Restriction map for
pF1KE0597
(Circular) (Six-base) MAPSORT of: pF1KE0597.seq Check: 756 from: 1 to: 7562
pF1KE0597 7562 bp
With 182 enzymes: SgfI *
June 11, 2012 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1284 1284
Size: 7562
AatII G_ACGT'C
Cuts at: 2332 2332
Size: 7562
AccI GT'mk_AC
Cuts at: 4600 4600
Size: 7562
Acc65I G'GTAC_C
Cuts at: 3179 4578 3179
Size: 1399 6163
AclI AA'CG_TT
Cuts at: 7402 7402
Size: 7562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1214 1411 1745 1925 2633 2741 3391 3866 4087
Size: 197 334 180 708 108 650 475 221
Cuts at: 4087 4170 4182 4514 5387 5819 6566 6834 1214
Size: 83 12 332 873 432 747 268 1942
Fragments arranged by size:
1942 873 747 708 650 475 432 334
332 268 221 197 180 108 83 12
AflIII A'CryG_T
Cuts at: 4914 6018 6995 4914
Size: 1104 977 5481
Fragments arranged by size:
5481 1104 977
AgeI A'CCGG_T
Cuts at: 5950 5950
Size: 7562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2441 2662 4396 2441
Size: 221 1734 5607
Fragments arranged by size:
5607 1734 221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2663 2695 4270 4302 2663
Size: 32 1575 32 5923
Fragments arranged by size:
5923 1575 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2611 2755 2947 4867 6434 2611
Size: 144 192 1920 1567 3739
Fragments arranged by size:
3739 1920 1567 192 144
ApaLI G'TGCA_C
Cuts at: 6332 6332
Size: 7562
ApoI r'AATT_y
Cuts at: 754 1179 1365 1473 1951 2314 3222 3343 3427
Size: 425 186 108 478 363 908 121 84
Cuts at: 3427 4566 6915 7171 754
Size: 1139 2349 256 1145
Fragments arranged by size:
2349 1145 1139 908 478 425 363 256
186 121 108 84
AseI AT'TA_AT
Cuts at: 20 677 20
Size: 657 6905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7562
AvaI C'yCGr_G
Cuts at: 209 3353 4020 4582 209
Size: 3144 667 562 3189
Fragments arranged by size:
3189 3144 667 562
AvrII C'CTAG_G
Cuts at: 1489 1489
Size: 7562
BamHI G'GATC_C
Cuts at: 1039 2372 4587 1039
Size: 1333 2215 4014
Fragments arranged by size:
4014 2215 1333
BanI G'GyrC_C
Cuts at: 111 568 3179 4016 4062 4578 4992 5240 5275
Size: 457 2611 837 46 516 414 248 35
Cuts at: 5275 111
Size: 2398
Fragments arranged by size:
2611 2398 837 516 457 414 248 46
35
BanII G_rGCy'C
Cuts at: 747 1467 1644 2230 2594 4120 4576 5606 747
Size: 720 177 586 364 1526 456 1030 2703
Fragments arranged by size:
2703 1526 1030 720 586 456 364 177
BbeI G_GCGC'C
Cuts at: 115 4020 4996 5244 115
Size: 3905 976 248 2433
Fragments arranged by size:
3905 2433 976 248
BbsI GAAGACnn'nnnn_
Cuts at: 1399 1612 2120 2621 3118 3571 4405 4551 1399
Size: 213 508 501 497 453 834 146 4410
Fragments arranged by size:
4410 834 508 501 497 453 213 146
BbvCI CC'TCA_GC
Cuts at: 4082 4352 4082
Size: 270 7292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 677 711 677
Size: 34 7528
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2006 5460 6221 7562 1890
Size: 116 3454 761 1341 1890
Fragments arranged by size:
3454 1890 1341 761 116
BclI T'GATC_A
Cuts at: 4244 5082 4244
Size: 838 6724
BfrBI ATG'CAT
Cuts at: 889 889
Size: 7562
BglI GCCn_nnn'nGGC
Cuts at: 3409 3409
Size: 7562
BglII A'GATC_T
Cuts at: 2200 2348 5077 7114 2200
Size: 148 2729 2037 2648
Fragments arranged by size:
2729 2648 2037 148
BlpI GC'TnA_GC
Cuts at: 326 4384 4674 326
Size: 4058 290 3214
Fragments arranged by size:
4058 3214 290
Bme1580I G_kGCm'C
Cuts at: 3793 5187 5280 6336 3793
Size: 1394 93 1056 5019
Fragments arranged by size:
5019 1394 1056 93
BmgBI CAC'GTC
Cuts at: 260 260
Size: 7562
BmrI ACTGGGnnnn_n'
Cuts at: 4774 4855 5190 7334 4774
Size: 81 335 2144 5002
Fragments arranged by size:
5002 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1663 5929 1663
Size: 4266 3296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 2323 3574 3579 4156 588
Size: 1735 1251 5 577 3994
Fragments arranged by size:
3994 1735 1251 577 5
Bpu10I CC'TnA_GC
Cuts at: 617 4082 4352 617
Size: 3465 270 3827
Fragments arranged by size:
3827 3465 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1840 1977 2049 4738 5617 6109 6407 481
Size: 1359 137 72 2689 879 492 298 1636
Fragments arranged by size:
2689 1636 1359 879 492 298 137 72
BsaI GGTCTCn'nnnn_
Cuts at: 2299 2401 3948 4055 7266 2299
Size: 102 1547 107 3211 2595
Fragments arranged by size:
3211 2595 1547 107 102
BsaAI yAC'GTr
Cuts at: 691 1125 3606 4915 691
Size: 434 2481 1309 3338
Fragments arranged by size:
3338 2481 1309 434
BsaBI GATnn'nnATC
Cuts at: 6 5076 7113 6
Size: 5070 2037 455
Fragments arranged by size:
5070 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 2329 4017 4993 5241 5943 7439 112
Size: 2217 1688 976 248 702 1496 235
Fragments arranged by size:
2217 1688 1496 976 702 248 235
BsaWI w'CCGG_w
Cuts at: 2369 4751 5272 5950 6224 6371 6782 2369
Size: 2382 521 678 274 147 411 3149
Fragments arranged by size:
3149 2382 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 657 687 2075 2105 382
Size: 30 245 30 1388 30 5839
Fragments arranged by size:
5839 1388 245 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 355 737 1596 1777 2350 3907 3910 3913
Size: 177 382 859 181 573 1557 3 3
Cuts at: 3913 3925 3928 3931 178
Size: 12 3 3 3809
Fragments arranged by size:
3809 1557 859 573 382 181 177 12
3 3 3 3
BseYI C'CCAG_C
Cuts at: 961 2888 3792 4987 5745 6322 6818 961
Size: 1927 904 1195 758 577 496 1705
Fragments arranged by size:
1927 1705 1195 904 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2054 3533 4294 4330 1225
Size: 829 1479 761 36 4457
Fragments arranged by size:
4457 1479 829 761 36
BsiEI CG_ry'CG
Cuts at: 104 5104 5150 6358 104
Size: 5000 46 1208 1308
Fragments arranged by size:
5000 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 690 1644 1700 4120 4576 5354 5544 6336 690
Size: 954 56 2420 456 778 190 792 1916
Fragments arranged by size:
2420 1916 954 792 778 456 190 56
BsmI GAATG_Cn'
Cuts at: 1882 2163 1882
Size: 281 7281
BsmBI CGTCTCn'nnnn_
Cuts at: 1651 1651
Size: 7562
Bsp1286I G_dGCh'C
Cuts at: 690 747 1467 1644 1700 2230 2594 3793 4120
Size: 57 720 177 56 530 364 1199 327
Cuts at: 4120 4576 5187 5280 5354 5544 5606 6336 690
Size: 456 611 93 74 190 62 730 1916
Fragments arranged by size:
1916 1199 730 720 611 530 456 364
327 190 177 93 74 62 57 56
BspEI T'CCGG_A
Cuts at: 6782 6782
Size: 7562
BspHI T'CATG_A
Cuts at: 437 2378 6738 437
Size: 1941 4360 1261
Fragments arranged by size:
4360 1941 1261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1284 4612 4826 5128 5509 1284
Size: 3328 214 302 381 3337
Fragments arranged by size:
3337 3328 381 302 214
BsrBI CCG'CTC
Cuts at: 50 117 171 4304 5854 5908 7392 7557 50
Size: 67 54 4133 1550 54 1484 165 55
Fragments arranged by size:
4133 1550 1484 165 67 55 54 54
BsrDI GCAATG_nn'
Cuts at: 1541 5474 1541
Size: 3933 3629
BsrFI r'CCGG_y
Cuts at: 3753 4013 5560 5741 5950 3753
Size: 260 1547 181 209 5365
Fragments arranged by size:
5365 1547 260 209 181
BssSI C'ACGA_G
Cuts at: 1460 5833 6191 1460
Size: 4373 358 2831
Fragments arranged by size:
4373 2831 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3541 3609 3658 4939 3541
Size: 68 49 1281 6164
Fragments arranged by size:
6164 1281 68 49
BstBI TT'CG_AA
Cuts at: 5923 5923
Size: 7562
BstXI CCAn_nnnn'nTGG
Cuts at: 1947 1947
Size: 7562
BstYI r'GATC_y
Cuts at: 1 1039 2200 2348 2372 4157 4587 5054 5077
Size: 1038 1161 148 24 1785 430 467 23
Cuts at: 5077 5412 5658 6670 6756 6768 7114 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1785 1161 1038 1012 467 449 430 346
335 246 148 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 3134 3134
Size: 7562
BtgI C'CryG_G
Cuts at: 107 1468 1940 3828 4256 4368 107
Size: 1361 472 1888 428 112 3301
Fragments arranged by size:
3301 1888 1361 472 428 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5512 5693 5856 5512
Size: 181 163 7218
Fragments arranged by size:
7218 181 163
BtsI GCAGTG_nn'
Cuts at: 2925 3793 4805 2925
Size: 868 1012 5682
Fragments arranged by size:
5682 1012 868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3724 3759 3810 3845 4350 4385 3724
Size: 35 51 35 505 35 6901
Fragments arranged by size:
6901 505 51 35 35 35
DraI TTT'AAA
Cuts at: 3263 4561 3263
Size: 1298 6264
DrdI GACnn_nn'nnGTC
Cuts at: 2941 5268 6126 2941
Size: 2327 858 4377
Fragments arranged by size:
4377 2327 858
EaeI y'GGCC_r
Cuts at: 1436 1937 4443 5147 5321 5712 5739 7084 1436
Size: 501 2506 704 174 391 27 1345 1914
Fragments arranged by size:
2506 1914 1345 704 501 391 174 27
EagI C'GGCC_G
Cuts at: 5147 5147
Size: 7562
EarI CTCTTCn'nnn_
Cuts at: 178 209 455 536 1089 1797 2473 2629 2854
Size: 31 246 81 553 708 676 156 225
Cuts at: 2854 3169 3506 3842 3884 3902 4148 5585 5795
Size: 315 337 336 42 18 246 1437 210
Cuts at: 5795 178
Size: 1945
Fragments arranged by size:
1945 1437 708 676 553 337 336 315
246 246 225 210 156 81 42 31
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5770 6080 6226 7372 5770
Size: 310 146 1146 5960
Fragments arranged by size:
5960 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1642 4118 4574 1642
Size: 2476 456 4630
Fragments arranged by size:
4630 2476 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 1214 1411 1745 1925 2323 2633 2741 3391
Size: 626 197 334 180 398 310 108 650
Cuts at: 3391 3574 3579 3866 4087 4156 4170 4182 4514
Size: 183 5 287 221 69 14 12 332
Cuts at: 4514 5387 5819 6566 6834 588
Size: 873 432 747 268 1316
Fragments arranged by size:
1316 873 747 650 626 432 398 334
332 310 287 268 221 197 183 180
108 69 14 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1265 1265
Size: 7562
EcoO109I rG'GnC_Cy
Cuts at: 1493 4701 1493
Size: 3208 4354
EcoRI G'AATT_C
Cuts at: 1179 1365 1473 1951 4566 1179
Size: 186 108 478 2615 4175
Fragments arranged by size:
4175 2615 478 186 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1834 2064 3334 4200 5581 1834
Size: 230 1270 866 1381 3815
Fragments arranged by size:
3815 1381 1270 866 230
FseI GG_CCGG'CC
Cuts at: 3757 3757
Size: 7562
FspI TGC'GCA
Cuts at: 2197 2803 5343 2197
Size: 606 2540 4416
Fragments arranged by size:
4416 2540 606
HaeII r_GCGC'y
Cuts at: 115 846 1589 4020 4996 5244 6266 7245 115
Size: 731 743 2431 976 248 1022 979 432
Fragments arranged by size:
2431 1022 979 976 743 731 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 568 576 600 2314 2346 2831 2863 5404
Size: 24 8 24 1714 32 485 32 2541
Cuts at: 5404 5434 5436 5466 544
Size: 30 2 30 2640
Fragments arranged by size:
2640 2541 1714 485 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 1862 3154 4601 1862
Size: 1292 1447 4823
Fragments arranged by size:
4823 1447 1292
HindIII A'AGCT_T
Cuts at: 364 2762 364
Size: 2398 5164
HpaI GTT'AAC
Cuts at: 1862 1862
Size: 7562
KasI G'GCGC_C
Cuts at: 111 4016 4992 5240 111
Size: 3905 976 248 2433
Fragments arranged by size:
3905 2433 976 248
KpnI G_GTAC'C
Cuts at: 3183 4582 3183
Size: 1399 6163
MluI A'CGCG_T
Cuts at: 6995 6995
Size: 7562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 1409 4506 6233 6417 410
Size: 999 3097 1727 184 1555
Fragments arranged by size:
3097 1727 1555 999 184
MscI TGG'CCA
Cuts at: 1438 1939 4445 5323 1438
Size: 501 2506 878 3677
Fragments arranged by size:
3677 2506 878 501
MslI CAynn'nnrTG
Cuts at: 770 1945 4451 5551 5678 770
Size: 1175 2506 1100 127 2654
Fragments arranged by size:
2654 2506 1175 1100 127
MspA1I CmG'CkG
Cuts at: 141 2257 2752 4190 4673 4987 5347 6360 6605
Size: 2116 495 1438 483 314 360 1013 245
Cuts at: 6605 141
Size: 1098
Fragments arranged by size:
2116 1438 1098 1013 495 483 360 314
245
NaeI GCC'GGC
Cuts at: 3755 4015 5743 3755
Size: 260 1728 5574
Fragments arranged by size:
5574 1728 260
NarI GG'CG_CC
Cuts at: 112 4017 4993 5241 112
Size: 3905 976 248 2433
Fragments arranged by size:
3905 2433 976 248
NcoI C'CATG_G
Cuts at: 107 1940 4256 107
Size: 1833 2316 3413
Fragments arranged by size:
3413 2316 1833
NdeI CA'TA_TG
Cuts at: 4452 4452
Size: 7562
NgoMIV G'CCGG_C
Cuts at: 3753 4013 5741 3753
Size: 260 1728 5574
Fragments arranged by size:
5574 1728 260
NsiI A_TGCA'T
Cuts at: 891 891
Size: 7562
NspI r_CATG'y
Cuts at: 4615 5115 6022 4615
Size: 500 907 6155
Fragments arranged by size:
6155 907 500
PciI A'CATG_T
Cuts at: 6018 6018
Size: 7562
PfoI T'CCnGG_A
Cuts at: 2681 2768 2865 3575 3595 4120 2681
Size: 87 97 710 20 525 6123
Fragments arranged by size:
6123 710 525 97 87 20
PmeI GTTT'AAAC
Cuts at: 4561 4561
Size: 7562
PmlI CAC'GTG
Cuts at: 3606 3606
Size: 7562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6701 6733 6701
Size: 32 7530
PpuMI rG'GwC_Cy
Cuts at: 1493 1493
Size: 7562
PstI C_TGCA'G
Cuts at: 1530 2430 4002 4145 4609 5294 1530
Size: 900 1572 143 464 685 3798
Fragments arranged by size:
3798 1572 900 685 464 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7562
PvuII CAG'CTG
Cuts at: 2257 2752 4190 4987 5347 2257
Size: 495 1438 797 360 4472
Fragments arranged by size:
4472 1438 797 495 360
RsrII CG'GwC_CG
Cuts at: 5757 5757
Size: 7562
SacI G_AGCT'C
Cuts at: 1644 4120 4576 1644
Size: 2476 456 4630
Fragments arranged by size:
4630 2476 456
SalI G'TCGA_C
Cuts at: 4599 4599
Size: 7562
SapI GCTCTTCn'nnn_
Cuts at: 178 2629 2854 5585 5795 178
Size: 2451 225 2731 210 1945
Fragments arranged by size:
2731 2451 1945 225 210
SbfI CC_TGCA'GG
Cuts at: 4609 4609
Size: 7562
ScaI AGT'ACT
Cuts at: 270 7079 270
Size: 6809 753
SfcI C'TryA_G
Cuts at: 33 1526 2344 2426 3041 3998 4141 4605 5290
Size: 1493 818 82 615 957 143 464 685
Cuts at: 5290 6283 6474 33
Size: 993 191 1121
Fragments arranged by size:
1493 1121 993 957 818 685 615 464
191 143 82
SfoI GGC'GCC
Cuts at: 113 4018 4994 5242 113
Size: 3905 976 248 2433
Fragments arranged by size:
3905 2433 976 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7562
SmaI CCC'GGG
Cuts at: 211 3355 4022 4584 211
Size: 3144 667 562 3189
Fragments arranged by size:
3189 3144 667 562
SmlI C'TyrA_G
Cuts at: 496 1819 1956 2028 4717 5632 6124 6386 496
Size: 1323 137 72 2689 915 492 262 1672
Fragments arranged by size:
2689 1672 1323 915 492 262 137 72
SpeI A'CTAG_T
Cuts at: 67 2793 67
Size: 2726 4836
SphI G_CATG'C
Cuts at: 4615 5115 4615
Size: 500 7062
SspI AAT'ATT
Cuts at: 1744 1744
Size: 7562
StuI AGG'CCT
Cuts at: 199 199
Size: 7562
StyI C'CwwG_G
Cuts at: 107 919 1489 1940 2783 3435 4058 4256 4696
Size: 812 570 451 843 652 623 198 440
Cuts at: 4696 5050 107
Size: 354 2619
Fragments arranged by size:
2619 843 812 652 623 570 451 440
354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3072 4463 5145 5278 5737 5946 3072
Size: 1391 682 133 459 209 4688
Fragments arranged by size:
4688 1391 682 459 209 133
TatI w'GTAC_w
Cuts at: 268 7077 268
Size: 6809 753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1284 1316 7079 7111 1284
Size: 32 5763 32 1735
Fragments arranged by size:
5763 1735 32 32
Tth111I GACn'n_nGTC
Cuts at: 5359 5359
Size: 7562
XbaI T'CTAG_A
Cuts at: 1203 1692 4593 1203
Size: 489 2901 4172
Fragments arranged by size:
4172 2901 489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 1861 2944 3779 5057 450
Size: 1411 1083 835 1278 2955
Fragments arranged by size:
2955 1411 1278 1083 835
XmaI C'CCGG_G
Cuts at: 209 3353 4020 4582 209
Size: 3144 667 562 3189
Fragments arranged by size:
3189 3144 667 562
XmnI GAAnn'nnTTC
Cuts at: 380 2764 380
Size: 2384 5178
ZraI GAC'GTC
Cuts at: 2330 2330
Size: 7562
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI ApaI AscI BaeI BmtI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FspAI MfeI NheI NotI NruI PacI PasI PflMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI