Restriction map for
pF1KE0565
(Circular) (Six-base) MAPSORT of: pF1KE0565.seq Check: 7074 from: 1 to: 3827
pF1KE0565 3827 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 865 865
Size: 3827
Acc65I G'GTAC_C
Cuts at: 843 843
Size: 3827
AclI AA'CG_TT
Cuts at: 3667 3667
Size: 3827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2084 2831 3099 1652
Size: 432 747 268 2380
Fragments arranged by size:
2380 747 432 268
AflIII A'CryG_T
Cuts at: 1179 2283 3260 1179
Size: 1104 977 1746
Fragments arranged by size:
1746 1104 977
AgeI A'CCGG_T
Cuts at: 2215 2215
Size: 3827
AlwNI CAG_nnn'CTG
Cuts at: 380 557 587 617 674 704 761 791 1132
Size: 177 30 30 57 30 57 30 341
Cuts at: 1132 2699 380
Size: 1567 1508
Fragments arranged by size:
1567 1508 341 177 57 57 30 30
30 30
ApaLI G'TGCA_C
Cuts at: 2597 2597
Size: 3827
ApoI r'AATT_y
Cuts at: 831 3180 3436 831
Size: 2349 256 1222
Fragments arranged by size:
2349 1222 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3827
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
AvaI C'yCGr_G
Cuts at: 847 847
Size: 3827
BamHI G'GATC_C
Cuts at: 852 852
Size: 3827
BanI G'GyrC_C
Cuts at: 843 1257 1505 1540 843
Size: 414 248 35 3130
Fragments arranged by size:
3130 414 248 35
BanII G_rGCy'C
Cuts at: 295 457 841 1871 295
Size: 162 384 1030 2251
Fragments arranged by size:
2251 1030 384 162
BbeI G_GCGC'C
Cuts at: 1261 1509 1261
Size: 248 3579
BciVI GTATCCnnnnn_n'
Cuts at: 1725 2486 3827 1725
Size: 761 1341 1725
Fragments arranged by size:
1725 1341 761
BclI T'GATC_A
Cuts at: 1347 1347
Size: 3827
BglII A'GATC_T
Cuts at: 1342 3379 1342
Size: 2037 1790
BlpI GC'TnA_GC
Cuts at: 939 939
Size: 3827
Bme1580I G_kGCm'C
Cuts at: 389 1452 1545 2601 389
Size: 1063 93 1056 1615
Fragments arranged by size:
1615 1063 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 483 570 600 687 774 1039 1120 1455 3599
Size: 87 30 87 87 265 81 335 2144
Cuts at: 3599 483
Size: 711
Fragments arranged by size:
2144 711 335 265 87 87 87 81
30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 3827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 147 149 255 357 387 126
Size: 21 2 106 102 30 3566
Fragments arranged by size:
3566 106 102 30 21 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1882 2374 2672 1003
Size: 879 492 298 2158
Fragments arranged by size:
2158 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 3827
BsaAI yAC'GTr
Cuts at: 1180 1180
Size: 3827
BsaBI GATnn'nnATC
Cuts at: 6 1341 3378 6
Size: 1335 2037 455
Fragments arranged by size:
2037 1335 455
BsaHI Gr'CG_yC
Cuts at: 1258 1506 2208 3704 1258
Size: 248 702 1496 1381
Fragments arranged by size:
1496 1381 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1537 2215 2489 2636 3047 1016
Size: 521 678 274 147 411 1796
Fragments arranged by size:
1796 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 390 510 627 714 765 390
Size: 120 117 87 51 3452
Fragments arranged by size:
3452 120 117 87 51
BseYI C'CCAG_C
Cuts at: 565 1252 2010 2587 3083 565
Size: 687 758 577 496 1309
Fragments arranged by size:
1309 758 687 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 513
Size: 3827
BsiEI CG_ry'CG
Cuts at: 104 1369 1415 2623 104
Size: 1265 46 1208 1308
Fragments arranged by size:
1308 1265 1208 46
BsiHKAI G_wGCw'C
Cuts at: 841 1619 1809 2601 841
Size: 778 190 792 2067
Fragments arranged by size:
2067 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 295 389 457 841 1452 1545 1619 1809 1871
Size: 94 68 384 611 93 74 190 62
Cuts at: 1871 2601 295
Size: 730 1521
Fragments arranged by size:
1521 730 611 384 190 94 93 74
68 62
BspEI T'CCGG_A
Cuts at: 3047 3047
Size: 3827
BspHI T'CATG_A
Cuts at: 3003 3003
Size: 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 877 1091 1393 1774 877
Size: 214 302 381 2930
Fragments arranged by size:
2930 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2119 2173 3657 3822 50
Size: 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1739 1739
Size: 3827
BsrFI r'CCGG_y
Cuts at: 1825 2006 2215 1825
Size: 181 209 3437
Fragments arranged by size:
3437 209 181
BssSI C'ACGA_G
Cuts at: 2098 2456 2098
Size: 358 3469
BstAPI GCAn_nnn'nTGC
Cuts at: 683 770 1204 683
Size: 87 434 3306
Fragments arranged by size:
3306 434 87
BstBI TT'CG_AA
Cuts at: 2188 2188
Size: 3827
BstYI r'GATC_y
Cuts at: 1 852 1319 1342 1677 1923 2935 3021 3033
Size: 851 467 23 335 246 1012 86 12
Cuts at: 3033 3379 1
Size: 346 449
Fragments arranged by size:
1012 851 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 522 639 726 522
Size: 117 87 3623
Fragments arranged by size:
3623 117 87
BtgI C'CryG_G
Cuts at: 107 107
Size: 3827
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1777 1958 2121 1777
Size: 181 163 3483
Fragments arranged by size:
3483 181 163
BtsI GCAGTG_nn'
Cuts at: 1070 1070
Size: 3827
DraI TTT'AAA
Cuts at: 826 826
Size: 3827
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2391 1533
Size: 858 2969
EaeI y'GGCC_r
Cuts at: 1412 1586 1977 2004 3349 1412
Size: 174 391 27 1345 1890
Fragments arranged by size:
1890 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1412 1412
Size: 3827
EarI CTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2035 2345 2491 3637 2035
Size: 310 146 1146 2225
Fragments arranged by size:
2225 1146 310 146
EcoICRI GAG'CTC
Cuts at: 839 839
Size: 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 147 149 255 357 387 1652 2084 2831
Size: 21 2 106 102 30 1265 432 747
Cuts at: 2831 3099 126
Size: 268 854
Fragments arranged by size:
1265 854 747 432 268 106 102 30
21 2
EcoO109I rG'GnC_Cy
Cuts at: 966 966
Size: 3827
EcoRI G'AATT_C
Cuts at: 831 831
Size: 3827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3827
FspI TGC'GCA
Cuts at: 1608 1608
Size: 3827
HaeII r_GCGC'y
Cuts at: 1261 1509 2531 3510 1261
Size: 248 1022 979 1578
Fragments arranged by size:
1578 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1669 1699 1701 1731 1669
Size: 30 2 30 3765
Fragments arranged by size:
3765 30 30 2
HincII GTy'rAC
Cuts at: 866 866
Size: 3827
KasI G'GCGC_C
Cuts at: 1257 1505 1257
Size: 248 3579
KpnI G_GTAC'C
Cuts at: 847 847
Size: 3827
MluI A'CGCG_T
Cuts at: 3260 3260
Size: 3827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2682 2498
Size: 184 3643
MscI TGG'CCA
Cuts at: 1588 1588
Size: 3827
MslI CAynn'nnrTG
Cuts at: 1816 1943 1816
Size: 127 3700
MspA1I CmG'CkG
Cuts at: 146 167 275 377 407 497 554 569 584
Size: 21 108 102 30 90 57 15 15
Cuts at: 584 614 671 701 758 788 938 1252 1612
Size: 30 57 30 57 30 150 314 360
Cuts at: 1612 2625 2870 146
Size: 1013 245 1103
Fragments arranged by size:
1103 1013 360 314 245 150 108 102
90 57 57 57 30 30 30 30
21 15 15
NaeI GCC'GGC
Cuts at: 2008 2008
Size: 3827
NarI GG'CG_CC
Cuts at: 1258 1506 1258
Size: 248 3579
NcoI C'CATG_G
Cuts at: 107 107
Size: 3827
NgoMIV G'CCGG_C
Cuts at: 2006 2006
Size: 3827
NspI r_CATG'y
Cuts at: 880 1380 2287 880
Size: 500 907 2420
Fragments arranged by size:
2420 907 500
PciI A'CATG_T
Cuts at: 2283 2283
Size: 3827
PmeI GTTT'AAAC
Cuts at: 826 826
Size: 3827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 3795
PstI C_TGCA'G
Cuts at: 874 1559 874
Size: 685 3142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3827
PvuII CAG'CTG
Cuts at: 146 167 275 377 407 497 554 569 584
Size: 21 108 102 30 90 57 15 15
Cuts at: 584 614 671 701 758 788 1252 1612 146
Size: 30 57 30 57 30 464 360 2361
Fragments arranged by size:
2361 464 360 108 102 90 57 57
57 30 30 30 30 21 15 15
RsrII CG'GwC_CG
Cuts at: 2022 2022
Size: 3827
SacI G_AGCT'C
Cuts at: 841 841
Size: 3827
SalI G'TCGA_C
Cuts at: 864 864
Size: 3827
SapI GCTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
SbfI CC_TGCA'GG
Cuts at: 874 874
Size: 3827
ScaI AGT'ACT
Cuts at: 3344 3344
Size: 3827
SfcI C'TryA_G
Cuts at: 33 870 1555 2548 2739 33
Size: 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 191
SfoI GGC'GCC
Cuts at: 1259 1507 1259
Size: 248 3579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
SmaI CCC'GGG
Cuts at: 849 849
Size: 3827
SmlI C'TyrA_G
Cuts at: 982 1897 2389 2651 982
Size: 915 492 262 2158
Fragments arranged by size:
2158 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3827
SphI G_CATG'C
Cuts at: 880 1380 880
Size: 500 3327
StyI C'CwwG_G
Cuts at: 107 296 356 428 458 961 1315 107
Size: 189 60 72 30 503 354 2619
Fragments arranged by size:
2619 503 354 189 72 60 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 1543 2002 2211 1410
Size: 133 459 209 3026
Fragments arranged by size:
3026 459 209 133
TatI w'GTAC_w
Cuts at: 3342 3342
Size: 3827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 3795
Tth111I GACn'n_nGTC
Cuts at: 1624 1624
Size: 3827
XbaI T'CTAG_A
Cuts at: 858 858
Size: 3827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1322 1322
Size: 3827
XmaI C'CCGG_G
Cuts at: 847 847
Size: 3827
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI