(Circular) (Six-base) MAPSORT of: pF1KE0580.seq Check: 9048 from: 1 to: 3581 pF1KE0580 3581 bp With 182 enzymes: SgfI * April 24, 2009 13:16 .. AccI GT'mk_AC Cuts at: 619 619 Size: 3581 Acc65I G'GTAC_C Cuts at: 597 597 Size: 3581 AclI AA'CG_TT Cuts at: 3421 3421 Size: 3581 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 167 1406 1838 2585 2853 167 Size: 1239 432 747 268 895 Fragments arranged by size: 1239 895 747 432 268 AflIII A'CryG_T Cuts at: 933 2037 3014 933 Size: 1104 977 1500 Fragments arranged by size: 1500 1104 977 AgeI A'CCGG_T Cuts at: 1969 1969 Size: 3581 AlwNI CAG_nnn'CTG Cuts at: 886 2453 886 Size: 1567 2014 ApaLI G'TGCA_C Cuts at: 2351 2351 Size: 3581 ApoI r'AATT_y Cuts at: 585 2934 3190 585 Size: 2349 256 976 Fragments arranged by size: 2349 976 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3581 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3581 AvaI C'yCGr_G Cuts at: 601 601 Size: 3581 BamHI G'GATC_C Cuts at: 606 606 Size: 3581 BanI G'GyrC_C Cuts at: 597 1011 1259 1294 597 Size: 414 248 35 2884 Fragments arranged by size: 2884 414 248 35 BanII G_rGCy'C Cuts at: 595 1625 595 Size: 1030 2551 BbeI G_GCGC'C Cuts at: 1015 1263 1015 Size: 248 3333 BbsI GAAGACnn'nnnn_ Cuts at: 229 229 Size: 3581 BciVI GTATCCnnnnn_n' Cuts at: 1479 2240 3581 1479 Size: 761 1341 1479 Fragments arranged by size: 1479 1341 761 BclI T'GATC_A Cuts at: 1101 1101 Size: 3581 BglI GCCn_nnn'nGGC Cuts at: 184 184 Size: 3581 BglII A'GATC_T Cuts at: 1096 3133 1096 Size: 2037 1544 BlpI GC'TnA_GC Cuts at: 693 693 Size: 3581 Bme1580I G_kGCm'C Cuts at: 1206 1299 2355 1206 Size: 93 1056 2432 Fragments arranged by size: 2432 1056 93 BmgBI CAC'GTC Cuts at: 233 233 Size: 3581 BmrI ACTGGGnnnn_n' Cuts at: 793 874 1209 3353 793 Size: 81 335 2144 1021 Fragments arranged by size: 2144 1021 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1948 1948 Size: 3581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 757 1636 2128 2426 757 Size: 879 492 298 1912 Fragments arranged by size: 1912 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3285 3285 Size: 3581 BsaAI yAC'GTr Cuts at: 934 934 Size: 3581 BsaBI GATnn'nnATC Cuts at: 6 1095 3132 6 Size: 1089 2037 455 Fragments arranged by size: 2037 1089 455 BsaHI Gr'CG_yC Cuts at: 267 1012 1260 1962 3458 267 Size: 745 248 702 1496 390 Fragments arranged by size: 1496 745 702 390 248 BsaWI w'CCGG_w Cuts at: 770 1291 1969 2243 2390 2801 770 Size: 521 678 274 147 411 1550 Fragments arranged by size: 1550 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1006 1764 2341 2837 1006 Size: 758 577 496 1750 Fragments arranged by size: 1750 758 577 496 BsiEI CG_ry'CG Cuts at: 104 365 1123 1169 2377 104 Size: 261 758 46 1208 1308 Fragments arranged by size: 1308 1208 758 261 46 BsiHKAI G_wGCw'C Cuts at: 301 595 1373 1563 2355 301 Size: 294 778 190 792 1527 Fragments arranged by size: 1527 792 778 294 190 Bsp1286I G_dGCh'C Cuts at: 301 595 1206 1299 1373 1563 1625 2355 301 Size: 294 611 93 74 190 62 730 1527 Fragments arranged by size: 1527 730 611 294 190 93 74 62 BspEI T'CCGG_A Cuts at: 2801 2801 Size: 3581 BspHI T'CATG_A Cuts at: 2757 2757 Size: 3581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 631 845 1147 1528 631 Size: 214 302 381 2684 Fragments arranged by size: 2684 381 302 214 BsrBI CCG'CTC Cuts at: 50 1873 1927 3411 3576 50 Size: 1823 54 1484 165 55 Fragments arranged by size: 1823 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1493 1493 Size: 3581 BsrFI r'CCGG_y Cuts at: 1579 1760 1969 1579 Size: 181 209 3191 Fragments arranged by size: 3191 209 181 BssSI C'ACGA_G Cuts at: 1852 2210 1852 Size: 358 3223 BstAPI GCAn_nnn'nTGC Cuts at: 958 958 Size: 3581 BstBI TT'CG_AA Cuts at: 1942 1942 Size: 3581 BstEII G'GTnAC_C Cuts at: 477 477 Size: 3581 BstYI r'GATC_y Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787 Size: 605 467 23 335 246 1012 86 12 Cuts at: 2787 3133 1 Size: 346 449 Fragments arranged by size: 1012 605 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 353 107 Size: 246 3335 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1531 1712 1875 1531 Size: 181 163 3237 Fragments arranged by size: 3237 181 163 BtsI GCAGTG_nn' Cuts at: 259 824 259 Size: 565 3016 DraI TTT'AAA Cuts at: 173 580 173 Size: 407 3174 DrdI GACnn_nn'nnGTC Cuts at: 231 357 1287 2145 231 Size: 126 930 858 1667 Fragments arranged by size: 1667 930 858 126 EaeI y'GGCC_r Cuts at: 321 362 470 1166 1340 1731 1758 3103 321 Size: 41 108 696 174 391 27 1345 799 Fragments arranged by size: 1345 799 696 391 174 108 41 27 EagI C'GGCC_G Cuts at: 362 1166 362 Size: 804 2777 EarI CTCTTCn'nnn_ Cuts at: 289 364 1604 1814 289 Size: 75 1240 210 2056 Fragments arranged by size: 2056 1240 210 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 482 1789 2099 2245 3391 482 Size: 1307 310 146 1146 672 Fragments arranged by size: 1307 1146 672 310 146 EcoICRI GAG'CTC Cuts at: 593 593 Size: 3581 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 167 1406 1838 2585 2853 167 Size: 1239 432 747 268 895 Fragments arranged by size: 1239 895 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 720 720 Size: 3581 EcoRI G'AATT_C Cuts at: 585 585 Size: 3581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1600 1600 Size: 3581 FspI TGC'GCA Cuts at: 1362 1362 Size: 3581 HaeII r_GCGC'y Cuts at: 115 1015 1263 2285 3264 115 Size: 900 248 1022 979 432 Fragments arranged by size: 1022 979 900 432 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1423 1453 1455 1485 1423 Size: 30 2 30 3519 Fragments arranged by size: 3519 30 30 2 HincII GTy'rAC Cuts at: 620 620 Size: 3581 HindIII A'AGCT_T Cuts at: 316 316 Size: 3581 KasI G'GCGC_C Cuts at: 1011 1259 1011 Size: 248 3333 KpnI G_GTAC'C Cuts at: 601 601 Size: 3581 MluI A'CGCG_T Cuts at: 3014 3014 Size: 3581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 527 2252 2436 527 Size: 1725 184 1672 Fragments arranged by size: 1725 1672 184 MscI TGG'CCA Cuts at: 323 1342 323 Size: 1019 2562 MslI CAynn'nnrTG Cuts at: 1570 1697 1570 Size: 127 3454 MspA1I CmG'CkG Cuts at: 249 348 692 1006 1366 2379 2624 249 Size: 99 344 314 360 1013 245 1206 Fragments arranged by size: 1206 1013 360 344 314 245 99 NaeI GCC'GGC Cuts at: 1762 1762 Size: 3581 NarI GG'CG_CC Cuts at: 1012 1260 1012 Size: 248 3333 NcoI C'CATG_G Cuts at: 107 107 Size: 3581 NgoMIV G'CCGG_C Cuts at: 1760 1760 Size: 3581 NspI r_CATG'y Cuts at: 518 634 1134 2041 518 Size: 116 500 907 2058 Fragments arranged by size: 2058 907 500 116 PciI A'CATG_T Cuts at: 2037 2037 Size: 3581 PmeI GTTT'AAAC Cuts at: 580 580 Size: 3581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2720 2752 2720 Size: 32 3549 PstI C_TGCA'G Cuts at: 628 1313 628 Size: 685 2896 PvuI CG_AT'CG Cuts at: 104 104 Size: 3581 PvuII CAG'CTG Cuts at: 1006 1366 1006 Size: 360 3221 RsrII CG'GwC_CG Cuts at: 1776 1776 Size: 3581 SacI G_AGCT'C Cuts at: 595 595 Size: 3581 SalI G'TCGA_C Cuts at: 618 618 Size: 3581 SapI GCTCTTCn'nnn_ Cuts at: 289 1604 1814 289 Size: 1315 210 2056 Fragments arranged by size: 2056 1315 210 SbfI CC_TGCA'GG Cuts at: 628 628 Size: 3581 ScaI AGT'ACT Cuts at: 3098 3098 Size: 3581 SfcI C'TryA_G Cuts at: 33 624 1309 2302 2493 33 Size: 591 685 993 191 1121 Fragments arranged by size: 1121 993 685 591 191 SfoI GGC'GCC Cuts at: 1013 1261 1013 Size: 248 3333 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3581 SmaI CCC'GGG Cuts at: 603 603 Size: 3581 SmlI C'TyrA_G Cuts at: 736 1651 2143 2405 736 Size: 915 492 262 1912 Fragments arranged by size: 1912 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3581 SphI G_CATG'C Cuts at: 634 1134 634 Size: 500 3081 StyI C'CwwG_G Cuts at: 107 289 715 1069 107 Size: 182 426 354 2619 Fragments arranged by size: 2619 426 354 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1164 1297 1756 1965 1164 Size: 133 459 209 2780 Fragments arranged by size: 2780 459 209 133 TatI w'GTAC_w Cuts at: 3096 3096 Size: 3581 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 227 259 474 506 3098 3130 227 Size: 32 215 32 2592 32 678 Fragments arranged by size: 2592 678 215 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1378 1378 Size: 3581 XbaI T'CTAG_A Cuts at: 612 612 Size: 3581 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1076 1076 Size: 3581 XmaI C'CCGG_G Cuts at: 601 601 Size: 3581 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI