Restriction map for
pF1KE0556
(Circular) (Six-base) MAPSORT of: pF1KE0556.seq Check: 8174 from: 1 to: 3866
pF1KE0556 3866 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 904 904
Size: 3866
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1691 2123 2870 3138 253
Size: 1438 432 747 268 981
Fragments arranged by size:
1438 981 747 432 268
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AhdI GACnn_n'nnGTC
Cuts at: 262 262
Size: 3866
AlwNI CAG_nnn'CTG
Cuts at: 147 161 277 1171 2738 147
Size: 14 116 894 1567 1275
Fragments arranged by size:
1567 1275 894 116 14
ApaI G_GGCC'C
Cuts at: 300 697 762 300
Size: 397 65 3404
Fragments arranged by size:
3404 397 65
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 709 870 3219 3475 709
Size: 161 2349 256 1100
Fragments arranged by size:
2349 1100 256 161
AseI AT'TA_AT
Cuts at: 20 20
Size: 3866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 886 886
Size: 3866
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 882 1296 1544 1579 882
Size: 414 248 35 3169
Fragments arranged by size:
3169 414 248 35
BanII G_rGCy'C
Cuts at: 221 300 697 762 880 1910 221
Size: 79 397 65 118 1030 2177
Fragments arranged by size:
2177 1030 397 118 79 65
BbeI G_GCGC'C
Cuts at: 1300 1548 1300
Size: 248 3618
BbvCI CC'TCA_GC
Cuts at: 324 605 676 324
Size: 281 71 3514
Fragments arranged by size:
3514 281 71
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 1386 1386
Size: 3866
BglI GCCn_nnn'nGGC
Cuts at: 225 328 225
Size: 103 3763
BglII A'GATC_T
Cuts at: 1381 3418 1381
Size: 2037 1829
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 141 300 697 762 1491 1584 2640 141
Size: 159 397 65 729 93 1056 1367
Fragments arranged by size:
1367 1056 729 397 159 93 65
BmrI ACTGGGnnnn_n'
Cuts at: 1078 1159 1494 3638 1078
Size: 81 335 2144 1306
Fragments arranged by size:
2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 3866
Bpu10I CC'TnA_GC
Cuts at: 324 605 676 324
Size: 281 71 3514
Fragments arranged by size:
3514 281 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1921 2413 2711 1042
Size: 879 492 298 2197
Fragments arranged by size:
2197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 3570 475
Size: 3095 771
BsaAI yAC'GTr
Cuts at: 774 1219 774
Size: 445 3421
BsaBI GATnn'nnATC
Cuts at: 6 1380 3417 6
Size: 1374 2037 455
Fragments arranged by size:
2037 1374 455
BsaHI Gr'CG_yC
Cuts at: 1297 1545 2247 3743 1297
Size: 248 702 1496 1420
Fragments arranged by size:
1496 1420 702 248
BsaWI w'CCGG_w
Cuts at: 1055 1576 2254 2528 2675 3086 1055
Size: 521 678 274 147 411 1835
Fragments arranged by size:
1835 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 583 693 379
Size: 204 110 3552
Fragments arranged by size:
3552 204 110
BseYI C'CCAG_C
Cuts at: 165 399 612 690 855 1291 2049 2626 3122
Size: 234 213 78 165 436 758 577 496
Cuts at: 3122 165
Size: 909
Fragments arranged by size:
909 758 577 496 436 234 213 165
78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 676 796 174
Size: 502 120 3244
Fragments arranged by size:
3244 502 120
BsiEI CG_ry'CG
Cuts at: 104 1408 1454 2662 104
Size: 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 46
BsiHKAI G_wGCw'C
Cuts at: 880 1658 1848 2640 880
Size: 778 190 792 2106
Fragments arranged by size:
2106 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 141 221 300 697 762 880 1491 1584 1658
Size: 80 79 397 65 118 611 93 74
Cuts at: 1658 1848 1910 2640 141
Size: 190 62 730 1367
Fragments arranged by size:
1367 730 611 397 190 118 93 80
79 74 65 62
BspEI T'CCGG_A
Cuts at: 3086 3086
Size: 3866
BspHI T'CATG_A
Cuts at: 3042 3042
Size: 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 916 1130 1432 1813 916
Size: 214 302 381 2969
Fragments arranged by size:
2969 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2158 2212 3696 3861 50
Size: 2108 54 1484 165 55
Fragments arranged by size:
2108 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 311 1778 311
Size: 1467 2399
BsrFI r'CCGG_y
Cuts at: 151 1864 2045 2254 151
Size: 1713 181 209 1763
Fragments arranged by size:
1763 1713 209 181
BsrGI T'GTAC_A
Cuts at: 422 422
Size: 3866
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 816 1243 816
Size: 427 3439
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstEII G'GTnAC_C
Cuts at: 309 309
Size: 3866
BstYI r'GATC_y
Cuts at: 1 824 891 1358 1381 1716 1962 2974 3060
Size: 823 67 467 23 335 246 1012 86
Cuts at: 3060 3072 3418 1
Size: 12 346 449
Fragments arranged by size:
1012 823 467 449 346 335 246 86
67 23 12
BtgI C'CryG_G
Cuts at: 107 335 384 431 107
Size: 228 49 47 3542
Fragments arranged by size:
3542 228 49 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 1109
Size: 3866
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 477 1451 1625 2016 2043 3388 477
Size: 974 174 391 27 1345 955
Fragments arranged by size:
1345 974 955 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 385 1889 2099 385
Size: 1504 210 2152
Fragments arranged by size:
2152 1504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 878 878
Size: 3866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1691 2123 2870 3138 253
Size: 1438 432 747 268 981
Fragments arranged by size:
1438 981 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 499 499
Size: 3866
EcoO109I rG'GnC_Cy
Cuts at: 170 297 389 408 694 759 1005 170
Size: 127 92 19 286 65 246 3031
Fragments arranged by size:
3031 286 246 127 92 65 19
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3866
FspI TGC'GCA
Cuts at: 211 1647 211
Size: 1436 2430
HaeII r_GCGC'y
Cuts at: 686 853 1300 1548 2570 3549 686
Size: 167 447 248 1022 979 1003
Fragments arranged by size:
1022 1003 979 447 248 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 1708 1738 1740 1770 248
Size: 32 1428 30 2 30 2344
Fragments arranged by size:
2344 1428 32 30 30 2
HincII GTy'rAC
Cuts at: 905 905
Size: 3866
KasI G'GCGC_C
Cuts at: 1296 1544 1296
Size: 248 3618
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 2537 2721 533
Size: 2004 184 1678
Fragments arranged by size:
2004 1678 184
MscI TGG'CCA
Cuts at: 1627 1627
Size: 3866
MslI CAynn'nnrTG
Cuts at: 271 1855 1982 271
Size: 1584 127 2155
Fragments arranged by size:
2155 1584 127
MspA1I CmG'CkG
Cuts at: 328 399 585 604 660 675 780 847 977
Size: 71 186 19 56 15 105 67 130
Cuts at: 977 1291 1651 2664 2909 328
Size: 314 360 1013 245 1285
Fragments arranged by size:
1285 1013 360 314 245 186 130 105
71 67 56 19 15
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 1297 1545 1297
Size: 248 3618
NcoI C'CATG_G
Cuts at: 107 335 384 107
Size: 228 49 3589
Fragments arranged by size:
3589 228 49
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PasI CC'CwG_GG
Cuts at: 133 133
Size: 3866
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PfoI T'CCnGG_A
Cuts at: 827 827
Size: 3866
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PmlI CAC'GTG
Cuts at: 774 774
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3834
PpuMI rG'GwC_Cy
Cuts at: 170 389 170
Size: 219 3647
PspOMI G'GGCC_C
Cuts at: 296 693 758 296
Size: 397 65 3404
Fragments arranged by size:
3404 397 65
PstI C_TGCA'G
Cuts at: 398 584 590 913 1598 398
Size: 186 6 323 685 2666
Fragments arranged by size:
2666 685 323 186 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 399 585 604 675 1291 1651 399
Size: 186 19 71 616 360 2614
Fragments arranged by size:
2614 616 360 186 71 19
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 880 880
Size: 3866
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SanDI GG'GwC_CC
Cuts at: 389 389
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 385 1889 2099 385
Size: 1504 210 2152
Fragments arranged by size:
2152 1504 210
SbfI CC_TGCA'GG
Cuts at: 913 913
Size: 3866
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 394 580 586 909 1594 2587 2778 33
Size: 361 186 6 323 685 993 191 1121
Fragments arranged by size:
1121 993 685 361 323 191 186 6
SfoI GGC'GCC
Cuts at: 1298 1546 1298
Size: 248 3618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 888 888
Size: 3866
SmlI C'TyrA_G
Cuts at: 1021 1936 2428 2690 1021
Size: 915 492 262 2197
Fragments arranged by size:
2197 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3866
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
StyI C'CwwG_G
Cuts at: 107 335 384 1000 1354 107
Size: 228 49 616 354 2619
Fragments arranged by size:
2619 616 354 228 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1449 1582 2041 2250 1449
Size: 133 459 209 3065
Fragments arranged by size:
3065 459 209 133
TatI w'GTAC_w
Cuts at: 185 422 3381 185
Size: 237 2959 670
Fragments arranged by size:
2959 670 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 3834
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 897 897
Size: 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 1361 462
Size: 899 2967
XmaI C'CCGG_G
Cuts at: 886 886
Size: 3866
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BpmI
BsaXI BsiWI BsmI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI