Restriction map for
pF1KE0586
(Circular) (Six-base) MAPSORT of: pF1KE0586.seq Check: 6305 from: 1 to: 3608
pF1KE0586 3608 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 646 646
Size: 3608
Acc65I G'GTAC_C
Cuts at: 624 624
Size: 3608
AclI AA'CG_TT
Cuts at: 3448 3448
Size: 3608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 1865 2612 2880 1433
Size: 432 747 268 2161
Fragments arranged by size:
2161 747 432 268
AflIII A'CryG_T
Cuts at: 960 2064 3041 960
Size: 1104 977 1527
Fragments arranged by size:
1527 1104 977
AgeI A'CCGG_T
Cuts at: 1996 1996
Size: 3608
AlwNI CAG_nnn'CTG
Cuts at: 212 455 485 542 913 2480 212
Size: 243 30 57 371 1567 1340
Fragments arranged by size:
1567 1340 371 243 57 30
ApaLI G'TGCA_C
Cuts at: 2378 2378
Size: 3608
ApoI r'AATT_y
Cuts at: 612 2961 3217 612
Size: 2349 256 1003
Fragments arranged by size:
2349 1003 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
AvaI C'yCGr_G
Cuts at: 628 628
Size: 3608
BamHI G'GATC_C
Cuts at: 633 633
Size: 3608
BanI G'GyrC_C
Cuts at: 624 1038 1286 1321 624
Size: 414 248 35 2911
Fragments arranged by size:
2911 414 248 35
BanII G_rGCy'C
Cuts at: 295 325 622 1652 295
Size: 30 297 1030 2251
Fragments arranged by size:
2251 1030 297 30
BbeI G_GCGC'C
Cuts at: 1042 1290 1042
Size: 248 3360
BciVI GTATCCnnnnn_n'
Cuts at: 1506 2267 3608 1506
Size: 761 1341 1506
Fragments arranged by size:
1506 1341 761
BclI T'GATC_A
Cuts at: 1128 1128
Size: 3608
BglII A'GATC_T
Cuts at: 1123 3160 1123
Size: 2037 1571
BlpI GC'TnA_GC
Cuts at: 720 720
Size: 3608
Bme1580I G_kGCm'C
Cuts at: 221 1233 1326 2382 221
Size: 1012 93 1056 1447
Fragments arranged by size:
1447 1056 1012 93
BmrI ACTGGGnnnn_n'
Cuts at: 381 555 820 901 1236 3380 381
Size: 174 265 81 335 2144 609
Fragments arranged by size:
2144 609 335 265 174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 255 189
Size: 66 3542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1663 2155 2453 784
Size: 879 492 298 1939
Fragments arranged by size:
1939 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3312 3312
Size: 3608
BsaAI yAC'GTr
Cuts at: 961 961
Size: 3608
BsaBI GATnn'nnATC
Cuts at: 6 1122 3159 6
Size: 1116 2037 455
Fragments arranged by size:
2037 1116 455
BsaHI Gr'CG_yC
Cuts at: 1039 1287 1989 3485 1039
Size: 248 702 1496 1162
Fragments arranged by size:
1496 1162 702 248
BsaWI w'CCGG_w
Cuts at: 797 1318 1996 2270 2417 2828 797
Size: 521 678 274 147 411 1577
Fragments arranged by size:
1577 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 408
Size: 3608
BseYI C'CCAG_C
Cuts at: 1033 1791 2368 2864 1033
Size: 758 577 496 1777
Fragments arranged by size:
1777 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 411
Size: 3608
BsiEI CG_ry'CG
Cuts at: 104 1150 1196 2404 104
Size: 1046 46 1208 1308
Fragments arranged by size:
1308 1208 1046 46
BsiHKAI G_wGCw'C
Cuts at: 622 1400 1590 2382 622
Size: 778 190 792 1848
Fragments arranged by size:
1848 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 221 295 325 622 1233 1326 1400 1590 1652
Size: 74 30 297 611 93 74 190 62
Cuts at: 1652 2382 221
Size: 730 1447
Fragments arranged by size:
1447 730 611 297 190 93 74 74
62 30
BspEI T'CCGG_A
Cuts at: 2828 2828
Size: 3608
BspHI T'CATG_A
Cuts at: 2784 2784
Size: 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 658 872 1174 1555 658
Size: 214 302 381 2711
Fragments arranged by size:
2711 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1900 1954 3438 3603 50
Size: 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1520 1520
Size: 3608
BsrFI r'CCGG_y
Cuts at: 1606 1787 1996 1606
Size: 181 209 3218
Fragments arranged by size:
3218 209 181
BssSI C'ACGA_G
Cuts at: 1879 2237 1879
Size: 358 3250
BstAPI GCAn_nnn'nTGC
Cuts at: 551 985 551
Size: 434 3174
BstBI TT'CG_AA
Cuts at: 1969 1969
Size: 3608
BstYI r'GATC_y
Cuts at: 1 633 1100 1123 1458 1704 2716 2802 2814
Size: 632 467 23 335 246 1012 86 12
Cuts at: 2814 3160 1
Size: 346 449
Fragments arranged by size:
1012 632 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 420 507 420
Size: 87 3521
BtgI C'CryG_G
Cuts at: 107 107
Size: 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1558 1739 1902 1558
Size: 181 163 3264
Fragments arranged by size:
3264 181 163
BtsI GCAGTG_nn'
Cuts at: 851 851
Size: 3608
DraI TTT'AAA
Cuts at: 607 607
Size: 3608
DrdI GACnn_nn'nnGTC
Cuts at: 1314 2172 1314
Size: 858 2750
EaeI y'GGCC_r
Cuts at: 1193 1367 1758 1785 3130 1193
Size: 174 391 27 1345 1671
Fragments arranged by size:
1671 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1193 1193
Size: 3608
EarI CTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1816 2126 2272 3418 1816
Size: 310 146 1146 2006
Fragments arranged by size:
2006 1146 310 146
EcoICRI GAG'CTC
Cuts at: 620 620
Size: 3608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 255 1433 1865 2612 2880 189
Size: 66 1178 432 747 268 917
Fragments arranged by size:
1178 917 747 432 268 66
EcoO109I rG'GnC_Cy
Cuts at: 747 747
Size: 3608
EcoRI G'AATT_C
Cuts at: 612 612
Size: 3608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1627 1627
Size: 3608
FspI TGC'GCA
Cuts at: 1389 1389
Size: 3608
HaeII r_GCGC'y
Cuts at: 1042 1290 2312 3291 1042
Size: 248 1022 979 1359
Fragments arranged by size:
1359 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1480 1482 1512 1450
Size: 30 2 30 3546
Fragments arranged by size:
3546 30 30 2
HincII GTy'rAC
Cuts at: 647 647
Size: 3608
KasI G'GCGC_C
Cuts at: 1038 1286 1038
Size: 248 3360
KpnI G_GTAC'C
Cuts at: 628 628
Size: 3608
MluI A'CGCG_T
Cuts at: 3041 3041
Size: 3608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2463 2279
Size: 184 3424
MscI TGG'CCA
Cuts at: 1369 1369
Size: 3608
MslI CAynn'nnrTG
Cuts at: 1597 1724 1597
Size: 127 3481
MspA1I CmG'CkG
Cuts at: 209 275 395 452 482 719 1033 1393 2406
Size: 66 120 57 30 237 314 360 1013
Cuts at: 2406 2651 209
Size: 245 1166
Fragments arranged by size:
1166 1013 360 314 245 237 120 66
57 30
NaeI GCC'GGC
Cuts at: 1789 1789
Size: 3608
NarI GG'CG_CC
Cuts at: 1039 1287 1039
Size: 248 3360
NcoI C'CATG_G
Cuts at: 107 107
Size: 3608
NgoMIV G'CCGG_C
Cuts at: 1787 1787
Size: 3608
NspI r_CATG'y
Cuts at: 661 1161 2068 661
Size: 500 907 2201
Fragments arranged by size:
2201 907 500
PciI A'CATG_T
Cuts at: 2064 2064
Size: 3608
PmeI GTTT'AAAC
Cuts at: 607 607
Size: 3608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 3576
PstI C_TGCA'G
Cuts at: 655 1340 655
Size: 685 2923
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3608
PvuII CAG'CTG
Cuts at: 209 275 395 452 482 1033 1393 209
Size: 66 120 57 30 551 360 2424
Fragments arranged by size:
2424 551 360 120 66 57 30
RsrII CG'GwC_CG
Cuts at: 1803 1803
Size: 3608
SacI G_AGCT'C
Cuts at: 622 622
Size: 3608
SalI G'TCGA_C
Cuts at: 645 645
Size: 3608
SapI GCTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
SbfI CC_TGCA'GG
Cuts at: 655 655
Size: 3608
ScaI AGT'ACT
Cuts at: 3125 3125
Size: 3608
SfcI C'TryA_G
Cuts at: 33 651 1336 2329 2520 33
Size: 618 685 993 191 1121
Fragments arranged by size:
1121 993 685 618 191
SfoI GGC'GCC
Cuts at: 1040 1288 1040
Size: 248 3360
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
SmaI CCC'GGG
Cuts at: 630 630
Size: 3608
SmlI C'TyrA_G
Cuts at: 763 1678 2170 2432 763
Size: 915 492 262 1939
Fragments arranged by size:
1939 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3608
SphI G_CATG'C
Cuts at: 661 1161 661
Size: 500 3108
StyI C'CwwG_G
Cuts at: 107 296 326 742 1096 107
Size: 189 30 416 354 2619
Fragments arranged by size:
2619 416 354 189 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1191 1324 1783 1992 1191
Size: 133 459 209 2807
Fragments arranged by size:
2807 459 209 133
TatI w'GTAC_w
Cuts at: 3123 3123
Size: 3608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3576
Tth111I GACn'n_nGTC
Cuts at: 1405 1405
Size: 3608
XbaI T'CTAG_A
Cuts at: 639 639
Size: 3608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1103 1103
Size: 3608
XmaI C'CCGG_G
Cuts at: 628 628
Size: 3608
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI