-base) MAPSORT of: pF1KE0543.seq Check: 6321 from: 1 to: 4058
pF1KE0543 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1883 2315 3062 3330 433
Size: 1450 432 747 268 1161
Fragments arranged by size:
1450 1161 747 432 268
AflIII A'CryG_T
Cuts at: 884 1410 2514 3491 884
Size: 526 1104 977 1451
Fragments arranged by size:
1451 1104 977 526
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 123 642 1363 2930 123
Size: 519 721 1567 1251
Fragments arranged by size:
1567 1251 721 519
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 1062 3411 3667 1062
Size: 2349 256 1453
Fragments arranged by size:
2349 1453 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 275 1078 275
Size: 803 3255
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 593 667 1074 1488 1736 1771 593
Size: 74 407 414 248 35 2880
Fragments arranged by size:
2880 414 407 248 74 35
BanII G_rGCy'C
Cuts at: 167 535 653 752 1072 2102 167
Size: 368 118 99 320 1030 2123
Fragments arranged by size:
2123 1030 368 320 118 99
BbeI G_GCGC'C
Cuts at: 597 1492 1740 597
Size: 895 248 2915
Fragments arranged by size:
2915 895 248
BbsI GAAGACnn'nnnn_
Cuts at: 274 274
Size: 4058
BbvCI CC'TCA_GC
Cuts at: 540 540
Size: 4058
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglI GCCn_nnn'nGGC
Cuts at: 760 760
Size: 4058
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 972 1683 1776 2832 972
Size: 711 93 1056 2198
Fragments arranged by size:
2198 1056 711 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 540 320
Size: 220 3838
Bpu10I CC'TnA_GC
Cuts at: 540 540
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 847 986 1234 2113 2605 2903 469
Size: 378 139 248 879 492 298 1624
Fragments arranged by size:
1624 879 492 378 298 248 139
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 510 594 763 826 1489 1737 2439 3935 510
Size: 84 169 63 663 248 702 1496 633
Fragments arranged by size:
1496 702 663 633 248 169 84 63
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 4058
BseYI C'CCAG_C
Cuts at: 442 646 1483 2241 2818 3314 442
Size: 204 837 758 577 496 1186
Fragments arranged by size:
1186 837 758 577 496 204
BsiEI CG_ry'CG
Cuts at: 104 818 1600 1646 2854 104
Size: 714 782 46 1208 1308
Fragments arranged by size:
1308 1208 782 714 46
BsiHKAI G_wGCw'C
Cuts at: 752 1072 1850 2040 2832 752
Size: 320 778 190 792 1978
Fragments arranged by size:
1978 792 778 320 190
Bsp1286I G_dGCh'C
Cuts at: 167 535 653 752 972 1072 1683 1776 1850
Size: 368 118 99 220 100 611 93 74
Cuts at: 1850 2040 2102 2832 167
Size: 190 62 730 1393
Fragments arranged by size:
1393 730 611 368 220 190 118 100
99 93 74 62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 947 3234 947
Size: 2287 1771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 790 1108 1322 1624 2005 187
Size: 603 318 214 302 381 2240
Fragments arranged by size:
2240 603 381 318 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 611 1970 611
Size: 1359 2699
BsrFI r'CCGG_y
Cuts at: 754 2056 2237 2446 754
Size: 1302 181 209 2366
Fragments arranged by size:
2366 1302 209 181
BsrGI T'GTAC_A
Cuts at: 239 887 239
Size: 648 3410
BssSI C'ACGA_G
Cuts at: 166 2329 2687 166
Size: 2163 358 1537
Fragments arranged by size:
2163 1537 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstEII G'GTnAC_C
Cuts at: 174 462 174
Size: 288 3770
BstXI CCAn_nnnn'nTGG
Cuts at: 198 354 198
Size: 156 3902
BstYI r'GATC_y
Cuts at: 1 120 1083 1550 1573 1908 2154 3166 3252
Size: 119 963 467 23 335 246 1012 86
Cuts at: 3252 3264 3610 1
Size: 12 346 449
Fragments arranged by size:
1012 963 467 449 346 335 246 119
86 23 12
BtgI C'CryG_G
Cuts at: 473 473
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 320 827 1301 320
Size: 507 474 3077
Fragments arranged by size:
3077 507 474
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 594 629 594
Size: 35 4023
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 200 359 515 1643 1817 2208 2235 3580 200
Size: 159 156 1128 174 391 27 1345 678
Fragments arranged by size:
1345 1128 678 391 174 159 156 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 2266 2576 2722 3868 295
Size: 1971 310 146 1146 485
Fragments arranged by size:
1971 1146 485 310 146
EcoICRI GAG'CTC
Cuts at: 750 1070 750
Size: 320 3738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 433 540 1883 2315 3062 3330 320
Size: 113 107 1343 432 747 268 1048
Fragments arranged by size:
1343 1048 747 432 268 113 107
EcoNI CCTnn'n_nnAGG
Cuts at: 877 877
Size: 4058
EcoO109I rG'GnC_Cy
Cuts at: 560 639 660 1197 560
Size: 79 21 537 3421
Fragments arranged by size:
3421 537 79 21
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 783 2077 783
Size: 1294 2764
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 597 959 1492 1740 2762 3741 597
Size: 362 533 248 1022 979 914
Fragments arranged by size:
1022 979 914 533 362 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 922 954 1019 1051 1900 1930 1932 1962 922
Size: 32 65 32 849 30 2 30 3018
Fragments arranged by size:
3018 849 65 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 593 1488 1736 593
Size: 895 248 2915
Fragments arranged by size:
2915 895 248
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2729 2913 2729
Size: 184 3874
MscI TGG'CCA
Cuts at: 202 1819 202
Size: 1617 2441
MslI CAynn'nnrTG
Cuts at: 478 2047 2174 478
Size: 1569 127 2362
Fragments arranged by size:
2362 1569 127
MspA1I CmG'CkG
Cuts at: 405 495 1169 1483 1843 2856 3101 405
Size: 90 674 314 360 1013 245 1362
Fragments arranged by size:
1362 1013 674 360 314 245 90
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 594 1489 1737 594
Size: 895 248 2915
Fragments arranged by size:
2915 895 248
NcoI C'CATG_G
Cuts at: 473 473
Size: 4058
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 300 888 1111 1611 2518 300
Size: 588 223 500 907 1840
Fragments arranged by size:
1840 907 588 500 223
PciI A'CATG_T
Cuts at: 884 2514 884
Size: 1630 2428
PflMI CCAn_nnn'nTGG
Cuts at: 459 459
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PpuMI rG'GwC_Cy
Cuts at: 560 560
Size: 4058
PstI C_TGCA'G
Cuts at: 218 315 500 1105 1790 218
Size: 97 185 605 685 2486
Fragments arranged by size:
2486 685 605 185 97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 495 1483 1843 495
Size: 988 360 2710
Fragments arranged by size:
2710 988 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 752 1072 752
Size: 320 3738
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 214 311 496 654 1101 1786 2779 2970
Size: 181 97 185 158 447 685 993 191
Cuts at: 2970 33
Size: 1121
Fragments arranged by size:
1121 993 685 447 191 185 181 158
97
SfoI GGC'GCC
Cuts at: 595 1490 1738 595
Size: 895 248 2915
Fragments arranged by size:
2915 895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 484 862 1001 1213 2128 2620 2882 484
Size: 378 139 212 915 492 262 1660
Fragments arranged by size:
1660 915 492 378 262 212 139
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
StuI AGG'CCT
Cuts at: 872 872
Size: 4058
StyI C'CwwG_G
Cuts at: 473 1192 1546 473
Size: 719 354 2985
Fragments arranged by size:
2985 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 655 1641 1774 2233 2442 601
Size: 54 986 133 459 209 2217
Fragments arranged by size:
2217 986 459 209 133 54
TatI w'GTAC_w
Cuts at: 239 887 3573 239
Size: 648 2686 724
Fragments arranged by size:
2686 724 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4026
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 831 1089 831
Size: 258 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1553 1553
Size: 4058
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI