Restriction map for
pF1KE0551
(Circular) (Six-base) MAPSORT of: pF1KE0551.seq Check: 6699 from: 1 to: 3974
pF1KE0551 3974 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 290 308 1012 290
Size: 18 704 3252
Fragments arranged by size:
3252 704 18
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 370 3814 370
Size: 3444 530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1799 2231 2978 3246 1799
Size: 432 747 268 2527
Fragments arranged by size:
2527 747 432 268
AflIII A'CryG_T
Cuts at: 701 905 1326 2430 3407 701
Size: 204 421 1104 977 1268
Fragments arranged by size:
1268 1104 977 421 204
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AleI CACnn'nnGTG
Cuts at: 910 910
Size: 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 460 460
Size: 3974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 580
Size: 32 3942
AlwNI CAG_nnn'CTG
Cuts at: 492 648 1279 2846 492
Size: 156 631 1567 1620
Fragments arranged by size:
1620 1567 631 156
ApaI G_GGCC'C
Cuts at: 126 126
Size: 3974
ApaLI G'TGCA_C
Cuts at: 2744 2744
Size: 3974
ApoI r'AATT_y
Cuts at: 978 3327 3583 978
Size: 2349 256 1369
Fragments arranged by size:
2349 1369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 994 994
Size: 3974
BamHI G'GATC_C
Cuts at: 540 999 540
Size: 459 3515
BanI G'GyrC_C
Cuts at: 277 517 790 990 1404 1652 1687 277
Size: 240 273 200 414 248 35 2564
Fragments arranged by size:
2564 414 273 248 240 200 35
BanII G_rGCy'C
Cuts at: 126 367 757 988 2018 126
Size: 241 390 231 1030 2082
Fragments arranged by size:
2082 1030 390 241 231
BbeI G_GCGC'C
Cuts at: 794 1408 1656 794
Size: 614 248 3112
Fragments arranged by size:
3112 614 248
BbsI GAAGACnn'nnnn_
Cuts at: 890 890
Size: 3974
BbvCI CC'TCA_GC
Cuts at: 802 941 802
Size: 139 3835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 557 591 90
Size: 34 433 34 3473
Fragments arranged by size:
3473 433 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BglI GCCn_nnn'nGGC
Cuts at: 130 714 130
Size: 584 3390
BglII A'GATC_T
Cuts at: 1489 3526 1489
Size: 2037 1937
BlpI GC'TnA_GC
Cuts at: 1086 1086
Size: 3974
Bme1580I G_kGCm'C
Cuts at: 126 1599 1692 2748 126
Size: 1473 93 1056 1352
Fragments arranged by size:
1473 1352 1056 93
BmgBI CAC'GTC
Cuts at: 908 908
Size: 3974
BmrI ACTGGGnnnn_n'
Cuts at: 370 1186 1267 1602 3746 370
Size: 816 81 335 2144 598
Fragments arranged by size:
2144 816 598 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 3974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 946
Size: 3974
Bpu10I CC'TnA_GC
Cuts at: 802 941 802
Size: 139 3835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2029 2521 2819 1150
Size: 879 492 298 2305
Fragments arranged by size:
2305 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 704 1327 704
Size: 623 3351
BsaBI GATnn'nnATC
Cuts at: 6 736 1488 3525 6
Size: 730 752 2037 455
Fragments arranged by size:
2037 752 730 455
BsaHI Gr'CG_yC
Cuts at: 791 1405 1653 2355 3851 791
Size: 614 248 702 1496 914
Fragments arranged by size:
1496 914 702 614 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 384 414 580 610 384
Size: 30 166 30 3748
Fragments arranged by size:
3748 166 30 30
BseYI C'CCAG_C
Cuts at: 448 882 1399 2157 2734 3230 448
Size: 434 517 758 577 496 1192
Fragments arranged by size:
1192 758 577 517 496 434
BsiEI CG_ry'CG
Cuts at: 104 698 1516 1562 2770 104
Size: 594 818 46 1208 1308
Fragments arranged by size:
1308 1208 818 594 46
BsiHKAI G_wGCw'C
Cuts at: 988 1766 1956 2748 988
Size: 778 190 792 2214
Fragments arranged by size:
2214 792 778 190
BsmI GAATG_Cn'
Cuts at: 333 501 333
Size: 168 3806
Bsp1286I G_dGCh'C
Cuts at: 126 367 757 988 1599 1692 1766 1956 2018
Size: 241 390 231 611 93 74 190 62
Cuts at: 2018 2748 126
Size: 730 1352
Fragments arranged by size:
1352 730 611 390 241 231 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 452 538 1024 1238 1540 1921 176
Size: 276 86 486 214 302 381 2229
Fragments arranged by size:
2229 486 381 302 276 214 86
BsrBI CCG'CTC
Cuts at: 50 117 722 2266 2320 3804 3969 50
Size: 67 605 1544 54 1484 165 55
Fragments arranged by size:
1544 1484 605 165 67 55 54
BsrDI GCAATG_nn'
Cuts at: 794 1886 794
Size: 1092 2882
BsrFI r'CCGG_y
Cuts at: 713 1972 2153 2362 713
Size: 1259 181 209 2325
Fragments arranged by size:
2325 1259 209 181
BssSI C'ACGA_G
Cuts at: 283 808 2245 2603 283
Size: 525 1437 358 1654
Fragments arranged by size:
1654 1437 525 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1351 1351
Size: 3974
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstXI CCAn_nnnn'nTGG
Cuts at: 630 882 630
Size: 252 3722
BstYI r'GATC_y
Cuts at: 1 488 540 999 1466 1489 1824 2070 3082
Size: 487 52 459 467 23 335 246 1012
Cuts at: 3082 3168 3180 3526 1
Size: 86 12 346 449
Fragments arranged by size:
1012 487 467 459 449 346 335 246
86 52 23 12
BtgI C'CryG_G
Cuts at: 584 623 584
Size: 39 3935
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1924 2105 2268 1924
Size: 181 163 3630
Fragments arranged by size:
3630 181 163
BtsI GCAGTG_nn'
Cuts at: 444 1217 444
Size: 773 3201
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DrdI GACnn_nn'nnGTC
Cuts at: 651 1680 2538 651
Size: 1029 858 2087
Fragments arranged by size:
2087 1029 858
EaeI y'GGCC_r
Cuts at: 182 706 1559 1733 2124 2151 3496 182
Size: 524 853 174 391 27 1345 660
Fragments arranged by size:
1345 853 660 524 391 174 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 732 2182 2492 2638 3784 732
Size: 1450 310 146 1146 922
Fragments arranged by size:
1450 1146 922 310 146
EcoICRI GAG'CTC
Cuts at: 986 986
Size: 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1799 2231 2978 3246 946
Size: 853 432 747 268 1674
Fragments arranged by size:
1674 853 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 123 267 400 527 555 887 1113 123
Size: 144 133 127 28 332 226 2984
Fragments arranged by size:
2984 332 226 144 133 127 28
EcoRI G'AATT_C
Cuts at: 978 978
Size: 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 794 1408 1656 2678 3657 794
Size: 614 248 1022 979 1111
Fragments arranged by size:
1111 1022 979 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 716 748 1816 1846 1848 1878 384
Size: 32 300 32 1068 30 2 30 2480
Fragments arranged by size:
2480 1068 300 32 32 30 30 2
HincII GTy'rAC
Cuts at: 309 1013 309
Size: 704 3270
KasI G'GCGC_C
Cuts at: 790 1404 1652 790
Size: 614 248 3112
Fragments arranged by size:
3112 614 248
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 2645 2829 635
Size: 2010 184 1780
Fragments arranged by size:
2010 1780 184
MscI TGG'CCA
Cuts at: 184 708 1735 184
Size: 524 1027 2423
Fragments arranged by size:
2423 1027 524
MslI CAynn'nnrTG
Cuts at: 910 1963 2090 910
Size: 1053 127 2794
Fragments arranged by size:
2794 1053 127
MspA1I CmG'CkG
Cuts at: 1085 1399 1759 2772 3017 1085
Size: 314 360 1013 245 2042
Fragments arranged by size:
2042 1013 360 314 245
NaeI GCC'GGC
Cuts at: 715 2155 715
Size: 1440 2534
NarI GG'CG_CC
Cuts at: 791 1405 1653 791
Size: 614 248 3112
Fragments arranged by size:
3112 614 248
NgoMIV G'CCGG_C
Cuts at: 713 2153 713
Size: 1440 2534
NspI r_CATG'y
Cuts at: 252 1027 1527 2434 252
Size: 775 500 907 1792
Fragments arranged by size:
1792 907 775 500
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PfoI T'CCnGG_A
Cuts at: 322 962 322
Size: 640 3334
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PmlI CAC'GTG
Cuts at: 704 704
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 580 612 3113 3145 580
Size: 32 2501 32 1409
Fragments arranged by size:
2501 1409 32 32
PpuMI rG'GwC_Cy
Cuts at: 400 527 400
Size: 127 3847
PspOMI G'GGCC_C
Cuts at: 122 122
Size: 3974
PstI C_TGCA'G
Cuts at: 173 439 1021 1706 173
Size: 266 582 685 2441
Fragments arranged by size:
2441 685 582 266
PvuI CG_AT'CG
Cuts at: 104 698 104
Size: 594 3380
PvuII CAG'CTG
Cuts at: 1399 1759 1399
Size: 360 3614
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 988 988
Size: 3974
SalI G'TCGA_C
Cuts at: 307 1011 307
Size: 704 3270
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SexAI A'CCwGG_T
Cuts at: 503 503
Size: 3974
SfcI C'TryA_G
Cuts at: 33 169 435 1017 1702 2695 2886 33
Size: 136 266 582 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 266 191 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 130 130
Size: 3974
SfoI GGC'GCC
Cuts at: 792 1406 1654 792
Size: 614 248 3112
Fragments arranged by size:
3112 614 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 996 996
Size: 3974
SmlI C'TyrA_G
Cuts at: 1129 2044 2536 2798 1129
Size: 915 492 262 2305
Fragments arranged by size:
2305 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 1027 1527 1027
Size: 500 3474
StuI AGG'CCT
Cuts at: 481 481
Size: 3974
StyI C'CwwG_G
Cuts at: 230 476 1108 1462 230
Size: 246 632 354 2742
Fragments arranged by size:
2742 632 354 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 505 1557 1690 2149 2358 295
Size: 210 1052 133 459 209 1911
Fragments arranged by size:
1911 1052 459 210 209 133
TatI w'GTAC_w
Cuts at: 590 3489 590
Size: 2899 1075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 3942
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 357 1469 228
Size: 129 1112 2733
Fragments arranged by size:
2733 1112 129
XmaI C'CCGG_G
Cuts at: 994 994
Size: 3974
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BfrBI BmtI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PshAI PsiI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI