Restriction map for
pF1KE0547
(Circular) (Six-base) MAPSORT of: pF1KE0547.seq Check: 1844 from: 1 to: 4382
pF1KE0547 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1087 2207 2639 3386 3654 1006
Size: 81 1120 432 747 268 1734
Fragments arranged by size:
1734 1120 747 432 268 81
AflIII A'CryG_T
Cuts at: 743 812 1734 2838 3815 743
Size: 69 922 1104 977 1310
Fragments arranged by size:
1310 1104 977 922 69
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 799 831 799
Size: 32 4350
AlwNI CAG_nnn'CTG
Cuts at: 509 984 1317 1687 3254 509
Size: 475 333 370 1567 1637
Fragments arranged by size:
1637 1567 475 370 333
ApaI G_GGCC'C
Cuts at: 1325 1325
Size: 4382
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 210 1386 3735 3991 210
Size: 1176 2349 256 601
Fragments arranged by size:
2349 1176 601 256
AseI AT'TA_AT
Cuts at: 20 1151 20
Size: 1131 3251
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 590 789 1347 1402 590
Size: 199 558 55 3570
Fragments arranged by size:
3570 558 199 55
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 996 1398 1812 2060 2095 996
Size: 402 414 248 35 3283
Fragments arranged by size:
3283 414 402 248 35
BanII G_rGCy'C
Cuts at: 499 1325 1396 2426 499
Size: 826 71 1030 2455
Fragments arranged by size:
2455 1030 826 71
BbeI G_GCGC'C
Cuts at: 1000 1816 2064 1000
Size: 816 248 3318
Fragments arranged by size:
3318 816 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 933 967 1072 1106 1319 1353 933
Size: 34 105 34 213 34 3962
Fragments arranged by size:
3962 213 105 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 155 1902 155
Size: 1747 2635
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 1325 2007 2100 3156 1325
Size: 682 93 1056 2551
Fragments arranged by size:
2551 1056 682 93
BmgBI CAC'GTC
Cuts at: 1109 1109
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1378 2749 1378
Size: 1371 3011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 934 391
Size: 543 3839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1558 2437 2929 3227 1120
Size: 438 879 492 298 2275
Fragments arranged by size:
2275 879 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 4086 4086
Size: 4382
BsaAI yAC'GTr
Cuts at: 642 722 1735 642
Size: 80 1013 3289
Fragments arranged by size:
3289 1013 80
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 997 1813 2061 2763 4259 997
Size: 816 248 702 1496 1120
Fragments arranged by size:
1496 1120 816 702 248
BsaWI w'CCGG_w
Cuts at: 488 1571 2092 2770 3044 3191 3602 488
Size: 1083 521 678 274 147 411 1268
Fragments arranged by size:
1268 1083 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 496
Size: 4382
BseYI C'CCAG_C
Cuts at: 1807 2565 3142 3638 1807
Size: 758 577 496 2551
Fragments arranged by size:
2551 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 997 756
Size: 241 4141
BsiEI CG_ry'CG
Cuts at: 104 1339 1924 1970 3178 104
Size: 1235 585 46 1208 1308
Fragments arranged by size:
1308 1235 1208 585 46
BsiHKAI G_wGCw'C
Cuts at: 922 1396 2174 2364 3156 922
Size: 474 778 190 792 2148
Fragments arranged by size:
2148 792 778 474 190
BsmI GAATG_Cn'
Cuts at: 1036 1095 1036
Size: 59 4323
BsmBI CGTCTCn'nnnn_
Cuts at: 1106 1106
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 499 922 1325 1396 2007 2100 2174 2364 2426
Size: 423 403 71 611 93 74 190 62
Cuts at: 2426 3156 499
Size: 730 1725
Fragments arranged by size:
1725 730 611 423 403 190 93 74
71 62
BspEI T'CCGG_A
Cuts at: 488 3602 488
Size: 3114 1268
BspHI T'CATG_A
Cuts at: 515 3558 515
Size: 3043 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 704 908 1432 1646 1948 2329 704
Size: 204 524 214 302 381 2757
Fragments arranged by size:
2757 524 381 302 214 204
BsrBI CCG'CTC
Cuts at: 50 1357 2674 2728 4212 4377 50
Size: 1307 1317 54 1484 165 55
Fragments arranged by size:
1484 1317 1307 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 764 999 2380 2561 2770 764
Size: 235 1381 181 209 2376
Fragments arranged by size:
2376 1381 235 209 181
BsrGI T'GTAC_A
Cuts at: 746 746
Size: 4382
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 804 874 804
Size: 70 4312
BstYI r'GATC_y
Cuts at: 1 959 1407 1874 1897 2232 2478 3490 3576
Size: 958 448 467 23 335 246 1012 86
Cuts at: 3576 3588 3934 1
Size: 12 346 449
Fragments arranged by size:
1012 958 467 449 448 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1220 1220
Size: 4382
BtgI C'CryG_G
Cuts at: 107 873 924 107
Size: 766 51 3565
Fragments arranged by size:
3565 766 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1152 2332 2513 2676 1152
Size: 1180 181 163 2858
Fragments arranged by size:
2858 1180 181 163
BtsI GCAGTG_nn'
Cuts at: 730 1020 1115 1625 730
Size: 290 95 510 3487
Fragments arranged by size:
3487 510 290 95
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 289 2088 2946 289
Size: 1799 858 1725
Fragments arranged by size:
1799 1725 858
EaeI y'GGCC_r
Cuts at: 1967 2141 2532 2559 3904 1967
Size: 174 391 27 1345 2445
Fragments arranged by size:
2445 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 487 2405 2615 487
Size: 1918 210 2254
Fragments arranged by size:
2254 1918 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 934 1006 1087 2207 2639 3386 3654 391
Size: 543 72 81 1120 432 747 268 1119
Fragments arranged by size:
1120 1119 747 543 432 268 81 72
EcoO109I rG'GnC_Cy
Cuts at: 330 1321 1521 330
Size: 991 200 3191
Fragments arranged by size:
3191 991 200
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
EcoRV GAT'ATC
Cuts at: 138 268 138
Size: 130 4252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 567 1041 2401 567
Size: 474 1360 2548
Fragments arranged by size:
2548 1360 474
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1000 1816 2064 3086 4065 1000
Size: 816 248 1022 979 1317
Fragments arranged by size:
1317 1022 979 816 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 167 199 257 289 1287 1319 2224 2254 2256
Size: 32 58 32 998 32 905 30 2
Cuts at: 2256 2286 167
Size: 30 2263
Fragments arranged by size:
2263 998 905 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 294 1421 294
Size: 1127 3255
HindIII A'AGCT_T
Cuts at: 751 751
Size: 4382
KasI G'GCGC_C
Cuts at: 996 1812 2060 996
Size: 816 248 3318
Fragments arranged by size:
3318 816 248
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 448 872 2371 2498 448
Size: 424 1499 127 2332
Fragments arranged by size:
2332 1499 424 127
MspA1I CmG'CkG
Cuts at: 460 926 1493 1807 2167 3180 3425 460
Size: 466 567 314 360 1013 245 1417
Fragments arranged by size:
1417 1013 567 466 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 997 1813 2061 997
Size: 816 248 3318
Fragments arranged by size:
3318 816 248
NcoI C'CATG_G
Cuts at: 107 873 107
Size: 766 3616
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 747 816 1435 1935 2842 747
Size: 69 619 500 907 2287
Fragments arranged by size:
2287 907 619 500 69
PasI CC'CwG_GG
Cuts at: 537 1269 537
Size: 732 3650
PciI A'CATG_T
Cuts at: 743 812 2838 743
Size: 69 2026 2287
Fragments arranged by size:
2287 2026 69
PflMI CCAn_nnn'nTGG
Cuts at: 509 509
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 330 330
Size: 4382
PspOMI G'GGCC_C
Cuts at: 1321 1321
Size: 4382
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 1339 104
Size: 1235 3147
PvuII CAG'CTG
Cuts at: 1807 2167 1807
Size: 360 4022
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SacII CC_GC'GG
Cuts at: 927 927
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 487 2405 2615 487
Size: 1918 210 2254
Fragments arranged by size:
2254 1918 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfoI GGC'GCC
Cuts at: 998 1814 2062 998
Size: 816 248 3318
Fragments arranged by size:
3318 816 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SgrAI Cr'CCGG_yG
Cuts at: 999 999
Size: 4382
SmaI CCC'GGG
Cuts at: 592 1404 592
Size: 812 3570
SmlI C'TyrA_G
Cuts at: 1135 1537 2452 2944 3206 1135
Size: 402 915 492 262 2311
Fragments arranged by size:
2311 915 492 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 873 1516 1870 107
Size: 766 643 354 2619
Fragments arranged by size:
2619 766 643 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1142 1965 2098 2557 2766 1142
Size: 823 133 459 209 2758
Fragments arranged by size:
2758 823 459 209 133
TatI w'GTAC_w
Cuts at: 746 1139 3897 746
Size: 393 2758 1231
Fragments arranged by size:
2758 1231 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 711 743 3899 3931 711
Size: 32 3156 32 1162
Fragments arranged by size:
3156 1162 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1199 1877 1199
Size: 678 3704
XmaI C'CCGG_G
Cuts at: 590 1402 590
Size: 812 3570
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BssHII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI