Restriction map for
pF1KE0575
(Circular) (Six-base) MAPSORT of: pF1KE0575.seq Check: 6486 from: 1 to: 4169
pF1KE0575 4169 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1994 2426 3173 3441 460
Size: 1534 432 747 268 1188
Fragments arranged by size:
1534 1188 747 432 268
AflIII A'CryG_T
Cuts at: 237 1521 2625 3602 237
Size: 1284 1104 977 804
Fragments arranged by size:
1284 1104 977 804
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 692 973 1474 3041 692
Size: 281 501 1567 1820
Fragments arranged by size:
1820 1567 501 281
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 395 556 1173 3522 3778 395
Size: 161 617 2349 256 786
Fragments arranged by size:
2349 786 617 256 161
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
AvrII C'CTAG_G
Cuts at: 228 228
Size: 4169
BamHI G'GATC_C
Cuts at: 623 1194 623
Size: 571 3598
BanI G'GyrC_C
Cuts at: 1185 1599 1847 1882 1185
Size: 414 248 35 3472
Fragments arranged by size:
3472 414 248 35
BanII G_rGCy'C
Cuts at: 217 1183 2213 217
Size: 966 1030 2173
Fragments arranged by size:
2173 1030 966
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 755 1689 755
Size: 934 3235
BglII A'GATC_T
Cuts at: 161 1684 3721 161
Size: 1523 2037 609
Fragments arranged by size:
2037 1523 609
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 581 1794 1887 2943 581
Size: 1213 93 1056 1807
Fragments arranged by size:
1807 1213 1056 93
BmgBI CAC'GTC
Cuts at: 238 238
Size: 4169
BmrI ACTGGGnnnn_n'
Cuts at: 308 728 1021 1381 1462 1797 3941 308
Size: 420 293 360 81 335 2144 536
Fragments arranged by size:
2144 536 420 360 335 293 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 1090 2536 450
Size: 640 1446 2083
Fragments arranged by size:
2083 1446 640
Bpu10I CC'TnA_GC
Cuts at: 182 182
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1345 2224 2716 3014 1105
Size: 240 879 492 298 2260
Fragments arranged by size:
2260 879 492 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 1600 1848 2550 4046 1600
Size: 248 702 1496 1723
Fragments arranged by size:
1723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 224 254 478 503 508 533 178
Size: 30 16 30 224 25 5 25 3814
Fragments arranged by size:
3814 224 30 30 25 25 16 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1063 1063
Size: 4169
BseYI C'CCAG_C
Cuts at: 593 619 813 1594 2352 2929 3425 593
Size: 26 194 781 758 577 496 1337
Fragments arranged by size:
1337 781 758 577 496 194 26
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 183 1183 1961 2151 2943 183
Size: 1000 778 190 792 1409
Fragments arranged by size:
1409 1000 792 778 190
BsmI GAATG_Cn'
Cuts at: 588 931 588
Size: 343 3826
Bsp1286I G_dGCh'C
Cuts at: 183 217 581 1183 1794 1887 1961 2151 2213
Size: 34 364 602 611 93 74 190 62
Cuts at: 2213 2943 183
Size: 730 1409
Fragments arranged by size:
1409 730 611 602 364 190 93 74
62 34
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1219 1433 1735 2116 1147
Size: 72 214 302 381 3200
Fragments arranged by size:
3200 381 302 214 72
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 298 795 298
Size: 497 3672
BstYI r'GATC_y
Cuts at: 1 161 623 1194 1661 1684 2019 2265 3277
Size: 160 462 571 467 23 335 246 1012
Cuts at: 3277 3363 3375 3721 1
Size: 86 12 346 449
Fragments arranged by size:
1012 571 467 462 449 346 335 246
160 86 23 12
BtgI C'CryG_G
Cuts at: 107 650 107
Size: 543 3626
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 208 1754 1928 2319 2346 3691 208
Size: 1546 174 391 27 1345 686
Fragments arranged by size:
1546 1345 686 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 838 2192 2402 838
Size: 1354 210 2605
Fragments arranged by size:
2605 1354 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1994 2426 3173 3441 460
Size: 1534 432 747 268 1188
Fragments arranged by size:
1534 1188 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 302 377 655 980 1308 302
Size: 75 278 325 328 3163
Fragments arranged by size:
3163 328 325 278 75
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 1603 1851 2873 3852 1603
Size: 248 1022 979 1920
Fragments arranged by size:
1920 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 887 919 2011 2041 2043 2073 178
Size: 32 677 32 1092 30 2 30 2274
Fragments arranged by size:
2274 1092 677 32 32 30 30 2
HincII GTy'rAC
Cuts at: 827 1208 827
Size: 381 3788
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 359 372 2840 3024 359
Size: 13 2468 184 1504
Fragments arranged by size:
2468 1504 184 13
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 176 793 2158 2285 176
Size: 617 1365 127 2060
Fragments arranged by size:
2060 1365 617 127
MspA1I CmG'CkG
Cuts at: 424 1037 1280 1594 1954 2967 3212 424
Size: 613 243 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 613 360 314 245 243
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 650 107
Size: 543 3626
NdeI CA'TA_TG
Cuts at: 277 1144 277
Size: 867 3302
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 175 508 1222 1722 2629 175
Size: 333 714 500 907 1715
Fragments arranged by size:
1715 907 714 500 333
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PflMI CCAn_nnn'nTGG
Cuts at: 612 973 612
Size: 361 3808
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PpuMI rG'GwC_Cy
Cuts at: 302 377 655 980 302
Size: 75 278 325 3491
Fragments arranged by size:
3491 325 278 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 856 888 856
Size: 32 4137
PstI C_TGCA'G
Cuts at: 841 1216 1901 841
Size: 375 685 3109
Fragments arranged by size:
3109 685 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 424 1594 1954 424
Size: 1170 360 2639
Fragments arranged by size:
2639 1170 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SanDI GG'GwC_CC
Cuts at: 302 302
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 1070 3686 1070
Size: 2616 1553
SfcI C'TryA_G
Cuts at: 33 381 684 837 984 1212 1897 2890 3081
Size: 348 303 153 147 228 685 993 191
Cuts at: 3081 33
Size: 1121
Fragments arranged by size:
1121 993 685 348 303 228 191 153
147
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1120 1324 2239 2731 2993 1120
Size: 204 915 492 262 2296
Fragments arranged by size:
2296 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 508 1222 1722 508
Size: 714 500 2955
Fragments arranged by size:
2955 714 500
SspI AAT'ATT
Cuts at: 287 287
Size: 4169
StyI C'CwwG_G
Cuts at: 107 228 650 1303 1657 107
Size: 121 422 653 354 2619
Fragments arranged by size:
2619 653 422 354 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 503 805 1752 1885 2344 2553 503
Size: 302 947 133 459 209 2119
Fragments arranged by size:
2119 947 459 302 209 133
TatI w'GTAC_w
Cuts at: 1068 3684 1068
Size: 2616 1553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 478 503 510 535 3686 3718 224
Size: 32 222 25 7 25 3151 32 675
Fragments arranged by size:
3151 675 222 32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 810 948 1664 741
Size: 69 138 716 3246
Fragments arranged by size:
3246 716 138 69
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BpmI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI