Restriction map for
pF1KE0563
(Circular) (Six-base) MAPSORT of: pF1KE0563.seq Check: 8224 from: 1 to: 3779
pF1KE0563 3779 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 817 817
Size: 3779
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 3619 3619
Size: 3779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1604 2036 2783 3051 670
Size: 934 432 747 268 1398
Fragments arranged by size:
1398 934 747 432 268
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 2167 2167
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 1084 2651 1084
Size: 1567 2212
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 205 783 3132 3388 205
Size: 578 2349 256 596
Fragments arranged by size:
2349 596 578 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 621 799 621
Size: 178 3601
BamHI G'GATC_C
Cuts at: 804 804
Size: 3779
BanI G'GyrC_C
Cuts at: 795 1209 1457 1492 795
Size: 414 248 35 3082
Fragments arranged by size:
3082 414 248 35
BanII G_rGCy'C
Cuts at: 378 793 1823 378
Size: 415 1030 2334
Fragments arranged by size:
2334 1030 415
BbeI G_GCGC'C
Cuts at: 1213 1461 1213
Size: 248 3531
BciVI GTATCCnnnnn_n'
Cuts at: 1677 2438 3779 1677
Size: 761 1341 1677
Fragments arranged by size:
1677 1341 761
BclI T'GATC_A
Cuts at: 1299 1299
Size: 3779
BglII A'GATC_T
Cuts at: 1294 3331 1294
Size: 2037 1742
BlpI GC'TnA_GC
Cuts at: 565 891 565
Size: 326 3453
Bme1580I G_kGCm'C
Cuts at: 1404 1497 2553 1404
Size: 93 1056 2630
Fragments arranged by size:
2630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 991 1072 1407 3551 991
Size: 81 335 2144 1219
Fragments arranged by size:
2144 1219 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 636 740 2146 636
Size: 104 1406 2269
Fragments arranged by size:
2269 1406 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 618 206
Size: 412 3367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 606 1132 606
Size: 526 3253
BsaBI GATnn'nnATC
Cuts at: 6 1293 3330 6
Size: 1287 2037 455
Fragments arranged by size:
2037 1287 455
BsaHI Gr'CG_yC
Cuts at: 1210 1458 2160 3656 1210
Size: 248 702 1496 1333
Fragments arranged by size:
1496 1333 702 248
BsaWI w'CCGG_w
Cuts at: 968 1489 2167 2441 2588 2999 968
Size: 521 678 274 147 411 1748
Fragments arranged by size:
1748 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 569 1204 1962 2539 3035 569
Size: 635 758 577 496 1313
Fragments arranged by size:
1313 758 635 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 325
Size: 3779
BsiEI CG_ry'CG
Cuts at: 104 1321 1367 2575 104
Size: 1217 46 1208 1308
Fragments arranged by size:
1308 1217 1208 46
BsiHKAI G_wGCw'C
Cuts at: 793 1571 1761 2553 793
Size: 778 190 792 2019
Fragments arranged by size:
2019 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 378 793 1404 1497 1571 1761 1823 2553 378
Size: 415 611 93 74 190 62 730 1604
Fragments arranged by size:
1604 730 611 415 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 686 829 1043 1345 1726 686
Size: 143 214 302 381 2739
Fragments arranged by size:
2739 381 302 214 143
BsrBI CCG'CTC
Cuts at: 50 2071 2125 3609 3774 50
Size: 2021 54 1484 165 55
Fragments arranged by size:
2021 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 329 1691 329
Size: 1362 2417
BsrFI r'CCGG_y
Cuts at: 1777 1958 2167 1777
Size: 181 209 3389
Fragments arranged by size:
3389 209 181
BsrGI T'GTAC_A
Cuts at: 389 389
Size: 3779
BssSI C'ACGA_G
Cuts at: 2050 2408 2050
Size: 358 3421
BstAPI GCAn_nnn'nTGC
Cuts at: 1156 1156
Size: 3779
BstBI TT'CG_AA
Cuts at: 2140 2140
Size: 3779
BstYI r'GATC_y
Cuts at: 1 804 1271 1294 1629 1875 2887 2973 2985
Size: 803 467 23 335 246 1012 86 12
Cuts at: 2985 3331 1
Size: 346 449
Fragments arranged by size:
1012 803 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 683 728 107
Size: 576 45 3158
Fragments arranged by size:
3158 576 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1729 1910 2073 1729
Size: 181 163 3435
Fragments arranged by size:
3435 181 163
BtsI GCAGTG_nn'
Cuts at: 1022 1022
Size: 3779
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DraIII CAC_nnn'GTG
Cuts at: 588 588
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 1364 1538 1929 1956 3301 1364
Size: 174 391 27 1345 1842
Fragments arranged by size:
1842 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1364 1364
Size: 3779
EarI CTCTTCn'nnn_
Cuts at: 194 605 1802 2012 194
Size: 411 1197 210 1961
Fragments arranged by size:
1961 1197 411 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1987 2297 2443 3589 1987
Size: 310 146 1146 2177
Fragments arranged by size:
2177 1146 310 146
EcoICRI GAG'CTC
Cuts at: 791 791
Size: 3779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 618 670 1604 2036 2783 3051 206
Size: 412 52 934 432 747 268 934
Fragments arranged by size:
934 934 747 432 412 268 52
EcoO109I rG'GnC_Cy
Cuts at: 654 918 654
Size: 264 3515
EcoRI G'AATT_C
Cuts at: 783 783
Size: 3779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 489 565 1798 489
Size: 76 1233 2470
Fragments arranged by size:
2470 1233 76
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 1213 1461 2483 3462 1213
Size: 248 1022 979 1530
Fragments arranged by size:
1530 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 724 756 1621 1651 1653 1683 724
Size: 32 865 30 2 30 2820
Fragments arranged by size:
2820 865 32 30 30 2
HincII GTy'rAC
Cuts at: 445 818 445
Size: 373 3406
KasI G'GCGC_C
Cuts at: 1209 1457 1209
Size: 248 3531
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 176 2450 2634 176
Size: 2274 184 1321
Fragments arranged by size:
2274 1321 184
MscI TGG'CCA
Cuts at: 1540 1540
Size: 3779
MslI CAynn'nnrTG
Cuts at: 1768 1895 1768
Size: 127 3652
MspA1I CmG'CkG
Cuts at: 184 890 1204 1564 2577 2822 184
Size: 706 314 360 1013 245 1141
Fragments arranged by size:
1141 1013 706 360 314 245
NaeI GCC'GGC
Cuts at: 1960 1960
Size: 3779
NarI GG'CG_CC
Cuts at: 1210 1458 1210
Size: 248 3531
NcoI C'CATG_G
Cuts at: 107 728 107
Size: 621 3158
NdeI CA'TA_TG
Cuts at: 439 439
Size: 3779
NgoMIV G'CCGG_C
Cuts at: 1958 1958
Size: 3779
NspI r_CATG'y
Cuts at: 832 1332 2239 832
Size: 500 907 2372
Fragments arranged by size:
2372 907 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PflMI CCAn_nnn'nTGG
Cuts at: 167 167
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PshAI GACnn'nnGTC
Cuts at: 623 623
Size: 3779
PspXI vC'TCGA_Gb
Cuts at: 621 621
Size: 3779
PstI C_TGCA'G
Cuts at: 183 222 704 826 1511 183
Size: 39 482 122 685 2451
Fragments arranged by size:
2451 685 482 122 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 184 1204 1564 184
Size: 1020 360 2399
Fragments arranged by size:
2399 1020 360
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 793 793
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 605 1802 2012 605
Size: 1197 210 2372
Fragments arranged by size:
2372 1197 210
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SexAI A'CCwGG_T
Cuts at: 584 584
Size: 3779
SfcI C'TryA_G
Cuts at: 33 179 218 700 822 1507 2500 2691 33
Size: 146 39 482 122 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 191 146 122 39
SfoI GGC'GCC
Cuts at: 1211 1459 1211
Size: 248 3531
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 801 801
Size: 3779
SmlI C'TyrA_G
Cuts at: 621 934 1849 2341 2603 621
Size: 313 915 492 262 1797
Fragments arranged by size:
1797 915 492 313 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
StyI C'CwwG_G
Cuts at: 107 473 728 913 1267 107
Size: 366 255 185 354 2619
Fragments arranged by size:
2619 366 354 255 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1362 1495 1954 2163 1362
Size: 133 459 209 2978
Fragments arranged by size:
2978 459 209 133
TatI w'GTAC_w
Cuts at: 389 3294 389
Size: 2905 874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 210 810 210
Size: 600 3179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1274 471
Size: 803 2976
XhoI C'TCGA_G
Cuts at: 621 621
Size: 3779
XmaI C'CCGG_G
Cuts at: 799 799
Size: 3779
XmnI GAAnn'nnTTC
Cuts at: 763 763
Size: 3779
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI