Restriction map for
pF1KE0567
(Circular) (Six-base) MAPSORT of: pF1KE0567.seq Check: 3338 from: 1 to: 3878
pF1KE0567 3878 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 150 577 150
Size: 427 3451
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1703 2135 2882 3150 1703
Size: 432 747 268 2431
Fragments arranged by size:
2431 747 432 268
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 143 215 275 305 329 1183 2750 143
Size: 72 60 30 24 854 1567 1271
Fragments arranged by size:
1567 1271 854 72 60 30 24
ApaLI G'TGCA_C
Cuts at: 2648 2648
Size: 3878
ApoI r'AATT_y
Cuts at: 882 3231 3487 882
Size: 2349 256 1273
Fragments arranged by size:
2349 1273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 898 898
Size: 3878
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 202 894 1308 1556 1591 202
Size: 692 414 248 35 2489
Fragments arranged by size:
2489 692 414 248 35
BanII G_rGCy'C
Cuts at: 194 299 892 1922 194
Size: 105 593 1030 2150
Fragments arranged by size:
2150 1030 593 105
BbeI G_GCGC'C
Cuts at: 1312 1560 1312
Size: 248 3630
BciVI GTATCCnnnnn_n'
Cuts at: 1776 2537 3878 1776
Size: 761 1341 1776
Fragments arranged by size:
1776 1341 761
BclI T'GATC_A
Cuts at: 1398 1398
Size: 3878
BglI GCCn_nnn'nGGC
Cuts at: 314 314
Size: 3878
BglII A'GATC_T
Cuts at: 1393 3430 1393
Size: 2037 1841
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 222 413 443 566 1503 1596 2652 222
Size: 191 30 123 937 93 1056 1448
Fragments arranged by size:
1448 1056 937 191 123 93 30
BmrI ACTGGGnnnn_n'
Cuts at: 247 1090 1171 1506 3650 247
Size: 843 81 335 2144 475
Fragments arranged by size:
2144 843 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 600 2245 600
Size: 1645 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 120
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1933 2425 2723 1054
Size: 879 492 298 2209
Fragments arranged by size:
2209 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 1231 1231
Size: 3878
BsaBI GATnn'nnATC
Cuts at: 6 1392 3429 6
Size: 1386 2037 455
Fragments arranged by size:
2037 1386 455
BsaHI Gr'CG_yC
Cuts at: 1309 1557 2259 3755 1309
Size: 248 702 1496 1432
Fragments arranged by size:
1496 1432 702 248
BsaWI w'CCGG_w
Cuts at: 1067 1588 2266 2540 2687 3098 1067
Size: 521 678 274 147 411 1847
Fragments arranged by size:
1847 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 372 621 762 780 783 796 372
Size: 249 141 18 3 13 3454
Fragments arranged by size:
3454 249 141 18 13 3
BseYI C'CCAG_C
Cuts at: 208 222 298 831 1303 2061 2638 3134 208
Size: 14 76 533 472 758 577 496 952
Fragments arranged by size:
952 758 577 533 496 472 76 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 231 303 315 360 609 750 129
Size: 102 72 12 45 249 141 3257
Fragments arranged by size:
3257 249 141 102 72 45 12
BsiEI CG_ry'CG
Cuts at: 104 1420 1466 2674 104
Size: 1316 46 1208 1308
Fragments arranged by size:
1316 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 826 892 1670 1860 2652 194
Size: 632 66 778 190 792 1420
Fragments arranged by size:
1420 792 778 632 190 66
Bsp1286I G_dGCh'C
Cuts at: 194 222 299 413 443 566 826 892 1503
Size: 28 77 114 30 123 260 66 611
Cuts at: 1503 1596 1670 1860 1922 2652 194
Size: 93 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 260 190 123 114 93
77 74 66 62 30 28
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 150 577 928 1142 1444 1825 150
Size: 427 351 214 302 381 2203
Fragments arranged by size:
2203 427 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 2170 2224 3708 3873 50
Size: 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 366 615 1876 2057 2266 366
Size: 249 1261 181 209 1978
Fragments arranged by size:
1978 1261 249 209 181
BssSI C'ACGA_G
Cuts at: 339 2149 2507 339
Size: 1810 358 1710
Fragments arranged by size:
1810 1710 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 1255
Size: 3878
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstYI r'GATC_y
Cuts at: 1 903 1370 1393 1728 1974 2986 3072 3084
Size: 902 467 23 335 246 1012 86 12
Cuts at: 3084 3430 1
Size: 346 449
Fragments arranged by size:
1012 902 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 852 852
Size: 3878
BtgI C'CryG_G
Cuts at: 107 107
Size: 3878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 1121 1121
Size: 3878
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 110 1463 1637 2028 2055 3400 110
Size: 1353 174 391 27 1345 588
Fragments arranged by size:
1353 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 510 591 1901 2111 510
Size: 81 1310 210 2277
Fragments arranged by size:
2277 1310 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2086 2396 2542 3688 2086
Size: 310 146 1146 2276
Fragments arranged by size:
2276 1146 310 146
EcoICRI GAG'CTC
Cuts at: 192 890 192
Size: 698 3180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 1703 2135 2882 3150 120
Size: 1583 432 747 268 848
Fragments arranged by size:
1583 848 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1017 1017
Size: 3878
EcoRI G'AATT_C
Cuts at: 882 882
Size: 3878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3878
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 143 305 1312 1560 2582 3561 143
Size: 162 1007 248 1022 979 460
Fragments arranged by size:
1022 1007 979 460 248 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1750 1752 1782 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 30 30 2
HincII GTy'rAC
Cuts at: 917 917
Size: 3878
KasI G'GCGC_C
Cuts at: 1308 1556 1308
Size: 248 3630
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2733 2549
Size: 184 3694
MscI TGG'CCA
Cuts at: 1639 1639
Size: 3878
MslI CAynn'nnrTG
Cuts at: 253 1867 1994 253
Size: 1614 127 2137
Fragments arranged by size:
2137 1614 127
MspA1I CmG'CkG
Cuts at: 212 989 1303 1663 2676 2921 212
Size: 777 314 360 1013 245 1169
Fragments arranged by size:
1169 1013 777 360 314 245
NaeI GCC'GGC
Cuts at: 368 617 2059 368
Size: 249 1442 2187
Fragments arranged by size:
2187 1442 249
NarI GG'CG_CC
Cuts at: 1309 1557 1309
Size: 248 3630
NcoI C'CATG_G
Cuts at: 107 107
Size: 3878
NgoMIV G'CCGG_C
Cuts at: 366 615 2057 366
Size: 249 1442 2187
Fragments arranged by size:
2187 1442 249
NspI r_CATG'y
Cuts at: 931 1431 2338 931
Size: 500 907 2471
Fragments arranged by size:
2471 907 500
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PflMI CCAn_nnn'nTGG
Cuts at: 155 155
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3846
PshAI GACnn'nnGTC
Cuts at: 241 241
Size: 3878
PstI C_TGCA'G
Cuts at: 784 844 925 1610 784
Size: 60 81 685 3052
Fragments arranged by size:
3052 685 81 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 212 1303 1663 212
Size: 1091 360 2427
Fragments arranged by size:
2427 1091 360
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 194 892 194
Size: 698 3180
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 3395 3395
Size: 3878
SfcI C'TryA_G
Cuts at: 33 780 840 921 1606 2599 2790 33
Size: 747 60 81 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 191 81 60
SfoI GGC'GCC
Cuts at: 1310 1558 1310
Size: 248 3630
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 900 900
Size: 3878
SmlI C'TyrA_G
Cuts at: 1033 1948 2440 2702 1033
Size: 915 492 262 2209
Fragments arranged by size:
2209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 931 1431 931
Size: 500 3378
StuI AGG'CCT
Cuts at: 400 400
Size: 3878
StyI C'CwwG_G
Cuts at: 107 1012 1366 107
Size: 905 354 2619
Fragments arranged by size:
2619 905 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1461 1594 2053 2262 1461
Size: 133 459 209 3077
Fragments arranged by size:
3077 459 209 133
TatI w'GTAC_w
Cuts at: 3393 3393
Size: 3878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 1675 1675
Size: 3878
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 898 898
Size: 3878
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI