Restriction map for
pF1KE0549
(Circular) (Six-base) MAPSORT of: pF1KE0549.seq Check: 6794 from: 1 to: 3575
pF1KE0549 3575 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 613 613
Size: 3575
Acc65I G'GTAC_C
Cuts at: 591 591
Size: 3575
AclI AA'CG_TT
Cuts at: 3415 3415
Size: 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 506 548 1400 1832 2579 2847 243
Size: 263 42 852 432 747 268 971
Fragments arranged by size:
971 852 747 432 268 263 42
AflIII A'CryG_T
Cuts at: 927 2031 3008 927
Size: 1104 977 1494
Fragments arranged by size:
1494 1104 977
AgeI A'CCGG_T
Cuts at: 1963 1963
Size: 3575
AlwNI CAG_nnn'CTG
Cuts at: 880 2447 880
Size: 1567 2008
ApaLI G'TGCA_C
Cuts at: 2345 2345
Size: 3575
ApoI r'AATT_y
Cuts at: 511 579 2928 3184 511
Size: 68 2349 256 902
Fragments arranged by size:
2349 902 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 3575
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
AvaI C'yCGr_G
Cuts at: 595 595
Size: 3575
BamHI G'GATC_C
Cuts at: 600 600
Size: 3575
BanI G'GyrC_C
Cuts at: 591 1005 1253 1288 591
Size: 414 248 35 2878
Fragments arranged by size:
2878 414 248 35
BanII G_rGCy'C
Cuts at: 589 1619 589
Size: 1030 2545
BbeI G_GCGC'C
Cuts at: 1009 1257 1009
Size: 248 3327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 451 485 451
Size: 34 3541
BciVI GTATCCnnnnn_n'
Cuts at: 1473 2234 3575 1473
Size: 761 1341 1473
Fragments arranged by size:
1473 1341 761
BclI T'GATC_A
Cuts at: 1095 1095
Size: 3575
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3575
BglII A'GATC_T
Cuts at: 326 1090 3127 326
Size: 764 2037 774
Fragments arranged by size:
2037 774 764
BlpI GC'TnA_GC
Cuts at: 687 687
Size: 3575
Bme1580I G_kGCm'C
Cuts at: 1200 1293 2349 1200
Size: 93 1056 2426
Fragments arranged by size:
2426 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 787 868 1203 3347 787
Size: 81 335 2144 1015
Fragments arranged by size:
2144 1015 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1942 1942
Size: 3575
Bpu10I CC'TnA_GC
Cuts at: 458 458
Size: 3575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1630 2122 2420 751
Size: 879 492 298 1906
Fragments arranged by size:
1906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3279 3279
Size: 3575
BsaAI yAC'GTr
Cuts at: 928 928
Size: 3575
BsaBI GATnn'nnATC
Cuts at: 6 1089 3126 6
Size: 1083 2037 455
Fragments arranged by size:
2037 1083 455
BsaHI Gr'CG_yC
Cuts at: 1006 1254 1956 3452 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 702 248
BsaWI w'CCGG_w
Cuts at: 764 1285 1963 2237 2384 2795 764
Size: 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 452 1000 1758 2335 2831 452
Size: 548 758 577 496 1196
Fragments arranged by size:
1196 758 577 548 496
BsiEI CG_ry'CG
Cuts at: 104 1117 1163 2371 104
Size: 1013 46 1208 1308
Fragments arranged by size:
1308 1208 1013 46
BsiHKAI G_wGCw'C
Cuts at: 464 589 1367 1557 2349 464
Size: 125 778 190 792 1690
Fragments arranged by size:
1690 792 778 190 125
BsmI GAATG_Cn'
Cuts at: 528 528
Size: 3575
Bsp1286I G_dGCh'C
Cuts at: 464 589 1200 1293 1367 1557 1619 2349 464
Size: 125 611 93 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 190 125 93 74 62
BspEI T'CCGG_A
Cuts at: 2795 2795
Size: 3575
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 348 625 839 1141 1522 348
Size: 277 214 302 381 2401
Fragments arranged by size:
2401 381 302 277 214
BsrBI CCG'CTC
Cuts at: 50 1867 1921 3405 3570 50
Size: 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1487 1487
Size: 3575
BsrFI r'CCGG_y
Cuts at: 1573 1754 1963 1573
Size: 181 209 3185
Fragments arranged by size:
3185 209 181
BssSI C'ACGA_G
Cuts at: 1846 2204 1846
Size: 358 3217
BstAPI GCAn_nnn'nTGC
Cuts at: 952 952
Size: 3575
BstBI TT'CG_AA
Cuts at: 1936 1936
Size: 3575
BstYI r'GATC_y
Cuts at: 1 326 600 1067 1090 1425 1671 2683 2769
Size: 325 274 467 23 335 246 1012 86
Cuts at: 2769 2781 3127 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 325 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 1706 1869 1525
Size: 181 163 3231
Fragments arranged by size:
3231 181 163
BtsI GCAGTG_nn'
Cuts at: 818 818
Size: 3575
DraI TTT'AAA
Cuts at: 318 574 318
Size: 256 3319
DrdI GACnn_nn'nnGTC
Cuts at: 1281 2139 1281
Size: 858 2717
EaeI y'GGCC_r
Cuts at: 113 1160 1334 1725 1752 3097 113
Size: 1047 174 391 27 1345 591
Fragments arranged by size:
1345 1047 591 391 174 27
EagI C'GGCC_G
Cuts at: 1160 1160
Size: 3575
EarI CTCTTCn'nnn_
Cuts at: 524 1598 1808 524
Size: 1074 210 2291
Fragments arranged by size:
2291 1074 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1783 2093 2239 3385 1783
Size: 310 146 1146 1973
Fragments arranged by size:
1973 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 587
Size: 3575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 506 548 1400 1832 2579 2847 243
Size: 263 42 852 432 747 268 971
Fragments arranged by size:
971 852 747 432 268 263 42
EcoO109I rG'GnC_Cy
Cuts at: 714 714
Size: 3575
EcoRI G'AATT_C
Cuts at: 579 579
Size: 3575
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 1594 273
Size: 1321 2254
FspI TGC'GCA
Cuts at: 1356 1356
Size: 3575
HaeII r_GCGC'y
Cuts at: 1009 1257 2279 3258 1009
Size: 248 1022 979 1326
Fragments arranged by size:
1326 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1417 1447 1449 1479 1417
Size: 30 2 30 3513
Fragments arranged by size:
3513 30 30 2
HincII GTy'rAC
Cuts at: 614 614
Size: 3575
KasI G'GCGC_C
Cuts at: 1005 1253 1005
Size: 248 3327
KpnI G_GTAC'C
Cuts at: 595 595
Size: 3575
MluI A'CGCG_T
Cuts at: 3008 3008
Size: 3575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2246 2430 2246
Size: 184 3391
MscI TGG'CCA
Cuts at: 1336 1336
Size: 3575
MslI CAynn'nnrTG
Cuts at: 1564 1691 1564
Size: 127 3448
MspA1I CmG'CkG
Cuts at: 686 1000 1360 2373 2618 686
Size: 314 360 1013 245 1643
Fragments arranged by size:
1643 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1756 1756
Size: 3575
NarI GG'CG_CC
Cuts at: 1006 1254 1006
Size: 248 3327
NcoI C'CATG_G
Cuts at: 107 107
Size: 3575
NdeI CA'TA_TG
Cuts at: 243 243
Size: 3575
NgoMIV G'CCGG_C
Cuts at: 1754 1754
Size: 3575
NspI r_CATG'y
Cuts at: 628 1128 2035 628
Size: 500 907 2168
Fragments arranged by size:
2168 907 500
PciI A'CATG_T
Cuts at: 2031 2031
Size: 3575
PmeI GTTT'AAAC
Cuts at: 574 574
Size: 3575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 3543
PstI C_TGCA'G
Cuts at: 622 1307 622
Size: 685 2890
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3575
PvuII CAG'CTG
Cuts at: 1000 1360 1000
Size: 360 3215
RsrII CG'GwC_CG
Cuts at: 1770 1770
Size: 3575
SacI G_AGCT'C
Cuts at: 589 589
Size: 3575
SalI G'TCGA_C
Cuts at: 612 612
Size: 3575
SapI GCTCTTCn'nnn_
Cuts at: 1598 1808 1598
Size: 210 3365
SbfI CC_TGCA'GG
Cuts at: 622 622
Size: 3575
ScaI AGT'ACT
Cuts at: 3092 3092
Size: 3575
SfcI C'TryA_G
Cuts at: 33 618 1303 2296 2487 33
Size: 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 191
SfoI GGC'GCC
Cuts at: 1007 1255 1007
Size: 248 3327
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3575
SmaI CCC'GGG
Cuts at: 597 597
Size: 3575
SmlI C'TyrA_G
Cuts at: 730 1645 2137 2399 730
Size: 915 492 262 1906
Fragments arranged by size:
1906 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3575
SphI G_CATG'C
Cuts at: 628 1128 628
Size: 500 3075
StyI C'CwwG_G
Cuts at: 107 709 1063 107
Size: 602 354 2619
Fragments arranged by size:
2619 602 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 350 1158 1291 1750 1959 350
Size: 808 133 459 209 1966
Fragments arranged by size:
1966 808 459 209 133
TatI w'GTAC_w
Cuts at: 3090 3090
Size: 3575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3543
Tth111I GACn'n_nGTC
Cuts at: 1372 1372
Size: 3575
XbaI T'CTAG_A
Cuts at: 606 606
Size: 3575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1070 1070
Size: 3575
XmaI C'CCGG_G
Cuts at: 595 595
Size: 3575
XmnI GAAnn'nnTTC
Cuts at: 169 169
Size: 3575
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BpmI BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI