Restriction map for
pF1KE0583
(Circular) (Six-base) MAPSORT of: pF1KE0583.seq Check: 1695 from: 1 to: 3929
pF1KE0583 3929 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 805 805
Size: 3929
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1754 2186 2933 3201 422
Size: 1332 432 747 268 1150
Fragments arranged by size:
1332 1150 747 432 268
AflIII A'CryG_T
Cuts at: 1281 2385 3362 1281
Size: 1104 977 1848
Fragments arranged by size:
1848 1104 977
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 1234 2801 1234
Size: 1567 2362
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 521 933 3282 3538 521
Size: 412 2349 256 912
Fragments arranged by size:
2349 912 412 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 153 945 1359 1607 1642 153
Size: 792 414 248 35 2440
Fragments arranged by size:
2440 792 414 248 35
BanII G_rGCy'C
Cuts at: 176 582 884 943 1973 176
Size: 406 302 59 1030 2132
Fragments arranged by size:
2132 1030 406 302 59
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BbsI GAAGACnn'nnnn_
Cuts at: 634 634
Size: 3929
BciVI GTATCCnnnnn_n'
Cuts at: 565 1827 2588 3929 565
Size: 1262 761 1341 565
Fragments arranged by size:
1341 1262 761 565
BclI T'GATC_A
Cuts at: 857 1449 857
Size: 592 3337
BglI GCCn_nnn'nGGC
Cuts at: 719 719
Size: 3929
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 1554 1647 2703 1554
Size: 93 1056 2780
Fragments arranged by size:
2780 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 667 3633 667
Size: 2966 963
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 802 1360 1608 2310 3806 802
Size: 558 248 702 1496 925
Fragments arranged by size:
1496 925 702 558 248
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 859
Size: 3929
BseYI C'CCAG_C
Cuts at: 641 647 1354 2112 2689 3185 641
Size: 6 707 758 577 496 1385
Fragments arranged by size:
1385 758 707 577 496 6
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 943 1721 1911 2703 176
Size: 767 778 190 792 1402
Fragments arranged by size:
1402 792 778 767 190
BsmI GAATG_Cn'
Cuts at: 262 262
Size: 3929
BsmBI CGTCTCn'nnnn_
Cuts at: 793 793
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 176 582 884 943 1554 1647 1721 1911 1973
Size: 406 302 59 611 93 74 190 62
Cuts at: 1973 2703 176
Size: 730 1402
Fragments arranged by size:
1402 730 611 406 302 190 93 74
62 59
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 979 1193 1495 1876 128
Size: 851 214 302 381 2181
Fragments arranged by size:
2181 851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 838 1927 2108 2317 838
Size: 1089 181 209 2450
Fragments arranged by size:
2450 1089 209 181
BsrGI T'GTAC_A
Cuts at: 307 307
Size: 3929
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 1306 1306
Size: 3929
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstYI r'GATC_y
Cuts at: 1 197 954 1421 1444 1779 2025 3037 3123
Size: 196 757 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 757 467 449 346 335 246 196
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 704 704
Size: 3929
BtgI C'CryG_G
Cuts at: 107 221 496 621 794 107
Size: 114 275 125 173 3242
Fragments arranged by size:
3242 275 173 125 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 477 839 1172 477
Size: 362 333 3234
Fragments arranged by size:
3234 362 333
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 836 1514 1688 2079 2106 3451 836
Size: 678 174 391 27 1345 1314
Fragments arranged by size:
1345 1314 678 391 174 27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 689 901 1952 2162 689
Size: 212 1051 210 2456
Fragments arranged by size:
2456 1051 212 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 941 174
Size: 767 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1754 2186 2933 3201 422
Size: 1332 432 747 268 1150
Fragments arranged by size:
1332 1150 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 592 1068 592
Size: 476 3453
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 484 1948 484
Size: 1464 2465
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 1363 1611 2633 3612 1363
Size: 248 1022 979 1680
Fragments arranged by size:
1680 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 510 542 1771 1801 1803 1833 510
Size: 32 1229 30 2 30 2606
Fragments arranged by size:
2606 1229 32 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 151 430 1918 2045 151
Size: 279 1488 127 2035
Fragments arranged by size:
2035 1488 279 127
MspA1I CmG'CkG
Cuts at: 894 1040 1354 1714 2727 2972 894
Size: 146 314 360 1013 245 1851
Fragments arranged by size:
1851 1013 360 314 245 146
NaeI GCC'GGC
Cuts at: 840 2110 840
Size: 1270 2659
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NcoI C'CATG_G
Cuts at: 107 221 496 794 107
Size: 114 275 298 3242
Fragments arranged by size:
3242 298 275 114
NdeI CA'TA_TG
Cuts at: 655 655
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 838 2108 838
Size: 1270 2659
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PpuMI rG'GwC_Cy
Cuts at: 592 592
Size: 3929
PstI C_TGCA'G
Cuts at: 484 976 1661 484
Size: 492 685 2752
Fragments arranged by size:
2752 685 492
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 176 943 176
Size: 767 3162
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SexAI A'CCwGG_T
Cuts at: 824 824
Size: 3929
SfcI C'TryA_G
Cuts at: 33 480 972 1657 2650 2841 33
Size: 447 492 685 993 191 1121
Fragments arranged by size:
1121 993 685 492 447 191
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 221 496 794 1063 1417 107
Size: 114 275 298 269 354 2619
Fragments arranged by size:
2619 354 298 275 269 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 307 3444 307
Size: 3137 792
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
ZraI GAC'GTC
Cuts at: 803 803
Size: 3929
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI