Restriction map for
pF1KE0587
(Circular) (Six-base) MAPSORT of: pF1KE0587.seq Check: 1462 from: 1 to: 3947
pF1KE0587 3947 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 985 985
Size: 3947
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1772 2204 2951 3219 1772
Size: 432 747 268 2500
Fragments arranged by size:
2500 747 432 268
AflIII A'CryG_T
Cuts at: 1299 2403 3380 1299
Size: 1104 977 1866
Fragments arranged by size:
1866 1104 977
AgeI A'CCGG_T
Cuts at: 2335 2335
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 233 497 824 881 911 1252 2819 233
Size: 264 327 57 30 341 1567 1361
Fragments arranged by size:
1567 1361 341 327 264 57 30
ApaLI G'TGCA_C
Cuts at: 2717 2717
Size: 3947
ApoI r'AATT_y
Cuts at: 951 3300 3556 951
Size: 2349 256 1342
Fragments arranged by size:
2349 1342 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3947
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 967 967
Size: 3947
BamHI G'GATC_C
Cuts at: 388 448 682 972 388
Size: 60 234 290 3363
Fragments arranged by size:
3363 290 234 60
BanI G'GyrC_C
Cuts at: 963 1377 1625 1660 963
Size: 414 248 35 3250
Fragments arranged by size:
3250 414 248 35
BanII G_rGCy'C
Cuts at: 331 421 625 961 1991 331
Size: 90 204 336 1030 2287
Fragments arranged by size:
2287 1030 336 204 90
BbeI G_GCGC'C
Cuts at: 1381 1629 1381
Size: 248 3699
BciVI GTATCCnnnnn_n'
Cuts at: 1845 2606 3947 1845
Size: 761 1341 1845
Fragments arranged by size:
1845 1341 761
BclI T'GATC_A
Cuts at: 1467 1467
Size: 3947
BglII A'GATC_T
Cuts at: 1462 3499 1462
Size: 2037 1910
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 242 740 1572 1665 2721 242
Size: 498 832 93 1056 1468
Fragments arranged by size:
1468 1056 832 498 93
BmrI ACTGGGnnnn_n'
Cuts at: 807 894 1159 1240 1575 3719 807
Size: 87 265 81 335 2144 1035
Fragments arranged by size:
2144 1035 335 265 87 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 210 276 474 540 126
Size: 84 66 198 66 3533
Fragments arranged by size:
3533 198 84 66 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2002 2494 2792 1123
Size: 879 492 298 2278
Fragments arranged by size:
2278 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3651 3651
Size: 3947
BsaAI yAC'GTr
Cuts at: 1300 1300
Size: 3947
BsaBI GATnn'nnATC
Cuts at: 6 1461 3498 6
Size: 1455 2037 455
Fragments arranged by size:
2037 1455 455
BsaHI Gr'CG_yC
Cuts at: 1378 1626 2328 3824 1378
Size: 248 702 1496 1501
Fragments arranged by size:
1501 1496 702 248
BsaWI w'CCGG_w
Cuts at: 127 1136 1657 2335 2609 2756 3167 127
Size: 1009 521 678 274 147 411 907
Fragments arranged by size:
1009 907 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1372 2130 2707 3203 1372
Size: 758 577 496 2116
Fragments arranged by size:
2116 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 837
Size: 3947
BsiEI CG_ry'CG
Cuts at: 104 1489 1535 2743 104
Size: 1385 46 1208 1308
Fragments arranged by size:
1385 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 595 961 1739 1929 2721 595
Size: 366 778 190 792 1821
Fragments arranged by size:
1821 792 778 366 190
Bsp1286I G_dGCh'C
Cuts at: 242 331 421 595 625 740 961 1572 1665
Size: 89 90 174 30 115 221 611 93
Cuts at: 1665 1739 1929 1991 2721 242
Size: 74 190 62 730 1468
Fragments arranged by size:
1468 730 611 221 190 174 115 93
90 89 74 62 30
BspEI T'CCGG_A
Cuts at: 127 3167 127
Size: 3040 907
BspHI T'CATG_A
Cuts at: 3123 3123
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 997 1211 1513 1894 997
Size: 214 302 381 3050
Fragments arranged by size:
3050 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 1859
Size: 3947
BsrFI r'CCGG_y
Cuts at: 1945 2126 2335 1945
Size: 181 209 3557
Fragments arranged by size:
3557 209 181
BssSI C'ACGA_G
Cuts at: 2218 2576 2218
Size: 358 3589
BstAPI GCAn_nnn'nTGC
Cuts at: 890 1324 890
Size: 434 3513
BstBI TT'CG_AA
Cuts at: 2308 2308
Size: 3947
BstYI r'GATC_y
Cuts at: 1 388 448 682 972 1439 1462 1797 2043
Size: 387 60 234 290 467 23 335 246
Cuts at: 2043 3055 3141 3153 3499 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 387 346 335 290 246
234 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 846 846
Size: 3947
BtgI C'CryG_G
Cuts at: 107 107
Size: 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 1190 1190
Size: 3947
DraI TTT'AAA
Cuts at: 946 946
Size: 3947
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2511 1653
Size: 858 3089
EaeI y'GGCC_r
Cuts at: 1532 1706 2097 2124 3469 1532
Size: 174 391 27 1345 2010
Fragments arranged by size:
2010 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1532 1532
Size: 3947
EarI CTCTTCn'nnn_
Cuts at: 324 1970 2180 324
Size: 1646 210 2091
Fragments arranged by size:
2091 1646 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2465 2611 3757 2155
Size: 310 146 1146 2345
Fragments arranged by size:
2345 1146 310 146
EcoICRI GAG'CTC
Cuts at: 959 959
Size: 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 210 276 474 540 1772 2204 2951 3219
Size: 84 66 198 66 1232 432 747 268
Cuts at: 3219 126
Size: 854
Fragments arranged by size:
1232 854 747 432 268 198 84 66
66
EcoO109I rG'GnC_Cy
Cuts at: 602 1086 602
Size: 484 3463
EcoRI G'AATT_C
Cuts at: 951 951
Size: 3947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1966 1966
Size: 3947
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 1381 1629 2651 3630 1381
Size: 248 1022 979 1698
Fragments arranged by size:
1698 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1789 1819 1821 1851 1789
Size: 30 2 30 3885
Fragments arranged by size:
3885 30 30 2
HincII GTy'rAC
Cuts at: 986 986
Size: 3947
KasI G'GCGC_C
Cuts at: 1377 1625 1377
Size: 248 3699
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2618 2802 2618
Size: 184 3763
MscI TGG'CCA
Cuts at: 1708 1708
Size: 3947
MslI CAynn'nnrTG
Cuts at: 1936 2063 1936
Size: 127 3820
MspA1I CmG'CkG
Cuts at: 146 230 296 494 560 878 1058 1372 1732
Size: 84 66 198 66 318 180 314 360
Cuts at: 1732 2745 2990 146
Size: 1013 245 1103
Fragments arranged by size:
1103 1013 360 318 314 245 198 180
84 66 66
NaeI GCC'GGC
Cuts at: 2128 2128
Size: 3947
NarI GG'CG_CC
Cuts at: 1378 1626 1378
Size: 248 3699
NcoI C'CATG_G
Cuts at: 107 107
Size: 3947
NgoMIV G'CCGG_C
Cuts at: 2126 2126
Size: 3947
NspI r_CATG'y
Cuts at: 1000 1500 2407 1000
Size: 500 907 2540
Fragments arranged by size:
2540 907 500
PciI A'CATG_T
Cuts at: 2403 2403
Size: 3947
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3915
PstI C_TGCA'G
Cuts at: 994 1679 994
Size: 685 3262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 146 230 296 494 560 878 1372 1732 146
Size: 84 66 198 66 318 494 360 2361
Fragments arranged by size:
2361 494 360 318 198 84 66 66
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 961 961
Size: 3947
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SapI GCTCTTCn'nnn_
Cuts at: 324 1970 2180 324
Size: 1646 210 2091
Fragments arranged by size:
2091 1646 210
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SfcI C'TryA_G
Cuts at: 33 990 1675 2668 2859 33
Size: 957 685 993 191 1121
Fragments arranged by size:
1121 993 957 685 191
SfoI GGC'GCC
Cuts at: 1379 1627 1379
Size: 248 3699
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SmaI CCC'GGG
Cuts at: 969 969
Size: 3947
SmlI C'TyrA_G
Cuts at: 1102 2017 2509 2771 1102
Size: 915 492 262 2278
Fragments arranged by size:
2278 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
StyI C'CwwG_G
Cuts at: 107 332 392 422 452 596 626 686 1081
Size: 225 60 30 30 144 30 60 395
Cuts at: 1081 1435 107
Size: 354 2619
Fragments arranged by size:
2619 395 354 225 144 60 60 30
30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 578 1530 1663 2122 2331 578
Size: 952 133 459 209 2194
Fragments arranged by size:
2194 952 459 209 133
TatI w'GTAC_w
Cuts at: 3462 3462
Size: 3947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 1744 1744
Size: 3947
XbaI T'CTAG_A
Cuts at: 978 978
Size: 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1442 1442
Size: 3947
XmaI C'CCGG_G
Cuts at: 967 967
Size: 3947
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI