Restriction map for
pF1KE0559
(Circular) (Six-base) MAPSORT of: pF1KE0559.seq Check: 9683 from: 1 to: 3998
pF1KE0559 3998 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 440 440
Size: 3998
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 623 1014 623
Size: 391 3607
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1823 2255 3002 3270 1823
Size: 432 747 268 2551
Fragments arranged by size:
2551 747 432 268
AfeI AGC'GCT
Cuts at: 931 931
Size: 3998
AflIII A'CryG_T
Cuts at: 785 1350 2454 3431 785
Size: 565 1104 977 1352
Fragments arranged by size:
1352 1104 977 565
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AleI CACnn'nnGTG
Cuts at: 265 265
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 279 459 1303 2870 279
Size: 180 844 1567 1407
Fragments arranged by size:
1567 1407 844 180
ApaI G_GGCC'C
Cuts at: 197 197
Size: 3998
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 256 342 623 1014 1428 1676 1711 256
Size: 86 281 391 414 248 35 2543
Fragments arranged by size:
2543 414 391 281 248 86 35
BanII G_rGCy'C
Cuts at: 197 327 848 1012 2042 197
Size: 130 521 164 1030 2153
Fragments arranged by size:
2153 1030 521 164 130
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 695 907 695
Size: 212 3786
BbvCI CC'TCA_GC
Cuts at: 973 973
Size: 3998
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglI GCCn_nnn'nGGC
Cuts at: 414 414
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 652 1110 652
Size: 458 3540
Bme1580I G_kGCm'C
Cuts at: 197 347 1623 1716 2772 197
Size: 150 1276 93 1056 1423
Fragments arranged by size:
1423 1276 1056 150 93
BmgBI CAC'GTC
Cuts at: 510 788 510
Size: 278 3720
BmrI ACTGGGnnnn_n'
Cuts at: 199 1210 1291 1626 3770 199
Size: 1011 81 335 2144 427
Fragments arranged by size:
2144 1011 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 406 620 751 163
Size: 243 214 131 3410
Fragments arranged by size:
3410 243 214 131
Bpu10I CC'TnA_GC
Cuts at: 557 973 557
Size: 416 3582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1174 2053 2545 2843 373
Size: 801 879 492 298 1528
Fragments arranged by size:
1528 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 754 3702 522
Size: 232 2948 818
Fragments arranged by size:
2948 818 232
BsaAI yAC'GTr
Cuts at: 150 336 633 1351 150
Size: 186 297 718 2797
Fragments arranged by size:
2797 718 297 186
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 847
Size: 30 3968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 772
Size: 3998
BseYI C'CCAG_C
Cuts at: 889 1423 2181 2758 3254 889
Size: 534 758 577 496 1633
Fragments arranged by size:
1633 758 577 534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 3998
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 848 1012 1790 1980 2772 848
Size: 164 778 190 792 2074
Fragments arranged by size:
2074 792 778 190 164
BsiWI C'GTAC_G
Cuts at: 336 336
Size: 3998
Bsp1286I G_dGCh'C
Cuts at: 197 327 347 848 1012 1623 1716 1790 1980
Size: 130 20 501 164 611 93 74 190
Cuts at: 1980 2042 2772 197
Size: 62 730 1423
Fragments arranged by size:
1423 730 611 501 190 164 130 93
74 62 20
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 872 3174 872
Size: 2302 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 440 1048 1262 1564 1945 236
Size: 204 608 214 302 381 2289
Fragments arranged by size:
2289 608 381 302 214 204
BsrBI CCG'CTC
Cuts at: 50 165 983 2290 2344 3828 3993 50
Size: 115 818 1307 54 1484 165 55
Fragments arranged by size:
1484 1307 818 165 115 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 725 1023 1490 1513 1848 2094 3106 3192
Size: 724 298 467 23 335 246 1012 86
Cuts at: 3192 3204 3550 1
Size: 12 346 449
Fragments arranged by size:
1012 724 467 449 346 335 298 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 378 378
Size: 3998
BtgI C'CryG_G
Cuts at: 107 412 764 914 107
Size: 305 352 150 3191
Fragments arranged by size:
3191 352 305 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1241
Size: 3998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 491 526 491
Size: 35 3963
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 514 1704 2562 514
Size: 1190 858 1950
Fragments arranged by size:
1950 1190 858
EaeI y'GGCC_r
Cuts at: 1583 1757 2148 2175 3520 1583
Size: 174 391 27 1345 2061
Fragments arranged by size:
2061 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 685 2021 2231 685
Size: 1336 210 2452
Fragments arranged by size:
2452 1336 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 846 1010 846
Size: 164 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 406 620 751 1823 2255 3002 3270 163
Size: 243 214 131 1072 432 747 268 891
Fragments arranged by size:
1072 891 747 432 268 243 214 131
EcoNI CCTnn'n_nnAGG
Cuts at: 754 873 754
Size: 119 3879
EcoO109I rG'GnC_Cy
Cuts at: 159 193 194 566 617 1137 159
Size: 34 1 372 51 520 3020
Fragments arranged by size:
3020 520 372 51 34 1
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 924 2017 924
Size: 1093 2905
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 747 933 1432 1680 2702 3681 747
Size: 186 499 248 1022 979 1064
Fragments arranged by size:
1064 1022 979 499 248 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 627 1018 627
Size: 391 3607
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 2669 2853 443
Size: 2226 184 1588
Fragments arranged by size:
2226 1588 184
MscI TGG'CCA
Cuts at: 1759 1759
Size: 3998
MslI CAynn'nnrTG
Cuts at: 265 1987 2114 265
Size: 1722 127 2149
Fragments arranged by size:
2149 1722 127
MspA1I CmG'CkG
Cuts at: 1109 1423 1783 2796 3041 1109
Size: 314 360 1013 245 2066
Fragments arranged by size:
2066 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 107 764 107
Size: 657 3341
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PflMI CCAn_nnn'nTGG
Cuts at: 279 540 279
Size: 261 3737
PfoI T'CCnGG_A
Cuts at: 119 581 728 119
Size: 462 147 3389
Fragments arranged by size:
3389 462 147
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PmlI CAC'GTG
Cuts at: 633 633
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PpuMI rG'GwC_Cy
Cuts at: 159 566 617 159
Size: 407 51 3540
Fragments arranged by size:
3540 407 51
PspOMI G'GGCC_C
Cuts at: 193 193
Size: 3998
PstI C_TGCA'G
Cuts at: 203 970 1045 1730 203
Size: 767 75 685 2471
Fragments arranged by size:
2471 767 685 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 1423 1783 1423
Size: 360 3638
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 848 1012 848
Size: 164 3834
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SanDI GG'GwC_CC
Cuts at: 159 159
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 175 3515 175
Size: 3340 658
SfcI C'TryA_G
Cuts at: 33 199 966 1041 1726 2719 2910 33
Size: 166 767 75 685 993 191 1121
Fragments arranged by size:
1121 993 767 685 191 166 75
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 352 1153 2068 2560 2822 352
Size: 801 915 492 262 1528
Fragments arranged by size:
1528 915 801 492 262
SnaBI TAC'GTA
Cuts at: 336 336
Size: 3998
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StyI C'CwwG_G
Cuts at: 107 764 1132 1486 107
Size: 657 368 354 2619
Fragments arranged by size:
2619 657 368 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 255 676 1581 1714 2173 2382 255
Size: 421 905 133 459 209 1871
Fragments arranged by size:
1871 905 459 421 209 133
TatI w'GTAC_w
Cuts at: 173 3513 173
Size: 3340 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 3515 3547 847
Size: 32 2636 32 1298
Fragments arranged by size:
2636 1298 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 771 912 1493 771
Size: 141 581 3276
Fragments arranged by size:
3276 581 141
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI