(Circular) (Six-base) MAPSORT of: pF1KE0564.seq Check: 9739 from: 1 to: 4457 pF1KE0564 4457 bp With 182 enzymes: SgfI * April 24, 2009 13:16 .. AatII G_ACGT'C Cuts at: 1262 1262 Size: 4457 AccI GT'mk_AC Cuts at: 1495 1495 Size: 4457 Acc65I G'GTAC_C Cuts at: 1473 1473 Size: 4457 AclI AA'CG_TT Cuts at: 1187 4297 1187 Size: 3110 1347 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 633 796 1006 2282 2714 3461 3729 633 Size: 163 210 1276 432 747 268 1361 Fragments arranged by size: 1361 1276 747 432 268 210 163 AflIII A'CryG_T Cuts at: 1289 1809 2913 3890 1289 Size: 520 1104 977 1856 Fragments arranged by size: 1856 1104 977 520 AgeI A'CCGG_T Cuts at: 2845 2845 Size: 4457 AhdI GACnn_n'nnGTC Cuts at: 537 1060 537 Size: 523 3934 AleI CACnn'nnGTG Cuts at: 1366 1366 Size: 4457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 485 485 Size: 4457 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 949 981 949 Size: 32 4425 AlwNI CAG_nnn'CTG Cuts at: 317 1251 1762 3329 317 Size: 934 511 1567 1445 Fragments arranged by size: 1567 1445 934 511 ApaI G_GGCC'C Cuts at: 355 1269 355 Size: 914 3543 ApaLI G'TGCA_C Cuts at: 3227 3227 Size: 4457 ApoI r'AATT_y Cuts at: 1461 3810 4066 1461 Size: 2349 256 1852 Fragments arranged by size: 2349 1852 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4457 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4457 AvaI C'yCGr_G Cuts at: 1477 1477 Size: 4457 BamHI G'GATC_C Cuts at: 488 719 1482 488 Size: 231 763 3463 Fragments arranged by size: 3463 763 231 BanI G'GyrC_C Cuts at: 1473 1887 2135 2170 1473 Size: 414 248 35 3760 Fragments arranged by size: 3760 414 248 35 BanII G_rGCy'C Cuts at: 355 566 1269 1471 2501 355 Size: 211 703 202 1030 2311 Fragments arranged by size: 2311 1030 703 211 202 BbeI G_GCGC'C Cuts at: 1891 2139 1891 Size: 248 4209 BciVI GTATCCnnnnn_n' Cuts at: 2355 3116 4457 2355 Size: 761 1341 2355 Fragments arranged by size: 2355 1341 761 BclI T'GATC_A Cuts at: 1178 1977 1178 Size: 799 3658 BglI GCCn_nnn'nGGC Cuts at: 634 1201 1442 634 Size: 567 241 3649 Fragments arranged by size: 3649 567 241 BglII A'GATC_T Cuts at: 1972 4009 1972 Size: 2037 2420 BlpI GC'TnA_GC Cuts at: 1569 1569 Size: 4457 Bme1580I G_kGCm'C Cuts at: 355 1269 1373 1388 2082 2175 3231 355 Size: 914 104 15 694 93 1056 1581 Fragments arranged by size: 1581 1056 914 694 104 93 15 BmgBI CAC'GTC Cuts at: 954 954 Size: 4457 BmrI ACTGGGnnnn_n' Cuts at: 1669 1750 2085 4229 1669 Size: 81 335 2144 1897 Fragments arranged by size: 2144 1897 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2824 2824 Size: 4457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 478 499 550 775 922 1093 1243 1294 1327 Size: 21 51 225 147 171 150 51 33 Cuts at: 1327 478 Size: 3608 Fragments arranged by size: 3608 225 171 150 147 51 51 33 21 Bpu10I CC'TnA_GC Cuts at: 206 206 Size: 4457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 136 1633 2512 3004 3302 136 Size: 1497 879 492 298 1291 Fragments arranged by size: 1497 1291 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4161 4161 Size: 4457 BsaAI yAC'GTr Cuts at: 1292 1810 1292 Size: 518 3939 BsaBI GATnn'nnATC Cuts at: 6 1971 4008 6 Size: 1965 2037 455 Fragments arranged by size: 2037 1965 455 BsaHI Gr'CG_yC Cuts at: 1259 1888 2136 2838 4334 1259 Size: 629 248 702 1496 1382 Fragments arranged by size: 1496 1382 702 629 248 BsaWI w'CCGG_w Cuts at: 1295 1646 2167 2845 3119 3266 3677 1295 Size: 351 521 678 274 147 411 2075 Fragments arranged by size: 2075 678 521 411 351 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 510 540 775 805 898 928 949 979 1327 Size: 30 235 30 93 30 21 30 348 Cuts at: 1327 1357 510 Size: 30 3610 Fragments arranged by size: 3610 348 235 93 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 574 796 826 919 997 574 Size: 222 30 93 78 4034 Fragments arranged by size: 4034 222 93 78 30 BseYI C'CCAG_C Cuts at: 1091 1154 1196 1882 2640 3217 3713 1091 Size: 63 42 686 758 577 496 1835 Fragments arranged by size: 1835 758 686 577 496 63 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 857 1341 857 Size: 484 3973 BsiEI CG_ry'CG Cuts at: 104 423 1999 2045 3253 104 Size: 319 1576 46 1208 1308 Fragments arranged by size: 1576 1308 1208 319 46 BsiHKAI G_wGCw'C Cuts at: 518 566 1471 2249 2439 3231 518 Size: 48 905 778 190 792 1744 Fragments arranged by size: 1744 905 792 778 190 48 BsmI GAATG_Cn' Cuts at: 474 603 474 Size: 129 4328 BsmBI CGTCTCn'nnnn_ Cuts at: 1047 1047 Size: 4457 Bsp1286I G_dGCh'C Cuts at: 355 518 566 1269 1373 1388 1471 2082 2175 Size: 163 48 703 104 15 83 611 93 Cuts at: 2175 2249 2439 2501 3231 355 Size: 74 190 62 730 1581 Fragments arranged by size: 1581 730 703 611 190 163 104 93 83 74 62 48 15 BspEI T'CCGG_A Cuts at: 3677 3677 Size: 4457 BspHI T'CATG_A Cuts at: 3633 3633 Size: 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1507 1721 2023 2404 1507 Size: 214 302 381 3560 Fragments arranged by size: 3560 381 302 214 BsrBI CCG'CTC Cuts at: 50 1227 2749 2803 4287 4452 50 Size: 1177 1522 54 1484 165 55 Fragments arranged by size: 1522 1484 1177 165 55 54 BsrDI GCAATG_nn' Cuts at: 396 2369 396 Size: 1973 2484 BsrFI r'CCGG_y Cuts at: 200 2455 2636 2845 200 Size: 2255 181 209 1812 Fragments arranged by size: 2255 1812 209 181 BssSI C'ACGA_G Cuts at: 2728 3086 2728 Size: 358 4099 BstAPI GCAn_nnn'nTGC Cuts at: 598 1424 1834 598 Size: 826 410 3221 Fragments arranged by size: 3221 826 410 BstBI TT'CG_AA Cuts at: 2818 2818 Size: 4457 BstXI CCAn_nnnn'nTGG Cuts at: 900 900 Size: 4457 BstYI r'GATC_y Cuts at: 1 488 719 1208 1482 1949 1972 2307 2553 Size: 487 231 489 274 467 23 335 246 Cuts at: 2553 3565 3651 3663 4009 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 489 487 467 449 346 335 274 246 231 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1102 1102 Size: 4457 BtgI C'CryG_G Cuts at: 236 347 929 236 Size: 111 582 3764 Fragments arranged by size: 3764 582 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2407 2588 2751 2407 Size: 181 163 4113 Fragments arranged by size: 4113 181 163 BtsI GCAGTG_nn' Cuts at: 1177 1700 1177 Size: 523 3934 DraI TTT'AAA Cuts at: 1456 1456 Size: 4457 DraIII CAC_nnn'GTG Cuts at: 740 740 Size: 4457 DrdI GACnn_nn'nnGTC Cuts at: 2163 3021 2163 Size: 858 3599 EaeI y'GGCC_r Cuts at: 277 428 674 1001 2042 2216 2607 2634 3979 Size: 151 246 327 1041 174 391 27 1345 Cuts at: 3979 277 Size: 755 Fragments arranged by size: 1345 1041 755 391 327 246 174 151 27 EagI C'GGCC_G Cuts at: 2042 2042 Size: 4457 EarI CTCTTCn'nnn_ Cuts at: 2480 2690 2480 Size: 210 4247 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2665 2975 3121 4267 2665 Size: 310 146 1146 2855 Fragments arranged by size: 2855 1146 310 146 EcoICRI GAG'CTC Cuts at: 564 1469 564 Size: 905 3552 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 478 499 550 633 775 796 922 1006 1093 Size: 21 51 83 142 21 126 84 87 Cuts at: 1093 1243 1294 1327 2282 2714 3461 3729 478 Size: 150 51 33 955 432 747 268 1206 Fragments arranged by size: 1206 955 747 432 268 150 142 126 87 84 83 51 51 33 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 778 778 Size: 4457 EcoO109I rG'GnC_Cy Cuts at: 352 377 1596 352 Size: 25 1219 3213 Fragments arranged by size: 3213 1219 25 EcoRI G'AATT_C Cuts at: 1461 1461 Size: 4457 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 4457 FspI TGC'GCA Cuts at: 715 2238 715 Size: 1523 2934 HaeII r_GCGC'y Cuts at: 1891 2139 3161 4140 1891 Size: 248 1022 979 2208 Fragments arranged by size: 2208 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 523 555 900 932 1046 1078 2299 2329 2331 Size: 32 345 32 114 32 1221 30 2 Cuts at: 2331 2361 523 Size: 30 2619 Fragments arranged by size: 2619 1221 345 114 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1065 1496 1065 Size: 431 4026 KasI G'GCGC_C Cuts at: 1887 2135 1887 Size: 248 4209 KpnI G_GTAC'C Cuts at: 1477 1477 Size: 4457 MluI A'CGCG_T Cuts at: 3890 3890 Size: 4457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3128 3312 3128 Size: 184 4273 MscI TGG'CCA Cuts at: 430 676 1003 2218 430 Size: 246 327 1215 2669 Fragments arranged by size: 2669 1215 327 246 MslI CAynn'nnrTG Cuts at: 862 1366 2446 2573 862 Size: 504 1080 127 2746 Fragments arranged by size: 2746 1080 504 127 MspA1I CmG'CkG Cuts at: 188 287 362 456 687 1158 1200 1568 1882 Size: 99 75 94 231 471 42 368 314 Cuts at: 1882 2242 3255 3500 188 Size: 360 1013 245 1145 Fragments arranged by size: 1145 1013 471 368 360 314 245 231 99 94 75 42 NaeI GCC'GGC Cuts at: 202 2638 202 Size: 2436 2021 NarI GG'CG_CC Cuts at: 1888 2136 1888 Size: 248 4209 NcoI C'CATG_G Cuts at: 236 347 929 236 Size: 111 582 3764 Fragments arranged by size: 3764 582 111 NgoMIV G'CCGG_C Cuts at: 200 2636 200 Size: 2436 2021 NspI r_CATG'y Cuts at: 245 1422 1510 2010 2917 245 Size: 1177 88 500 907 1785 Fragments arranged by size: 1785 1177 907 500 88 PasI CC'CwG_GG Cuts at: 1440 1440 Size: 4457 PciI A'CATG_T Cuts at: 2913 2913 Size: 4457 PflMI CCAn_nnn'nTGG Cuts at: 237 999 237 Size: 762 3695 PfoI T'CCnGG_A Cuts at: 722 722 Size: 4457 PmeI GTTT'AAAC Cuts at: 1456 1456 Size: 4457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 949 981 3596 3628 949 Size: 32 2615 32 1778 Fragments arranged by size: 2615 1778 32 32 PspOMI G'GGCC_C Cuts at: 351 1265 351 Size: 914 3543 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1225 1257 1225 Size: 32 4425 PstI C_TGCA'G Cuts at: 1088 1504 2189 1088 Size: 416 685 3356 Fragments arranged by size: 3356 685 416 PvuI CG_AT'CG Cuts at: 104 104 Size: 4457 PvuII CAG'CTG Cuts at: 287 362 456 687 1158 1200 1882 2242 287 Size: 75 94 231 471 42 682 360 2502 Fragments arranged by size: 2502 682 471 360 231 94 75 42 RsrII CG'GwC_CG Cuts at: 2652 2652 Size: 4457 SacI G_AGCT'C Cuts at: 566 1471 566 Size: 905 3552 SalI G'TCGA_C Cuts at: 1494 1494 Size: 4457 SapI GCTCTTCn'nnn_ Cuts at: 2480 2690 2480 Size: 210 4247 SbfI CC_TGCA'GG Cuts at: 1504 1504 Size: 4457 ScaI AGT'ACT Cuts at: 3974 3974 Size: 4457 SexAI A'CCwGG_T Cuts at: 1244 1244 Size: 4457 SfcI C'TryA_G Cuts at: 33 1084 1146 1500 2185 3178 3369 33 Size: 1051 62 354 685 993 191 1121 Fragments arranged by size: 1121 1051 993 685 354 191 62 SfoI GGC'GCC Cuts at: 1889 2137 1889 Size: 248 4209 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4457 SmaI CCC'GGG Cuts at: 1479 1479 Size: 4457 SmlI C'TyrA_G Cuts at: 151 1612 2527 3019 3281 151 Size: 1461 915 492 262 1327 Fragments arranged by size: 1461 1327 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4457 SphI G_CATG'C Cuts at: 245 1422 1510 2010 245 Size: 1177 88 500 2692 Fragments arranged by size: 2692 1177 500 88 StuI AGG'CCT Cuts at: 499 499 Size: 4457 StyI C'CwwG_G Cuts at: 236 347 587 929 1591 1945 236 Size: 111 240 342 662 354 2748 Fragments arranged by size: 2748 662 354 342 240 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 165 243 2040 2173 2632 2841 165 Size: 78 1797 133 459 209 1781 Fragments arranged by size: 1797 1781 459 209 133 78 TatI w'GTAC_w Cuts at: 3972 3972 Size: 4457 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 510 542 775 807 898 930 3974 4006 510 Size: 32 233 32 91 32 3044 32 961 Fragments arranged by size: 3044 961 233 91 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2254 2254 Size: 4457 XbaI T'CTAG_A Cuts at: 1488 1488 Size: 4457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 328 684 1952 328 Size: 356 1268 2833 Fragments arranged by size: 2833 1268 356 XmaI C'CCGG_G Cuts at: 1477 1477 Size: 4457 ZraI GAC'GTC Cuts at: 1260 1260 Size: 4457 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI