Restriction map for
pF1KE0568
(Circular) (Six-base) MAPSORT of: pF1KE0568.seq Check: 375 from: 1 to: 3977
pF1KE0568 3977 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1015 1015
Size: 3977
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 1802 2234 2981 3249 716
Size: 1086 432 747 268 1444
Fragments arranged by size:
1444 1086 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 254 710 767 824 854 911 1282 2849 254
Size: 456 57 57 30 57 371 1567 1382
Fragments arranged by size:
1567 1382 456 371 57 57 57 30
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 997 997
Size: 3977
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 993 1407 1655 1690 993
Size: 414 248 35 3280
Fragments arranged by size:
3280 414 248 35
BanII G_rGCy'C
Cuts at: 337 427 487 607 667 991 2021 337
Size: 90 60 120 60 324 1030 2293
Fragments arranged by size:
2293 1030 324 120 90 60 60
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 263 1602 1695 2751 263
Size: 1339 93 1056 1489
Fragments arranged by size:
1489 1339 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 693 924 1189 1270 1605 3749 693
Size: 231 265 81 335 2144 921
Fragments arranged by size:
2144 921 335 265 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 231 297 149
Size: 82 66 3829
Fragments arranged by size:
3829 82 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 1408 1656 2358 3854 1408
Size: 248 702 1496 1531
Fragments arranged by size:
1531 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 774 777 861 864 774
Size: 3 84 3 3887
Fragments arranged by size:
3887 84 3 3
BseYI C'CCAG_C
Cuts at: 1402 2160 2737 3233 1402
Size: 758 577 496 2146
Fragments arranged by size:
2146 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 263 337 427 487 607 667 991 1602 1695
Size: 74 90 60 120 60 324 611 93
Cuts at: 1695 1769 1959 2021 2751 263
Size: 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 324 190 120 93 90
74 74 62 60 60
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1027 1241 1543 1924 1027
Size: 214 302 381 3080
Fragments arranged by size:
3080 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 1889
Size: 3977
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 920 1354 920
Size: 434 3543
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstYI r'GATC_y
Cuts at: 1 1002 1469 1492 1827 2073 3085 3171 3183
Size: 1001 467 23 335 246 1012 86 12
Cuts at: 3183 3529 1
Size: 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 789 876 789
Size: 87 3890
BtgI C'CryG_G
Cuts at: 107 107
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 1562 1736 2127 2154 3499 1562
Size: 174 391 27 1345 2040
Fragments arranged by size:
2040 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 735 2000 2210 735
Size: 1265 210 2502
Fragments arranged by size:
2502 1265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 231 297 716 1802 2234 2981 3249 149
Size: 82 66 419 1086 432 747 268 877
Fragments arranged by size:
1086 877 747 432 419 268 82 66
EcoO109I rG'GnC_Cy
Cuts at: 1116 1116
Size: 3977
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 1411 1659 2681 3660 1411
Size: 248 1022 979 1728
Fragments arranged by size:
1728 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1819 1849 1851 1881 1819
Size: 30 2 30 3915
Fragments arranged by size:
3915 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 1738 1738
Size: 3977
MslI CAynn'nnrTG
Cuts at: 1966 2093 1966
Size: 127 3850
MspA1I CmG'CkG
Cuts at: 251 317 707 764 821 851 1088 1402 1762
Size: 66 390 57 57 30 237 314 360
Cuts at: 1762 2775 3020 251
Size: 1013 245 1208
Fragments arranged by size:
1208 1013 390 360 314 245 237 66
57 57 30
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NcoI C'CATG_G
Cuts at: 107 107
Size: 3977
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 1030 1530 2437 1030
Size: 500 907 2570
Fragments arranged by size:
2570 907 500
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PstI C_TGCA'G
Cuts at: 1024 1709 1024
Size: 685 3292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 251 317 707 764 821 851 1402 1762 251
Size: 66 390 57 57 30 551 360 2466
Fragments arranged by size:
2466 551 390 360 66 57 57 30
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 735 2000 2210 735
Size: 1265 210 2502
Fragments arranged by size:
2502 1265 210
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 1020 1705 2698 2889 33
Size: 987 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 191
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 107 338 368 398 428 458 488 518 548
Size: 231 30 30 30 30 30 30 30
Cuts at: 548 578 608 638 1111 1465 107
Size: 30 30 30 473 354 2619
Fragments arranged by size:
2619 473 354 231 30 30 30 30
30 30 30 30 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1560 1693 2152 2361 1560
Size: 133 459 209 3176
Fragments arranged by size:
3176 459 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 3945
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1472 1472
Size: 3977
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI