Restriction map for
pF1KE0552
(Circular) (Six-base) MAPSORT of: pF1KE0552.seq Check: 2586 from: 1 to: 4019
pF1KE0552 4019 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 752 1057 752
Size: 305 3714
Acc65I G'GTAC_C
Cuts at: 1035 1035
Size: 4019
AclI AA'CG_TT
Cuts at: 3859 3859
Size: 4019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 601 1844 2276 3023 3291 583
Size: 18 1243 432 747 268 1311
Fragments arranged by size:
1311 1243 747 432 268 18
AflIII A'CryG_T
Cuts at: 873 1371 2475 3452 873
Size: 498 1104 977 1440
Fragments arranged by size:
1440 1104 977 498
AgeI A'CCGG_T
Cuts at: 2407 2407
Size: 4019
AleI CACnn'nnGTG
Cuts at: 931 931
Size: 4019
AlwNI CAG_nnn'CTG
Cuts at: 594 1324 2891 594
Size: 730 1567 1722
Fragments arranged by size:
1722 1567 730
ApaLI G'TGCA_C
Cuts at: 2789 2789
Size: 4019
ApoI r'AATT_y
Cuts at: 1023 3372 3628 1023
Size: 2349 256 1414
Fragments arranged by size:
2349 1414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
AvaI C'yCGr_G
Cuts at: 333 408 1039 333
Size: 75 631 3313
Fragments arranged by size:
3313 631 75
BamHI G'GATC_C
Cuts at: 1044 1044
Size: 4019
BanI G'GyrC_C
Cuts at: 146 270 1035 1449 1697 1732 146
Size: 124 765 414 248 35 2433
Fragments arranged by size:
2433 765 414 248 124 35
BanII G_rGCy'C
Cuts at: 407 1033 2063 407
Size: 626 1030 2363
Fragments arranged by size:
2363 1030 626
BbeI G_GCGC'C
Cuts at: 1453 1701 1453
Size: 248 3771
BciVI GTATCCnnnnn_n'
Cuts at: 1917 2678 4019 1917
Size: 761 1341 1917
Fragments arranged by size:
1917 1341 761
BclI T'GATC_A
Cuts at: 1539 1539
Size: 4019
BglII A'GATC_T
Cuts at: 1534 3571 1534
Size: 2037 1982
BlpI GC'TnA_GC
Cuts at: 1131 1131
Size: 4019
Bme1580I G_kGCm'C
Cuts at: 1644 1737 2793 1644
Size: 93 1056 2870
Fragments arranged by size:
2870 1056 93
BmgBI CAC'GTC
Cuts at: 369 369
Size: 4019
BmrI ACTGGGnnnn_n'
Cuts at: 114 790 804 1231 1312 1647 3791 114
Size: 676 14 427 81 335 2144 342
Fragments arranged by size:
2144 676 427 342 335 81 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 2386 204
Size: 2182 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 742 278
Size: 464 3555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1195 2074 2566 2864 1195
Size: 879 492 298 2350
Fragments arranged by size:
2350 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3723 3723
Size: 4019
BsaAI yAC'GTr
Cuts at: 129 1372 129
Size: 1243 2776
BsaBI GATnn'nnATC
Cuts at: 6 469 1533 3570 6
Size: 463 1064 2037 455
Fragments arranged by size:
2037 1064 463 455
BsaHI Gr'CG_yC
Cuts at: 1450 1698 2400 3896 1450
Size: 248 702 1496 1573
Fragments arranged by size:
1573 1496 702 248
BsaWI w'CCGG_w
Cuts at: 647 1208 1729 2407 2681 2828 3239 647
Size: 561 521 678 274 147 411 1427
Fragments arranged by size:
1427 678 561 521 411 274 147
BseYI C'CCAG_C
Cuts at: 382 399 438 686 1444 2202 2779 3275 382
Size: 17 39 248 758 758 577 496 1126
Fragments arranged by size:
1126 758 758 577 496 248 39 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 607 868 274
Size: 333 261 3425
Fragments arranged by size:
3425 333 261
BsiEI CG_ry'CG
Cuts at: 104 1561 1607 2815 104
Size: 1457 46 1208 1308
Fragments arranged by size:
1457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1033 1811 2001 2793 1033
Size: 778 190 792 2259
Fragments arranged by size:
2259 792 778 190
BsiWI C'GTAC_G
Cuts at: 359 359
Size: 4019
BsmI GAATG_Cn'
Cuts at: 112 942 112
Size: 830 3189
BsmBI CGTCTCn'nnnn_
Cuts at: 324 324
Size: 4019
Bsp1286I G_dGCh'C
Cuts at: 407 1033 1644 1737 1811 2001 2063 2793 407
Size: 626 611 93 74 190 62 730 1633
Fragments arranged by size:
1633 730 626 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 647 3239 647
Size: 2592 1427
BspHI T'CATG_A
Cuts at: 3195 3195
Size: 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1069 1283 1585 1966 1069
Size: 214 302 381 3122
Fragments arranged by size:
3122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 139 2311 2365 3849 4014 50
Size: 89 2172 54 1484 165 55
Fragments arranged by size:
2172 1484 165 89 55 54
BsrDI GCAATG_nn'
Cuts at: 1931 1931
Size: 4019
BsrFI r'CCGG_y
Cuts at: 158 2017 2198 2407 158
Size: 1859 181 209 1770
Fragments arranged by size:
1859 1770 209 181
BssSI C'ACGA_G
Cuts at: 484 2290 2648 484
Size: 1806 358 1855
Fragments arranged by size:
1855 1806 358
BstAPI GCAn_nnn'nTGC
Cuts at: 594 1396 594
Size: 802 3217
BstBI TT'CG_AA
Cuts at: 2380 2380
Size: 4019
BstEII G'GTnAC_C
Cuts at: 630 630
Size: 4019
BstYI r'GATC_y
Cuts at: 1 650 1044 1511 1534 1869 2115 3127 3213
Size: 649 394 467 23 335 246 1012 86
Cuts at: 3213 3225 3571 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 394 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 664 768 865 107
Size: 557 104 97 3261
Fragments arranged by size:
3261 557 104 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1969 2150 2313 1969
Size: 181 163 3675
Fragments arranged by size:
3675 181 163
BtsI GCAGTG_nn'
Cuts at: 1262 1262
Size: 4019
DraI TTT'AAA
Cuts at: 1018 1018
Size: 4019
DrdI GACnn_nn'nnGTC
Cuts at: 628 1725 2583 628
Size: 1097 858 2064
Fragments arranged by size:
2064 1097 858
EaeI y'GGCC_r
Cuts at: 134 512 882 1604 1778 2169 2196 3541 134
Size: 378 370 722 174 391 27 1345 612
Fragments arranged by size:
1345 722 612 391 378 370 174 27
EagI C'GGCC_G
Cuts at: 1604 1604
Size: 4019
EarI CTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2227 2537 2683 3829 2227
Size: 310 146 1146 2417
Fragments arranged by size:
2417 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1031 1031
Size: 4019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 583 601 742 1844 2276 3023 3291 278
Size: 305 18 141 1102 432 747 268 1006
Fragments arranged by size:
1102 1006 747 432 305 268 141 18
EcoO109I rG'GnC_Cy
Cuts at: 798 1158 798
Size: 360 3659
EcoRI G'AATT_C
Cuts at: 1023 1023
Size: 4019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 549 2038 549
Size: 1489 2530
FspI TGC'GCA
Cuts at: 1800 1800
Size: 4019
HaeII r_GCGC'y
Cuts at: 1453 1701 2723 3702 1453
Size: 248 1022 979 1770
Fragments arranged by size:
1770 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 316 348 1861 1891 1893 1923 123
Size: 32 161 32 1513 30 2 30 2219
Fragments arranged by size:
2219 1513 161 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1058 1058
Size: 4019
KasI G'GCGC_C
Cuts at: 1449 1697 1449
Size: 248 3771
KpnI G_GTAC'C
Cuts at: 1039 1039
Size: 4019
MluI A'CGCG_T
Cuts at: 3452 3452
Size: 4019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 215 2690 2874 215
Size: 2475 184 1360
Fragments arranged by size:
2475 1360 184
MscI TGG'CCA
Cuts at: 884 1780 884
Size: 896 3123
MslI CAynn'nnrTG
Cuts at: 172 352 931 2008 2135 172
Size: 180 579 1077 127 2056
Fragments arranged by size:
2056 1077 579 180 127
MspA1I CmG'CkG
Cuts at: 816 867 1130 1444 1804 2817 3062 816
Size: 51 263 314 360 1013 245 1773
Fragments arranged by size:
1773 1013 360 314 263 245 51
NaeI GCC'GGC
Cuts at: 160 2200 160
Size: 2040 1979
NarI GG'CG_CC
Cuts at: 1450 1698 1450
Size: 248 3771
NcoI C'CATG_G
Cuts at: 107 664 768 107
Size: 557 104 3358
Fragments arranged by size:
3358 557 104
NgoMIV G'CCGG_C
Cuts at: 158 2198 158
Size: 2040 1979
NspI r_CATG'y
Cuts at: 253 877 1072 1572 2479 253
Size: 624 195 500 907 1793
Fragments arranged by size:
1793 907 624 500 195
PciI A'CATG_T
Cuts at: 873 2475 873
Size: 1602 2417
PflMI CCAn_nnn'nTGG
Cuts at: 132 132
Size: 4019
PfoI T'CCnGG_A
Cuts at: 788 788
Size: 4019
PmeI GTTT'AAAC
Cuts at: 1018 1018
Size: 4019
PmlI CAC'GTG
Cuts at: 129 129
Size: 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3987
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 4019
PspXI vC'TCGA_Gb
Cuts at: 333 333
Size: 4019
PstI C_TGCA'G
Cuts at: 1066 1751 1066
Size: 685 3334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4019
PvuII CAG'CTG
Cuts at: 1444 1804 1444
Size: 360 3659
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 4019
SacI G_AGCT'C
Cuts at: 1033 1033
Size: 4019
SacII CC_GC'GG
Cuts at: 868 868
Size: 4019
SalI G'TCGA_C
Cuts at: 1056 1056
Size: 4019
SanDI GG'GwC_CC
Cuts at: 798 798
Size: 4019
SapI GCTCTTCn'nnn_
Cuts at: 2042 2252 2042
Size: 210 3809
SbfI CC_TGCA'GG
Cuts at: 1066 1066
Size: 4019
ScaI AGT'ACT
Cuts at: 3536 3536
Size: 4019
SfcI C'TryA_G
Cuts at: 33 1062 1747 2740 2931 33
Size: 1029 685 993 191 1121
Fragments arranged by size:
1121 1029 993 685 191
SfoI GGC'GCC
Cuts at: 1451 1699 1451
Size: 248 3771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4019
SmaI CCC'GGG
Cuts at: 1041 1041
Size: 4019
SmlI C'TyrA_G
Cuts at: 333 1174 2089 2581 2843 333
Size: 841 915 492 262 1509
Fragments arranged by size:
1509 915 841 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4019
SphI G_CATG'C
Cuts at: 1072 1572 1072
Size: 500 3519
StuI AGG'CCT
Cuts at: 205 205
Size: 4019
StyI C'CwwG_G
Cuts at: 107 664 768 1153 1507 107
Size: 557 104 385 354 2619
Fragments arranged by size:
2619 557 385 354 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 1602 1735 2194 2403 214
Size: 1388 133 459 209 1830
Fragments arranged by size:
1830 1388 459 209 133
TatI w'GTAC_w
Cuts at: 182 458 3534 182
Size: 276 3076 667
Fragments arranged by size:
3076 667 276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 770 802 3536 3568 770
Size: 32 2734 32 1221
Fragments arranged by size:
2734 1221 32 32
Tth111I GACn'n_nGTC
Cuts at: 796 1816 796
Size: 1020 2999
XbaI T'CTAG_A
Cuts at: 1050 1050
Size: 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 671 898 1514 671
Size: 227 616 3176
Fragments arranged by size:
3176 616 227
XhoI C'TCGA_G
Cuts at: 333 333
Size: 4019
XmaI C'CCGG_G
Cuts at: 1039 1039
Size: 4019
XmnI GAAnn'nnTTC
Cuts at: 424 424
Size: 4019
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspOMI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI