Restriction map for
pF1KE0553
(Circular) (Six-base) MAPSORT of: pF1KE0553.seq Check: 695 from: 1 to: 4184
pF1KE0553 4184 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 1178 4024 1178
Size: 2846 1338
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 2009 2441 3188 3456 907
Size: 1102 432 747 268 1635
Fragments arranged by size:
1635 1102 747 432 268
AflIII A'CryG_T
Cuts at: 936 1536 2640 3617 936
Size: 600 1104 977 1503
Fragments arranged by size:
1503 1104 977 600
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AleI CACnn'nnGTG
Cuts at: 937 1075 937
Size: 138 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 330 361 330
Size: 31 4153
AlwNI CAG_nnn'CTG
Cuts at: 1489 3056 1489
Size: 1567 2617
ApaI G_GGCC'C
Cuts at: 1026 1026
Size: 4184
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 424 562 625 1188 3537 3793 424
Size: 138 63 563 2349 256 815
Fragments arranged by size:
2349 815 563 256 138 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1204 1204
Size: 4184
BamHI G'GATC_C
Cuts at: 687 1209 687
Size: 522 3662
BanI G'GyrC_C
Cuts at: 740 784 1200 1614 1862 1897 740
Size: 44 416 414 248 35 3027
Fragments arranged by size:
3027 416 414 248 44 35
BanII G_rGCy'C
Cuts at: 537 880 1026 1198 2228 537
Size: 343 146 172 1030 2493
Fragments arranged by size:
2493 1030 343 172 146
BbeI G_GCGC'C
Cuts at: 744 1618 1866 744
Size: 874 248 3062
Fragments arranged by size:
3062 874 248
BciVI GTATCCnnnnn_n'
Cuts at: 393 2082 2843 4184 393
Size: 1689 761 1341 393
Fragments arranged by size:
1689 1341 761 393
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BfrBI ATG'CAT
Cuts at: 643 643
Size: 4184
BglII A'GATC_T
Cuts at: 845 1699 3736 845
Size: 854 2037 1293
Fragments arranged by size:
2037 1293 854
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 1026 1809 1902 2958 1026
Size: 783 93 1056 2252
Fragments arranged by size:
2252 1056 783 93
BmrI ACTGGGnnnn_n'
Cuts at: 495 1396 1477 1812 3956 495
Size: 901 81 335 2144 723
Fragments arranged by size:
2144 901 723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1147 894
Size: 253 3931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2239 2731 3029 1360
Size: 879 492 298 2515
Fragments arranged by size:
2515 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 939 1537 939
Size: 598 3586
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 741 1615 1863 2565 4061 741
Size: 874 248 702 1496 864
Fragments arranged by size:
1496 874 864 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 524 554 127
Size: 30 367 30 3757
Fragments arranged by size:
3757 367 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1135 1135
Size: 4184
BseYI C'CCAG_C
Cuts at: 575 1018 1064 1609 2367 2944 3440 575
Size: 443 46 545 758 577 496 1319
Fragments arranged by size:
1319 758 577 545 496 443 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 1127 1198 1976 2166 2958 440
Size: 687 71 778 190 792 1666
Fragments arranged by size:
1666 792 778 687 190 71
BsmI GAATG_Cn'
Cuts at: 643 643
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 440 537 880 1026 1127 1198 1809 1902 1976
Size: 97 343 146 101 71 611 93 74
Cuts at: 1976 2166 2228 2958 440
Size: 190 62 730 1666
Fragments arranged by size:
1666 730 611 343 190 146 101 97
93 74 71 62
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 1037 3360 1037
Size: 2323 1861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1234 1448 1750 2131 1234
Size: 214 302 381 3287
Fragments arranged by size:
3287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 242 2476 2530 4014 4179 50
Size: 88 104 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 104 88 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BsrGI T'GTAC_A
Cuts at: 590 590
Size: 4184
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 612 1008 1561 612
Size: 396 553 3235
Fragments arranged by size:
3235 553 396
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstYI r'GATC_y
Cuts at: 1 687 845 1209 1676 1699 2034 2280 3292
Size: 686 158 364 467 23 335 246 1012
Cuts at: 3292 3378 3390 3736 1
Size: 86 12 346 449
Fragments arranged by size:
1012 686 467 449 364 346 335 246
158 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 265 727 1890 2748 265
Size: 462 1163 858 1701
Fragments arranged by size:
1701 1163 858 462
EaeI y'GGCC_r
Cuts at: 419 1769 1943 2334 2361 3706 419
Size: 1350 174 391 27 1345 897
Fragments arranged by size:
1350 1345 897 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 453 2392 2702 2848 3994 453
Size: 1939 310 146 1146 643
Fragments arranged by size:
1939 1146 643 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 907 1147 2009 2441 3188 3456 894
Size: 13 240 862 432 747 268 1622
Fragments arranged by size:
1622 862 747 432 268 240 13
EcoNI CCTnn'n_nnAGG
Cuts at: 988 988
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 792 1022 1323 792
Size: 230 301 3653
Fragments arranged by size:
3653 301 230
EcoRI G'AATT_C
Cuts at: 424 1188 424
Size: 764 3420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 949 1965 949
Size: 1016 3168
HaeII r_GCGC'y
Cuts at: 511 744 774 1000 1618 1866 2888 3867 511
Size: 233 30 226 618 248 1022 979 828
Fragments arranged by size:
1022 979 828 618 248 233 226 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 935 967 2026 2056 2058 2088 111
Size: 32 792 32 1059 30 2 30 2207
Fragments arranged by size:
2207 1059 792 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
KasI G'GCGC_C
Cuts at: 740 1614 1862 740
Size: 874 248 3062
Fragments arranged by size:
3062 874 248
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 175 812 2855 3039 175
Size: 637 2043 184 1320
Fragments arranged by size:
2043 1320 637 184
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 443 937 1075 2173 2300 443
Size: 494 138 1098 127 2327
Fragments arranged by size:
2327 1098 494 138 127
MspA1I CmG'CkG
Cuts at: 345 1295 1609 1969 2982 3227 345
Size: 950 314 360 1013 245 1302
Fragments arranged by size:
1302 1013 950 360 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 741 1615 1863 741
Size: 874 248 3062
Fragments arranged by size:
3062 874 248
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NsiI A_TGCA'T
Cuts at: 645 645
Size: 4184
NspI r_CATG'y
Cuts at: 1237 1737 2644 1237
Size: 500 907 2777
Fragments arranged by size:
2777 907 500
PasI CC'CwG_GG
Cuts at: 1106 1106
Size: 4184
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 810 810
Size: 4184
PfoI T'CCnGG_A
Cuts at: 385 871 385
Size: 486 3698
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PmlI CAC'GTG
Cuts at: 939 939
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 202 3323 3355 170
Size: 32 3121 32 999
Fragments arranged by size:
3121 999 32 32
PpuMI rG'GwC_Cy
Cuts at: 792 792
Size: 4184
PspOMI G'GGCC_C
Cuts at: 1022 1022
Size: 4184
PstI C_TGCA'G
Cuts at: 517 1231 1916 517
Size: 714 685 2785
Fragments arranged by size:
2785 714 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 345 1609 1969 345
Size: 1264 360 2560
Fragments arranged by size:
2560 1264 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 1060 3701 1060
Size: 2641 1543
SexAI A'CCwGG_T
Cuts at: 373 373
Size: 4184
SfcI C'TryA_G
Cuts at: 33 513 849 1227 1912 2905 3096 33
Size: 480 336 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 480 378 336 191
SfoI GGC'GCC
Cuts at: 742 1616 1864 742
Size: 874 248 3062
Fragments arranged by size:
3062 874 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 1339 2254 2746 3008 1339
Size: 915 492 262 2515
Fragments arranged by size:
2515 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 616 1318 1672 616
Size: 702 354 3128
Fragments arranged by size:
3128 702 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 590 1058 3699 590
Size: 468 2641 1075
Fragments arranged by size:
2641 1075 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 3701 3733 524
Size: 32 3145 32 975
Fragments arranged by size:
3145 975 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1108 1138 1679 1108
Size: 30 541 3613
Fragments arranged by size:
3613 541 30
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI PacI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI