Restriction map for
pF1KE0550
(Circular) (Six-base) MAPSORT of: pF1KE0550.seq Check: 5875 from: 1 to: 4034
pF1KE0550 4034 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 733 1072 733
Size: 339 3695
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflIII A'CryG_T
Cuts at: 122 1386 2490 3467 122
Size: 1264 1104 977 689
Fragments arranged by size:
1264 1104 977 689
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 1339 2906 1339
Size: 1567 2467
ApaLI G'TGCA_C
Cuts at: 2804 2804
Size: 4034
ApoI r'AATT_y
Cuts at: 324 478 878 892 924 1038 3387 3643 324
Size: 154 400 14 32 114 2349 256 715
Fragments arranged by size:
2349 715 400 256 154 114 32 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 496 839 1048 2078 496
Size: 343 209 1030 2452
Fragments arranged by size:
2452 1030 343 209
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BbsI GAAGACnn'nnnn_
Cuts at: 141 141
Size: 4034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 960 994 960
Size: 34 4000
BciVI GTATCCnnnnn_n'
Cuts at: 1932 2693 4034 1932
Size: 761 1341 1932
Fragments arranged by size:
1932 1341 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 602 1549 3586 602
Size: 947 2037 1050
Fragments arranged by size:
2037 1050 947
BlpI GC'TnA_GC
Cuts at: 1146 1146
Size: 4034
Bme1580I G_kGCm'C
Cuts at: 1659 1752 2808 1659
Size: 93 1056 2885
Fragments arranged by size:
2885 1056 93
BmgBI CAC'GTC
Cuts at: 931 931
Size: 4034
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BmtI G_CTAG'C
Cuts at: 202 202
Size: 4034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 788 881 986 1010 376
Size: 412 93 105 24 3400
Fragments arranged by size:
3400 412 105 93 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2089 2581 2879 1210
Size: 879 492 298 2365
Fragments arranged by size:
2365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 619 3738 619
Size: 3119 915
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 583 1548 3585 6
Size: 577 965 2037 455
Fragments arranged by size:
2037 965 577 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 148
Size: 30 4004
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 742 742
Size: 4034
BseYI C'CCAG_C
Cuts at: 774 838 1459 2217 2794 3290 774
Size: 64 621 758 577 496 1518
Fragments arranged by size:
1518 758 621 577 496 64
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1048 1826 2016 2808 1048
Size: 778 190 792 2274
Fragments arranged by size:
2274 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 496 839 1048 1659 1752 1826 2016 2078 2808
Size: 343 209 611 93 74 190 62 730
Cuts at: 2808 496
Size: 1722
Fragments arranged by size:
1722 730 611 343 209 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 3210 3210
Size: 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1084 1298 1600 1981 1084
Size: 214 302 381 3137
Fragments arranged by size:
3137 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1003 1946 1003
Size: 943 3091
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstYI r'GATC_y
Cuts at: 1 602 796 1059 1526 1549 1884 2130 3142
Size: 601 194 263 467 23 335 246 1012
Cuts at: 3142 3228 3240 3586 1
Size: 86 12 346 449
Fragments arranged by size:
1012 601 467 449 346 335 263 246
194 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 650 1277 650
Size: 627 3407
DraI TTT'AAA
Cuts at: 897 1033 897
Size: 136 3898
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 464 1619 1793 2184 2211 3556 464
Size: 1155 174 391 27 1345 942
Fragments arranged by size:
1345 1155 942 391 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 947 2057 2267 947
Size: 1110 210 2714
Fragments arranged by size:
2714 1110 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2242 2552 2698 3844 2242
Size: 310 146 1146 2432
Fragments arranged by size:
2432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1046
Size: 4034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 788 881 986 1010 1859 2291 3038 3306
Size: 412 93 105 24 849 432 747 268
Cuts at: 3306 376
Size: 1104
Fragments arranged by size:
1104 849 747 432 412 268 105 93
24
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 4034
EcoO109I rG'GnC_Cy
Cuts at: 205 1173 205
Size: 968 3066
EcoRI G'AATT_C
Cuts at: 324 878 1038 324
Size: 554 160 3320
Fragments arranged by size:
3320 554 160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1876 1906 1908 1938 1876
Size: 30 2 30 3972
Fragments arranged by size:
3972 30 30 2
HincII GTy'rAC
Cuts at: 453 734 1073 453
Size: 281 339 3414
Fragments arranged by size:
3414 339 281
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 507 2705 2889 507
Size: 2198 184 1652
Fragments arranged by size:
2198 1652 184
MscI TGG'CCA
Cuts at: 466 1795 466
Size: 1329 2705
MslI CAynn'nnrTG
Cuts at: 670 2023 2150 670
Size: 1353 127 2554
Fragments arranged by size:
2554 1353 127
MspA1I CmG'CkG
Cuts at: 179 1145 1459 1819 2832 3077 179
Size: 966 314 360 1013 245 1136
Fragments arranged by size:
1136 1013 966 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NheI G'CTAG_C
Cuts at: 198 198
Size: 4034
NspI r_CATG'y
Cuts at: 126 1087 1587 2494 126
Size: 961 500 907 1666
Fragments arranged by size:
1666 961 907 500
PasI CC'CwG_GG
Cuts at: 143 690 143
Size: 547 3487
PciI A'CATG_T
Cuts at: 122 2490 122
Size: 2368 1666
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 330 2229 330
Size: 1899 2135
SacI G_AGCT'C
Cuts at: 1048 1048
Size: 4034
SalI G'TCGA_C
Cuts at: 732 1071 732
Size: 339 3695
SapI GCTCTTCn'nnn_
Cuts at: 2057 2267 2057
Size: 210 3824
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 3551 3551
Size: 4034
SfcI C'TryA_G
Cuts at: 33 1077 1762 2755 2946 33
Size: 1044 685 993 191 1121
Fragments arranged by size:
1121 1044 993 685 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 1189 2104 2596 2858 1189
Size: 915 492 262 2365
Fragments arranged by size:
2365 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StyI C'CwwG_G
Cuts at: 1168 1522 1168
Size: 354 3680
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1617 1750 2209 2418 1617
Size: 133 459 209 3233
Fragments arranged by size:
3233 459 209 133
TatI w'GTAC_w
Cuts at: 3549 3549
Size: 4034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI