Restriction map for
pF1KE0592
(Circular) (Six-base) MAPSORT of: pF1KE0592.seq Check: 6676 from: 1 to: 4541
pF1KE0592 4541 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1196 1579 1196
Size: 383 4158
Acc65I G'GTAC_C
Cuts at: 1557 1557
Size: 4541
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1026 1309 2366 2798 3545 3813 257
Size: 769 283 1057 432 747 268 985
Fragments arranged by size:
1057 985 769 747 432 283 268
AflIII A'CryG_T
Cuts at: 1085 1893 2997 3974 1085
Size: 808 1104 977 1652
Fragments arranged by size:
1652 1104 977 808
AgeI A'CCGG_T
Cuts at: 1349 2929 1349
Size: 1580 2961
AlwNI CAG_nnn'CTG
Cuts at: 1846 3413 1846
Size: 1567 2974
ApaI G_GGCC'C
Cuts at: 1102 1398 1527 1102
Size: 296 129 4116
Fragments arranged by size:
4116 296 129
ApaLI G'TGCA_C
Cuts at: 3311 3311
Size: 4541
ApoI r'AATT_y
Cuts at: 118 161 545 730 1545 3894 4150 118
Size: 43 384 185 815 2349 256 509
Fragments arranged by size:
2349 815 509 384 256 185 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 1561 1561
Size: 4541
AvrII C'CTAG_G
Cuts at: 1419 1419
Size: 4541
BamHI G'GATC_C
Cuts at: 1445 1566 1445
Size: 121 4420
BanI G'GyrC_C
Cuts at: 394 1072 1423 1557 1971 2219 2254 394
Size: 678 351 134 414 248 35 2681
Fragments arranged by size:
2681 678 414 351 248 134 35
BanII G_rGCy'C
Cuts at: 438 453 795 1102 1219 1398 1527 1555 2585
Size: 15 342 307 117 179 129 28 1030
Cuts at: 2585 438
Size: 2394
Fragments arranged by size:
2394 1030 342 307 179 129 117 28
15
BbeI G_GCGC'C
Cuts at: 398 1427 1975 2223 398
Size: 1029 548 248 2716
Fragments arranged by size:
2716 1029 548 248
BbsI GAAGACnn'nnnn_
Cuts at: 263 1082 1181 263
Size: 819 99 3623
Fragments arranged by size:
3623 819 99
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3200 4541 2439
Size: 761 1341 2439
Fragments arranged by size:
2439 1341 761
BclI T'GATC_A
Cuts at: 470 2061 470
Size: 1591 2950
BglI GCCn_nnn'nGGC
Cuts at: 1070 1070
Size: 4541
BglII A'GATC_T
Cuts at: 1484 2056 4093 1484
Size: 572 2037 1932
Fragments arranged by size:
2037 1932 572
BlpI GC'TnA_GC
Cuts at: 1653 1653
Size: 4541
Bme1580I G_kGCm'C
Cuts at: 1075 1102 1398 1527 2166 2259 3315 1075
Size: 27 296 129 639 93 1056 2301
Fragments arranged by size:
2301 1056 639 296 129 93 27
BmgBI CAC'GTC
Cuts at: 365 1088 365
Size: 723 3818
BmrI ACTGGGnnnn_n'
Cuts at: 1753 1834 2169 4313 1753
Size: 81 335 2144 1981
Fragments arranged by size:
2144 1981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1133
Size: 4541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2596 3088 3386 1717
Size: 879 492 298 2872
Fragments arranged by size:
2872 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1257 1318 4245 1257
Size: 61 2927 1553
Fragments arranged by size:
2927 1553 61
BsaAI yAC'GTr
Cuts at: 1108 1894 1108
Size: 786 3755
BsaBI GATnn'nnATC
Cuts at: 6 2055 4092 6
Size: 2049 2037 455
Fragments arranged by size:
2049 2037 455
BsaHI Gr'CG_yC
Cuts at: 395 1424 1972 2220 2922 4418 395
Size: 1029 548 248 702 1496 518
Fragments arranged by size:
1496 1029 702 548 518 248
BsaWI w'CCGG_w
Cuts at: 1349 1730 2251 2929 3203 3350 3761 1349
Size: 381 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 381 274 147
BseYI C'CCAG_C
Cuts at: 1068 1101 1212 1966 2724 3301 3797 1068
Size: 33 111 754 758 577 496 1812
Fragments arranged by size:
1812 758 754 577 496 111 33
BsiEI CG_ry'CG
Cuts at: 104 2083 2129 3337 104
Size: 1979 46 1208 1308
Fragments arranged by size:
1979 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 438 1555 2333 2523 3315 438
Size: 1117 778 190 792 1664
Fragments arranged by size:
1664 1117 792 778 190
BsmI GAATG_Cn'
Cuts at: 691 1225 691
Size: 534 4007
Bsp1286I G_dGCh'C
Cuts at: 438 453 795 1075 1102 1219 1398 1527 1555
Size: 15 342 280 27 117 179 129 28
Cuts at: 1555 2166 2259 2333 2523 2585 3315 438
Size: 611 93 74 190 62 730 1664
Fragments arranged by size:
1664 730 611 342 280 190 179 129
117 93 74 62 28 27 15
BspEI T'CCGG_A
Cuts at: 3761 3761
Size: 4541
BspHI T'CATG_A
Cuts at: 833 3717 833
Size: 2884 1657
BspMI ACCTGCnnnn'nnnn_
Cuts at: 908 1591 1805 2107 2488 908
Size: 683 214 302 381 2961
Fragments arranged by size:
2961 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2833 2887 4371 4536 50
Size: 2783 54 1484 165 55
Fragments arranged by size:
2783 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 653 2453 653
Size: 1800 2741
BsrFI r'CCGG_y
Cuts at: 1349 2539 2720 2929 1349
Size: 1190 181 209 2961
Fragments arranged by size:
2961 1190 209 181
BssSI C'ACGA_G
Cuts at: 2812 3170 2812
Size: 358 4183
BstAPI GCAn_nnn'nTGC
Cuts at: 1918 1918
Size: 4541
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstYI r'GATC_y
Cuts at: 1 347 1445 1484 1566 2033 2056 2391 2637
Size: 346 1098 39 82 467 23 335 246
Cuts at: 2637 3649 3735 3747 4093 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1098 1012 467 449 346 346 335 246
86 82 39 23 12
BstZ17I GTA'TAC
Cuts at: 1197 1197
Size: 4541
BtgI C'CryG_G
Cuts at: 107 924 1331 1367 107
Size: 817 407 36 3281
Fragments arranged by size:
3281 817 407 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 732 828 2491 2672 2835 732
Size: 96 1663 181 163 2438
Fragments arranged by size:
2438 1663 181 163 96
BtsI GCAGTG_nn'
Cuts at: 872 1784 872
Size: 912 3629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 837 872 837
Size: 35 4506
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 483 717 767 927 2126 2300 2691 2718 4063
Size: 234 50 160 1199 174 391 27 1345
Cuts at: 4063 483
Size: 961
Fragments arranged by size:
1345 1199 961 391 234 174 160 50
27
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 445 655 961 1537 2564 2774 445
Size: 210 306 576 1027 210 2212
Fragments arranged by size:
2212 1027 576 306 210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2749 3059 3205 4351 2749
Size: 310 146 1146 2939
Fragments arranged by size:
2939 1146 310 146
EcoICRI GAG'CTC
Cuts at: 436 1553 436
Size: 1117 3424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1026 1133 1309 2366 2798 3545 3813 257
Size: 769 107 176 1057 432 747 268 985
Fragments arranged by size:
1057 985 769 747 432 268 176 107
EcoNI CCTnn'n_nnAGG
Cuts at: 799 799
Size: 4541
EcoO109I rG'GnC_Cy
Cuts at: 522 1098 1394 1395 1523 1524 1680 522
Size: 576 296 1 128 1 156 3383
Fragments arranged by size:
3383 576 296 156 128 1 1
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4541
FspI TGC'GCA
Cuts at: 2322 2322
Size: 4541
HaeII r_GCGC'y
Cuts at: 398 1427 1975 2223 3245 4224 398
Size: 1029 548 248 1022 979 715
Fragments arranged by size:
1029 1022 979 715 548 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2383 2413 2415 2445 2383
Size: 30 2 30 4479
Fragments arranged by size:
4479 30 30 2
HincII GTy'rAC
Cuts at: 711 1580 711
Size: 869 3672
KasI G'GCGC_C
Cuts at: 394 1423 1971 2219 394
Size: 1029 548 248 2716
Fragments arranged by size:
2716 1029 548 248
KpnI G_GTAC'C
Cuts at: 1561 1561
Size: 4541
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 1028 1061 3212 3396 992
Size: 36 33 2151 184 2137
Fragments arranged by size:
2151 2137 184 36 33
MscI TGG'CCA
Cuts at: 769 929 2302 769
Size: 160 1373 3008
Fragments arranged by size:
3008 1373 160
MslI CAynn'nnrTG
Cuts at: 334 2530 2657 334
Size: 2196 127 2218
Fragments arranged by size:
2218 2196 127
MspA1I CmG'CkG
Cuts at: 283 1287 1369 1652 1966 2326 3339 3584 283
Size: 1004 82 283 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 1004 360 314 283 245 82
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 395 1424 1972 2220 395
Size: 1029 548 248 2716
Fragments arranged by size:
2716 1029 548 248
NcoI C'CATG_G
Cuts at: 107 1331 107
Size: 1224 3317
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 1594 2094 3001 1594
Size: 500 907 3134
Fragments arranged by size:
3134 907 500
PciI A'CATG_T
Cuts at: 2997 2997
Size: 4541
PflMI CCAn_nnn'nTGG
Cuts at: 683 765 1123 1241 683
Size: 82 358 118 3983
Fragments arranged by size:
3983 358 118 82
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PmlI CAC'GTG
Cuts at: 1108 1108
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 522 522
Size: 4541
PsiI TTA'TAA
Cuts at: 376 376
Size: 4541
PspOMI G'GGCC_C
Cuts at: 1098 1394 1523 1098
Size: 296 129 4116
Fragments arranged by size:
4116 296 129
PstI C_TGCA'G
Cuts at: 989 1588 2273 989
Size: 599 685 3257
Fragments arranged by size:
3257 685 599
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 283 1287 1966 2326 283
Size: 1004 679 360 2498
Fragments arranged by size:
2498 1004 679 360
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 438 1555 438
Size: 1117 3424
SacII CC_GC'GG
Cuts at: 1370 1370
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 522 522
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 961 1537 2564 2774 961
Size: 576 1027 210 2728
Fragments arranged by size:
2728 1027 576 210
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 1477 4058 1477
Size: 2581 1960
SexAI A'CCwGG_T
Cuts at: 1075 1075
Size: 4541
SfcI C'TryA_G
Cuts at: 33 985 1584 2269 3262 3453 33
Size: 952 599 685 993 191 1121
Fragments arranged by size:
1121 993 952 685 599 191
SfoI GGC'GCC
Cuts at: 396 1425 1973 2221 396
Size: 1029 548 248 2716
Fragments arranged by size:
2716 1029 548 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SmaI CCC'GGG
Cuts at: 1563 1563
Size: 4541
SmlI C'TyrA_G
Cuts at: 1696 2611 3103 3365 1696
Size: 915 492 262 2872
Fragments arranged by size:
2872 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 1594 2094 1594
Size: 500 4041
StyI C'CwwG_G
Cuts at: 107 630 1331 1419 1675 2029 107
Size: 523 701 88 256 354 2619
Fragments arranged by size:
2619 701 523 354 256 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 446 526 1392 2124 2257 2716 2925 446
Size: 80 866 732 133 459 209 2062
Fragments arranged by size:
2062 866 732 459 209 133 80
TatI w'GTAC_w
Cuts at: 932 1475 4056 932
Size: 543 2581 1417
Fragments arranged by size:
2581 1417 543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 4509
Tth111I GACn'n_nGTC
Cuts at: 784 1180 2338 784
Size: 396 1158 2987
Fragments arranged by size:
2987 1158 396
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 460 2036 460
Size: 1576 2965
XmaI C'CCGG_G
Cuts at: 1561 1561
Size: 4541
XmnI GAAnn'nnTTC
Cuts at: 691 691
Size: 4541
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PshAI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI