Restriction map for pF1KE0564
 (Circular) (Six-base) MAPSORT of: pF1KE0564.seq  Check: 9739  from: 1  to: 4457



pF1KE0564  4457 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AatII G_ACGT'C

Cuts at:   1262    1262 
   Size:       4457
 
AccI GT'mk_AC

Cuts at:   1495    1495 
   Size:       4457
 
Acc65I G'GTAC_C

Cuts at:   1473    1473 
   Size:       4457
 
AclI AA'CG_TT

Cuts at:   1187    4297    1187 
   Size:       3110    1347
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    633     796    1006    2282    2714    3461    3729     633 
   Size:        163     210    1276     432     747     268    1361

  Fragments arranged by size: 

                1361    1276     747     432     268     210     163
 
AflIII A'CryG_T

Cuts at:   1289    1809    2913    3890    1289 
   Size:        520    1104     977    1856

  Fragments arranged by size: 

                1856    1104     977     520
 
AgeI A'CCGG_T

Cuts at:   2845    2845 
   Size:       4457
 
AhdI GACnn_n'nnGTC

Cuts at:    537    1060     537 
   Size:        523    3934
 
AleI CACnn'nnGTG

Cuts at:   1366    1366 
   Size:       4457
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    485     485 
   Size:       4457
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    949     981     949 
   Size:         32    4425
 
AlwNI CAG_nnn'CTG

Cuts at:    317    1251    1762    3329     317 
   Size:        934     511    1567    1445

  Fragments arranged by size: 

                1567    1445     934     511
 
ApaI G_GGCC'C

Cuts at:    355    1269     355 
   Size:        914    3543
 
ApaLI G'TGCA_C

Cuts at:   3227    3227 
   Size:       4457
 
ApoI r'AATT_y

Cuts at:   1461    3810    4066    1461 
   Size:       2349     256    1852

  Fragments arranged by size: 

                2349    1852     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4457
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4457
 
AvaI C'yCGr_G

Cuts at:   1477    1477 
   Size:       4457
 
BamHI G'GATC_C

Cuts at:    488     719    1482     488 
   Size:        231     763    3463

  Fragments arranged by size: 

                3463     763     231
 
BanI G'GyrC_C

Cuts at:   1473    1887    2135    2170    1473 
   Size:        414     248      35    3760

  Fragments arranged by size: 

                3760     414     248      35
 
BanII G_rGCy'C

Cuts at:    355     566    1269    1471    2501     355 
   Size:        211     703     202    1030    2311

  Fragments arranged by size: 

                2311    1030     703     211     202
 
BbeI G_GCGC'C

Cuts at:   1891    2139    1891 
   Size:        248    4209
 
BciVI GTATCCnnnnn_n'

Cuts at:   2355    3116    4457    2355 
   Size:        761    1341    2355

  Fragments arranged by size: 

                2355    1341     761
 
BclI T'GATC_A

Cuts at:   1178    1977    1178 
   Size:        799    3658
 
BglI GCCn_nnn'nGGC

Cuts at:    634    1201    1442     634 
   Size:        567     241    3649

  Fragments arranged by size: 

                3649     567     241
 
BglII A'GATC_T

Cuts at:   1972    4009    1972 
   Size:       2037    2420
 
BlpI GC'TnA_GC

Cuts at:   1569    1569 
   Size:       4457
 
Bme1580I G_kGCm'C

Cuts at:    355    1269    1373    1388    2082    2175    3231     355 
   Size:        914     104      15     694      93    1056    1581

  Fragments arranged by size: 

                1581    1056     914     694     104      93      15
 
BmgBI CAC'GTC

Cuts at:    954     954 
   Size:       4457
 
BmrI ACTGGGnnnn_n'

Cuts at:   1669    1750    2085    4229    1669 
   Size:         81     335    2144    1897

  Fragments arranged by size: 

                2144    1897     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2824    2824 
   Size:       4457
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    478     499     550     775     922    1093    1243    1294    1327 
   Size:         21      51     225     147     171     150      51      33

Cuts at:   1327     478 
   Size:       3608

  Fragments arranged by size: 

                3608     225     171     150     147      51      51      33
                  21
 
Bpu10I CC'TnA_GC

Cuts at:    206     206 
   Size:       4457
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    136    1633    2512    3004    3302     136 
   Size:       1497     879     492     298    1291

  Fragments arranged by size: 

                1497    1291     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4161    4161 
   Size:       4457
 
BsaAI yAC'GTr

Cuts at:   1292    1810    1292 
   Size:        518    3939
 
BsaBI GATnn'nnATC

Cuts at:      6    1971    4008       6 
   Size:       1965    2037     455

  Fragments arranged by size: 

                2037    1965     455
 
BsaHI Gr'CG_yC

Cuts at:   1259    1888    2136    2838    4334    1259 
   Size:        629     248     702    1496    1382

  Fragments arranged by size: 

                1496    1382     702     629     248
 
BsaWI w'CCGG_w

Cuts at:   1295    1646    2167    2845    3119    3266    3677    1295 
   Size:        351     521     678     274     147     411    2075

  Fragments arranged by size: 

                2075     678     521     411     351     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    510     540     775     805     898     928     949     979    1327 
   Size:         30     235      30      93      30      21      30     348

Cuts at:   1327    1357     510 
   Size:         30    3610

  Fragments arranged by size: 

                3610     348     235      93      30      30      30      30
                  30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    574     796     826     919     997     574 
   Size:        222      30      93      78    4034

  Fragments arranged by size: 

                4034     222      93      78      30
 
BseYI C'CCAG_C

Cuts at:   1091    1154    1196    1882    2640    3217    3713    1091 
   Size:         63      42     686     758     577     496    1835

  Fragments arranged by size: 

                1835     758     686     577     496      63      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    857    1341     857 
   Size:        484    3973
 
BsiEI CG_ry'CG

Cuts at:    104     423    1999    2045    3253     104 
   Size:        319    1576      46    1208    1308

  Fragments arranged by size: 

                1576    1308    1208     319      46
 
BsiHKAI G_wGCw'C

Cuts at:    518     566    1471    2249    2439    3231     518 
   Size:         48     905     778     190     792    1744

  Fragments arranged by size: 

                1744     905     792     778     190      48
 
BsmI GAATG_Cn'

Cuts at:    474     603     474 
   Size:        129    4328
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1047    1047 
   Size:       4457
 
Bsp1286I G_dGCh'C

Cuts at:    355     518     566    1269    1373    1388    1471    2082    2175 
   Size:        163      48     703     104      15      83     611      93

Cuts at:   2175    2249    2439    2501    3231     355 
   Size:         74     190      62     730    1581

  Fragments arranged by size: 

                1581     730     703     611     190     163     104      93
                  83      74      62      48      15
 
BspEI T'CCGG_A

Cuts at:   3677    3677 
   Size:       4457
 
BspHI T'CATG_A

Cuts at:   3633    3633 
   Size:       4457
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1507    1721    2023    2404    1507 
   Size:        214     302     381    3560

  Fragments arranged by size: 

                3560     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1227    2749    2803    4287    4452      50 
   Size:       1177    1522      54    1484     165      55

  Fragments arranged by size: 

                1522    1484    1177     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    396    2369     396 
   Size:       1973    2484
 
BsrFI r'CCGG_y

Cuts at:    200    2455    2636    2845     200 
   Size:       2255     181     209    1812

  Fragments arranged by size: 

                2255    1812     209     181
 
BssSI C'ACGA_G

Cuts at:   2728    3086    2728 
   Size:        358    4099
 
BstAPI GCAn_nnn'nTGC

Cuts at:    598    1424    1834     598 
   Size:        826     410    3221

  Fragments arranged by size: 

                3221     826     410
 
BstBI TT'CG_AA

Cuts at:   2818    2818 
   Size:       4457
 
BstXI CCAn_nnnn'nTGG

Cuts at:    900     900 
   Size:       4457
 
BstYI r'GATC_y

Cuts at:      1     488     719    1208    1482    1949    1972    2307    2553 
   Size:        487     231     489     274     467      23     335     246

Cuts at:   2553    3565    3651    3663    4009       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     489     487     467     449     346     335     274
                 246     231      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1102    1102 
   Size:       4457
 
BtgI C'CryG_G

Cuts at:    236     347     929     236 
   Size:        111     582    3764

  Fragments arranged by size: 

                3764     582     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2407    2588    2751    2407 
   Size:        181     163    4113

  Fragments arranged by size: 

                4113     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1177    1700    1177 
   Size:        523    3934
 
DraI TTT'AAA

Cuts at:   1456    1456 
   Size:       4457
 
DraIII CAC_nnn'GTG

Cuts at:    740     740 
   Size:       4457
 
DrdI GACnn_nn'nnGTC

Cuts at:   2163    3021    2163 
   Size:        858    3599
 
EaeI y'GGCC_r

Cuts at:    277     428     674    1001    2042    2216    2607    2634    3979 
   Size:        151     246     327    1041     174     391      27    1345

Cuts at:   3979     277 
   Size:        755

  Fragments arranged by size: 

                1345    1041     755     391     327     246     174     151
                  27
 
EagI C'GGCC_G

Cuts at:   2042    2042 
   Size:       4457
 
EarI CTCTTCn'nnn_

Cuts at:   2480    2690    2480 
   Size:        210    4247
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2665    2975    3121    4267    2665 
   Size:        310     146    1146    2855

  Fragments arranged by size: 

                2855    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    564    1469     564 
   Size:        905    3552
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    478     499     550     633     775     796     922    1006    1093 
   Size:         21      51      83     142      21     126      84      87

Cuts at:   1093    1243    1294    1327    2282    2714    3461    3729     478 
   Size:        150      51      33     955     432     747     268    1206

  Fragments arranged by size: 

                1206     955     747     432     268     150     142     126
                  87      84      83      51      51      33      21      21
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    778     778 
   Size:       4457
 
EcoO109I rG'GnC_Cy

Cuts at:    352     377    1596     352 
   Size:         25    1219    3213

  Fragments arranged by size: 

                3213    1219      25
 
EcoRI G'AATT_C

Cuts at:   1461    1461 
   Size:       4457
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2476    2476 
   Size:       4457
 
FspI TGC'GCA

Cuts at:    715    2238     715 
   Size:       1523    2934
 
HaeII r_GCGC'y

Cuts at:   1891    2139    3161    4140    1891 
   Size:        248    1022     979    2208

  Fragments arranged by size: 

                2208    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    523     555     900     932    1046    1078    2299    2329    2331 
   Size:         32     345      32     114      32    1221      30       2

Cuts at:   2331    2361     523 
   Size:         30    2619

  Fragments arranged by size: 

                2619    1221     345     114      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1065    1496    1065 
   Size:        431    4026
 
KasI G'GCGC_C

Cuts at:   1887    2135    1887 
   Size:        248    4209
 
KpnI G_GTAC'C

Cuts at:   1477    1477 
   Size:       4457
 
MluI A'CGCG_T

Cuts at:   3890    3890 
   Size:       4457
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3128    3312    3128 
   Size:        184    4273
 
MscI TGG'CCA

Cuts at:    430     676    1003    2218     430 
   Size:        246     327    1215    2669

  Fragments arranged by size: 

                2669    1215     327     246
 
MslI CAynn'nnrTG

Cuts at:    862    1366    2446    2573     862 
   Size:        504    1080     127    2746

  Fragments arranged by size: 

                2746    1080     504     127
 
MspA1I CmG'CkG

Cuts at:    188     287     362     456     687    1158    1200    1568    1882 
   Size:         99      75      94     231     471      42     368     314

Cuts at:   1882    2242    3255    3500     188 
   Size:        360    1013     245    1145

  Fragments arranged by size: 

                1145    1013     471     368     360     314     245     231
                  99      94      75      42
 
NaeI GCC'GGC

Cuts at:    202    2638     202 
   Size:       2436    2021
 
NarI GG'CG_CC

Cuts at:   1888    2136    1888 
   Size:        248    4209
 
NcoI C'CATG_G

Cuts at:    236     347     929     236 
   Size:        111     582    3764

  Fragments arranged by size: 

                3764     582     111
 
NgoMIV G'CCGG_C

Cuts at:    200    2636     200 
   Size:       2436    2021
 
NspI r_CATG'y

Cuts at:    245    1422    1510    2010    2917     245 
   Size:       1177      88     500     907    1785

  Fragments arranged by size: 

                1785    1177     907     500      88
 
PasI CC'CwG_GG

Cuts at:   1440    1440 
   Size:       4457
 
PciI A'CATG_T

Cuts at:   2913    2913 
   Size:       4457
 
PflMI CCAn_nnn'nTGG

Cuts at:    237     999     237 
   Size:        762    3695
 
PfoI T'CCnGG_A

Cuts at:    722     722 
   Size:       4457
 
PmeI GTTT'AAAC

Cuts at:   1456    1456 
   Size:       4457
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    949     981    3596    3628     949 
   Size:         32    2615      32    1778

  Fragments arranged by size: 

                2615    1778      32      32
 
PspOMI G'GGCC_C

Cuts at:    351    1265     351 
   Size:        914    3543
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1225    1257    1225 
   Size:         32    4425
 
PstI C_TGCA'G

Cuts at:   1088    1504    2189    1088 
   Size:        416     685    3356

  Fragments arranged by size: 

                3356     685     416
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4457
 
PvuII CAG'CTG

Cuts at:    287     362     456     687    1158    1200    1882    2242     287 
   Size:         75      94     231     471      42     682     360    2502

  Fragments arranged by size: 

                2502     682     471     360     231      94      75      42
            
 
RsrII CG'GwC_CG

Cuts at:   2652    2652 
   Size:       4457
 
SacI G_AGCT'C

Cuts at:    566    1471     566 
   Size:        905    3552
 
SalI G'TCGA_C

Cuts at:   1494    1494 
   Size:       4457
 
SapI GCTCTTCn'nnn_

Cuts at:   2480    2690    2480 
   Size:        210    4247
 
SbfI CC_TGCA'GG

Cuts at:   1504    1504 
   Size:       4457
 
ScaI AGT'ACT

Cuts at:   3974    3974 
   Size:       4457
 
SexAI A'CCwGG_T

Cuts at:   1244    1244 
   Size:       4457
 
SfcI C'TryA_G

Cuts at:     33    1084    1146    1500    2185    3178    3369      33 
   Size:       1051      62     354     685     993     191    1121

  Fragments arranged by size: 

                1121    1051     993     685     354     191      62
 
SfoI GGC'GCC

Cuts at:   1889    2137    1889 
   Size:        248    4209
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4457
 
SmaI CCC'GGG

Cuts at:   1479    1479 
   Size:       4457
 
SmlI C'TyrA_G

Cuts at:    151    1612    2527    3019    3281     151 
   Size:       1461     915     492     262    1327

  Fragments arranged by size: 

                1461    1327     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4457
 
SphI G_CATG'C

Cuts at:    245    1422    1510    2010     245 
   Size:       1177      88     500    2692

  Fragments arranged by size: 

                2692    1177     500      88
 
StuI AGG'CCT

Cuts at:    499     499 
   Size:       4457
 
StyI C'CwwG_G

Cuts at:    236     347     587     929    1591    1945     236 
   Size:        111     240     342     662     354    2748

  Fragments arranged by size: 

                2748     662     354     342     240     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    165     243    2040    2173    2632    2841     165 
   Size:         78    1797     133     459     209    1781

  Fragments arranged by size: 

                1797    1781     459     209     133      78
 
TatI w'GTAC_w

Cuts at:   3972    3972 
   Size:       4457
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    510     542     775     807     898     930    3974    4006     510 
   Size:         32     233      32      91      32    3044      32     961

  Fragments arranged by size: 

                3044     961     233      91      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2254    2254 
   Size:       4457
 
XbaI T'CTAG_A

Cuts at:   1488    1488 
   Size:       4457
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    328     684    1952     328 
   Size:        356    1268    2833

  Fragments arranged by size: 

                2833    1268     356
 
XmaI C'CCGG_G

Cuts at:   1477    1477 
   Size:       4457
 
ZraI GAC'GTC

Cuts at:   1260    1260 
   Size:       4457
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AscI    AvrII     BaeI     BbsI    BbvCI
     BcgI    BfrBI     BmtI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmlI    PpuMI
    PshAI     PsiI    PspXI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI
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