Restriction map for
pF1KE0560
(Circular) (Six-base) MAPSORT of: pF1KE0560.seq Check: 255 from: 1 to: 4505
pF1KE0560 4505 bp
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AccI GT'mk_AC
Cuts at: 866 1543 866
Size: 677 3828
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2762 3509 3777 2330
Size: 432 747 268 3058
Fragments arranged by size:
3058 747 432 268
AflII C'TTAA_G
Cuts at: 147 547 147
Size: 400 4105
AflIII A'CryG_T
Cuts at: 1857 2961 3938 1857
Size: 1104 977 2424
Fragments arranged by size:
2424 1104 977
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 931 963 931
Size: 32 4473
AlwNI CAG_nnn'CTG
Cuts at: 741 1053 1074 1218 1810 3377 741
Size: 312 21 144 592 1567 1869
Fragments arranged by size:
1869 1567 592 312 144 21
ApaI G_GGCC'C
Cuts at: 1170 1170
Size: 4505
ApaLI G'TGCA_C
Cuts at: 3275 3275
Size: 4505
ApoI r'AATT_y
Cuts at: 238 1302 1347 1509 3858 4114 238
Size: 1064 45 162 2349 256 629
Fragments arranged by size:
2349 1064 629 256 162 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 858 1525 858
Size: 667 3838
BamHI G'GATC_C
Cuts at: 130 1530 130
Size: 1400 3105
BanI G'GyrC_C
Cuts at: 298 1521 1935 2183 2218 298
Size: 1223 414 248 35 2585
Fragments arranged by size:
2585 1223 414 248 35
BanII G_rGCy'C
Cuts at: 426 656 1170 1519 2549 426
Size: 230 514 349 1030 2382
Fragments arranged by size:
2382 1030 514 349 230
BbeI G_GCGC'C
Cuts at: 1939 2187 1939
Size: 248 4257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 895 929 895
Size: 34 4471
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 507 2025 507
Size: 1518 2987
BglI GCCn_nnn'nGGC
Cuts at: 616 616
Size: 4505
BglII A'GATC_T
Cuts at: 980 2020 4057 980
Size: 1040 2037 1428
Fragments arranged by size:
2037 1428 1040
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 303 1170 2130 2223 3279 303
Size: 867 960 93 1056 1529
Fragments arranged by size:
1529 1056 960 867 93
BmrI ACTGGGnnnn_n'
Cuts at: 1717 1798 2133 4277 1717
Size: 81 335 2144 1945
Fragments arranged by size:
2144 1945 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 448 1075 1117 1192 1240 176
Size: 272 627 42 75 48 3441
Fragments arranged by size:
3441 627 272 75 48 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3052 3350 1681
Size: 879 492 298 2836
Fragments arranged by size:
2836 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1108 1195 1264 4209 1108
Size: 87 69 2945 1404
Fragments arranged by size:
2945 1404 87 69
BsaAI yAC'GTr
Cuts at: 1858 1858
Size: 4505
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 1936 2184 2886 4382 1936
Size: 248 702 1496 2059
Fragments arranged by size:
2059 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3167 3314 3725 1694
Size: 521 678 274 147 411 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 931 961 931
Size: 30 4475
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 445 555 778 811 1189 1225 340
Size: 105 110 223 33 378 36 3620
Fragments arranged by size:
3620 378 223 110 105 36 33
BseYI C'CCAG_C
Cuts at: 823 1930 2688 3265 3761 823
Size: 1107 758 577 496 1567
Fragments arranged by size:
1567 1107 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 1168 209
Size: 959 3546
BsiEI CG_ry'CG
Cuts at: 104 2047 2093 3301 104
Size: 1943 46 1208 1308
Fragments arranged by size:
1943 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 426 656 1105 1519 2297 2487 3279 426
Size: 230 449 414 778 190 792 1652
Fragments arranged by size:
1652 792 778 449 414 230 190
Bsp1286I G_dGCh'C
Cuts at: 303 426 656 1105 1170 1519 2130 2223 2297
Size: 123 230 449 65 349 611 93 74
Cuts at: 2297 2487 2549 3279 303
Size: 190 62 730 1529
Fragments arranged by size:
1529 730 611 449 349 230 190 123
93 74 65 62
BspEI T'CCGG_A
Cuts at: 3725 3725
Size: 4505
BspHI T'CATG_A
Cuts at: 510 3681 510
Size: 3171 1334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1555 1769 2071 2452 1555
Size: 214 302 381 3608
Fragments arranged by size:
3608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 898 2797 2851 4335 4500 50
Size: 848 1899 54 1484 165 55
Fragments arranged by size:
1899 1484 848 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 2417
Size: 4505
BsrFI r'CCGG_y
Cuts at: 2503 2684 2893 2503
Size: 181 209 4115
Fragments arranged by size:
4115 209 181
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 196 1882 196
Size: 1686 2819
BstBI TT'CG_AA
Cuts at: 2866 2866
Size: 4505
BstEII G'GTnAC_C
Cuts at: 369 369
Size: 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 295 295
Size: 4505
BstYI r'GATC_y
Cuts at: 1 130 163 490 543 980 1058 1148 1530
Size: 129 33 327 53 437 78 90 382
Cuts at: 1530 1997 2020 2355 2601 3613 3699 3711 4057
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4057 1
Size: 449
Fragments arranged by size:
1012 467 449 437 382 346 335 327
246 129 90 86 78 53 33 23
12
BtgI C'CryG_G
Cuts at: 498 498
Size: 4505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 213 276 820 1748 213
Size: 63 544 928 2970
Fragments arranged by size:
2970 928 544 63
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3069 2211
Size: 858 3647
EaeI y'GGCC_r
Cuts at: 1079 2090 2264 2655 2682 4027 1079
Size: 1011 174 391 27 1345 1557
Fragments arranged by size:
1557 1345 1011 391 174 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4505
EarI CTCTTCn'nnn_
Cuts at: 788 952 1391 2528 2738 788
Size: 164 439 1137 210 2555
Fragments arranged by size:
2555 1137 439 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3023 3169 4315 2713
Size: 310 146 1146 2903
Fragments arranged by size:
2903 1146 310 146
EcoICRI GAG'CTC
Cuts at: 424 654 1517 424
Size: 230 863 3412
Fragments arranged by size:
3412 863 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 448 1075 1117 1192 1240 2330 2762 3509
Size: 272 627 42 75 48 1090 432 747
Cuts at: 3509 3777 176
Size: 268 904
Fragments arranged by size:
1090 904 747 627 432 272 268 75
48 42
EcoO109I rG'GnC_Cy
Cuts at: 1166 1167 1644 1166
Size: 1 477 4027
Fragments arranged by size:
4027 477 1
EcoRI G'AATT_C
Cuts at: 1347 1509 1347
Size: 162 4343
EcoRV GAT'ATC
Cuts at: 526 526
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1269 1511 2524 1269
Size: 242 1013 3250
Fragments arranged by size:
3250 1013 242
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 965 1939 2187 3209 4188 965
Size: 974 248 1022 979 1282
Fragments arranged by size:
1282 1022 979 974 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 759 791 792 824 1140 1172 2347 2377 2379
Size: 32 1 32 316 32 1175 30 2
Cuts at: 2379 2409 759
Size: 30 2855
Fragments arranged by size:
2855 1175 316 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1544 1544
Size: 4505
KasI G'GCGC_C
Cuts at: 1935 2183 1935
Size: 248 4257
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MfeI C'AATT_G
Cuts at: 536 536
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3176 3360 3176
Size: 184 4321
MscI TGG'CCA
Cuts at: 1081 2266 1081
Size: 1185 3320
MslI CAynn'nnrTG
Cuts at: 293 1030 2494 2621 293
Size: 737 1464 127 2177
Fragments arranged by size:
2177 1464 737 127
MspA1I CmG'CkG
Cuts at: 1065 1140 1182 1616 1930 2290 3303 3548 1065
Size: 75 42 434 314 360 1013 245 2022
Fragments arranged by size:
2022 1013 434 360 314 245 75 42
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4505
NarI GG'CG_CC
Cuts at: 1936 2184 1936
Size: 248 4257
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4505
NspI r_CATG'y
Cuts at: 142 215 894 1558 2058 2965 142
Size: 73 679 664 500 907 1682
Fragments arranged by size:
1682 907 679 664 500 73
PasI CC'CwG_GG
Cuts at: 1342 1342
Size: 4505
PciI A'CATG_T
Cuts at: 2961 2961
Size: 4505
PflMI CCAn_nnn'nTGG
Cuts at: 1218 1218
Size: 4505
PfoI T'CCnGG_A
Cuts at: 704 704
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 931 963 3644 3676 931
Size: 32 2681 32 1760
Fragments arranged by size:
2681 1760 32 32
PspOMI G'GGCC_C
Cuts at: 1166 1166
Size: 4505
PstI C_TGCA'G
Cuts at: 599 727 815 974 1049 1070 1552 2237 599
Size: 128 88 159 75 21 482 685 2867
Fragments arranged by size:
2867 685 482 159 128 88 75 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 1065 1140 1182 1930 2290 1065
Size: 75 42 748 360 3280
Fragments arranged by size:
3280 748 360 75 42
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 426 656 1519 426
Size: 230 863 3412
Fragments arranged by size:
3412 863 230
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 952 1391 2528 2738 952
Size: 439 1137 210 2719
Fragments arranged by size:
2719 1137 439 210
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SexAI A'CCwGG_T
Cuts at: 1187 1187
Size: 4505
SfcI C'TryA_G
Cuts at: 33 516 595 723 811 970 1045 1066 1548
Size: 483 79 128 88 159 75 21 482
Cuts at: 1548 2233 3226 3417 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 482 191 159 128
88 79 75 21
SfoI GGC'GCC
Cuts at: 1937 2185 1937
Size: 248 4257
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 860 1527 860
Size: 667 3838
SmlI C'TyrA_G
Cuts at: 147 547 1660 2575 3067 3329 147
Size: 400 1113 915 492 262 1323
Fragments arranged by size:
1323 1113 915 492 400 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 1558 2058 1558
Size: 500 4005
StuI AGG'CCT
Cuts at: 943 943
Size: 4505
StyI C'CwwG_G
Cuts at: 251 303 1639 1993 251
Size: 52 1336 354 2763
Fragments arranged by size:
2763 1336 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 745 1132 1219 2088 2221 2680 2889 745
Size: 387 87 869 133 459 209 2361
Fragments arranged by size:
2361 869 459 387 209 133 87
TatI w'GTAC_w
Cuts at: 4020 4020
Size: 4505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4473
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 124 1374 2000 124
Size: 1250 626 2629
Fragments arranged by size:
2629 1250 626
XmaI C'CCGG_G
Cuts at: 858 1525 858
Size: 667 3838
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI